Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition ...Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition of tumor cells and spheroids were assessed using MTT and 3D culture assays.Quantitative real-time PCR and Western blot analyses were employed to investigate the changes in mRNA and protein expression levels of molecules related to cell cycle and apoptosis.Results:Hydrangea serrata extract effectively inhibited the growth of both tumor cells and spheroids.The extract also significantly upregulated p27 mRNA expression and downregulated CDK2 mRNA expression,leading to cell cycle arrest.Moreover,increased BAX/Bcl-2 ratio as well as caspase-9 and-3 were observed after treatment with Hydrangea serrata extract,indicating the induction of tumor cell apoptosis.Conclusions:Hydrangea serrata extract has the potential to alleviate tumors by effectively modulating cell-cycle-related gene expressions and inducing apoptosis,thereby inhibiting tumor growth.展开更多
为深入研究绣球属植物花粉形态的分类学价值和系统学意义,厘清绣球属与近缘属之间的系统发育关系,该文利用扫描电子显微镜(SEM,scanning electron microscope)对国产绣球属及其近缘属41种绣球花科(Hydrangeaceae)植物的花粉形态以及表...为深入研究绣球属植物花粉形态的分类学价值和系统学意义,厘清绣球属与近缘属之间的系统发育关系,该文利用扫描电子显微镜(SEM,scanning electron microscope)对国产绣球属及其近缘属41种绣球花科(Hydrangeaceae)植物的花粉形态以及表面纹饰进行了观察。结果表明:绣球属及其近缘属的花粉为三孔沟;形状多数为长圆体形或近球体形;赤道面观为椭圆形或圆形;极面观多为圆形,少数为三角形或圆三角形。花粉外壁纹饰可分为网状和孔穴状。网眼内的三级纹饰可分为光滑和具颗粒状突起。根据花粉形状和外壁纹饰类型将上述物种划分为4个组,即花粉的形状为长圆体形,表面纹饰为孔穴纹饰;花粉的形状为长圆体形,表面纹饰为网状纹饰;花粉的形状为近球体形,表面纹饰为孔穴纹饰;花粉的形状为近球体形,表面纹饰为网状纹饰。以上可进一步细分为8个类型。上述表明花粉形态证据可为绣球属及其近缘属的属下分类和种间界定提供重要佐证;但结合前人的系统发育重建分析该属植物花粉形态的系统学意义相对有限,如花粉形态证据对于该属及其近缘种属系统发育树上大支的界定难以提供有力的证据。展开更多
A new kaempferol glycoside,named kaempferol 3-O-[6″-O-β-D-glucopyranosyl-6′′′-O-α-L-rhamnopy ranosyl-2′′′′-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,was isolated from Hydrangea macrophylla(Thunb.) Seringe...A new kaempferol glycoside,named kaempferol 3-O-[6″-O-β-D-glucopyranosyl-6′′′-O-α-L-rhamnopy ranosyl-2′′′′-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,was isolated from Hydrangea macrophylla(Thunb.) Seringe.Its structure was establishedby spectroscopic techniques including MS,IR,UV,and 2D NMR.展开更多
Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-sta...Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions.In this study,nitrogen requirements for producing Hydrangea macrophylla‘Hanatemari’that robust seedlings,nutrient dynamics,biomass and growth,was examined utilizing exponential fertilization.The potted seedlings were fertilized with urea under exponential regime at rates of 0.5,1.5 and 2.0 g nitrogen/plant(EF1,EF2,and EF3),respectively.In addition,an unfertilized group treated with equal volume of deionized water was used as control.The results showed that seedlings under 1.5 g N/plant(EF2)had the highest plant growth index and total biomass.The nutrient concentrations of different organs varied in different fertilization treatments.Based on the results of current study,it is concluded that 1.5 g N/plant(EF2)is suitable exponential fertilization treatment for the culture of hydrangea seedlings.Our treatments results showed that 2.0 g N/plant is not suitable for seedling culturing,because of serious nutrient toxicity.These findings will help to improve seedling quality and strengthen the production of H.macrophylla for plantation.展开更多
Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we establish...Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we established an efficient regeneration system by using stem segments as explants for the first time.In our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root induction.We found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(GA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)IBA.In summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.展开更多
为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Ko...为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Komachi’)、‘佳澄’(‘Kasumi’)、‘无尽夏’(‘Endless Summer’)和不耐寒品种‘甜蜜幻想’(‘Sweet Fantasy’)、‘灵感’(‘Inspiration’)、‘你我的情感’(‘You and Me Feelings’)叶的抗氧化酶活性及丙二醛(MDA)和渗透调节物质含量进行比较,并对这些耐寒指标进行相关性分析和主成分分析。结果表明:供试绣球品种LT 50的波动较大,变化范围为-36.68℃~-1.87℃,其中,‘小町’的LT_(50)最低,‘佳澄’、‘平瓣’(‘Flat’)、‘蒂亚娜’(‘Tijana’)、‘塞尔玛’(‘Selma’)、‘含羞叶’(‘Elbtal’)、‘花手鞠’(‘Stockings’)、‘蓝色多瑙河’(‘Blue Danube’)和‘无尽夏’的LT_(50)均低于-20℃。40个绣球品种的耐寒性被分成不耐寒、较耐寒、耐寒和强耐寒4个等级,分别包含13、18、8和1个品种。6个代表品种的过氧化氢酶(CAT)、多酚氧化酶(PPO)和超氧化物歧化酶活性及MDA、脯氨酸(Pro)、可溶性蛋白质(SP)和可溶性糖含量随温度降低基本呈先升高后降低的变化趋势。相关性分析结果表明PPO活性和Pro含量与LT_(50)呈极显著(P<0.01)负相关。主成分分析结果表明:前4个主成分的累计贡献率为81.212%,说明这4个主成分基本能够反映绣球品种的耐寒性;PPO活性、CAT活性、Pro含量和SP含量的负荷量绝对值分别在第1、第2、第3和第4主成分中最高。研究结果显示:供试绣球品种间的耐寒性差异较大,‘小町’等9个品种的耐寒性较强,能够在冬季温度偏低地区露天栽培,并可作为绣球耐寒品种选育的亲本。PPO活性、CAT活性、Pro含量和SP含量为绣球耐寒性评价的主要指标,尤其是PPO活性和Pro含量。展开更多
基金funded by the GRRC Program of Gyeonggi province[GRRC-KyungHee2023(B01)],Republic of Korea.
文摘Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition of tumor cells and spheroids were assessed using MTT and 3D culture assays.Quantitative real-time PCR and Western blot analyses were employed to investigate the changes in mRNA and protein expression levels of molecules related to cell cycle and apoptosis.Results:Hydrangea serrata extract effectively inhibited the growth of both tumor cells and spheroids.The extract also significantly upregulated p27 mRNA expression and downregulated CDK2 mRNA expression,leading to cell cycle arrest.Moreover,increased BAX/Bcl-2 ratio as well as caspase-9 and-3 were observed after treatment with Hydrangea serrata extract,indicating the induction of tumor cell apoptosis.Conclusions:Hydrangea serrata extract has the potential to alleviate tumors by effectively modulating cell-cycle-related gene expressions and inducing apoptosis,thereby inhibiting tumor growth.
文摘为深入研究绣球属植物花粉形态的分类学价值和系统学意义,厘清绣球属与近缘属之间的系统发育关系,该文利用扫描电子显微镜(SEM,scanning electron microscope)对国产绣球属及其近缘属41种绣球花科(Hydrangeaceae)植物的花粉形态以及表面纹饰进行了观察。结果表明:绣球属及其近缘属的花粉为三孔沟;形状多数为长圆体形或近球体形;赤道面观为椭圆形或圆形;极面观多为圆形,少数为三角形或圆三角形。花粉外壁纹饰可分为网状和孔穴状。网眼内的三级纹饰可分为光滑和具颗粒状突起。根据花粉形状和外壁纹饰类型将上述物种划分为4个组,即花粉的形状为长圆体形,表面纹饰为孔穴纹饰;花粉的形状为长圆体形,表面纹饰为网状纹饰;花粉的形状为近球体形,表面纹饰为孔穴纹饰;花粉的形状为近球体形,表面纹饰为网状纹饰。以上可进一步细分为8个类型。上述表明花粉形态证据可为绣球属及其近缘属的属下分类和种间界定提供重要佐证;但结合前人的系统发育重建分析该属植物花粉形态的系统学意义相对有限,如花粉形态证据对于该属及其近缘种属系统发育树上大支的界定难以提供有力的证据。
基金This research is supported by Science and Technology Research Project of Shanghai Greening and City Appearance Administration in 2019(G192402).
