Rationale:Guillain Barre syndrome(GBS)is an acute neurological illness leading to quadriparesis with respiratory involvement.It can be triggered by infections,vaccinations,surgery,trauma,transplantation and drugs.Anti...Rationale:Guillain Barre syndrome(GBS)is an acute neurological illness leading to quadriparesis with respiratory involvement.It can be triggered by infections,vaccinations,surgery,trauma,transplantation and drugs.Anti-rabies cell culture vaccines introduced to overcome the high rate of neurological complications associated with tissue based rabies vaccine,can be very rarely associated with GBS.Patient concerns:A 50-year-old female presented with acute severe upper back pain evolving into pure motor quadriparesis following administration of human diploid cell vaccine for rabies.Diagnosis:Acute motor axonal neuropathy variant of GBS following anti-rabies human diploid cell vaccine.Interventions:Intravenous high dose steroids.Outcomes:Patient recovered completely within 1 month.Lessons:Although anti-rabies cell culture vaccines are highly immunogenic and safe,they are rarely associated with GBS.Clinicians should be aware of this link because prompt diagnosis and treatment can result in complete recovery and avoid complications.展开更多
A series of bioassays such as sister chromatid exchange frequencies ( SCE.), chromosomal aberration ( CA ), micronuclel rate (MN) and cell-cycle delay have been used to detecting the genotoxic effect of cigarette smok...A series of bioassays such as sister chromatid exchange frequencies ( SCE.), chromosomal aberration ( CA ), micronuclel rate (MN) and cell-cycle delay have been used to detecting the genotoxic effect of cigarette smoke condensate (CSC) on human diploid cell 2BS strain. The results suggested that a higher SCE, ( 17. 0/ cell) was observed In 2BS cells treated with CSC at 100 μg/ml, as compared with 6. 9/cell of the background (P<0. 001). CA rate was significantly increased from 4% to 36% In cells treated with 10 μg/ml CSC (P< 0.001). MN rate varied from 9 -26‰ In cells treated with CSC compared to that of control (6‰). Meanwhile, the cell-cycle of cells was markedly delayed by CSC. The survival rate of 2BS cells declined to 59. 6% for treatment with CSC at 200 μg/ ml. There was a dose-effect response In SCE., CA, MN rate. We proposed that active oxygen might responsible for genotoxiclty of CSC on cells.展开更多
目的探讨沉默信息调节因子3(SIRT3)在高糖引起的人二倍体成纤维细胞(WI-38)衰老过程中的表达变化和意义。方法通过低糖(3.34mmol/L)、正常糖(5.56mmol/L)、高糖(25mmol/L)三种不同条件培养WI-38细胞,使之由20群体倍增水平(PDs)增至32PD...目的探讨沉默信息调节因子3(SIRT3)在高糖引起的人二倍体成纤维细胞(WI-38)衰老过程中的表达变化和意义。方法通过低糖(3.34mmol/L)、正常糖(5.56mmol/L)、高糖(25mmol/L)三种不同条件培养WI-38细胞,使之由20群体倍增水平(PDs)增至32PDs。采用Western blotting检测p21、p27、Catalase、SIRT3和MnSOD的蛋白表达;免疫荧光共染方法检测各组WI-38细胞中SIRT3和MnSOD和衰老相关核异染色质灶(SAHF)的表达和定位;用荧光探针检测活性氧(ROS)的表达。结果 Western blot-ting结果显示,高糖组SIRT3、Catalase和MnSOD的表达与低糖组及正常糖组比较明显降低(P<0.05);高糖组的p21p、27的蛋白表达与正常糖组比较明显增加(P<0.05);与低糖组比较,高糖组的p21表达上调,而p27表达差异无统计学意义(P>0.05)。免疫荧光共染结果显示,高糖组中的SIRT3、Catalase和MnSOD表达较其他两组明显降低(P<0.05);SIRT3和MnSOD主要存在于胞质中。荧光探针显示,高糖组中的ROS水平与低糖组和正常糖组比较明显增加。结论高糖能加速WI-38细胞的衰老过程,SIRT3可能与高糖引起的细胞衰老有着密切联系。展开更多
