Motion responses of two ships advancing parallel in waves with hydrodynamic interactions are investigated in this paper. Within the framework of the frequency-domain potential flow theory, a semi-analytical higher-ord...Motion responses of two ships advancing parallel in waves with hydrodynamic interactions are investigated in this paper. Within the framework of the frequency-domain potential flow theory, a semi-analytical higher-order translating-pulsating source(HOTP) method is presented to solve the problems of coupled radiation and diffraction potential. The method employs nine-node bi-quadratic curvilinear elements to discretize the boundary integral equations(BIEs) constructed over the mean wetted surface of the two ship hulls. In order to eliminate the numerical oscillation, analytical quadrature formulas are derived and adopted to evaluate the integrals related to the Froudedependent part of the Green’s function along the horizontal direction in the BIEs. Based on the method, a numerical program is originally coded. Through the calculations of hydrodynamic responses of single ships, the numerical implementation is proved successful. Then the validated program is applied in the investigations on the hydrodynamic interactions of two identical Wigley Ⅲ hulls and the underway replenishment of a frigate and a supply ship in waves with and without stagger, respectively. The comparison between the present computed results with experimental data and numerical solutions of other methods shows that the semi-analytical HOTP method is of higher accuracy than the pulsating source Green’s function method with speed correction and better stability than the traditional HOTP method based on Gauss quadrature. In addition, for two ships with obviously different dimensions,the influence of hydrodynamic interactions on the smaller ship is found to be more noticeable than that on the larger ship, which leads to the differences between the motions of frigate with and without the presence of supply ship.展开更多
Obejctive To clone the full length of a differentially expressed cDNA fragment, LC27, and study its biological function tentatively Methods Northern blot was used to analyze the expression pattern of LC27 in hep...Obejctive To clone the full length of a differentially expressed cDNA fragment, LC27, and study its biological function tentatively Methods Northern blot was used to analyze the expression pattern of LC27 in hepatocellular carcinoma, matched nontumor liver tissues, fetal liver and normal adult liver tissues, as well as BEL 7402 hepatocellular carcinoma cell line ESTs splicing and 5' rapid amplification of cDNA ends (5' RACE) were used to clone the full length of LC27 cDNA An antisense oligodeoxynucleotide approach was used to investigate the biological role of the gene in the proliferation of BEL 7402 cells Results A 2186?bp novel cDNA with an open reading frame encoding a 283 amino acid protein was cloned Analysis of the deduced amino acid sequence indicated that it is 38% (88/229) identical to human Golgi 4 transmembrane spanning transporter MTP The gene and the encoded protein was termed hepatocellular carcinoma overexpressed transmembrane protein (hotp) and HOTP, respectively Hotp mRNA was almost undetectable in normal adult liver and fetal liver tissues However, it was significantly up regulated in hepatocellular carcinoma and some matched nontumor liver tissues, as well as BEL 7402 cells The proliferation of BEL 7402 cells was suppressed by an antisense oligodeoxynucleotide against hotp mRNA at a concentration of 50?μg/ml Conclusion HOTP may be an integral membrane transporter protein The overexpression of the gene in hepatocellular carcinoma may play an important role in hepatocarcinogenesis and disease progression展开更多
基金This work was financially supported by the National Natural Science Foundation of China(Grant No.52101357)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(Grant No.21KJB580012)the Scientific Research Start-up Fund of Jiangsu University of Science and Technology.
文摘Motion responses of two ships advancing parallel in waves with hydrodynamic interactions are investigated in this paper. Within the framework of the frequency-domain potential flow theory, a semi-analytical higher-order translating-pulsating source(HOTP) method is presented to solve the problems of coupled radiation and diffraction potential. The method employs nine-node bi-quadratic curvilinear elements to discretize the boundary integral equations(BIEs) constructed over the mean wetted surface of the two ship hulls. In order to eliminate the numerical oscillation, analytical quadrature formulas are derived and adopted to evaluate the integrals related to the Froudedependent part of the Green’s function along the horizontal direction in the BIEs. Based on the method, a numerical program is originally coded. Through the calculations of hydrodynamic responses of single ships, the numerical implementation is proved successful. Then the validated program is applied in the investigations on the hydrodynamic interactions of two identical Wigley Ⅲ hulls and the underway replenishment of a frigate and a supply ship in waves with and without stagger, respectively. The comparison between the present computed results with experimental data and numerical solutions of other methods shows that the semi-analytical HOTP method is of higher accuracy than the pulsating source Green’s function method with speed correction and better stability than the traditional HOTP method based on Gauss quadrature. In addition, for two ships with obviously different dimensions,the influence of hydrodynamic interactions on the smaller ship is found to be more noticeable than that on the larger ship, which leads to the differences between the motions of frigate with and without the presence of supply ship.
基金ThisworkwassupportedbygrantsfromtheNationalNaturalScienceFoundationofChina (No 39780 0 38and 3980 0 0 76 )
文摘Obejctive To clone the full length of a differentially expressed cDNA fragment, LC27, and study its biological function tentatively Methods Northern blot was used to analyze the expression pattern of LC27 in hepatocellular carcinoma, matched nontumor liver tissues, fetal liver and normal adult liver tissues, as well as BEL 7402 hepatocellular carcinoma cell line ESTs splicing and 5' rapid amplification of cDNA ends (5' RACE) were used to clone the full length of LC27 cDNA An antisense oligodeoxynucleotide approach was used to investigate the biological role of the gene in the proliferation of BEL 7402 cells Results A 2186?bp novel cDNA with an open reading frame encoding a 283 amino acid protein was cloned Analysis of the deduced amino acid sequence indicated that it is 38% (88/229) identical to human Golgi 4 transmembrane spanning transporter MTP The gene and the encoded protein was termed hepatocellular carcinoma overexpressed transmembrane protein (hotp) and HOTP, respectively Hotp mRNA was almost undetectable in normal adult liver and fetal liver tissues However, it was significantly up regulated in hepatocellular carcinoma and some matched nontumor liver tissues, as well as BEL 7402 cells The proliferation of BEL 7402 cells was suppressed by an antisense oligodeoxynucleotide against hotp mRNA at a concentration of 50?μg/ml Conclusion HOTP may be an integral membrane transporter protein The overexpression of the gene in hepatocellular carcinoma may play an important role in hepatocarcinogenesis and disease progression
