[Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8m RNA by LPS-injured IEC-6 cells,in order to figure out the mechanism of EPS on injured cells. [Method]Total RNA was ext...[Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8m RNA by LPS-injured IEC-6 cells,in order to figure out the mechanism of EPS on injured cells. [Method]Total RNA was extracted with TRIzon reagent. IL-8 m RNA was amplified by RT-PCR and detected by agarose gel electrophoresis. Furthermore,electrophoresis and image was analyzed. [Result] The 50 μg/m L EPS could partially inhibit IL-8 m RNA level produced by LPS-stimulated IEC-6; with the increasing concentration of EPS(200 and 500 μg/m L),the inhibitory effect against IL-8 m RNA gradually enhanced. IEC-6 was pretreated by 50,100,200 and 500 μg/m L EPS for 24 h,then stimulated by 10 μg/m L LPS for 1 and 4 h,respectively. RT-PCR analysis showed that the expression of IL-8 m RNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8m RNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at 1 h. [Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 m RNA by LPS-stimulated cells,and the inhibition of EPS was dependent to concentration and time.展开更多
基金Supported by National Natural Science Foundation of China(31472230)Natural Science Foundation of Hebei Province(C2014407068)Project of Hebei Department of Science and Technology(14966610D)
文摘[Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8m RNA by LPS-injured IEC-6 cells,in order to figure out the mechanism of EPS on injured cells. [Method]Total RNA was extracted with TRIzon reagent. IL-8 m RNA was amplified by RT-PCR and detected by agarose gel electrophoresis. Furthermore,electrophoresis and image was analyzed. [Result] The 50 μg/m L EPS could partially inhibit IL-8 m RNA level produced by LPS-stimulated IEC-6; with the increasing concentration of EPS(200 and 500 μg/m L),the inhibitory effect against IL-8 m RNA gradually enhanced. IEC-6 was pretreated by 50,100,200 and 500 μg/m L EPS for 24 h,then stimulated by 10 μg/m L LPS for 1 and 4 h,respectively. RT-PCR analysis showed that the expression of IL-8 m RNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8m RNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at 1 h. [Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 m RNA by LPS-stimulated cells,and the inhibition of EPS was dependent to concentration and time.
