Background: Purpose of this study was to evaluate various parameters of meningioma after surgical treatment and analyze predictive factors for recurrence. Methods: During 1992-2007, 353 patients were operated for intr...Background: Purpose of this study was to evaluate various parameters of meningioma after surgical treatment and analyze predictive factors for recurrence. Methods: During 1992-2007, 353 patients were operated for intracranial meningioma in our department. They were followed since, and related data were combined with parameters such as tumor histology (WHO system), tumor location and the extent of tumor resection (Simpson’s scale). The results were analyzed with respect to tumor reappearance. This study was approved by the ethics committee of G. Papanikolaou Hospital. Results: The mean follow up period was 6.45 years. The overall percentage of recurrence was 21.52%. Grade 1 meningiomas (benign) recurred at a rate of 19.1%, grade 2 tumors (atypical) showed 41.7% rate of recurrence and grade 3 meningiomas (malignant) recurred at a rate of 75%. Tumor histopathology was not significant to recurrence (p > 0.001). The location of meningiomas was not found to be significant to recurrence (p > 0.001). Complete tumor resection was accomplished in 269 patients (76.2%). Incomplete resection (Simpson grades 2-5) took place in 84 cases (23.8%). The recurrence rate after complete resection was 13.8%, while the rate for cases with incomplete resection was 46.7%. The extent of removal was significantly associated with recurrence (p < 0.001). Conclusions: Tumor recurrence was observed at 21.5% of patients in our series of intracranial meningioma surgery. The rate of recurrence was related primarily to the extent of surgical removal. Neither tumor site, nor tumor histology were predictive factors for recurrence (ma-lignant meningiomas excluded).展开更多
Objective: To study the significance of histological grading as a prognostic factor in ductal carcinoma in situ of the breast. Methods: According to the Van Nuy’s classification, 32 cases of ductal carcinoma in situ ...Objective: To study the significance of histological grading as a prognostic factor in ductal carcinoma in situ of the breast. Methods: According to the Van Nuy’s classification, 32 cases of ductal carcinoma in situ (DCIS) of the breast were divided into three groups. Results: Low grade (well differentiated, low grade DCIS) 12 patients (37.5%); Intermediate grade, 9 patients (28.1%); High grade (poorly differentiated DCIS) 11 patients (34.4%). Among the high grade DCIS, the histologic subtypes were comedo (9 patients), micropapillary (1 patient) and solid (1 patient). The positive expression of c-erbB-2, p53 and MIB-1 in high grade DCIS was higher than that in intermediate and low grade DCIS. The difference between high grade and low grade DCIS was significant (p<0.05). The expression of ER in high grade DCIS was lower than that in intermediate and low grade DCIS. Conclusions: Histological grading of breast ductal carcinoma in situ may be a good prognostic factor.展开更多
The importance of the accuracy of preparing biological specimen as histological sections that can be examined under a microscope lies in reflecting a true image of the tissue that includes all its components, which ar...The importance of the accuracy of preparing biological specimen as histological sections that can be examined under a microscope lies in reflecting a true image of the tissue that includes all its components, which are used in scientific research or for the purpose of diagnosing various diseases of the body. Despite this, some cellular structures within the tissue may suffer from some alterations that result from the appearance of defects during any stage of preparing these microscopic sections, which alter or interfere with the precise cellular structures and morphology that constitute the tissue and thus give a different image for tissue features and cause confusion in the work histopathologist in the diagnosis. There are several reasons that can cause a misdiagnosis of the sample that occurs during the surgical separation process or after separation during the stages of microscopic preparation techniques from fixation stage, tissue processing, embedding or microtomy, staining until mounting procedures. The constant need to identify these defects and their causes in addition to try to reduce them is one of the biggest challenges evident in pathology laboratories. Therefore, this study aims to review the most common defects that occur in any stage of tissue processing, with an explanation of their causes and appropriate ways to avoid them.展开更多
Gibberellin(GA)is one of the major plant hormones that promote parthenocarpy,a highly valuable agronomic trait.Here,we demonstrated that exogenous GA3application triggered the formation of parthenocarpic fruits with s...Gibberellin(GA)is one of the major plant hormones that promote parthenocarpy,a highly valuable agronomic trait.Here,we demonstrated that exogenous GA3application triggered the formation of parthenocarpic fruits with smaller size but unchanged shape in tomato(Solanum lycopersicum).These fruits exhibited a thicker pericarp,undeveloped ovules,and few jelly tissues,leading to smaller locules with empty cavities.Histological investigation showed that GA treatment produced more cell layers with larger cells in the pericarp,suggesting its promotion in both cell division and expansion.Transcriptomic analyses between GA-3and mock-treated unpollinated ovaries/fruits identified a large number of differentially expressed genes related to hormones,cell division,cell expansion,and transcription factors,implying that they coordinately regulated parthenocarpy conferred by GA.In particular,the downregulation of five reported repressors of tomato parthenocarpy,including two auxin signaling components,AUXIN RESPONSE FACTOR5(SlARF5)and Sl ARF7,and three MADS-box genes,TOMATO APETALA3(TAP3),TOMATO PISTILLATA(TPI),and AGAMOUS-LIKE6(SlAGL6),after GA treatment might play a key role in this process.Furthermore,we found that the knockdown of a GA signaling factor SlMYB33,which was depressed by GA treatment,induced parthenocarpic fruit set in tomato,an effect that might have been achieved by enhancing GA biosynthesis and decreasing the expression of some repressors of tomato parthenocarpy.Thus,our results provide a basis for understanding the regulatory mechanism of GA in tomato parthenocarpy.展开更多
Distraction spinal cord injury is caused by some degree of distraction or longitudinal tension on the spinal cord and commonly occurs in patients who undergo corrective operation for severe spinal deformity.With the i...Distraction spinal cord injury is caused by some degree of distraction or longitudinal tension on the spinal cord and commonly occurs in patients who undergo corrective operation for severe spinal deformity.With the increased degree and duration of distraction,spinal cord injuries become more serious in terms of their neurophysiology,histology,and behavior.Very few studies have been published on the specific characteristics of distraction spinal cord injury.In this study,we systematically review 22 related studies involving animal models of distraction spinal cord injury,focusing particularly on the neurophysiological,histological,and behavioral characteristics of this disease.In addition,we summarize the mechanisms underlying primary and secondary injuries caused by distraction spinal cord injury and clarify the effects of different degrees and durations of distraction on the primary injuries associated with spinal cord injury.We provide new concepts for the establishment of a model of distraction spinal cord injury and related basic research,and provide reference guidelines for the clinical diagnosis and treatment of this disease.展开更多
