目的:探讨HOXA远端转录本(HOXA transcript at the distal tip,HOTTIP)在食管鳞癌(esophageal squamous cell cancer,ESCC)组织中的表达及对食管癌细胞部分生物学行为的影响。方法:采用逆转录-实时定量PCR法(RT-q PCR)检测ESCC组织及细...目的:探讨HOXA远端转录本(HOXA transcript at the distal tip,HOTTIP)在食管鳞癌(esophageal squamous cell cancer,ESCC)组织中的表达及对食管癌细胞部分生物学行为的影响。方法:采用逆转录-实时定量PCR法(RT-q PCR)检测ESCC组织及细胞中HOTTIP的表达水平;通过RNA干扰技术将si-HOTTIP转入细胞中以降低HOTTIP的表达水平,并通过定量PCR检测其干扰效率。用CCK8法和Transwell法检测敲除HOTTIP对细胞增殖能力和侵袭能力的影响。结果:与癌旁组织相比,ESCC组织中HOTTIP的表达明显升高;与正常食管上皮细胞相比,ESCC细胞中HOTTIP的表达明显升高。此外,通过分析临床数据发现高表达的HOTTIP与肿瘤体积和淋巴结转移紧密相关。CCK8和transwell实验显示,在敲除HOTTIP后,Eca-109和TE-13细胞的增殖和侵袭能力明显下降。结论:HOTTIP在ESCC细胞的增殖和侵袭过程中具有相当重要的作用。展开更多
HOXA transcript at the distal tip(HOTTIP),a newly identified long noncoding RNA,has been shown to exhibit anti-inflammatory effects and inhibit oxygen-glucose deprivation-induced neuronal apoptosis.However,its role in...HOXA transcript at the distal tip(HOTTIP),a newly identified long noncoding RNA,has been shown to exhibit anti-inflammatory effects and inhibit oxygen-glucose deprivation-induced neuronal apoptosis.However,its role in Parkinson's disease(PD)remains unclear.1-Methyl-4-phenylpyridium(MPP+)and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)were used to establish PD models in SH-SY5 Y and BV2 cells and in C57 BL/6 male mice,respectively.In vitro,after HOTTIP knockdown by sh-HOTTIP transfection,HOTTIP and FOXO1 overexpression promoted SH-SY5 Y apoptosis,BV2 microglial activation,proinflammatory cytokine expression,and nuclear factor kappa-B and NACHT,LRR and PYD domains-containing protein 3 inflammasome activation.Overexpression of mi R-615-3 p inhibited MPP+-induced neuronal apoptosis and microglial inflammation and ameliorated HOTTIP-and FOXO1-mediated nerve injury and inflammation.In vivo,HOTTIP knockdown alleviated motor dysfunction in PD mice and reduced neuronal apoptosis and microglial activation in the substantia nigra.These findings suggest that inhibition of HOTTIP mitigates neuronal apoptosis and microglial activation in PD models by modulating mi R-615-3 p/FOXO1.This study was approved by the Ethics Review Committee of the Affiliated Hospital of Qingdao University,China(approval No.UDX-2018-042)in June 2018.展开更多
The occurrence and development of digestive tract tumors are mainly caused by the interaction of genetic and environmental factors, multiple incentives, multiple genes involved, and multi-step regulation. With the dev...The occurrence and development of digestive tract tumors are mainly caused by the interaction of genetic and environmental factors, multiple incentives, multiple genes involved, and multi-step regulation. With the development of gene sequencing technology, precise treatment of tumor era has arrived. Studies have shown that the HOXA13 gene in the homeobox gene is abnormally expressed in digestive system tumors. Studies have shown that HOXA13 may have a certain relationship with the occurrence, development and prognosis of the tumor, pending the diagnosis and treatment of digestive tract tumors with a new gene target.展开更多
文摘目的:探讨HOXA远端转录本(HOXA transcript at the distal tip,HOTTIP)在食管鳞癌(esophageal squamous cell cancer,ESCC)组织中的表达及对食管癌细胞部分生物学行为的影响。方法:采用逆转录-实时定量PCR法(RT-q PCR)检测ESCC组织及细胞中HOTTIP的表达水平;通过RNA干扰技术将si-HOTTIP转入细胞中以降低HOTTIP的表达水平,并通过定量PCR检测其干扰效率。用CCK8法和Transwell法检测敲除HOTTIP对细胞增殖能力和侵袭能力的影响。结果:与癌旁组织相比,ESCC组织中HOTTIP的表达明显升高;与正常食管上皮细胞相比,ESCC细胞中HOTTIP的表达明显升高。此外,通过分析临床数据发现高表达的HOTTIP与肿瘤体积和淋巴结转移紧密相关。CCK8和transwell实验显示,在敲除HOTTIP后,Eca-109和TE-13细胞的增殖和侵袭能力明显下降。结论:HOTTIP在ESCC细胞的增殖和侵袭过程中具有相当重要的作用。
文摘HOXA transcript at the distal tip(HOTTIP),a newly identified long noncoding RNA,has been shown to exhibit anti-inflammatory effects and inhibit oxygen-glucose deprivation-induced neuronal apoptosis.However,its role in Parkinson's disease(PD)remains unclear.1-Methyl-4-phenylpyridium(MPP+)and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)were used to establish PD models in SH-SY5 Y and BV2 cells and in C57 BL/6 male mice,respectively.In vitro,after HOTTIP knockdown by sh-HOTTIP transfection,HOTTIP and FOXO1 overexpression promoted SH-SY5 Y apoptosis,BV2 microglial activation,proinflammatory cytokine expression,and nuclear factor kappa-B and NACHT,LRR and PYD domains-containing protein 3 inflammasome activation.Overexpression of mi R-615-3 p inhibited MPP+-induced neuronal apoptosis and microglial inflammation and ameliorated HOTTIP-and FOXO1-mediated nerve injury and inflammation.In vivo,HOTTIP knockdown alleviated motor dysfunction in PD mice and reduced neuronal apoptosis and microglial activation in the substantia nigra.These findings suggest that inhibition of HOTTIP mitigates neuronal apoptosis and microglial activation in PD models by modulating mi R-615-3 p/FOXO1.This study was approved by the Ethics Review Committee of the Affiliated Hospital of Qingdao University,China(approval No.UDX-2018-042)in June 2018.
文摘The occurrence and development of digestive tract tumors are mainly caused by the interaction of genetic and environmental factors, multiple incentives, multiple genes involved, and multi-step regulation. With the development of gene sequencing technology, precise treatment of tumor era has arrived. Studies have shown that the HOXA13 gene in the homeobox gene is abnormally expressed in digestive system tumors. Studies have shown that HOXA13 may have a certain relationship with the occurrence, development and prognosis of the tumor, pending the diagnosis and treatment of digestive tract tumors with a new gene target.
