Osteoarthritis(OA)is a debilitating degenerative disease affecting multiple joint tissues,including cartilage,bone,synovium,and adipose tissues.OA presents diverse clinical phenotypes and distinct molecular endotypes,...Osteoarthritis(OA)is a debilitating degenerative disease affecting multiple joint tissues,including cartilage,bone,synovium,and adipose tissues.OA presents diverse clinical phenotypes and distinct molecular endotypes,including inflammatory,metabolic,mechanical,genetic,and synovial variants.Consequently,innovative technologies are needed to support the development of effective diagnostic and precision therapeutic approaches.Traditional analysis of bulk OA tissue extracts has limitations due to technical constraints,causing challenges in the differentiation between various physiological and pathological phenotypes in joint tissues.This issue has led to standardization difficulties and hindered the success of clinical trials.Gaining insights into the spatial variations of the cellular and molecular structures in OA tissues,encompassing DNA,RNA,metabolites,and proteins,as well as their chemical properties,elemental composition,and mechanical attributes,can contribute to a more comprehensive understanding of the disease subtypes.Spatially resolved biology enables biologists to investigate cells within the context of their tissue microenvironment,providing a more holistic view of cellular function.Recent advances in innovative spatial biology techniques now allow intact tissue sections to be examined using various-omics lenses,such as genomics,transcriptomics,proteomics,and metabolomics,with spatial data.This fusion of approaches provides researchers with critical insights into the molecular composition and functions of the cells and tissues at precise spatial coordinates.Furthermore,advanced imaging techniques,including high-resolution microscopy,hyperspectral imaging,and mass spectrometry imaging,enable the visualization and analysis of the spatial distribution of biomolecules,cells,and tissues.Linking these molecular imaging outputs to conventional tissue histology can facilitate a more comprehensive characterization of disease phenotypes.This review summarizes the recent advancements in the molecular imaging modalities and methodologies for in-depth spatial analysis.It explores their applications,challenges,and potential opportunities in the field of OA.Additionally,this review provides a perspective on the potential research directions for these contemporary approaches that can meet the requirements of clinical diagnoses and the establishment of therapeutic targets for OA.展开更多
With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether th...With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether they are physiological or pathological.展开更多
Very long chain-saturated and-polyunsaturated fatty acids(VLC-SFA and VLC-PUFA, respectively) are a functionally important class of fatty acids containing 28 carbons or more in their acyl chain. They are synthesized b...Very long chain-saturated and-polyunsaturated fatty acids(VLC-SFA and VLC-PUFA, respectively) are a functionally important class of fatty acids containing 28 carbons or more in their acyl chain. They are synthesized by the elongation of very long fatty acids-4(ELOVL4) enzyme, expressed mainly in the brain, retina, skin, testes, and meibomian gland, where these fatty acids are found(Agbaga et al., 2008). Further, these organs exhibit tissuespecific VLC-PUFA and VLC-SFA biosynthesis and incorporation into complex lipids for specific functions. In the brain, skin, and Meibomian glands, the ELOVL4 mainly makes VLC-SFA, which are incorporated into complex sphingolipids. In the retina, the ELOVL4 makes VLC-PUFA that are incorporated into phosphatidylcholine, that are critical for visual function, while in testes and sperm, the VLC-PUFA are incorporated into sphingolipids that are critical for fertility(Yeboah et al., 2021).展开更多
Ageing as a natural irreversible process inherently results in the functional deterioration of numerous organ systems and tissues,including the skeletal and immune systems.Recent studies have elucidated the intricate ...Ageing as a natural irreversible process inherently results in the functional deterioration of numerous organ systems and tissues,including the skeletal and immune systems.Recent studies have elucidated the intricate bidirectional interactions between these two systems.展开更多
DEAR EDITOR,The Laosicheng site in Yongshun County,Hunan Province,China,plays an important role in elucidating the animal population composition of the region prior to the middle Qing Dynasty(approximately 300 years a...DEAR EDITOR,The Laosicheng site in Yongshun County,Hunan Province,China,plays an important role in elucidating the animal population composition of the region prior to the middle Qing Dynasty(approximately 300 years ago).This site has yielded a substantial number of animal bones,11960 of which have been identified to the species level,serving as direct evidence of ancient animal populations(Hunan Provincial Institute of Cultural Relics and Archaeology,2018).展开更多