文摘Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions.In this study,nitrogen requirements for producing Hydrangea macrophylla‘Hanatemari’that robust seedlings,nutrient dynamics,biomass and growth,was examined utilizing exponential fertilization.The potted seedlings were fertilized with urea under exponential regime at rates of 0.5,1.5 and 2.0 g nitrogen/plant(EF1,EF2,and EF3),respectively.In addition,an unfertilized group treated with equal volume of deionized water was used as control.The results showed that seedlings under 1.5 g N/plant(EF2)had the highest plant growth index and total biomass.The nutrient concentrations of different organs varied in different fertilization treatments.Based on the results of current study,it is concluded that 1.5 g N/plant(EF2)is suitable exponential fertilization treatment for the culture of hydrangea seedlings.Our treatments results showed that 2.0 g N/plant is not suitable for seedling culturing,because of serious nutrient toxicity.These findings will help to improve seedling quality and strengthen the production of H.macrophylla for plantation.
基金This work is supported by the Grassland Talent Project:The Innovation Team of New Varieties Breeding at the Economic and Ecological Shrub,and the Evaluation of the Economic and Ecological Shrub Resources and New Variety Breeding in Inner Mongolia(No.201702077)。
文摘Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we established an efficient regeneration system by using stem segments as explants for the first time.In our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root induction.We found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(GA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)IBA.In summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.
文摘为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Komachi’)、‘佳澄’(‘Kasumi’)、‘无尽夏’(‘Endless Summer’)和不耐寒品种‘甜蜜幻想’(‘Sweet Fantasy’)、‘灵感’(‘Inspiration’)、‘你我的情感’(‘You and Me Feelings’)叶的抗氧化酶活性及丙二醛(MDA)和渗透调节物质含量进行比较,并对这些耐寒指标进行相关性分析和主成分分析。结果表明:供试绣球品种LT 50的波动较大,变化范围为-36.68℃~-1.87℃,其中,‘小町’的LT_(50)最低,‘佳澄’、‘平瓣’(‘Flat’)、‘蒂亚娜’(‘Tijana’)、‘塞尔玛’(‘Selma’)、‘含羞叶’(‘Elbtal’)、‘花手鞠’(‘Stockings’)、‘蓝色多瑙河’(‘Blue Danube’)和‘无尽夏’的LT_(50)均低于-20℃。40个绣球品种的耐寒性被分成不耐寒、较耐寒、耐寒和强耐寒4个等级,分别包含13、18、8和1个品种。6个代表品种的过氧化氢酶(CAT)、多酚氧化酶(PPO)和超氧化物歧化酶活性及MDA、脯氨酸(Pro)、可溶性蛋白质(SP)和可溶性糖含量随温度降低基本呈先升高后降低的变化趋势。相关性分析结果表明PPO活性和Pro含量与LT_(50)呈极显著(P<0.01)负相关。主成分分析结果表明:前4个主成分的累计贡献率为81.212%,说明这4个主成分基本能够反映绣球品种的耐寒性;PPO活性、CAT活性、Pro含量和SP含量的负荷量绝对值分别在第1、第2、第3和第4主成分中最高。研究结果显示:供试绣球品种间的耐寒性差异较大,‘小町’等9个品种的耐寒性较强,能够在冬季温度偏低地区露天栽培,并可作为绣球耐寒品种选育的亲本。PPO活性、CAT活性、Pro含量和SP含量为绣球耐寒性评价的主要指标,尤其是PPO活性和Pro含量。
