AIM: To study the histopathological changes in the retina and flash electroretinogram(F-ERG) features of ozone-treated streptozotocin(STZ)-induced diabetic rats.METHODS: Seventy male Sprague Dawley rats were grouped a...AIM: To study the histopathological changes in the retina and flash electroretinogram(F-ERG) features of ozone-treated streptozotocin(STZ)-induced diabetic rats.METHODS: Seventy male Sprague Dawley rats were grouped as follows: blank group(GB, n =10), model control group(GM, n =18), ozone group(GO3, n =19), and oxygen group(GO2, n =18). The model was induced by single intraperitoneal injection of STZ. Ozone or oxygen enteroclysm was given twice per week for 4wk. F-ERG and histopathological examinations were performed one month after treatment.RESULTS: Under dark adaption, as compared to GB,the other groups each had differential decreases in the a-wave amplitudes(P <0.05); the latencies were delayed in GM, GO2, and GO3rats(P <0.05). Similar results were observed under light adaption, with the exception that the a-wave of the amplitudes(F =0.28, P >0.05). There were significant differences in the apoptosis index among the groups(P <0.05). Under ozone treatment,apoptosis was decreased in GO3 as compared to GM and GO2.CONCLUSION: Ozone administration alleviates nerve damage and reduces pathology and apoptosis in the retinas of diabetic rats.展开更多
Objective: To explore the genotoxic potential and histopathological changes induced in liver, kidney, testis, brain and heart after using the antibiotic drug amoxicillin/clavulanic acid(4:1).Methods: The study include...Objective: To explore the genotoxic potential and histopathological changes induced in liver, kidney, testis, brain and heart after using the antibiotic drug amoxicillin/clavulanic acid(4:1).Methods: The study included chromosomal aberration analysis in bone-marrow and mouse spermatocytes, induction of sperm morphological abnormalities and histopathological changes in different body organs. The drug was administrated orally at a dose of81 mg/kg body weight twice daily(Total = 162 mg/kg/day) for various periods of time equivalent to 625 mg/men(twice daily).Results: The results revealed non-significant chromosomal aberrations induced after treatment with amoxicillin/clavulanic acid(AC) in both bone marrow and mouse spermatocytes after 7 and 10 days treatment. On the other hand, statistically significant percentages of sperm morphological abnormalities were recorded. Such percentage reached 8.10 ± 0.55, 9.86 ± 0.63 and 12.12 ± 0.58 at the three time intervals tested(7, 14 and 35 days after the 1 st treatment respectively)(treatment performed for 5 successive days) compared with 2.78 ± 0.48 for the control. The results also revealed histopathological changes in different body organs after AC treatment which increased with the prolongation of the period of therapy. Congestion of central vain, liver hemorrhage and hydropic changes in hepatocytes were noticed in the liver. Degenerative changes were found in kidney glomerulus and tubules while testis showed atrophy of seminiferous tubules, and reduction of spermatogenesis. AC also induced neurotoxicity and altered brain neurotransmitter levels. Hemorrhage in the myocardium, disruption of cardiac muscle fibers and pyknotic nuclei in cardiomyocytes were recorded as side effects of AC in heart tissue.Conclusions: The results concluded that AC treatment induced sperm morphological abnormalities and histopathological changes in different body organs. Clinicians must be aware of such results while describing the drug.展开更多
To study protective effects of Qingshen Compound on experimental anti-glomerular basement membrane antibody (AGBM-Ab) nephritis model in rats,an experimental Masugi nephritis model was established in Sprague-Dawley(SD...To study protective effects of Qingshen Compound on experimental anti-glomerular basement membrane antibody (AGBM-Ab) nephritis model in rats,an experimental Masugi nephritis model was established in Sprague-Dawley(SD) rats by a single-dose injection of prepared rabbit anti-rat glomerular basement membrane(GBM) serum into sublingual vein and then effects of Qingshen Compound on urine volume,urinary protein level,blood urea nitrogen (BUN),creatinine (Cr),albumin (Alb),triglycerides (TG),cholesterol (CHO) and histopathological changes were investigated.After successive daily three-dosage oral administration to rat groups (9.4,4.7,2.3 g·kg-1) for 8 weeks,Qingshen Compound was proved effective on increasing urine volume and Alb content,and decreasing levels of urinary protein,BUN,Cr and TG.Furthermore,high and middle dosages (9.4,4.7 g·kg-1) could improve histopathological healing of the model.In summary,Qingshen Compound has shown protective effects on experimental Masugi nephritis model in rats.展开更多
Coronavirus disease 2019(COVID-19)may rapidly worsen respiratory failure,thereby leading to death.COVID-19-induced respiratory failure exhibits some atypical characteristics,silent hypoxemia,and high lung compliance.S...Coronavirus disease 2019(COVID-19)may rapidly worsen respiratory failure,thereby leading to death.COVID-19-induced respiratory failure exhibits some atypical characteristics,silent hypoxemia,and high lung compliance.Some histopathological changes associated with COVID-19-induced respiratory failure differ from those of classic acute respiratory distress syndrome(ARDS).However,compared with classical ARDS,COVID-19-induced respi-ratory failure has a similar timing of onset,clinical syndromes,radiological profile,and mortality rate in the intensive care unit(ICU).Respiratory failure induced by COVID-19 is a type of ARDS and is currently underdiag-nosed.This condition stretches the definition of classic ARDS;therefore,an updated definition is warranted.展开更多
基金Supported by the Xinjiang Natural Science Research Fund (No. 2014211C046)
文摘AIM: To study the histopathological changes in the retina and flash electroretinogram(F-ERG) features of ozone-treated streptozotocin(STZ)-induced diabetic rats.METHODS: Seventy male Sprague Dawley rats were grouped as follows: blank group(GB, n =10), model control group(GM, n =18), ozone group(GO3, n =19), and oxygen group(GO2, n =18). The model was induced by single intraperitoneal injection of STZ. Ozone or oxygen enteroclysm was given twice per week for 4wk. F-ERG and histopathological examinations were performed one month after treatment.RESULTS: Under dark adaption, as compared to GB,the other groups each had differential decreases in the a-wave amplitudes(P <0.05); the latencies were delayed in GM, GO2, and GO3rats(P <0.05). Similar results were observed under light adaption, with the exception that the a-wave of the amplitudes(F =0.28, P >0.05). There were significant differences in the apoptosis index among the groups(P <0.05). Under ozone treatment,apoptosis was decreased in GO3 as compared to GM and GO2.CONCLUSION: Ozone administration alleviates nerve damage and reduces pathology and apoptosis in the retinas of diabetic rats.
