Human toxoplasmosis is caused by the intracellular protozoan parasite Toxoplasma gondii. Although T. gondii infection is generally asymptomatic for most of the immunocompetent adults, severe complications may occur pa...Human toxoplasmosis is caused by the intracellular protozoan parasite Toxoplasma gondii. Although T. gondii infection is generally asymptomatic for most of the immunocompetent adults, severe complications may occur particularly in pregnant women and immunocompromised individual. Host cell immunity plays a critical role in parasite differentiation and persistence in the host. Therefore, genetic polymorphism in the host immune genes, for instance interferon-γ gene could be linked with possibility of T. gondii infection. The objective of the study was to verify the link between the single nucleotide polymorphisms (SNPs) in the IFN-γ gene of pregnant women and T. gondii infection through correlating with anthropometric and sociodemographic parameters. In this study, ninety-two (N = 92) pregnant women (16 - 40 years) and healthy controls (N = 95) with similar age ranges were included. Among them, 25% (n = 23) pregnant women were seropositive for T. gondii IgG antibodies by Rapid Test Assay. Allelic and genotypic frequencies of IFN-γ +874T/A (rs2430561) SNPs were evaluated by using ARMS-PCR. The distribution of the A and T alleles in the specific position of the IFN-γ gene in the T. gondii-infected pregnant women and the control groups did not differ significantly, according to the data. However, we found a higher frequency (13.04%) of A/A genotype in T. gondii infected pregnant women as compared to non-infected individuals (8.70%), demonstrating that T. gondii infection susceptibility may be increased by homozygosity for the A allele. Further studies are to be needed to find out the link between host gene polymorphism and T. gondii infection in Bangladesh.展开更多
In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five pri...In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five principles of drug-like properties.With strong efficacy and pharmacological activity,HT has high drug-likeness degree.With good bioavailability,it can be easily absorbed by the gastrointestinal tract,though not absorbed by skin.Hydroxytyrosol has not only a strong potency and pharmacological activity,but also no liver toxicity and skin allergy.Tox data predicts that it has mutagenic potential,which may be the result of overreduction.展开更多
文摘Human toxoplasmosis is caused by the intracellular protozoan parasite Toxoplasma gondii. Although T. gondii infection is generally asymptomatic for most of the immunocompetent adults, severe complications may occur particularly in pregnant women and immunocompromised individual. Host cell immunity plays a critical role in parasite differentiation and persistence in the host. Therefore, genetic polymorphism in the host immune genes, for instance interferon-γ gene could be linked with possibility of T. gondii infection. The objective of the study was to verify the link between the single nucleotide polymorphisms (SNPs) in the IFN-γ gene of pregnant women and T. gondii infection through correlating with anthropometric and sociodemographic parameters. In this study, ninety-two (N = 92) pregnant women (16 - 40 years) and healthy controls (N = 95) with similar age ranges were included. Among them, 25% (n = 23) pregnant women were seropositive for T. gondii IgG antibodies by Rapid Test Assay. Allelic and genotypic frequencies of IFN-γ +874T/A (rs2430561) SNPs were evaluated by using ARMS-PCR. The distribution of the A and T alleles in the specific position of the IFN-γ gene in the T. gondii-infected pregnant women and the control groups did not differ significantly, according to the data. However, we found a higher frequency (13.04%) of A/A genotype in T. gondii infected pregnant women as compared to non-infected individuals (8.70%), demonstrating that T. gondii infection susceptibility may be increased by homozygosity for the A allele. Further studies are to be needed to find out the link between host gene polymorphism and T. gondii infection in Bangladesh.
基金This work was supported by China Postdoctoral Science Foundation(2020M670675).
文摘In order to study the possibility of hydroxytyrosol(HT)as a drug,we used SwissADME system to predict ADME of HT and pkCSM system to predict Tox of HT.The results show that hydroxytyrosol meets the Lipinski’s five principles of drug-like properties.With strong efficacy and pharmacological activity,HT has high drug-likeness degree.With good bioavailability,it can be easily absorbed by the gastrointestinal tract,though not absorbed by skin.Hydroxytyrosol has not only a strong potency and pharmacological activity,but also no liver toxicity and skin allergy.Tox data predicts that it has mutagenic potential,which may be the result of overreduction.