The InSight(Interior Exploration using Seismic Investigations,Geodesy,and Heat Transport)mission has recorded continuous ambient noise signals with many spectral peaks since its landing in 2018.The majority of these p...The InSight(Interior Exploration using Seismic Investigations,Geodesy,and Heat Transport)mission has recorded continuous ambient noise signals with many spectral peaks since its landing in 2018.The majority of these peaks are modes produced by instrumental vibrations and are associated with environmental factors,such as temperature and wind energy fluctuations.Understanding how these modes react under various conditions is crucial because it aids in identifying their origins.In this study,we analyzed the three-component spectra of InSight recordings from sols 184–738 and obtained the horizontal-to-vertical spectral ratio(HVSR,also known as H/V)curves for different time intervals.The primary modes,such as those at 3.3 and 4.1 Hz,exhibited different behaviors,suggesting diverse origins.Some modes were sensitive to low temperature and some were sensitive to high temperature.Additionally,we investigated the influence of wind and temperature on the H/V curve.The peak frequency was mainly affected by temperature,whereas the H/V value was not associated with the temperature or wind only.Characterizing these modes and elucidating their origins are significant for processing signals from InSight and can provide valuable guidance for designing future planetary seismometers.展开更多
In the context of the cultural development of the new era and the burgeoning spiritual demands of the people,there has been a remarkable surge in the proliferation of traditional Chinese cultural programs.The“Chinese...In the context of the cultural development of the new era and the burgeoning spiritual demands of the people,there has been a remarkable surge in the proliferation of traditional Chinese cultural programs.The“Chinese Festivals”series,produced by Henan TV,standing out as an engaging TV program that showcases traditional Chinese culture,has rapidly captivated a diverse audience across different age groups,creating a rich,multi-dimensional,and cross-media cultural communication ecosystem.The program strategically selects symbolic elements by systematically categorizing and organizing traditional Chinese cultural symbols to create a rich cultural space.It employs cutting-edge scientific and technological methods to enrich viewer immersion,crafting media rituals with both temporal significance and a profound sense of media ritual.This approach boosts the program’s popularity and sets a benchmark for industry innovation.By fostering an interactive narrative between mainstream media and the public,the program continuously broadens the scope of the“story world,”fostering a harmonious and structured national cultural community through the creative interpretation of traditional Chinese culture and the evocative crafting of the aesthetic sensibilities inherent in Chinese art.It is worth noting that the phenomenon of aesthetic fatigue besets the development of this serial program.To address this issue and further improve the appeal of the program,a comprehensive approach is required to develop a cohesive narrative mode for the“story world.”This involves harmonizing the interplay between artistic endeavors and technological advancements,synchronizing sensory experiences with the foundational ritualistic elements,and enhancing the effectiveness of the media communication matrix to construct a brand of media culture that has social significance,aesthetic value,and economic profits in the new era.展开更多
In the era of stock development following the acceleration of urbanization,the revitalization of urban green space has assumed an increasingly significant role.Consequently,the management of urban trees has emerged as...In the era of stock development following the acceleration of urbanization,the revitalization of urban green space has assumed an increasingly significant role.Consequently,the management of urban trees has emerged as a critical focus of urban governance,contributing to the enhancement of livability in human settlements.This study offers a comprehensive analysis of the urban tree management system in Oxford,UK,identifying that its primary objective is to optimize and maintain a harmonious balance between human activities and the natural environment through the implementation of high-quality planting practices.The system emphasizes enhanced management practices and establishes a robust framework for the development of targeted policies and management regulations,utilizing i-Tree eco-efficiency assessment and real-time feedback mechanisms.China’s urban tree management is in its nascent stages,and there is an urgent need for the development of urban green space.By adopting the refined management assessment methodologies employed for urban trees in Oxford,UK,it is possible to enhance the ecological value of urban trees,which represent a significant green resource within cities,and contribute to the creation of more livable urban spaces.展开更多
