Well spread pachytene bivalents and high resolution G-handing patterns have been obtainedby using the prolonged hypotonic treatment combined with the trypsin-hot salt banding technique in the sper-maries of rice field...Well spread pachytene bivalents and high resolution G-handing patterns have been obtainedby using the prolonged hypotonic treatment combined with the trypsin-hot salt banding technique in the sper-maries of rice field eels(Monopterus albus Zuiew).Each bivalent has been idendified and a total of 310—450 bands on 12 bivalents observed.This might bethe largest number of multiple bands obtained from fish chromosomes so far published.An idiogram of 372G-bands has been set up and detailed descriptions of the landmark systems and the band positions have beengiven.展开更多
Well spread pachytene bivalents with high-resolution multiple bands of zebrafish were obtained after the testes were treated with alkaline hypotonic solution and high chloroform fixative solution. This might be the pa...Well spread pachytene bivalents with high-resolution multiple bands of zebrafish were obtained after the testes were treated with alkaline hypotonic solution and high chloroform fixative solution. This might be the pattern with the largest number of multiple bands obtained from fish chromosomes so far published. Both the number and character of the bands in each bivalent were stable. According to the principles of ISCN (1978) and ISCN (1981), an idiogram of 599 bands was set up, and the detailed description of the landmark system and the band positions were given.展开更多
基金the National Doctorate Research Foundation of Chinathe National Natural Science Foundation of China
文摘Well spread pachytene bivalents and high resolution G-handing patterns have been obtainedby using the prolonged hypotonic treatment combined with the trypsin-hot salt banding technique in the sper-maries of rice field eels(Monopterus albus Zuiew).Each bivalent has been idendified and a total of 310—450 bands on 12 bivalents observed.This might bethe largest number of multiple bands obtained from fish chromosomes so far published.An idiogram of 372G-bands has been set up and detailed descriptions of the landmark systems and the band positions have beengiven.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos.30170508 & 39970406) the Research Fund for the Doctoral Program Foundation of Higher Education (Grant No. 98048628).
文摘Well spread pachytene bivalents with high-resolution multiple bands of zebrafish were obtained after the testes were treated with alkaline hypotonic solution and high chloroform fixative solution. This might be the pattern with the largest number of multiple bands obtained from fish chromosomes so far published. Both the number and character of the bands in each bivalent were stable. According to the principles of ISCN (1978) and ISCN (1981), an idiogram of 599 bands was set up, and the detailed description of the landmark system and the band positions were given.