Introduction: The frequent late-stage diagnosis of chronic kidney disease (CKD) constrains the treatment choices for nephrologists. Renal biopsy (RB) is crucial for accurately identifying renal lesions. This key nephr...Introduction: The frequent late-stage diagnosis of chronic kidney disease (CKD) constrains the treatment choices for nephrologists. Renal biopsy (RB) is crucial for accurately identifying renal lesions. This key nephrological procedure, however, is invasive and not without potential complications. The purpose of this study was to evaluate the indications, frequency, and histological lesion profiles of renal biopsies in Togo. Materials and Methods: We conducted a descriptive cross-sectional study encompassing all renal biopsies performed in Togo from the inception of nephrology services to the present. Data were compiled from the medical records of the patients. Results: From 2015 to 2023, 68 high-quality renal biopsies were executed in Togo. The patients had an average age of 30.6 years, with a predominance of males (69.1%). The most common indication was nephrotic syndrome, accounting for 66.2% of cases. Histologically, glomerulopathies were predominant, representing 61.8% of lesions, followed by vascular nephropathies (25%) and tubulointerstitial nephropathies (13.2%). The most frequently observed primary glomerulopathy was focal segmental glomerulosclerosis (FSGS). Gross hematuria was the sole complication, occurring in 1.4% of the cases. Conclusion: RB is an evolving practice in Togo. Glomerulopathies are the most commonly observed lesions. The histological categorization of renal lesions is vital for clinicians in their diagnostic reasoning and approach.展开更多
Objective: To investigate histo-pathological distribution and clinico-pathological significance in a large Chinese triple-negative breast cancer(TNBC) patients serials based on the latest understanding of its clinico-...Objective: To investigate histo-pathological distribution and clinico-pathological significance in a large Chinese triple-negative breast cancer(TNBC) patients serials based on the latest understanding of its clinico-pathological diversity, and to provide more information to clinicians to improve precision of individualized treatment of TNBC.Methods: A retrospective analysis was performed on patients with TNBC at Breast Disease Center, Peking University First Hospital between January 2010 and December 2019. Histo-and clinico-pathological characteristics were analyzed by Chi-square test and Student's t-test, and prognoses were calculated using KaplanMeier method and a Cox proportionate hazards model. Bonferroni correction was used to correct for multiple comparison.Results: Conventional type of TNBC(c TNBC) were identified in 73.7% of 582 TNBC, while special type of TNBC(s TNBC) were 26.3%, including 71 apocrine carcinoma, 20 medullary carcinoma, 31 metaplastic carcinoma, 18 invasive lobular carcinoma, 7 invasive micropapillary carcinoma, 5 adenoid cystic carcinoma and 1 acinic cell carcinoma. Compared to s TNBC, c TNBC was associated with high histologic grade(P<0.001) and lower androgen receptor(AR) expression(P<0.001). TNM stage of low-grade c TNBC was significantly lower than that of high-grade c TNBC(P=0.002). Although no significant difference, there was a trend that the rate of 5-year disease-free survival(DFS) and 5-year overall survival(OS) were longer in high-grade c TNBC than in high-grade s TNBC(P=0.091 and 0.518), and were longer in low-grade s TNBC than in high-grade s TNBC(P=0.051 and0.350). Metaplastic carcinomas showed larger tumor size(P=0.008) and higher proliferative Ki67 index(P=0.004)than c TNBCs.Conclusions: Results from our cohort imply that sub-categorization or subtyping and histological grading could be meaningful in pathological evaluation of TNBC, and need to be clarified in more large collections of TNBC.展开更多
Response criteria remain controversial in therapeutic evaluation for locally advanced esophageal carcinoma treated with neoadjuvant chemotherapy.We aimed to identify the predictive value of tumor regression grading(TR...Response criteria remain controversial in therapeutic evaluation for locally advanced esophageal carcinoma treated with neoadjuvant chemotherapy.We aimed to identify the predictive value of tumor regression grading(TRG) in tumor response and prognosis.Fifty-two patients who underwent neoadjuvant chemotherapy followed by esophagectomy and radical 2-field lymphadenectomy between June 2007 and June 2011 were included in this study.All tissue specimens were reassessed according to the TRG scale.Potential prognostic factors,including clinicopathologic factors,were evaluated.Survival curves were generated by using the Kaplan-Meier method and compared with the log-rank test.Prognostic factors were determined with multivariate analysis by using the Cox regression model.Our results showed that of 52 cases,43(83%) were squamous cell carcinoma and 9(17%) were adenocarcinoma.TRG was correlated with pathologic T(P = 0.006) and N(P < 0.001) categories.Median overall survival for the entire cohort was 33 months.The 1-and 2-year overall survival rates were 71% and 44%,respectively.Univariate survival analysis results showed that favorable prognostic factors were histological subtype(P = 0.003),pathologic T category(P = 0.026),pathologic N category(P < 0.001),and TRG G0(P = 0.041).Multivariate analyses identified pathologic N category(P < 0.001) as a significant independent prognostic parameter.Our results indicate that histomorphologic TRG can be considered as an alternative option to predict the therapeutic efficacy and prognostic factor for patients with locally advanced esophageal carcinoma treated by neoadjuvant chemotherapy.展开更多
The environment significantly impacts the interaction between plants and pathogens,thus remarkably affecting crop disease occurrence.However,the detailed combined mechanisms of temperature and humidity influencing thi...The environment significantly impacts the interaction between plants and pathogens,thus remarkably affecting crop disease occurrence.However,the detailed combined mechanisms of temperature and humidity influencing this interaction remain unclear.In this study,the interaction between tomato and Botrytis cinerea in various temperature and humidity conditions was analyzed by histological observation and a dual RNA-seq approach.Results showed that low humidity was not favorable for mycelial growth,resulting in infection failure.Both high and low temperatures at high humidity successfully inhibited pathogenic infection and disease incidence in the tomato plants,thus enhancing their resistance to B.cinerea.The high temperature and high humidity(HH)treatment induced the upregulation of light reaction genes,increased the net photosynthetic rate,and expanded the chloroplast morphology of infected tomatoes.The HH treatment also inhibited the expression of cell cycle-related genes of B.cinerea,interfered with conidial germination andmycelial growth,and damagedmycelial cell structure.Lowtemperature and high humidity(LH)treatment induced the expression of cell wall modification genes and remodeled the cell wall morphology of tomatoes in response to B.cinerea.In addition,the downregulated fungal catabolic genes and the abnormal increase in electron density ofmycelial cells under LH treatment subsequently reduced the infection ability of B.cinerea.These results further explain the coupled effects of temperature and humidity on plant defenses and pathogen virulence,and provide a potential means to control gray mold.展开更多