China’s annual Two Sessions have again attracted worldwide attention.A close look at the Two Sessions always provides key insights on China.China is often accused of being undemocratic.In fact,however,China is democr...China’s annual Two Sessions have again attracted worldwide attention.A close look at the Two Sessions always provides key insights on China.China is often accused of being undemocratic.In fact,however,China is democratic in practice except that its democracy differs from systems studied in the West or in general politics.China’s democratic process is worth studying.展开更多
Customer churn poses a significant challenge for the banking and finance industry in the United States, directly affecting profitability and market share. This study conducts a comprehensive comparative analysis of ma...Customer churn poses a significant challenge for the banking and finance industry in the United States, directly affecting profitability and market share. This study conducts a comprehensive comparative analysis of machine learning models for customer churn prediction, focusing on the U.S. context. The research evaluates the performance of logistic regression, random forest, and neural networks using industry-specific datasets, considering the economic impact and practical implications of the findings. The exploratory data analysis reveals unique patterns and trends in the U.S. banking and finance industry, such as the age distribution of customers and the prevalence of dormant accounts. The study incorporates macroeconomic factors to capture the potential influence of external conditions on customer churn behavior. The findings highlight the importance of leveraging advanced machine learning techniques and comprehensive customer data to develop effective churn prevention strategies in the U.S. context. By accurately predicting customer churn, financial institutions can proactively identify at-risk customers, implement targeted retention strategies, and optimize resource allocation. The study discusses the limitations and potential future improvements, serving as a roadmap for researchers and practitioners to further advance the field of customer churn prediction in the evolving landscape of the U.S. banking and finance industry.展开更多
The lemon(Citrus limon;family Rutaceae)is one of the most important and popular fruits worldwide.Lemon also tolerates huan-glongbing(HLB)disease,which is a devastating citrus disease.Here we produced a gap-free and ha...The lemon(Citrus limon;family Rutaceae)is one of the most important and popular fruits worldwide.Lemon also tolerates huan-glongbing(HLB)disease,which is a devastating citrus disease.Here we produced a gap-free and haplotype-resolved chromosome-scale genome assembly of the lemon by combining Pacific Biosciences circular consensus sequencing,Oxford Nanopore 50-kb ultra-long,and high-throughput chromatin conformation capture technologies.The assembly contained nine-pair chromosomes with a contig N50 of 35.6 Mb and zero gaps,while a total of 633.0 Mb genomic sequences were generated.The origination analysis identified 338.5Mb genomic sequences originating from citron(53.5%),147.4Mb frommandarin(23.3%),and 147.1Mb frompummelo(23.2%).The genome included 30528 protein-coding genes,and most of the assembled sequences were found to be repetitive sequences.Several significantly expanded gene families were associated with plant-pathogen interactions,plant hormone signal transduction,and the biosynthesis of major active components,such as terpenoids and f lavor compounds.Most HLB-tolerant genes were expanded in the lemon genome,such as 2-oxoglutarate(2OG)/Fe(II)-dependent oxygenase and constitutive disease resistance 1,cell wall-related genes,and lignin synthesis genes.Comparative transcriptomic analysis showed that phloem regeneration and lower levels of phloem plugging are the elements that contribute to HLB tolerance in lemon.Our results provide insight into lemon genome evolution,active component biosynthesis,and genes associated with HLB tolerance.展开更多
See related article,pp 357-363The first study describing the cell death and destruction of tissues,organs,and organ systems as programmed events during the development of multicellular organisms was performed by JW Sa...See related article,pp 357-363The first study describing the cell death and destruction of tissues,organs,and organ systems as programmed events during the development of multicellular organisms was performed by JW Saunders Jr in 1966.The term apoptosis was introduced by Kerr,Wyllie,and Currie in 1972 to describe a programmed phenomenon opposite to mitosis in the regulation of animal cell populations.Later,a description of ectodermal cell lineages as programmed cell death in the worm Caenorhabditis elegans was publishedby JE Sulston and HR Horvitz in 1976.展开更多