基金supported by the project in National Research Centre under grant number: 10090013, Cairo, Egypt
文摘Objective: To explore the genotoxic potential and histopathological changes induced in liver, kidney, testis, brain and heart after using the antibiotic drug amoxicillin/clavulanic acid(4:1).Methods: The study included chromosomal aberration analysis in bone-marrow and mouse spermatocytes, induction of sperm morphological abnormalities and histopathological changes in different body organs. The drug was administrated orally at a dose of81 mg/kg body weight twice daily(Total = 162 mg/kg/day) for various periods of time equivalent to 625 mg/men(twice daily).Results: The results revealed non-significant chromosomal aberrations induced after treatment with amoxicillin/clavulanic acid(AC) in both bone marrow and mouse spermatocytes after 7 and 10 days treatment. On the other hand, statistically significant percentages of sperm morphological abnormalities were recorded. Such percentage reached 8.10 ± 0.55, 9.86 ± 0.63 and 12.12 ± 0.58 at the three time intervals tested(7, 14 and 35 days after the 1 st treatment respectively)(treatment performed for 5 successive days) compared with 2.78 ± 0.48 for the control. The results also revealed histopathological changes in different body organs after AC treatment which increased with the prolongation of the period of therapy. Congestion of central vain, liver hemorrhage and hydropic changes in hepatocytes were noticed in the liver. Degenerative changes were found in kidney glomerulus and tubules while testis showed atrophy of seminiferous tubules, and reduction of spermatogenesis. AC also induced neurotoxicity and altered brain neurotransmitter levels. Hemorrhage in the myocardium, disruption of cardiac muscle fibers and pyknotic nuclei in cardiomyocytes were recorded as side effects of AC in heart tissue.Conclusions: The results concluded that AC treatment induced sperm morphological abnormalities and histopathological changes in different body organs. Clinicians must be aware of such results while describing the drug.
文摘To study protective effects of Qingshen Compound on experimental anti-glomerular basement membrane antibody (AGBM-Ab) nephritis model in rats,an experimental Masugi nephritis model was established in Sprague-Dawley(SD) rats by a single-dose injection of prepared rabbit anti-rat glomerular basement membrane(GBM) serum into sublingual vein and then effects of Qingshen Compound on urine volume,urinary protein level,blood urea nitrogen (BUN),creatinine (Cr),albumin (Alb),triglycerides (TG),cholesterol (CHO) and histopathological changes were investigated.After successive daily three-dosage oral administration to rat groups (9.4,4.7,2.3 g·kg-1) for 8 weeks,Qingshen Compound was proved effective on increasing urine volume and Alb content,and decreasing levels of urinary protein,BUN,Cr and TG.Furthermore,high and middle dosages (9.4,4.7 g·kg-1) could improve histopathological healing of the model.In summary,Qingshen Compound has shown protective effects on experimental Masugi nephritis model in rats.
基金the National Science and Technology Major Project for Control and Prevention of Major Infectious Diseases of China(Grant Number:2017ZX10103004)the National Natural Science Foundation of China(Grant Numbers:81671892,81971888)+3 种基金the Jiangsu Province’s Key Discipline/Laboratory of Medicine(Grant Number:ZDXKA2016025)the Jiangsu Province’s Key Provincial Talents Program(Grant Number:ZDRCA2016082)the Natural Science Foundation of Jiangsu Province(Grant Number:BK20161433)and the Postgraduate Research and Practice Innovation Program of Jiangsu Province(Grant Number:KYCX180181).
文摘Coronavirus disease 2019(COVID-19)may rapidly worsen respiratory failure,thereby leading to death.COVID-19-induced respiratory failure exhibits some atypical characteristics,silent hypoxemia,and high lung compliance.Some histopathological changes associated with COVID-19-induced respiratory failure differ from those of classic acute respiratory distress syndrome(ARDS).However,compared with classical ARDS,COVID-19-induced respi-ratory failure has a similar timing of onset,clinical syndromes,radiological profile,and mortality rate in the intensive care unit(ICU).Respiratory failure induced by COVID-19 is a type of ARDS and is currently underdiag-nosed.This condition stretches the definition of classic ARDS;therefore,an updated definition is warranted.