BACKGROUND Histological remission is increasingly accepted as a treatment endpoint in the management of ulcerative colitis(UC).However,the knowledge of histology guidelines and the attitudes towards their use in clini...BACKGROUND Histological remission is increasingly accepted as a treatment endpoint in the management of ulcerative colitis(UC).However,the knowledge of histology guidelines and the attitudes towards their use in clinical practice by gastroenterologists and pathologists is unknown.AIM To evaluate the knowledge of histology guidelines and attitudes towards the use of histology in UC by gastroenterologists and pathologists.METHODS A prospective,cross-sectional nationwide survey of gastroenterologists and pathologists who analyse UC specimens was conducted.The survey consisted of 34 questions to assess gastroenterologists’and pathologists’knowledge(score out of 19)and attitudes towards histological assessment in UC.Survey questions were formulated using the European Crohn’s and Colitis position paper on histopathology and the British Society of Gastroenterology biopsy reporting guidelines.It included knowledge of histological assessment of disease activity and dysplasia,knowledge of histological scoring systems for ulcerative colitis,uptake of histology scoring systems in routine practice,attitudes towards the role of histological activity,and the use of histological activity in clinical scenarios.RESULTS Of 89 responders(77 gastroenterologists,12 pathologists),there was almost universal acceptance that histological assessment should form part of UC evaluation[95%gastroenterologists,92%pathologists].However,gastroenterologists reported that 92%of their pathologists do not use a histological scoring system.Utilisation of a formal histological scoring system was preferred by 77%of gastroenterologists and 58%of pathologists.Both groups lacked awareness of the Geboes Score,Nancy Index and Robarts Histopathological Index scoring systems with 91%,87%,and 92%of gastroenterologists respectively;and 83%,83%,and 92%pathologists respectively,being uncertain of scoring systems’remission definitions.Histology knowledge score was not significantly different between gastroenterologists and pathologists[9/19(IQR:8-11)vs 8/19(IQR:7-10),P=0.54].Higher knowledge scores were predicted by hospital attending gastroenterologists(P=0.004),participation in inflammatory bowel disease(IBD)multidisciplinary teams(P=0.009),and self-declared IBD sub-specialist(P=0.03).CONCLUSION Histological remission is a recognised target for both gastroenterologists and pathologists.Despite this,knowledge of histological scoring systems and their utilisation is poor.展开更多
Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particul...Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particularly the benthic organisms.How-ever,the toxic effects of PFOA on the half-smooth tongue sole Cynoglossus semilaevis,a commercial benthic fish in China,have rarely been reported.Because juvenile fish are sensitive to environmental pollutants,in the present study,histological assessment and tran-scriptome sequencing were performed to determine the short-term impact of PFOA on juvenile half-smooth tongue soles.Histologi-cal analysis showed that PFOA exposure caused hepatocyte rupture,intestinal villi breakage,increased goblet cell count,and brain ab-normal.Transcriptome results showed that some interesting signaling pathways,such as glycolysis/gluconeogenesis,PPAR signaling pathway and GABAergic synapse signaling pathway,were enriched after PFOA exposure.In addition,some metabolic,immune and neural genes were differentially expressed,which including ependymin,hbb1-like and gad 1,and they were up-regulated after 14 days of exposure.Transcriptome results also indicated that half-smooth tongue sole might improve energy metabolism in response to PFOA toxicity after 7 days of exposure.These findings provide a basis for studying the ecological effects of PFOA on marine benthic fishes.展开更多
This paper reports the application of multi-component hydrocracking catalyst grading technology in diesel hydrocracking system to increase naphtha,and studies the influence of catalyst systems with different number of...This paper reports the application of multi-component hydrocracking catalyst grading technology in diesel hydrocracking system to increase naphtha,and studies the influence of catalyst systems with different number of graded beds on the reaction process of diesel hydrocracking.Three hydrocracking catalysts with different physicochemical properties as gradation components,the diesel hydrocracking reaction on catalyst systems of one-component,two-component and three-component graded beds with different loading sequences are carried out and evaluated,respectively.The catalytic mechanism of the multi-component grading system is analyzed.The results show that,with the increase of the number of grading beds,the space velocity of reaction on each catalyst increases,which can effectively control the overreaction process;along the flow direction of feedstock,the loading sequences of catalysts with acidity decreasing and pore properties increasing can satisfy the demand of different catalytic activity for the conversion of reactant with changing composition to naphtha,which has a guiding role in the conversion of feedstock to target products.Therefore,the conversion of diesel,the selectivity and yield of naphtha all increase significantly on the multi-component catalyst system.The research on the grading technology of multi-component catalysts is of great significance to the promotion and application of catalyst systems in various catalytic fields.展开更多
Purpose: This study investigated the anatomical and histological characteristics of the rat Eustachian tube(E-tube)and the feasibility of Eustachian tubography in a rat model.Materials and methods: Fifteen male Wistar...Purpose: This study investigated the anatomical and histological characteristics of the rat Eustachian tube(E-tube)and the feasibility of Eustachian tubography in a rat model.Materials and methods: Fifteen male Wistar rats were used in this study, and the bilateral E-tubes of each rat were examined. Ten E-tubes were used for anatomical studies, another ten for histological analysis, and the other ten for Eustachian tubography. Five rats were euthanized and decapitated, and ten E-tubes were dissected to describe the anatomy of the E-tube. Ten E-tube specimens obtained from five other rats were sectioned to investigate Etube histology. Eustachian tubography was performed on the bilateral E-tubes of the other five rats using the trans-tympanic approach.Results: The rat E-tubes consisted of bony and membranous parts. Cartilage and bone tissue covered only the bony part. The E-tubes’ mean diameter and overall length were 2.97 mm and 4.96 mm, respectively. The tympanic orifices’ mean diameter was 1.21 mm. The epithelium of E-tubes was mainly composed of pseudostratified ciliated and goblet cells. Eustachian tubography was successfully performed on both sides of the E-tube for each rat.The technical success rate was 100%, the average running time was 4.9 min, and no procedure-related complications occurred. On tubography images, the E-tube, tympanic cavity, and nasopharynx could be identified because of the visualization of bony landmarks.Conclusion: In this study, we described the anatomical and histological features of rat E-tubes. With the aid of these findings, E-tube angiography was successfully performed using a transtympanic approach. These results will facilitate further investigation of E-tube dysfunction.展开更多
Stains are applied to impart contrast to the tissue and identify particular features of interest. However, the use of synthetic dyes as staining reagents has been associated with significant human health challenges an...Stains are applied to impart contrast to the tissue and identify particular features of interest. However, the use of synthetic dyes as staining reagents has been associated with significant human health challenges and pollution of the ecosystem. These developments have necessitated a shift towards using natural dyes that are eco-friendlier and readily available. We investigated the staining reaction patterns of teak tree leaves (Tectona grandis) dye extracts and explored their suitability as a cytoplasmic stain in micromorphological assessments. Dye extracts were prepared using acetone, methanol, and ethanol as solvents from air-dried (under shade) teak tree young leaves. The dye extracts were applied as a counterstain and evaluated against eosin in formalin-fixed paraffin-embedded (FFPE) bovine tissue sections at varying concentrations and different staining times. Teak tree leaves (Tectona grandis) dye extracts produced relatively varying staining intensities of reddish-brown cytoplasmic coloration when used on bovine tissue at different concentrations and staining times comparable to eosin and with blue-purple hematoxylin nuclear stain. The present study showed that Tectona grandis leaf dye extracts provide an excellent cytoplasmic staining pattern and can be used as an alternative counterstain in routine H&E staining techniques.展开更多