Okra(Abelmoschus esculentus)is an important vegetable crop with high nutritional value.However,the mechanism underlying its high nutrient content remains poorly understood.Here,we present a chromosome-scale genome of ...Okra(Abelmoschus esculentus)is an important vegetable crop with high nutritional value.However,the mechanism underlying its high nutrient content remains poorly understood.Here,we present a chromosome-scale genome of okra with a size of 1.19 Gb.Comparative genomics analysis revealed the phylogenetic status of A.esculentus,as well as whole-genome duplication(WGD)events that have occurred widely across the Malvaceae species.We found that okra has experienced three additional WGDs compared with the diploid cotton Gossypium raimondii,resulting in a large chromosome number(2n=130).After three WGDs,okra has undergone extensive genomic deletions and retained substantial numbers of genes related to secondary metabolite biosynthesis and environmental adaptation,resulting in significant differences between okra and G.raimondii in the gene families related to cellulose synthesis.Combining transcriptomic and metabolomic analysis,we revealed the relationship between gene expression and metabolite content change across different okra developmental stages.Furthermore,the sinapic acid/S-lignin biosynthesis-related gene families have experienced remarkable expansion in okra,and the expression of key enzymes involved in the sinapic acid/S-lignin biosynthesis pathway vary greatly across developmental periods,which partially explains the differences in metabolite content across the different stages.Our study gains insights into the comprehensive evolutionary history of Malvaceae species and the genetic basis that underlies the nutrient content changes in okra,which will facilitate the functional study and genetic improvement of okra varieties.展开更多
Polygala tenuifolia is a perennial medicinal plant that has been widely used in traditional Chinese medicine for treating mental diseases.However,the lack of genomic resources limits the insight into its evolutionary ...Polygala tenuifolia is a perennial medicinal plant that has been widely used in traditional Chinese medicine for treating mental diseases.However,the lack of genomic resources limits the insight into its evolutionary and biological characterization.In the present work,we reported the P.tenuifolia genome,the first genome assembly of the Polygalaceae family.We sequenced and assembled this genome by a combination of Illumnina,PacBio HiFi,and Hi-C mapping.The assembly includes 19 pseudochromosomes covering∼92.68%of the assembled genome(∼769.62 Mb).There are 36463 protein-coding genes annotated in this genome.Detailed comparative genome anal-ysis revealed that P.tenuifolia experienced two rounds of whole genome duplication that occurred∼39–44 and∼18–20 million years ago,respectively.Accordingly,we systematically reconstructed ancestral chromosomes of P.tenuifolia and inferred its chromosome evolution trajectories from the common ancestor of core eudicots to the present species.Based on the transcriptomics data,enzyme genes and transcription factors involved in the synthesis of triterpenoid saponin in P.tenuifolia were identified.Further analysis demonstrated that whole-genome duplications and tandem duplications play critical roles in the expansion of P450 and UGT gene families,which contributed to the synthesis of triterpenoid saponins.The genome and transcriptome data will not only provide valuable resources for comparative and functional genomic researches on Polygalaceae,but also shed light on the synthesis of triterpenoid saponin.展开更多
Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wil...Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.展开更多
How organizations analyze and use data for decision-making has been changed by cognitive computing and artificial intelligence (AI). Cognitive computing solutions can translate enormous amounts of data into valuable i...How organizations analyze and use data for decision-making has been changed by cognitive computing and artificial intelligence (AI). Cognitive computing solutions can translate enormous amounts of data into valuable insights by utilizing the power of cutting-edge algorithms and machine learning, empowering enterprises to make deft decisions quickly and efficiently. This article explores the idea of cognitive computing and AI in decision-making, emphasizing its function in converting unvalued data into valuable knowledge. It details the advantages of utilizing these technologies, such as greater productivity, accuracy, and efficiency. Businesses may use cognitive computing and AI to their advantage to obtain a competitive edge in today’s data-driven world by knowing their capabilities and possibilities [1].展开更多
Lipid droplets (LDs) are ubiquitous cellular orga nelles that perform functions mostly dedicated to energy homeostasis and lipid metabolism.As neutral lipid depots (triacylglycerol,sterol esters),they can be rapidly m...Lipid droplets (LDs) are ubiquitous cellular orga nelles that perform functions mostly dedicated to energy homeostasis and lipid metabolism.As neutral lipid depots (triacylglycerol,sterol esters),they can be rapidly mobilized through lipase-mediated hydrolysis (lipolysis) or via lipophagy,a specific form of autophagy devoted to consumption of LDs inside the lysosome.展开更多
基金the NHMRC Investigator grant fellowship (APP1176298)the EMCR grant from the Centre for Biomedical Technologies (QUT)+4 种基金the QUT Postgraduate Research Award (QUTPRA)QUT HDR TOP-UP scholarshipQUT HDR Tuition Fee Sponsorshipfunding support from the Academy of Finland (315820)the Jane and Aatos Erkko Foundation (190001).