目的:探讨不同感染复数(Multiplicity of infection,MOI)的狂犬病毒PM株在人二倍体细胞(2BS株)中增殖的影响,确定感染复数。方法将狂犬病毒PM株按照MOI 0.01、0.02、0.05、0.10、0.15、0.20接种至2BS细胞中,用显微镜观察接种病毒...目的:探讨不同感染复数(Multiplicity of infection,MOI)的狂犬病毒PM株在人二倍体细胞(2BS株)中增殖的影响,确定感染复数。方法将狂犬病毒PM株按照MOI 0.01、0.02、0.05、0.10、0.15、0.20接种至2BS细胞中,用显微镜观察接种病毒后细胞的形态变化;培养3-5d后,第一次收获病毒液;更换培养液继续培养3-4d后,进行第二次收获。采用小鼠脑内滴定法测定狂犬病毒滴度,通过NIH法测定灭活病毒液的效价。结果当MOI为0.05-0.10时,细胞圆缩30%-40%,细胞能维持较好的生长形态,可收获病毒,病毒收获液的滴度较高(〉5.0 lgLD50/mL),且灭活后病毒液的效价较高(〉4.0IU/mL)。结论确定狂犬病毒PM株在人二倍体2BS细胞增殖的适宜MOI,为人用狂犬病疫苗生产工艺的优化提供了依据。展开更多
目的研究人二倍体细胞KMB17表面与肠道病毒EV71感染相关的受体Toll样受体4(TLR4)、血小板衍生生长因子受体α多肽(pDGFR-αR1)的表达情况,以及细胞周期、细胞代次、EV71感染对这两种受体表达的影响。方法使用荧光标记的特异抗体:PE-TLR4...目的研究人二倍体细胞KMB17表面与肠道病毒EV71感染相关的受体Toll样受体4(TLR4)、血小板衍生生长因子受体α多肽(pDGFR-αR1)的表达情况,以及细胞周期、细胞代次、EV71感染对这两种受体表达的影响。方法使用荧光标记的特异抗体:PE-TLR4、FITC-PDGFR-αR1标记不同细胞代次、处于不同细胞周期时段的KMB17细胞,应用流式细胞仪检测表达这两种受体蛋白的阳性细胞率,以及EV71感染前后,阳性细胞率的变化。使用NCBI primer blast设计TLR4、PDGFR-αR1特异的引物,提取不同细胞代次、细胞周期时段KMB17细胞的RNA,使用real-timePCR检测这些受体蛋白特异mRNA的量。结果 KMB17细胞在使用血清饥饿法进行G0/G1同步化后约22h进入S期,在36h进入G2期,在48h左右完成分裂。PDGFR-αR1、TLR4平均阳性细胞的比例分别为4.47%和11.82%;通过流式细胞仪检测,在G0/G1期,PDGFR-αR1、TLR4的平均阳性细胞比例分别为4%和8.9%;在S期,分别为2.6%和9%;在G2/M期,分别为4.4%和13.5%;EV71病毒感染后,PDGFR-αR1阳性的细胞比率由2.6%上升至4.0%,TLR4阳性细胞比率分别为11.6%和12.8%。结论人二倍体细胞KMB17表面表达肠道病毒相关的受体TLR4和PDGFR-αR1,表达PDGFR-αR1的阳性细胞较TLR4少,不同代次的KMB17细胞这2种受体阳性细胞比例的差异不大,PDGFR-αR1阳性细胞在G0期较多,TLR4阳性细胞在G2/M期较多;EV71病毒感染后,PDGFR-αR1、TLR4阳性细胞的比率变化不大,表明这两种受体分子在KMB17细胞表面表达较稳定,不受EV71感染的影响。展开更多
目的:研究银杏叶总黄酮(total flavone of Ginkgo biloba,FG)延缓人胚肺二倍体成纤维细胞衰老的作用。方法:采用含FG的大鼠血清对人胚肺二倍体成纤维细胞2BS细胞株进行处理,观察2BS细胞寿命;采用荧光实时定量PCR法检测P16基因mRNA的表...目的:研究银杏叶总黄酮(total flavone of Ginkgo biloba,FG)延缓人胚肺二倍体成纤维细胞衰老的作用。方法:采用含FG的大鼠血清对人胚肺二倍体成纤维细胞2BS细胞株进行处理,观察2BS细胞寿命;采用荧光实时定量PCR法检测P16基因mRNA的表达。结果:银杏叶总黄酮(FG)能够延长2BS细胞的传代寿命,下调2BS细胞P16基因mRNA的表达。结论:银杏叶总黄酮可通过抑制P16基因表达而延缓细胞衰老。展开更多
文摘Rationale:Guillain Barre syndrome(GBS)is an acute neurological illness leading to quadriparesis with respiratory involvement.It can be triggered by infections,vaccinations,surgery,trauma,transplantation and drugs.Anti-rabies cell culture vaccines introduced to overcome the high rate of neurological complications associated with tissue based rabies vaccine,can be very rarely associated with GBS.Patient concerns:A 50-year-old female presented with acute severe upper back pain evolving into pure motor quadriparesis following administration of human diploid cell vaccine for rabies.Diagnosis:Acute motor axonal neuropathy variant of GBS following anti-rabies human diploid cell vaccine.Interventions:Intravenous high dose steroids.Outcomes:Patient recovered completely within 1 month.Lessons:Although anti-rabies cell culture vaccines are highly immunogenic and safe,they are rarely associated with GBS.Clinicians should be aware of this link because prompt diagnosis and treatment can result in complete recovery and avoid complications.
