Lung cancer has the highest mortality rate among all cancers,in part because it readily metastasizes.The tumor microenvironment,comprising blood vessels,fibroblasts,immune cells,and macrophages[including tumor-associa...Lung cancer has the highest mortality rate among all cancers,in part because it readily metastasizes.The tumor microenvironment,comprising blood vessels,fibroblasts,immune cells,and macrophages[including tumor-associated macrophages(TAMs)],is closely related to cancer cell growth,migration,and invasion.TAMs secrete several cytokines,including interleukin(IL)-1β,which participate in cancer migration and invasion.p21-activated kinase 1(PAK1),an important signaling molecule,induces cell migration and invasion in several carcinomas.Tonicityresponsive enhancer-binding protein(TonEBP)is also known to participate in cancer cell growth,migration,and invasion.However,the mechanisms by which it increases lung cancer migration remain unclear.Therefore,in this study,we aimed to elucidate the mechanisms by which IL-1βand TonEBP affect lung cancer cell migration and invasion.We found that A549 cocultured-MΦ-secreted IL-1βinduced A549 cell migration and invasion via the PAK1 pathway.TonEBP deficiency reduced A549 cell migration and invasion and increased responsiveness to IL-1β–induced migration and invasion.PAK1 phosphorylation,which was promoted by IL-1β,was reduced when TonEBP was depleted.These results suggest that TonEBP plays an important role in IL-1βinduction and invasiveness of A549 cells via the PAK1 pathway.These findings could be valuable in identifying potential targets for lung cancer treatment.展开更多
Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Generation 3 rat chondrocytes were randomized into Control...Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Generation 3 rat chondrocytes were randomized into Control,IL-1β,IL-1β+BSBT(L),IL-1β+BSBT(M),and IL-1β+BSBT(H)groups(5%,10%and 15%BSBT-containing serum),and then 24h after intervention respectively,the cell proliferation and Apoptosis rate;Western blot detected the expression levels of Bcl-2,BAX,Caspase-3,SOX9,NF-κB p65,MMP-13 proteins in chondrocytes.ELISA detected the levels of TNF-α,IL-6,and bFGF in the supernatants of chondrocyte culture.Results:Compared with Control group,cell proliferation activity decreased,apoptosis rate increased,NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level increased,and SOX9 protein level and bFGF level decreased in IL-1βgroup;compared with IL-1βgroup,different concentrations of BSBT-containing serum group,cell proliferation activity increased,and apoptosis rate decreased.NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level decreased,SOX9 protein level and bFGF level increased;compared with IL-1β+BSBT(L)group,cell proliferation activity increased,apoptosis rate decreased in IL-1β+BSBT(M)and IL-1β+BSBT(H)groups,and NF-κB p65,MMP-13 protein level and TNF-αlevel decreased.13 protein levels and TNF-αand IL-6 levels decreased,and SOX9 protein levels and bFGF levels increased.Conclusion:BSBT-containing serum may promote IL-1β-induced proliferation of chondrocytes,reduce apoptosis,improve the microenvironment of chondrocytes,and promote cartilage repair through the SOX9/NF-κB/MMP-13 signaling pathway.展开更多