文摘Osteoarthritis(OA)is a debilitating degenerative disease affecting multiple joint tissues,including cartilage,bone,synovium,and adipose tissues.OA presents diverse clinical phenotypes and distinct molecular endotypes,including inflammatory,metabolic,mechanical,genetic,and synovial variants.Consequently,innovative technologies are needed to support the development of effective diagnostic and precision therapeutic approaches.Traditional analysis of bulk OA tissue extracts has limitations due to technical constraints,causing challenges in the differentiation between various physiological and pathological phenotypes in joint tissues.This issue has led to standardization difficulties and hindered the success of clinical trials.Gaining insights into the spatial variations of the cellular and molecular structures in OA tissues,encompassing DNA,RNA,metabolites,and proteins,as well as their chemical properties,elemental composition,and mechanical attributes,can contribute to a more comprehensive understanding of the disease subtypes.Spatially resolved biology enables biologists to investigate cells within the context of their tissue microenvironment,providing a more holistic view of cellular function.Recent advances in innovative spatial biology techniques now allow intact tissue sections to be examined using various-omics lenses,such as genomics,transcriptomics,proteomics,and metabolomics,with spatial data.This fusion of approaches provides researchers with critical insights into the molecular composition and functions of the cells and tissues at precise spatial coordinates.Furthermore,advanced imaging techniques,including high-resolution microscopy,hyperspectral imaging,and mass spectrometry imaging,enable the visualization and analysis of the spatial distribution of biomolecules,cells,and tissues.Linking these molecular imaging outputs to conventional tissue histology can facilitate a more comprehensive characterization of disease phenotypes.This review summarizes the recent advancements in the molecular imaging modalities and methodologies for in-depth spatial analysis.It explores their applications,challenges,and potential opportunities in the field of OA.Additionally,this review provides a perspective on the potential research directions for these contemporary approaches that can meet the requirements of clinical diagnoses and the establishment of therapeutic targets for OA.
基金Clévio Nóbrega’s laboratory is funded by the Cure CSB projectthe Viljem Julijan Association for Children with Rare Diseases(Slovenia)+1 种基金the Algarve Biomedical Center Research Institute(ABC-Ri)funded by CRESC Algarve 2020(Operation Code:ALG-01-0145-FEDER-072586)(to CN)。
文摘With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether they are physiological or pathological.
基金supported by NEI/NIH R01 EY030513NIAMS/NIH R21-AR076035Multi-PI Team Science grant from Presbyterian Health Foundation。
文摘Very long chain-saturated and-polyunsaturated fatty acids(VLC-SFA and VLC-PUFA, respectively) are a functionally important class of fatty acids containing 28 carbons or more in their acyl chain. They are synthesized by the elongation of very long fatty acids-4(ELOVL4) enzyme, expressed mainly in the brain, retina, skin, testes, and meibomian gland, where these fatty acids are found(Agbaga et al., 2008). Further, these organs exhibit tissuespecific VLC-PUFA and VLC-SFA biosynthesis and incorporation into complex lipids for specific functions. In the brain, skin, and Meibomian glands, the ELOVL4 mainly makes VLC-SFA, which are incorporated into complex sphingolipids. In the retina, the ELOVL4 makes VLC-PUFA that are incorporated into phosphatidylcholine, that are critical for visual function, while in testes and sperm, the VLC-PUFA are incorporated into sphingolipids that are critical for fertility(Yeboah et al., 2021).
基金supported by the National Key R&D Program of China (2021YFA1101500)Wuhan Science and Technology Bureau (2022020801020464)+1 种基金partially supported by University Grants Committee,Research Grants Council of the Hong Kong Special Administrative Region,China (14113723,N_CUHK472/22,T13-402/17-N and AoE/M-402/20)Direct Grant of CUHK (2022.042)。
文摘Ageing as a natural irreversible process inherently results in the functional deterioration of numerous organ systems and tissues,including the skeletal and immune systems.Recent studies have elucidated the intricate bidirectional interactions between these two systems.