BACKGROUND In Japan,the transhiatal approach,including lower mediastinal lymph node dissection,is widely performed for Siewert type II esophagogastric junction adenocarcinoma.This procedure is generally performed in a...BACKGROUND In Japan,the transhiatal approach,including lower mediastinal lymph node dissection,is widely performed for Siewert type II esophagogastric junction adenocarcinoma.This procedure is generally performed in a magnified view using laparoscopy or a robotic system,therefore,the microanatomy of the lower mediastinum is important.However,mediastinal microanatomy is still unclear and classification of lower mediastinal lymph nodes is not currently based on fascia or other microanatomical structures.AIM To clarify the fascia and layer structures of the lower mediastinum and classify the lower mediastinal tissue.METHODS We dissected the esophagus and surrounding organs en-bloc from seven cadavers fixed in 10%formalin.Organs and tissues were then cut at the level of the lower thoracic esophagus,embedded in paraffin,and serially sectioned.Tissue sections were stained with Hematoxylin-Eosin(all cadavers)and immunostained for the lymphatic endothelial marker D2-40(three cadavers).We observed the periesophageal fasciae and layers,and defined lymph node boundaries based on the fasciae.Lymphatic vessels around the esophagus were observed on immunostained tissue sections.RESULTS We identified two fasciae,A and B.We then classified lower mediastinal tissue into three areas,paraesophageal,paraaortic,and intermediate,using these fasciae as boundaries.Lymph nodes were found to be present and were counted in each area.The dorsal part of the intermediate area was thicker on the caudal side than on the cranial side in all cadavers.On the dorsal side,no blood vessels penetrated the fasciae in six of the seven cadavers,whereas the proper esophageal artery penetrated fascia B in one cadaver.D2-40 immunostaining showed lymphatic vessel connections between the paraesophageal and intermediate areas on the lateral and ventral sides of the esophagus,but no lymphatic connection between areas on the dorsal side of the esophagus.CONCLUSION Histological studies identified two fasciae surrounding the esophagus in the lower mediastinum and the layers separated by these fasciae were used to classify the lower mediastinal tissues.展开更多
Diabetes problems can lead to an eye disease called Diabetic Retinopathy(DR),which permanently damages the blood vessels in the retina.If not treated early,DR becomes a significant reason for blindness.To identify the...Diabetes problems can lead to an eye disease called Diabetic Retinopathy(DR),which permanently damages the blood vessels in the retina.If not treated early,DR becomes a significant reason for blindness.To identify the DR and determine the stages,medical tests are very labor-intensive,expensive,and timeconsuming.To address the issue,a hybrid deep and machine learning techniquebased autonomous diagnostic system is provided in this paper.Our proposal is based on lesion segmentation of the fundus images based on the LuNet network.Then a Refined Attention Pyramid Network(RAPNet)is used for extracting global and local features.To increase the performance of the classifier,the unique features are selected from the extracted feature set using Aquila Optimizer(AO)algorithm.Finally,the LightGBM model is applied to classify the input image based on the severity.Several investigations have been done to analyze the performance of the proposed framework on three publically available datasets(MESSIDOR,APTOS,and IDRiD)using several performance metrics such as accuracy,precision,recall,and f1-score.The proposed classifier achieves 99.29%,99.35%,and 99.31%accuracy for these three datasets respectively.The outcomes of the experiments demonstrate that the suggested technique is effective for disease identification and reliable DR grading.展开更多
To solve inefficient water stress classification of spinach seedlings under complex background,this study proposed an automatic classification method for the water stress level of spinach seedlings based on the N-Mobi...To solve inefficient water stress classification of spinach seedlings under complex background,this study proposed an automatic classification method for the water stress level of spinach seedlings based on the N-MobileNetXt(NCAM+MobileNetXt)network.Firstly,this study recon-structed the Sandglass Block to effectively increase the model accuracy;secondly,this study introduced the group convolution module and a two-dimensional adaptive average pool,which can significantly compress the model parameters and enhance the model robustness separately;finally,this study innovatively proposed the Normalization-based Channel Attention Module(NCAM)to enhance the image features obviously.The experimental results showed that the classification accuracy of N-MobileNetXt model for spinach seedlings under the natural environment reached 90.35%,and the number of parameters was decreased by 66%compared with the original MobileNetXt model.The N-MobileNetXt model was superior to other net-work models such as ShuffleNet and GhostNet in terms of parameters and accuracy of identification.It can provide a theoretical basis and technical support for automatic irrigation.展开更多
Cataract is the leading cause of visual impairment globally.The scarcity and uneven distribution of ophthalmologists seriously hinder early visual impairment grading for cataract patients in the clin-ic.In this study,...Cataract is the leading cause of visual impairment globally.The scarcity and uneven distribution of ophthalmologists seriously hinder early visual impairment grading for cataract patients in the clin-ic.In this study,a deep learning-based automated grading system of visual impairment in cataract patients is proposed using a multi-scale efficient channel attention convolutional neural network(MECA_CNN).First,the efficient channel attention mechanism is applied in the MECA_CNN to extract multi-scale features of fundus images,which can effectively focus on lesion-related regions.Then,the asymmetric convolutional modules are embedded in the residual unit to reduce the infor-mation loss of fine-grained features in fundus images.In addition,the asymmetric loss function is applied to address the problem of a higher false-negative rate and weak generalization ability caused by the imbalanced dataset.A total of 7299 fundus images derived from two clinical centers are em-ployed to develop and evaluate the MECA_CNN for identifying mild visual impairment caused by cataract(MVICC),moderate to severe visual impairment caused by cataract(MSVICC),and nor-mal sample.The experimental results demonstrate that the MECA_CNN provides clinically meaning-ful performance for visual impairment grading in the internal test dataset:MVICC(accuracy,sensi-tivity,and specificity;91.3%,89.9%,and 92%),MSVICC(93.2%,78.5%,and 96.7%),and normal sample(98.1%,98.0%,and 98.1%).The comparable performance in the external test dataset is achieved,further verifying the effectiveness and generalizability of the MECA_CNN model.This study provides a deep learning-based practical system for the automated grading of visu-al impairment in cataract patients,facilitating the formulation of treatment strategies in a timely man-ner and improving patients’vision prognosis.展开更多