文摘A series of bioassays such as sister chromatid exchange frequencies ( SCE.), chromosomal aberration ( CA ), micronuclel rate (MN) and cell-cycle delay have been used to detecting the genotoxic effect of cigarette smoke condensate (CSC) on human diploid cell 2BS strain. The results suggested that a higher SCE, ( 17. 0/ cell) was observed In 2BS cells treated with CSC at 100 μg/ml, as compared with 6. 9/cell of the background (P<0. 001). CA rate was significantly increased from 4% to 36% In cells treated with 10 μg/ml CSC (P< 0.001). MN rate varied from 9 -26‰ In cells treated with CSC compared to that of control (6‰). Meanwhile, the cell-cycle of cells was markedly delayed by CSC. The survival rate of 2BS cells declined to 59. 6% for treatment with CSC at 200 μg/ ml. There was a dose-effect response In SCE., CA, MN rate. We proposed that active oxygen might responsible for genotoxiclty of CSC on cells.
文摘目的 评价不同细胞基质制备的肠道病毒71型(EV71)灭活疫苗上市后常规免疫安全性。 方法 EV71灭活疫苗疑似预防接种异常反应(AEFI)数据来源于中国免疫规划信息管理系统,为2017年接种EV71 灭活疫苗后江苏省报告的监测数据;受种儿童人口学信息和接种剂次数来源于江苏省预防接种信息管理系统。 结果 2017年江苏省共接种EV71灭活疫苗316 889剂,报告AEFI 208例,报告发生率为65.64/10万剂(95% CI :56.72/10万剂~74.56/10万剂),其中一般反应55.54/10万剂(95% CI :47.34/10万剂~63.74/10万剂),异常反应 7.89/10万剂(95% CI :4.80/10万剂~10.98/10万剂);不同细胞基质的疫苗AEFI发生率(χ 2 =35.61, P <0.01)和一般反应发生率(χ 2 =34.09, P <0.01)差异有统计学意义,异常反应发生率差异无统计学意义(χ 2 =1.88, P =0.17),均未见严重异常反应,208例AEFI均未住院且均痊愈。EV71灭活疫苗(Vero细胞)首剂接种一般反应发生率高于第二剂(χ 2 =6.94, P <0.01),不同季度接种EV71灭活疫苗(人二倍体细胞)一般反应发生率差异有统计学意义(χ 2 =18.86, P <0.01)。 结论 不同细胞基质的EV71灭活疫苗在常规免疫接种中AEFI发生率均不高,有良好的安全性;一般反应以发热为主,异常反应以过敏性-荨麻疹、过敏性皮疹和过敏性斑丘疹等过敏性反应为主。