基金supported by the National Natural Science Foundation of China(41925009,32100488)National Key R&D Program of China(2022YFE0203800)+1 种基金Chinese Academy of Sciences(YSBR-019)New Cornerstone Science Foundation through the XPLORER PRIZE。
文摘DEAR EDITOR,The Laosicheng site in Yongshun County,Hunan Province,China,plays an important role in elucidating the animal population composition of the region prior to the middle Qing Dynasty(approximately 300 years ago).This site has yielded a substantial number of animal bones,11960 of which have been identified to the species level,serving as direct evidence of ancient animal populations(Hunan Provincial Institute of Cultural Relics and Archaeology,2018).
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB 41000000).
文摘The InSight(Interior Exploration using Seismic Investigations,Geodesy,and Heat Transport)mission has recorded continuous ambient noise signals with many spectral peaks since its landing in 2018.The majority of these peaks are modes produced by instrumental vibrations and are associated with environmental factors,such as temperature and wind energy fluctuations.Understanding how these modes react under various conditions is crucial because it aids in identifying their origins.In this study,we analyzed the three-component spectra of InSight recordings from sols 184–738 and obtained the horizontal-to-vertical spectral ratio(HVSR,also known as H/V)curves for different time intervals.The primary modes,such as those at 3.3 and 4.1 Hz,exhibited different behaviors,suggesting diverse origins.Some modes were sensitive to low temperature and some were sensitive to high temperature.Additionally,we investigated the influence of wind and temperature on the H/V curve.The peak frequency was mainly affected by temperature,whereas the H/V value was not associated with the temperature or wind only.Characterizing these modes and elucidating their origins are significant for processing signals from InSight and can provide valuable guidance for designing future planetary seismometers.
基金the project“Research on the Digitalized Living Inheritance and Development of the Yellow River Cultural Gene,”a project of Humanities and Social Sciences Research of Higher Education Institutes in Henan Province(Project No.2024-ZDJH-770).
文摘In the context of the cultural development of the new era and the burgeoning spiritual demands of the people,there has been a remarkable surge in the proliferation of traditional Chinese cultural programs.The“Chinese Festivals”series,produced by Henan TV,standing out as an engaging TV program that showcases traditional Chinese culture,has rapidly captivated a diverse audience across different age groups,creating a rich,multi-dimensional,and cross-media cultural communication ecosystem.The program strategically selects symbolic elements by systematically categorizing and organizing traditional Chinese cultural symbols to create a rich cultural space.It employs cutting-edge scientific and technological methods to enrich viewer immersion,crafting media rituals with both temporal significance and a profound sense of media ritual.This approach boosts the program’s popularity and sets a benchmark for industry innovation.By fostering an interactive narrative between mainstream media and the public,the program continuously broadens the scope of the“story world,”fostering a harmonious and structured national cultural community through the creative interpretation of traditional Chinese culture and the evocative crafting of the aesthetic sensibilities inherent in Chinese art.It is worth noting that the phenomenon of aesthetic fatigue besets the development of this serial program.To address this issue and further improve the appeal of the program,a comprehensive approach is required to develop a cohesive narrative mode for the“story world.”This involves harmonizing the interplay between artistic endeavors and technological advancements,synchronizing sensory experiences with the foundational ritualistic elements,and enhancing the effectiveness of the media communication matrix to construct a brand of media culture that has social significance,aesthetic value,and economic profits in the new era.
基金Beijing Urban Governance Research Base of North China University of Technology(2024CSZL07).
文摘In the era of stock development following the acceleration of urbanization,the revitalization of urban green space has assumed an increasingly significant role.Consequently,the management of urban trees has emerged as a critical focus of urban governance,contributing to the enhancement of livability in human settlements.This study offers a comprehensive analysis of the urban tree management system in Oxford,UK,identifying that its primary objective is to optimize and maintain a harmonious balance between human activities and the natural environment through the implementation of high-quality planting practices.The system emphasizes enhanced management practices and establishes a robust framework for the development of targeted policies and management regulations,utilizing i-Tree eco-efficiency assessment and real-time feedback mechanisms.China’s urban tree management is in its nascent stages,and there is an urgent need for the development of urban green space.By adopting the refined management assessment methodologies employed for urban trees in Oxford,UK,it is possible to enhance the ecological value of urban trees,which represent a significant green resource within cities,and contribute to the creation of more livable urban spaces.