目的:探讨低强度激光(LILT)对正畸牙齿移动的疗效及龈沟液白细胞介素-1β(IL-1β),核因子κB受体激活剂配体(RANKL)和骨保护素(OPG)水平的影响。方法:纳入正畸治疗患者60例,随机分为试验组和对照组各30例,对照组使用牙齿正畸治疗,实验...目的:探讨低强度激光(LILT)对正畸牙齿移动的疗效及龈沟液白细胞介素-1β(IL-1β),核因子κB受体激活剂配体(RANKL)和骨保护素(OPG)水平的影响。方法:纳入正畸治疗患者60例,随机分为试验组和对照组各30例,对照组使用牙齿正畸治疗,实验组加用LILT治疗。比较治疗后牙齿累积移动距离(CMD)和平均回缩速度(ARS),以及不同时间段疼痛VAS评分和龈沟液IL-1β,RANKL和OPG水平。结果:试验组7、14、21 d,28 d牙齿CMD均大于对照组(P<0.05),7、14和21 d ARS均高于对照组(P<0.05),治疗后4、24 h,7、14、21 d VAS评分均低于对照组(P<0.05),7 d OPG低于对照组,7、14和21 d RANKL高于对照组,21 d和28 d IL-1β高于对照组,7 d RANKL/OPG比率高于对照组(均P<0.05)。结论:LILT可通过影响骨代谢加速正畸牙齿的移动,并缓解治疗过程中的牙齿疼痛与牙周炎症。展开更多
目的:观察四妙丸对高尿酸性肾病患者血尿酸水平、肾功能及血清IL-1β、IL-18炎症因子分泌的影响。方法:选择90例高尿酸性肾病患者,随机分为非布司他组、四妙丸组和综合治疗组(非布司他+四妙丸),监测每组患者血尿酸、血肌酐水平,同时利用...目的:观察四妙丸对高尿酸性肾病患者血尿酸水平、肾功能及血清IL-1β、IL-18炎症因子分泌的影响。方法:选择90例高尿酸性肾病患者,随机分为非布司他组、四妙丸组和综合治疗组(非布司他+四妙丸),监测每组患者血尿酸、血肌酐水平,同时利用Elisa试剂盒测定血清IL-1β、IL-18炎症因子的分泌水平。结果:(1)血尿酸水平方面,非布司他组、综合治疗组2月时与0月相比即差异有统计学意义(414.03±41.21 vs 457.70±55.67,416.26±41.57 vs 486.56±63.32,P<0.05);四妙丸组3月时与0月比较差异有统计学意义(389.26±62.59 vs 456.23±66.77,P<0.05);3月时,综合治疗组与四妙丸组比较下降明显(353.0±70.12 vs 389.26.65±62.59,P<0.05)。(2)血肌酐水平方面,综合治疗组2月时与0月比较即明显降低(245.83±73.0 vs 311.76±88.48,P<0.05);四妙丸组3月时与0月比较显著下降(239.30±76.11 vs 291.50±94.72,P<0.05);3月时,与非布司他组相比,综合治疗组肌酐水平下降明显(209.9±56.62 vs 252.70±73.37,P<0.05);(3)血清炎症因子方面,非布司他组、四妙丸组、综合治疗组与0月时比较血清IL-1β水平(36.20±3.97 vs 40.0±5.89,29.66±7.62 vs 40.16±6.59,23.46±3.71 vs 41.63±4.88,P<0.05)、IL-18水平(67.13±3.27 vs 81.10±5.29,58.73±6.08 vs 78.76±4.90,49.93±5.13 vs 80.30±5.34,P<0.05)均显著下降;3月时,与非布司他组相比,四妙丸组、综合治疗组血清IL-1β水平(29.66±7.62 vs 36.20±3.97,23.46±3.71 vs 36.20±3.97,P<0.05)、IL-18水平(58.73±6.08 vs 67.13±3.27,49.93±5.13 vs 67.13±3.27,P<0.05)下降更为明显。结论:四妙丸能够通过降低血尿酸水平及抑制血清IL-1β、IL-18炎症因子的分泌对高尿酸性肾病患者产生肾脏保护作用。展开更多
基金the National Research Foundation of Korea(NRF)funded by the Ministry of Education(NRF-2014R1A6A1029617).
文摘Lung cancer has the highest mortality rate among all cancers,in part because it readily metastasizes.The tumor microenvironment,comprising blood vessels,fibroblasts,immune cells,and macrophages[including tumor-associated macrophages(TAMs)],is closely related to cancer cell growth,migration,and invasion.TAMs secrete several cytokines,including interleukin(IL)-1β,which participate in cancer migration and invasion.p21-activated kinase 1(PAK1),an important signaling molecule,induces cell migration and invasion in several carcinomas.Tonicityresponsive enhancer-binding protein(TonEBP)is also known to participate in cancer cell growth,migration,and invasion.However,the mechanisms by which it increases lung cancer migration remain unclear.Therefore,in this study,we aimed to elucidate the mechanisms by which IL-1βand TonEBP affect lung cancer cell migration and invasion.We found that A549 cocultured-MΦ-secreted IL-1βinduced A549 cell migration and invasion via the PAK1 pathway.TonEBP deficiency reduced A549 cell migration and invasion and increased responsiveness to IL-1β–induced migration and invasion.PAK1 phosphorylation,which was promoted by IL-1β,was reduced when TonEBP was depleted.These results suggest that TonEBP plays an important role in IL-1βinduction and invasiveness of A549 cells via the PAK1 pathway.These findings could be valuable in identifying potential targets for lung cancer treatment.