The increasing trend towards independent fruit packaging demands a high appearance quality of individually packed fruits.In this paper,we propose an improved YOLOv5-based model,YOLO-Banana,to effectively grade banana ...The increasing trend towards independent fruit packaging demands a high appearance quality of individually packed fruits.In this paper,we propose an improved YOLOv5-based model,YOLO-Banana,to effectively grade banana appearance quality based on the number of banana defect points.Due to the minor and dense defects on the surface of bananas,existing detection algorithms have poor detection results and high missing rates.To address this,we propose a densitybased spatial clustering of applications with noise(DBSCAN)and K-means fusion clustering method that utilizes refined anchor points to obtain better initial anchor values,thereby enhancing the network’s recognition accuracy.Moreover,the optimized progressive aggregated network(PANet)enables better multi-level feature fusion.Additionally,the non-maximum suppression function is replaced with a weighted non-maximum suppression(weighted NMS)function based on distance intersection over union(DIoU).Experimental results show that the model’s accuracy is improved by 2.3%compared to the original YOLOv5 network model,thereby effectively grading the banana appearance quality.展开更多
文摘Background: Purpose of this study was to evaluate various parameters of meningioma after surgical treatment and analyze predictive factors for recurrence. Methods: During 1992-2007, 353 patients were operated for intracranial meningioma in our department. They were followed since, and related data were combined with parameters such as tumor histology (WHO system), tumor location and the extent of tumor resection (Simpson’s scale). The results were analyzed with respect to tumor reappearance. This study was approved by the ethics committee of G. Papanikolaou Hospital. Results: The mean follow up period was 6.45 years. The overall percentage of recurrence was 21.52%. Grade 1 meningiomas (benign) recurred at a rate of 19.1%, grade 2 tumors (atypical) showed 41.7% rate of recurrence and grade 3 meningiomas (malignant) recurred at a rate of 75%. Tumor histopathology was not significant to recurrence (p > 0.001). The location of meningiomas was not found to be significant to recurrence (p > 0.001). Complete tumor resection was accomplished in 269 patients (76.2%). Incomplete resection (Simpson grades 2-5) took place in 84 cases (23.8%). The recurrence rate after complete resection was 13.8%, while the rate for cases with incomplete resection was 46.7%. The extent of removal was significantly associated with recurrence (p < 0.001). Conclusions: Tumor recurrence was observed at 21.5% of patients in our series of intracranial meningioma surgery. The rate of recurrence was related primarily to the extent of surgical removal. Neither tumor site, nor tumor histology were predictive factors for recurrence (ma-lignant meningiomas excluded).
文摘Objective: To study the significance of histological grading as a prognostic factor in ductal carcinoma in situ of the breast. Methods: According to the Van Nuy’s classification, 32 cases of ductal carcinoma in situ (DCIS) of the breast were divided into three groups. Results: Low grade (well differentiated, low grade DCIS) 12 patients (37.5%); Intermediate grade, 9 patients (28.1%); High grade (poorly differentiated DCIS) 11 patients (34.4%). Among the high grade DCIS, the histologic subtypes were comedo (9 patients), micropapillary (1 patient) and solid (1 patient). The positive expression of c-erbB-2, p53 and MIB-1 in high grade DCIS was higher than that in intermediate and low grade DCIS. The difference between high grade and low grade DCIS was significant (p<0.05). The expression of ER in high grade DCIS was lower than that in intermediate and low grade DCIS. Conclusions: Histological grading of breast ductal carcinoma in situ may be a good prognostic factor.
文摘The importance of the accuracy of preparing biological specimen as histological sections that can be examined under a microscope lies in reflecting a true image of the tissue that includes all its components, which are used in scientific research or for the purpose of diagnosing various diseases of the body. Despite this, some cellular structures within the tissue may suffer from some alterations that result from the appearance of defects during any stage of preparing these microscopic sections, which alter or interfere with the precise cellular structures and morphology that constitute the tissue and thus give a different image for tissue features and cause confusion in the work histopathologist in the diagnosis. There are several reasons that can cause a misdiagnosis of the sample that occurs during the surgical separation process or after separation during the stages of microscopic preparation techniques from fixation stage, tissue processing, embedding or microtomy, staining until mounting procedures. The constant need to identify these defects and their causes in addition to try to reduce them is one of the biggest challenges evident in pathology laboratories. Therefore, this study aims to review the most common defects that occur in any stage of tissue processing, with an explanation of their causes and appropriate ways to avoid them.
基金supported by the Key Program of Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2021JZ-13)。
文摘Gibberellin(GA)is one of the major plant hormones that promote parthenocarpy,a highly valuable agronomic trait.Here,we demonstrated that exogenous GA3application triggered the formation of parthenocarpic fruits with smaller size but unchanged shape in tomato(Solanum lycopersicum).These fruits exhibited a thicker pericarp,undeveloped ovules,and few jelly tissues,leading to smaller locules with empty cavities.Histological investigation showed that GA treatment produced more cell layers with larger cells in the pericarp,suggesting its promotion in both cell division and expansion.Transcriptomic analyses between GA-3and mock-treated unpollinated ovaries/fruits identified a large number of differentially expressed genes related to hormones,cell division,cell expansion,and transcription factors,implying that they coordinately regulated parthenocarpy conferred by GA.In particular,the downregulation of five reported repressors of tomato parthenocarpy,including two auxin signaling components,AUXIN RESPONSE FACTOR5(SlARF5)and Sl ARF7,and three MADS-box genes,TOMATO APETALA3(TAP3),TOMATO PISTILLATA(TPI),and AGAMOUS-LIKE6(SlAGL6),after GA treatment might play a key role in this process.Furthermore,we found that the knockdown of a GA signaling factor SlMYB33,which was depressed by GA treatment,induced parthenocarpic fruit set in tomato,an effect that might have been achieved by enhancing GA biosynthesis and decreasing the expression of some repressors of tomato parthenocarpy.Thus,our results provide a basis for understanding the regulatory mechanism of GA in tomato parthenocarpy.
基金supported by the National Natural Science Foundation of China,No.81772421(to YH).
文摘Distraction spinal cord injury is caused by some degree of distraction or longitudinal tension on the spinal cord and commonly occurs in patients who undergo corrective operation for severe spinal deformity.With the increased degree and duration of distraction,spinal cord injuries become more serious in terms of their neurophysiology,histology,and behavior.Very few studies have been published on the specific characteristics of distraction spinal cord injury.In this study,we systematically review 22 related studies involving animal models of distraction spinal cord injury,focusing particularly on the neurophysiological,histological,and behavioral characteristics of this disease.In addition,we summarize the mechanisms underlying primary and secondary injuries caused by distraction spinal cord injury and clarify the effects of different degrees and durations of distraction on the primary injuries associated with spinal cord injury.We provide new concepts for the establishment of a model of distraction spinal cord injury and related basic research,and provide reference guidelines for the clinical diagnosis and treatment of this disease.