文摘China’s annual Two Sessions have again attracted worldwide attention.A close look at the Two Sessions always provides key insights on China.China is often accused of being undemocratic.In fact,however,China is democratic in practice except that its democracy differs from systems studied in the West or in general politics.China’s democratic process is worth studying.
文摘Customer churn poses a significant challenge for the banking and finance industry in the United States, directly affecting profitability and market share. This study conducts a comprehensive comparative analysis of machine learning models for customer churn prediction, focusing on the U.S. context. The research evaluates the performance of logistic regression, random forest, and neural networks using industry-specific datasets, considering the economic impact and practical implications of the findings. The exploratory data analysis reveals unique patterns and trends in the U.S. banking and finance industry, such as the age distribution of customers and the prevalence of dormant accounts. The study incorporates macroeconomic factors to capture the potential influence of external conditions on customer churn behavior. The findings highlight the importance of leveraging advanced machine learning techniques and comprehensive customer data to develop effective churn prevention strategies in the U.S. context. By accurately predicting customer churn, financial institutions can proactively identify at-risk customers, implement targeted retention strategies, and optimize resource allocation. The study discusses the limitations and potential future improvements, serving as a roadmap for researchers and practitioners to further advance the field of customer churn prediction in the evolving landscape of the U.S. banking and finance industry.
基金supported by the Guangxi Major Project of Science and Technology(Guike AA18118027)the Postdoctoral Project of Hainan Yazhou Bay Seed Laboratory Program(B21Y10203)the Scientific Research and Development Fund of the College of Agriculture,Guangxi University(EE101731).
文摘The lemon(Citrus limon;family Rutaceae)is one of the most important and popular fruits worldwide.Lemon also tolerates huan-glongbing(HLB)disease,which is a devastating citrus disease.Here we produced a gap-free and haplotype-resolved chromosome-scale genome assembly of the lemon by combining Pacific Biosciences circular consensus sequencing,Oxford Nanopore 50-kb ultra-long,and high-throughput chromatin conformation capture technologies.The assembly contained nine-pair chromosomes with a contig N50 of 35.6 Mb and zero gaps,while a total of 633.0 Mb genomic sequences were generated.The origination analysis identified 338.5Mb genomic sequences originating from citron(53.5%),147.4Mb frommandarin(23.3%),and 147.1Mb frompummelo(23.2%).The genome included 30528 protein-coding genes,and most of the assembled sequences were found to be repetitive sequences.Several significantly expanded gene families were associated with plant-pathogen interactions,plant hormone signal transduction,and the biosynthesis of major active components,such as terpenoids and f lavor compounds.Most HLB-tolerant genes were expanded in the lemon genome,such as 2-oxoglutarate(2OG)/Fe(II)-dependent oxygenase and constitutive disease resistance 1,cell wall-related genes,and lignin synthesis genes.Comparative transcriptomic analysis showed that phloem regeneration and lower levels of phloem plugging are the elements that contribute to HLB tolerance in lemon.Our results provide insight into lemon genome evolution,active component biosynthesis,and genes associated with HLB tolerance.
基金supported by Neural Therapies SL(NT-Dev-01)University of León。
文摘See related article,pp 357-363The first study describing the cell death and destruction of tissues,organs,and organ systems as programmed events during the development of multicellular organisms was performed by JW Saunders Jr in 1966.The term apoptosis was introduced by Kerr,Wyllie,and Currie in 1972 to describe a programmed phenomenon opposite to mitosis in the regulation of animal cell populations.Later,a description of ectodermal cell lineages as programmed cell death in the worm Caenorhabditis elegans was publishedby JE Sulston and HR Horvitz in 1976.
