hb(hunchback) is a contributing factor in anteroposterior axial patterning of insects.Although the hb function in Locusta migratoria manilensis has been investigated,its expression pattern remains unknown.Here,the mou...hb(hunchback) is a contributing factor in anteroposterior axial patterning of insects.Although the hb function in Locusta migratoria manilensis has been investigated,its expression pattern remains unknown.Here,the mouse polyclonal antibody was produced against Hb fusion protein,and then its expression pattern during oogenesis and embryogenesis of L.migratoria manilensis was examined by immunohistochemical staining.Hb protein was detected in the oocyte nucleus which was positioned centrally within the developing oocyte.The oocyte nucleus gradually moved to the posterior end of the egg along with the oocyte maturing.In freshly laid eggs,Hb formed gradient at the posterior end of the egg,and then hb was expressed as a band in the middle of the blastodisc.As the blastodisc differentiated into the head and trunk,the expression region became wide,which would develop into spatial gnathal and thoracic segments.With abdominal segmentation,the expression domain in the gnathal and thoracic region became faint and eventually faded out,while the Hb expression domain appeared at the posterior growth zone in a discontinuous expression manner.The hb expression pattern of L.migratoria manilensis is greatly similar to that of other locusts,such as Schistocerca americana and another L.migratoria.Compared with other insects,hb expression is conserved in the gnathal and thoracic domains,while divergent in oogenesis and abdomen.展开更多
目的探讨1例伴皮质下梗死和白质脑病的常染色体显性脑动脉病(cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy,CADASIL)患者的临床特征和基因检测结果.方法收集患者及其家系成员的临床表...目的探讨1例伴皮质下梗死和白质脑病的常染色体显性脑动脉病(cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy,CADASIL)患者的临床特征和基因检测结果.方法收集患者及其家系成员的临床表型、神经影像学及基因检测资料,结合文献总结其临床特点.结果患者无头痛、反复卒中、痴呆、情感障碍等CADASIL的典型临床表现,仅表现为进行性加重的帕金森症状以及后期出现的腰痛、驼背、吞咽困难、视物重影等.头颅MRI扫描显示左侧基底节和外囊腔隙性脑梗死.基因检测发现NOTCH3第11外显子c.1630C>T(p.R544C)变异.其母亲、姐姐和弟弟均携带c.1630C>T(p.R544C)变异,但具有不同的表型.结论CADASIL的临床表型有较大的异质性,腰痛、驼背以及帕金森综合征可能是一种少见的临床表型.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 30700435)Program of Natural Science Foundation of Chongqing (Grant No. CSTC2009BB1387)
文摘hb(hunchback) is a contributing factor in anteroposterior axial patterning of insects.Although the hb function in Locusta migratoria manilensis has been investigated,its expression pattern remains unknown.Here,the mouse polyclonal antibody was produced against Hb fusion protein,and then its expression pattern during oogenesis and embryogenesis of L.migratoria manilensis was examined by immunohistochemical staining.Hb protein was detected in the oocyte nucleus which was positioned centrally within the developing oocyte.The oocyte nucleus gradually moved to the posterior end of the egg along with the oocyte maturing.In freshly laid eggs,Hb formed gradient at the posterior end of the egg,and then hb was expressed as a band in the middle of the blastodisc.As the blastodisc differentiated into the head and trunk,the expression region became wide,which would develop into spatial gnathal and thoracic segments.With abdominal segmentation,the expression domain in the gnathal and thoracic region became faint and eventually faded out,while the Hb expression domain appeared at the posterior growth zone in a discontinuous expression manner.The hb expression pattern of L.migratoria manilensis is greatly similar to that of other locusts,such as Schistocerca americana and another L.migratoria.Compared with other insects,hb expression is conserved in the gnathal and thoracic domains,while divergent in oogenesis and abdomen.
文摘目的探讨1例伴皮质下梗死和白质脑病的常染色体显性脑动脉病(cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy,CADASIL)患者的临床特征和基因检测结果.方法收集患者及其家系成员的临床表型、神经影像学及基因检测资料,结合文献总结其临床特点.结果患者无头痛、反复卒中、痴呆、情感障碍等CADASIL的典型临床表现,仅表现为进行性加重的帕金森症状以及后期出现的腰痛、驼背、吞咽困难、视物重影等.头颅MRI扫描显示左侧基底节和外囊腔隙性脑梗死.基因检测发现NOTCH3第11外显子c.1630C>T(p.R544C)变异.其母亲、姐姐和弟弟均携带c.1630C>T(p.R544C)变异,但具有不同的表型.结论CADASIL的临床表型有较大的异质性,腰痛、驼背以及帕金森综合征可能是一种少见的临床表型.