文摘Introduction: The frequent late-stage diagnosis of chronic kidney disease (CKD) constrains the treatment choices for nephrologists. Renal biopsy (RB) is crucial for accurately identifying renal lesions. This key nephrological procedure, however, is invasive and not without potential complications. The purpose of this study was to evaluate the indications, frequency, and histological lesion profiles of renal biopsies in Togo. Materials and Methods: We conducted a descriptive cross-sectional study encompassing all renal biopsies performed in Togo from the inception of nephrology services to the present. Data were compiled from the medical records of the patients. Results: From 2015 to 2023, 68 high-quality renal biopsies were executed in Togo. The patients had an average age of 30.6 years, with a predominance of males (69.1%). The most common indication was nephrotic syndrome, accounting for 66.2% of cases. Histologically, glomerulopathies were predominant, representing 61.8% of lesions, followed by vascular nephropathies (25%) and tubulointerstitial nephropathies (13.2%). The most frequently observed primary glomerulopathy was focal segmental glomerulosclerosis (FSGS). Gross hematuria was the sole complication, occurring in 1.4% of the cases. Conclusion: RB is an evolving practice in Togo. Glomerulopathies are the most commonly observed lesions. The histological categorization of renal lesions is vital for clinicians in their diagnostic reasoning and approach.
基金supported by the National Key R&D Program of China (No.2016YFC0901302)。
文摘Objective: To investigate histo-pathological distribution and clinico-pathological significance in a large Chinese triple-negative breast cancer(TNBC) patients serials based on the latest understanding of its clinico-pathological diversity, and to provide more information to clinicians to improve precision of individualized treatment of TNBC.Methods: A retrospective analysis was performed on patients with TNBC at Breast Disease Center, Peking University First Hospital between January 2010 and December 2019. Histo-and clinico-pathological characteristics were analyzed by Chi-square test and Student's t-test, and prognoses were calculated using KaplanMeier method and a Cox proportionate hazards model. Bonferroni correction was used to correct for multiple comparison.Results: Conventional type of TNBC(c TNBC) were identified in 73.7% of 582 TNBC, while special type of TNBC(s TNBC) were 26.3%, including 71 apocrine carcinoma, 20 medullary carcinoma, 31 metaplastic carcinoma, 18 invasive lobular carcinoma, 7 invasive micropapillary carcinoma, 5 adenoid cystic carcinoma and 1 acinic cell carcinoma. Compared to s TNBC, c TNBC was associated with high histologic grade(P<0.001) and lower androgen receptor(AR) expression(P<0.001). TNM stage of low-grade c TNBC was significantly lower than that of high-grade c TNBC(P=0.002). Although no significant difference, there was a trend that the rate of 5-year disease-free survival(DFS) and 5-year overall survival(OS) were longer in high-grade c TNBC than in high-grade s TNBC(P=0.091 and 0.518), and were longer in low-grade s TNBC than in high-grade s TNBC(P=0.051 and0.350). Metaplastic carcinomas showed larger tumor size(P=0.008) and higher proliferative Ki67 index(P=0.004)than c TNBCs.Conclusions: Results from our cohort imply that sub-categorization or subtyping and histological grading could be meaningful in pathological evaluation of TNBC, and need to be clarified in more large collections of TNBC.
基金supported by a grant from the National Key Technology Research and Development Program of China (No.2006AA02A403)
文摘Response criteria remain controversial in therapeutic evaluation for locally advanced esophageal carcinoma treated with neoadjuvant chemotherapy.We aimed to identify the predictive value of tumor regression grading(TRG) in tumor response and prognosis.Fifty-two patients who underwent neoadjuvant chemotherapy followed by esophagectomy and radical 2-field lymphadenectomy between June 2007 and June 2011 were included in this study.All tissue specimens were reassessed according to the TRG scale.Potential prognostic factors,including clinicopathologic factors,were evaluated.Survival curves were generated by using the Kaplan-Meier method and compared with the log-rank test.Prognostic factors were determined with multivariate analysis by using the Cox regression model.Our results showed that of 52 cases,43(83%) were squamous cell carcinoma and 9(17%) were adenocarcinoma.TRG was correlated with pathologic T(P = 0.006) and N(P < 0.001) categories.Median overall survival for the entire cohort was 33 months.The 1-and 2-year overall survival rates were 71% and 44%,respectively.Univariate survival analysis results showed that favorable prognostic factors were histological subtype(P = 0.003),pathologic T category(P = 0.026),pathologic N category(P < 0.001),and TRG G0(P = 0.041).Multivariate analyses identified pathologic N category(P < 0.001) as a significant independent prognostic parameter.Our results indicate that histomorphologic TRG can be considered as an alternative option to predict the therapeutic efficacy and prognostic factor for patients with locally advanced esophageal carcinoma treated by neoadjuvant chemotherapy.
基金supported by the National Key Research and Development Program of China(No.2019YFD1002004).
文摘The environment significantly impacts the interaction between plants and pathogens,thus remarkably affecting crop disease occurrence.However,the detailed combined mechanisms of temperature and humidity influencing this interaction remain unclear.In this study,the interaction between tomato and Botrytis cinerea in various temperature and humidity conditions was analyzed by histological observation and a dual RNA-seq approach.Results showed that low humidity was not favorable for mycelial growth,resulting in infection failure.Both high and low temperatures at high humidity successfully inhibited pathogenic infection and disease incidence in the tomato plants,thus enhancing their resistance to B.cinerea.The high temperature and high humidity(HH)treatment induced the upregulation of light reaction genes,increased the net photosynthetic rate,and expanded the chloroplast morphology of infected tomatoes.The HH treatment also inhibited the expression of cell cycle-related genes of B.cinerea,interfered with conidial germination andmycelial growth,and damagedmycelial cell structure.Lowtemperature and high humidity(LH)treatment induced the expression of cell wall modification genes and remodeled the cell wall morphology of tomatoes in response to B.cinerea.In addition,the downregulated fungal catabolic genes and the abnormal increase in electron density ofmycelial cells under LH treatment subsequently reduced the infection ability of B.cinerea.These results further explain the coupled effects of temperature and humidity on plant defenses and pathogen virulence,and provide a potential means to control gray mold.
基金The study was approved by the Sydney Local Health District Human Research Ethics Committee(HREC CH62/6/2021-055).