文摘目的探讨沉默信息调节因子3(SIRT3)在高糖引起的人二倍体成纤维细胞(WI-38)衰老过程中的表达变化和意义。方法通过低糖(3.34mmol/L)、正常糖(5.56mmol/L)、高糖(25mmol/L)三种不同条件培养WI-38细胞,使之由20群体倍增水平(PDs)增至32PDs。采用Western blotting检测p21、p27、Catalase、SIRT3和MnSOD的蛋白表达;免疫荧光共染方法检测各组WI-38细胞中SIRT3和MnSOD和衰老相关核异染色质灶(SAHF)的表达和定位;用荧光探针检测活性氧(ROS)的表达。结果 Western blot-ting结果显示,高糖组SIRT3、Catalase和MnSOD的表达与低糖组及正常糖组比较明显降低(P<0.05);高糖组的p21p、27的蛋白表达与正常糖组比较明显增加(P<0.05);与低糖组比较,高糖组的p21表达上调,而p27表达差异无统计学意义(P>0.05)。免疫荧光共染结果显示,高糖组中的SIRT3、Catalase和MnSOD表达较其他两组明显降低(P<0.05);SIRT3和MnSOD主要存在于胞质中。荧光探针显示,高糖组中的ROS水平与低糖组和正常糖组比较明显增加。结论高糖能加速WI-38细胞的衰老过程,SIRT3可能与高糖引起的细胞衰老有着密切联系。
文摘目的:探讨不同感染复数(Multiplicity of infection,MOI)的狂犬病毒PM株在人二倍体细胞(2BS株)中增殖的影响,确定感染复数。方法将狂犬病毒PM株按照MOI 0.01、0.02、0.05、0.10、0.15、0.20接种至2BS细胞中,用显微镜观察接种病毒后细胞的形态变化;培养3-5d后,第一次收获病毒液;更换培养液继续培养3-4d后,进行第二次收获。采用小鼠脑内滴定法测定狂犬病毒滴度,通过NIH法测定灭活病毒液的效价。结果当MOI为0.05-0.10时,细胞圆缩30%-40%,细胞能维持较好的生长形态,可收获病毒,病毒收获液的滴度较高(〉5.0 lgLD50/mL),且灭活后病毒液的效价较高(〉4.0IU/mL)。结论确定狂犬病毒PM株在人二倍体2BS细胞增殖的适宜MOI,为人用狂犬病疫苗生产工艺的优化提供了依据。
文摘目的研究人二倍体细胞KMB17表面与肠道病毒EV71感染相关的受体Toll样受体4(TLR4)、血小板衍生生长因子受体α多肽(pDGFR-αR1)的表达情况,以及细胞周期、细胞代次、EV71感染对这两种受体表达的影响。方法使用荧光标记的特异抗体:PE-TLR4、FITC-PDGFR-αR1标记不同细胞代次、处于不同细胞周期时段的KMB17细胞,应用流式细胞仪检测表达这两种受体蛋白的阳性细胞率,以及EV71感染前后,阳性细胞率的变化。使用NCBI primer blast设计TLR4、PDGFR-αR1特异的引物,提取不同细胞代次、细胞周期时段KMB17细胞的RNA,使用real-timePCR检测这些受体蛋白特异mRNA的量。结果 KMB17细胞在使用血清饥饿法进行G0/G1同步化后约22h进入S期,在36h进入G2期,在48h左右完成分裂。PDGFR-αR1、TLR4平均阳性细胞的比例分别为4.47%和11.82%;通过流式细胞仪检测,在G0/G1期,PDGFR-αR1、TLR4的平均阳性细胞比例分别为4%和8.9%;在S期,分别为2.6%和9%;在G2/M期,分别为4.4%和13.5%;EV71病毒感染后,PDGFR-αR1阳性的细胞比率由2.6%上升至4.0%,TLR4阳性细胞比率分别为11.6%和12.8%。结论人二倍体细胞KMB17表面表达肠道病毒相关的受体TLR4和PDGFR-αR1,表达PDGFR-αR1的阳性细胞较TLR4少,不同代次的KMB17细胞这2种受体阳性细胞比例的差异不大,PDGFR-αR1阳性细胞在G0期较多,TLR4阳性细胞在G2/M期较多;EV71病毒感染后,PDGFR-αR1、TLR4阳性细胞的比率变化不大,表明这两种受体分子在KMB17细胞表面表达较稳定,不受EV71感染的影响。
文摘目的:研究银杏叶总黄酮(total flavone of Ginkgo biloba,FG)延缓人胚肺二倍体成纤维细胞衰老的作用。方法:采用含FG的大鼠血清对人胚肺二倍体成纤维细胞2BS细胞株进行处理,观察2BS细胞寿命;采用荧光实时定量PCR法检测P16基因mRNA的表达。结果:银杏叶总黄酮(FG)能够延长2BS细胞的传代寿命,下调2BS细胞P16基因mRNA的表达。结论:银杏叶总黄酮可通过抑制P16基因表达而延缓细胞衰老。