基金National Natural Science Foundation of China(No.82360934)Science and Technology Innovation Leading Talents Project of Xinjiang Uygur Autonomous Region(No.2022TSYCLJ0007)+1 种基金Xinjiang Uygur Autonomous Region Key Research and Development Task Special Project(No.2021B03006)Natural Science Foundat ion of Xinj iang Uygur Autonomous Region(No.2022D01C170,2022D01C171)。
文摘Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Generation 3 rat chondrocytes were randomized into Control,IL-1β,IL-1β+BSBT(L),IL-1β+BSBT(M),and IL-1β+BSBT(H)groups(5%,10%and 15%BSBT-containing serum),and then 24h after intervention respectively,the cell proliferation and Apoptosis rate;Western blot detected the expression levels of Bcl-2,BAX,Caspase-3,SOX9,NF-κB p65,MMP-13 proteins in chondrocytes.ELISA detected the levels of TNF-α,IL-6,and bFGF in the supernatants of chondrocyte culture.Results:Compared with Control group,cell proliferation activity decreased,apoptosis rate increased,NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level increased,and SOX9 protein level and bFGF level decreased in IL-1βgroup;compared with IL-1βgroup,different concentrations of BSBT-containing serum group,cell proliferation activity increased,and apoptosis rate decreased.NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level decreased,SOX9 protein level and bFGF level increased;compared with IL-1β+BSBT(L)group,cell proliferation activity increased,apoptosis rate decreased in IL-1β+BSBT(M)and IL-1β+BSBT(H)groups,and NF-κB p65,MMP-13 protein level and TNF-αlevel decreased.13 protein levels and TNF-αand IL-6 levels decreased,and SOX9 protein levels and bFGF levels increased.Conclusion:BSBT-containing serum may promote IL-1β-induced proliferation of chondrocytes,reduce apoptosis,improve the microenvironment of chondrocytes,and promote cartilage repair through the SOX9/NF-κB/MMP-13 signaling pathway.
文摘目的:探讨低强度激光(LILT)对正畸牙齿移动的疗效及龈沟液白细胞介素-1β(IL-1β),核因子κB受体激活剂配体(RANKL)和骨保护素(OPG)水平的影响。方法:纳入正畸治疗患者60例,随机分为试验组和对照组各30例,对照组使用牙齿正畸治疗,实验组加用LILT治疗。比较治疗后牙齿累积移动距离(CMD)和平均回缩速度(ARS),以及不同时间段疼痛VAS评分和龈沟液IL-1β,RANKL和OPG水平。结果:试验组7、14、21 d,28 d牙齿CMD均大于对照组(P<0.05),7、14和21 d ARS均高于对照组(P<0.05),治疗后4、24 h,7、14、21 d VAS评分均低于对照组(P<0.05),7 d OPG低于对照组,7、14和21 d RANKL高于对照组,21 d和28 d IL-1β高于对照组,7 d RANKL/OPG比率高于对照组(均P<0.05)。结论:LILT可通过影响骨代谢加速正畸牙齿的移动,并缓解治疗过程中的牙齿疼痛与牙周炎症。
文摘目的:观察四妙丸对高尿酸性肾病患者血尿酸水平、肾功能及血清IL-1β、IL-18炎症因子分泌的影响。方法:选择90例高尿酸性肾病患者,随机分为非布司他组、四妙丸组和综合治疗组(非布司他+四妙丸),监测每组患者血尿酸、血肌酐水平,同时利用Elisa试剂盒测定血清IL-1β、IL-18炎症因子的分泌水平。结果:(1)血尿酸水平方面,非布司他组、综合治疗组2月时与0月相比即差异有统计学意义(414.03±41.21 vs 457.70±55.67,416.26±41.57 vs 486.56±63.32,P<0.05);四妙丸组3月时与0月比较差异有统计学意义(389.26±62.59 vs 456.23±66.77,P<0.05);3月时,综合治疗组与四妙丸组比较下降明显(353.0±70.12 vs 389.26.65±62.59,P<0.05)。(2)血肌酐水平方面,综合治疗组2月时与0月比较即明显降低(245.83±73.0 vs 311.76±88.48,P<0.05);四妙丸组3月时与0月比较显著下降(239.30±76.11 vs 291.50±94.72,P<0.05);3月时,与非布司他组相比,综合治疗组肌酐水平下降明显(209.9±56.62 vs 252.70±73.37,P<0.05);(3)血清炎症因子方面,非布司他组、四妙丸组、综合治疗组与0月时比较血清IL-1β水平(36.20±3.97 vs 40.0±5.89,29.66±7.62 vs 40.16±6.59,23.46±3.71 vs 41.63±4.88,P<0.05)、IL-18水平(67.13±3.27 vs 81.10±5.29,58.73±6.08 vs 78.76±4.90,49.93±5.13 vs 80.30±5.34,P<0.05)均显著下降;3月时,与非布司他组相比,四妙丸组、综合治疗组血清IL-1β水平(29.66±7.62 vs 36.20±3.97,23.46±3.71 vs 36.20±3.97,P<0.05)、IL-18水平(58.73±6.08 vs 67.13±3.27,49.93±5.13 vs 67.13±3.27,P<0.05)下降更为明显。结论:四妙丸能够通过降低血尿酸水平及抑制血清IL-1β、IL-18炎症因子的分泌对高尿酸性肾病患者产生肾脏保护作用。