文摘BACKGROUND Histological remission is increasingly accepted as a treatment endpoint in the management of ulcerative colitis(UC).However,the knowledge of histology guidelines and the attitudes towards their use in clinical practice by gastroenterologists and pathologists is unknown.AIM To evaluate the knowledge of histology guidelines and attitudes towards the use of histology in UC by gastroenterologists and pathologists.METHODS A prospective,cross-sectional nationwide survey of gastroenterologists and pathologists who analyse UC specimens was conducted.The survey consisted of 34 questions to assess gastroenterologists’and pathologists’knowledge(score out of 19)and attitudes towards histological assessment in UC.Survey questions were formulated using the European Crohn’s and Colitis position paper on histopathology and the British Society of Gastroenterology biopsy reporting guidelines.It included knowledge of histological assessment of disease activity and dysplasia,knowledge of histological scoring systems for ulcerative colitis,uptake of histology scoring systems in routine practice,attitudes towards the role of histological activity,and the use of histological activity in clinical scenarios.RESULTS Of 89 responders(77 gastroenterologists,12 pathologists),there was almost universal acceptance that histological assessment should form part of UC evaluation[95%gastroenterologists,92%pathologists].However,gastroenterologists reported that 92%of their pathologists do not use a histological scoring system.Utilisation of a formal histological scoring system was preferred by 77%of gastroenterologists and 58%of pathologists.Both groups lacked awareness of the Geboes Score,Nancy Index and Robarts Histopathological Index scoring systems with 91%,87%,and 92%of gastroenterologists respectively;and 83%,83%,and 92%pathologists respectively,being uncertain of scoring systems’remission definitions.Histology knowledge score was not significantly different between gastroenterologists and pathologists[9/19(IQR:8-11)vs 8/19(IQR:7-10),P=0.54].Higher knowledge scores were predicted by hospital attending gastroenterologists(P=0.004),participation in inflammatory bowel disease(IBD)multidisciplinary teams(P=0.009),and self-declared IBD sub-specialist(P=0.03).CONCLUSION Histological remission is a recognised target for both gastroenterologists and pathologists.Despite this,knowledge of histological scoring systems and their utilisation is poor.
基金supported by the National Key R&D Program(No.2018YFD0900301-03)the MNR Key Laboratory of Marine Eco-Environmental Science and Technology,China(No.MEEST-2021-04).
文摘Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particularly the benthic organisms.How-ever,the toxic effects of PFOA on the half-smooth tongue sole Cynoglossus semilaevis,a commercial benthic fish in China,have rarely been reported.Because juvenile fish are sensitive to environmental pollutants,in the present study,histological assessment and tran-scriptome sequencing were performed to determine the short-term impact of PFOA on juvenile half-smooth tongue soles.Histologi-cal analysis showed that PFOA exposure caused hepatocyte rupture,intestinal villi breakage,increased goblet cell count,and brain ab-normal.Transcriptome results showed that some interesting signaling pathways,such as glycolysis/gluconeogenesis,PPAR signaling pathway and GABAergic synapse signaling pathway,were enriched after PFOA exposure.In addition,some metabolic,immune and neural genes were differentially expressed,which including ependymin,hbb1-like and gad 1,and they were up-regulated after 14 days of exposure.Transcriptome results also indicated that half-smooth tongue sole might improve energy metabolism in response to PFOA toxicity after 7 days of exposure.These findings provide a basis for studying the ecological effects of PFOA on marine benthic fishes.
基金National Key R&D Program of China(2021YFA1501203)is acknowledged for financial support.
文摘This paper reports the application of multi-component hydrocracking catalyst grading technology in diesel hydrocracking system to increase naphtha,and studies the influence of catalyst systems with different number of graded beds on the reaction process of diesel hydrocracking.Three hydrocracking catalysts with different physicochemical properties as gradation components,the diesel hydrocracking reaction on catalyst systems of one-component,two-component and three-component graded beds with different loading sequences are carried out and evaluated,respectively.The catalytic mechanism of the multi-component grading system is analyzed.The results show that,with the increase of the number of grading beds,the space velocity of reaction on each catalyst increases,which can effectively control the overreaction process;along the flow direction of feedstock,the loading sequences of catalysts with acidity decreasing and pore properties increasing can satisfy the demand of different catalytic activity for the conversion of reactant with changing composition to naphtha,which has a guiding role in the conversion of feedstock to target products.Therefore,the conversion of diesel,the selectivity and yield of naphtha all increase significantly on the multi-component catalyst system.The research on the grading technology of multi-component catalysts is of great significance to the promotion and application of catalyst systems in various catalytic fields.
基金funding from the Korea Health Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (HI17C0881)。
文摘Purpose: This study investigated the anatomical and histological characteristics of the rat Eustachian tube(E-tube)and the feasibility of Eustachian tubography in a rat model.Materials and methods: Fifteen male Wistar rats were used in this study, and the bilateral E-tubes of each rat were examined. Ten E-tubes were used for anatomical studies, another ten for histological analysis, and the other ten for Eustachian tubography. Five rats were euthanized and decapitated, and ten E-tubes were dissected to describe the anatomy of the E-tube. Ten E-tube specimens obtained from five other rats were sectioned to investigate Etube histology. Eustachian tubography was performed on the bilateral E-tubes of the other five rats using the trans-tympanic approach.Results: The rat E-tubes consisted of bony and membranous parts. Cartilage and bone tissue covered only the bony part. The E-tubes’ mean diameter and overall length were 2.97 mm and 4.96 mm, respectively. The tympanic orifices’ mean diameter was 1.21 mm. The epithelium of E-tubes was mainly composed of pseudostratified ciliated and goblet cells. Eustachian tubography was successfully performed on both sides of the E-tube for each rat.The technical success rate was 100%, the average running time was 4.9 min, and no procedure-related complications occurred. On tubography images, the E-tube, tympanic cavity, and nasopharynx could be identified because of the visualization of bony landmarks.Conclusion: In this study, we described the anatomical and histological features of rat E-tubes. With the aid of these findings, E-tube angiography was successfully performed using a transtympanic approach. These results will facilitate further investigation of E-tube dysfunction.
文摘Stains are applied to impart contrast to the tissue and identify particular features of interest. However, the use of synthetic dyes as staining reagents has been associated with significant human health challenges and pollution of the ecosystem. These developments have necessitated a shift towards using natural dyes that are eco-friendlier and readily available. We investigated the staining reaction patterns of teak tree leaves (Tectona grandis) dye extracts and explored their suitability as a cytoplasmic stain in micromorphological assessments. Dye extracts were prepared using acetone, methanol, and ethanol as solvents from air-dried (under shade) teak tree young leaves. The dye extracts were applied as a counterstain and evaluated against eosin in formalin-fixed paraffin-embedded (FFPE) bovine tissue sections at varying concentrations and different staining times. Teak tree leaves (Tectona grandis) dye extracts produced relatively varying staining intensities of reddish-brown cytoplasmic coloration when used on bovine tissue at different concentrations and staining times comparable to eosin and with blue-purple hematoxylin nuclear stain. The present study showed that Tectona grandis leaf dye extracts provide an excellent cytoplasmic staining pattern and can be used as an alternative counterstain in routine H&E staining techniques.