基金This work was supported by the Natural Science Foundation of Hebei Province(Grant No.C2021201048)the Interdisciplinary Research Program of Natural Science of Hebei University(Grant No.513201422004)。
文摘Okra(Abelmoschus esculentus)is an important vegetable crop with high nutritional value.However,the mechanism underlying its high nutrient content remains poorly understood.Here,we present a chromosome-scale genome of okra with a size of 1.19 Gb.Comparative genomics analysis revealed the phylogenetic status of A.esculentus,as well as whole-genome duplication(WGD)events that have occurred widely across the Malvaceae species.We found that okra has experienced three additional WGDs compared with the diploid cotton Gossypium raimondii,resulting in a large chromosome number(2n=130).After three WGDs,okra has undergone extensive genomic deletions and retained substantial numbers of genes related to secondary metabolite biosynthesis and environmental adaptation,resulting in significant differences between okra and G.raimondii in the gene families related to cellulose synthesis.Combining transcriptomic and metabolomic analysis,we revealed the relationship between gene expression and metabolite content change across different okra developmental stages.Furthermore,the sinapic acid/S-lignin biosynthesis-related gene families have experienced remarkable expansion in okra,and the expression of key enzymes involved in the sinapic acid/S-lignin biosynthesis pathway vary greatly across developmental periods,which partially explains the differences in metabolite content across the different stages.Our study gains insights into the comprehensive evolutionary history of Malvaceae species and the genetic basis that underlies the nutrient content changes in okra,which will facilitate the functional study and genetic improvement of okra varieties.
基金supported by the Natural Science Foundation of Sichuan(No.2023NSFSC0683)Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(No:ZYYCXTD-D-202209)the‘Xinglin Scholar’Discipline Talent Research Promotion Program of Chengdu University of TCM(No.MPRC2021036).
文摘Polygala tenuifolia is a perennial medicinal plant that has been widely used in traditional Chinese medicine for treating mental diseases.However,the lack of genomic resources limits the insight into its evolutionary and biological characterization.In the present work,we reported the P.tenuifolia genome,the first genome assembly of the Polygalaceae family.We sequenced and assembled this genome by a combination of Illumnina,PacBio HiFi,and Hi-C mapping.The assembly includes 19 pseudochromosomes covering∼92.68%of the assembled genome(∼769.62 Mb).There are 36463 protein-coding genes annotated in this genome.Detailed comparative genome anal-ysis revealed that P.tenuifolia experienced two rounds of whole genome duplication that occurred∼39–44 and∼18–20 million years ago,respectively.Accordingly,we systematically reconstructed ancestral chromosomes of P.tenuifolia and inferred its chromosome evolution trajectories from the common ancestor of core eudicots to the present species.Based on the transcriptomics data,enzyme genes and transcription factors involved in the synthesis of triterpenoid saponin in P.tenuifolia were identified.Further analysis demonstrated that whole-genome duplications and tandem duplications play critical roles in the expansion of P450 and UGT gene families,which contributed to the synthesis of triterpenoid saponins.The genome and transcriptome data will not only provide valuable resources for comparative and functional genomic researches on Polygalaceae,but also shed light on the synthesis of triterpenoid saponin.
基金supported by the National Key R&D Program of China(No.2019YFD1000200)the National Nature Science Foundation of China(No.32102321)+2 种基金the Chongqing Science and Technology Commission(cstc2021jcyj-msxmX1156 and cstc2021jscxgksbX0010)the Innovation Research Group Funds for Chongqing Universities(CXQT19005)the Fundamental Research Funds for the Central Universities(SWU-KT22055).
文摘Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.
文摘How organizations analyze and use data for decision-making has been changed by cognitive computing and artificial intelligence (AI). Cognitive computing solutions can translate enormous amounts of data into valuable insights by utilizing the power of cutting-edge algorithms and machine learning, empowering enterprises to make deft decisions quickly and efficiently. This article explores the idea of cognitive computing and AI in decision-making, emphasizing its function in converting unvalued data into valuable knowledge. It details the advantages of utilizing these technologies, such as greater productivity, accuracy, and efficiency. Businesses may use cognitive computing and AI to their advantage to obtain a competitive edge in today’s data-driven world by knowing their capabilities and possibilities [1].
文摘Lipid droplets (LDs) are ubiquitous cellular orga nelles that perform functions mostly dedicated to energy homeostasis and lipid metabolism.As neutral lipid depots (triacylglycerol,sterol esters),they can be rapidly mobilized through lipase-mediated hydrolysis (lipolysis) or via lipophagy,a specific form of autophagy devoted to consumption of LDs inside the lysosome.
