[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions neede...[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS ＋ BA 0.3 mg/L ＋2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS ＋ AgNO31.5 mg/L ＋ BA 0.4 mg/L ＋ NAA 0.1 mg/L. 1/2 MS ＋ IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.展开更多
From the ethanolic extract of the whole plant of Hemistepta lyrata Bunge., a new lignan, named hemislin B glucoside, has been isolated and identified as a-(trans-3-methoxy-4-O-b-D-glucopyranosylbenzylidene)-b-(3,4-dim...From the ethanolic extract of the whole plant of Hemistepta lyrata Bunge., a new lignan, named hemislin B glucoside, has been isolated and identified as a-(trans-3-methoxy-4-O-b-D-glucopyranosylbenzylidene)-b-(3,4-dimethoxybenzyl)-g-butyrolactone. Its structure has been established on the basis of UV, IR, FABMS, 1H-NMR, 13C-NMR, HMQC and HMBC spectral analysis.展开更多
文摘[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS ＋ BA 0.3 mg/L ＋2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS ＋ AgNO31.5 mg/L ＋ BA 0.4 mg/L ＋ NAA 0.1 mg/L. 1/2 MS ＋ IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.
文摘From the ethanolic extract of the whole plant of Hemistepta lyrata Bunge., a new lignan, named hemislin B glucoside, has been isolated and identified as a-(trans-3-methoxy-4-O-b-D-glucopyranosylbenzylidene)-b-(3,4-dimethoxybenzyl)-g-butyrolactone. Its structure has been established on the basis of UV, IR, FABMS, 1H-NMR, 13C-NMR, HMQC and HMBC spectral analysis.