文摘BACKGROUND In Japan,the transhiatal approach,including lower mediastinal lymph node dissection,is widely performed for Siewert type II esophagogastric junction adenocarcinoma.This procedure is generally performed in a magnified view using laparoscopy or a robotic system,therefore,the microanatomy of the lower mediastinum is important.However,mediastinal microanatomy is still unclear and classification of lower mediastinal lymph nodes is not currently based on fascia or other microanatomical structures.AIM To clarify the fascia and layer structures of the lower mediastinum and classify the lower mediastinal tissue.METHODS We dissected the esophagus and surrounding organs en-bloc from seven cadavers fixed in 10%formalin.Organs and tissues were then cut at the level of the lower thoracic esophagus,embedded in paraffin,and serially sectioned.Tissue sections were stained with Hematoxylin-Eosin(all cadavers)and immunostained for the lymphatic endothelial marker D2-40(three cadavers).We observed the periesophageal fasciae and layers,and defined lymph node boundaries based on the fasciae.Lymphatic vessels around the esophagus were observed on immunostained tissue sections.RESULTS We identified two fasciae,A and B.We then classified lower mediastinal tissue into three areas,paraesophageal,paraaortic,and intermediate,using these fasciae as boundaries.Lymph nodes were found to be present and were counted in each area.The dorsal part of the intermediate area was thicker on the caudal side than on the cranial side in all cadavers.On the dorsal side,no blood vessels penetrated the fasciae in six of the seven cadavers,whereas the proper esophageal artery penetrated fascia B in one cadaver.D2-40 immunostaining showed lymphatic vessel connections between the paraesophageal and intermediate areas on the lateral and ventral sides of the esophagus,but no lymphatic connection between areas on the dorsal side of the esophagus.CONCLUSION Histological studies identified two fasciae surrounding the esophagus in the lower mediastinum and the layers separated by these fasciae were used to classify the lower mediastinal tissues.
文摘Diabetes problems can lead to an eye disease called Diabetic Retinopathy(DR),which permanently damages the blood vessels in the retina.If not treated early,DR becomes a significant reason for blindness.To identify the DR and determine the stages,medical tests are very labor-intensive,expensive,and timeconsuming.To address the issue,a hybrid deep and machine learning techniquebased autonomous diagnostic system is provided in this paper.Our proposal is based on lesion segmentation of the fundus images based on the LuNet network.Then a Refined Attention Pyramid Network(RAPNet)is used for extracting global and local features.To increase the performance of the classifier,the unique features are selected from the extracted feature set using Aquila Optimizer(AO)algorithm.Finally,the LightGBM model is applied to classify the input image based on the severity.Several investigations have been done to analyze the performance of the proposed framework on three publically available datasets(MESSIDOR,APTOS,and IDRiD)using several performance metrics such as accuracy,precision,recall,and f1-score.The proposed classifier achieves 99.29%,99.35%,and 99.31%accuracy for these three datasets respectively.The outcomes of the experiments demonstrate that the suggested technique is effective for disease identification and reliable DR grading.
基金supported in part by the Science and Technology Development Plan Project of Changchun[Grant Number 21ZGN28]the Jilin Provincial Science and Technology Development Plan Project[Grant Number 20210101157JC]the Jilin Provincial Science and Technology Development Plan Project[Grant Number 20230202035NC].
文摘To solve inefficient water stress classification of spinach seedlings under complex background,this study proposed an automatic classification method for the water stress level of spinach seedlings based on the N-MobileNetXt(NCAM+MobileNetXt)network.Firstly,this study recon-structed the Sandglass Block to effectively increase the model accuracy;secondly,this study introduced the group convolution module and a two-dimensional adaptive average pool,which can significantly compress the model parameters and enhance the model robustness separately;finally,this study innovatively proposed the Normalization-based Channel Attention Module(NCAM)to enhance the image features obviously.The experimental results showed that the classification accuracy of N-MobileNetXt model for spinach seedlings under the natural environment reached 90.35%,and the number of parameters was decreased by 66%compared with the original MobileNetXt model.The N-MobileNetXt model was superior to other net-work models such as ShuffleNet and GhostNet in terms of parameters and accuracy of identification.It can provide a theoretical basis and technical support for automatic irrigation.
基金the National Natural Science Foundation of China(No.62276210,82201148,61775180)the Natural Science Basic Research Program of Shaanxi Province(No.2022JM-380)+3 种基金the Shaanxi Province College Students'Innovation and Entrepreneurship Training Program(No.S202311664128X)the Natural Science Foundation of Zhejiang Province(No.LQ22H120002)the Medical Health Science and Technology Project of Zhejiang Province(No.2022RC069,2023KY1140)the Natural Science Foundation of Ningbo(No.2023J390)。
文摘Cataract is the leading cause of visual impairment globally.The scarcity and uneven distribution of ophthalmologists seriously hinder early visual impairment grading for cataract patients in the clin-ic.In this study,a deep learning-based automated grading system of visual impairment in cataract patients is proposed using a multi-scale efficient channel attention convolutional neural network(MECA_CNN).First,the efficient channel attention mechanism is applied in the MECA_CNN to extract multi-scale features of fundus images,which can effectively focus on lesion-related regions.Then,the asymmetric convolutional modules are embedded in the residual unit to reduce the infor-mation loss of fine-grained features in fundus images.In addition,the asymmetric loss function is applied to address the problem of a higher false-negative rate and weak generalization ability caused by the imbalanced dataset.A total of 7299 fundus images derived from two clinical centers are em-ployed to develop and evaluate the MECA_CNN for identifying mild visual impairment caused by cataract(MVICC),moderate to severe visual impairment caused by cataract(MSVICC),and nor-mal sample.The experimental results demonstrate that the MECA_CNN provides clinically meaning-ful performance for visual impairment grading in the internal test dataset:MVICC(accuracy,sensi-tivity,and specificity;91.3%,89.9%,and 92%),MSVICC(93.2%,78.5%,and 96.7%),and normal sample(98.1%,98.0%,and 98.1%).The comparable performance in the external test dataset is achieved,further verifying the effectiveness and generalizability of the MECA_CNN model.This study provides a deep learning-based practical system for the automated grading of visu-al impairment in cataract patients,facilitating the formulation of treatment strategies in a timely man-ner and improving patients’vision prognosis.
基金supported by the Beijing Science Foundation(No.9232005)the Beijing Municipal Philosophy and Social Science Foundation of China(No.19GLB036)the Beijing Science and Technology Project(No.Z221100005822014)。
文摘The increasing trend towards independent fruit packaging demands a high appearance quality of individually packed fruits.In this paper,we propose an improved YOLOv5-based model,YOLO-Banana,to effectively grade banana appearance quality based on the number of banana defect points.Due to the minor and dense defects on the surface of bananas,existing detection algorithms have poor detection results and high missing rates.To address this,we propose a densitybased spatial clustering of applications with noise(DBSCAN)and K-means fusion clustering method that utilizes refined anchor points to obtain better initial anchor values,thereby enhancing the network’s recognition accuracy.Moreover,the optimized progressive aggregated network(PANet)enables better multi-level feature fusion.Additionally,the non-maximum suppression function is replaced with a weighted non-maximum suppression(weighted NMS)function based on distance intersection over union(DIoU).Experimental results show that the model’s accuracy is improved by 2.3%compared to the original YOLOv5 network model,thereby effectively grading the banana appearance quality.
