Objective To study the clinical epidemiology of ossification of ligamentum flavum(OLF)and evaluate its characteristics of prevalence, morphology, and distribution in a common population.Some factors associated with it...Objective To study the clinical epidemiology of ossification of ligamentum flavum(OLF)and evaluate its characteristics of prevalence, morphology, and distribution in a common population.Some factors associated with its occurrence were analyzed展开更多
Objective To study the microscopic changes of white matter and the relationship between white matter changes and cognitive impairment in Alzheimer’s disease(AD)using voxel-based analysis of DTI.Methods Thirty-seven p...Objective To study the microscopic changes of white matter and the relationship between white matter changes and cognitive impairment in Alzheimer’s disease(AD)using voxel-based analysis of DTI.Methods Thirty-seven patients with probable AD,and 32 normal controls(NC)were all examined by MMSE scores,and un-展开更多
Background The well-known <pyrotherapy, of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play...Background The well-known <pyrotherapy, of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play a pivotal role in neuroinflammatory processes. Maternal viral infection during pregnancy is associated with an increased risk for psychiatric disorders with presumed neurodevelopmental origin, including autism spectrum disorders and schizophrenia. The present study was to quantify microglia activation in vivo in the mature offspring of rats exposed to polyriboinosinic-polyribocytidilicacid(Poly l:C) during pregnancy using 11 C'PK11195 positron emission tomography(PET) and immunohistochemistry.Objective The study aimed to quantify microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly l:C exposed rats.Methods Offspring of Poly l:C-treated dams were the model group, offspring of saline-treated dams were the control group. Behavioural test for two groups was taken by spontaneous activity, prepulse inhibition(PPI) and latent inhibition(LI) test(including active avoidance conditioning task and passive avoidance conditioning task). Randomly selected successful model rats were assessed by behavioural test in the model group and control group rats.11 C-PK11195 micro-PET/CT and immunohistochemistry were performed on the selected rats to measure microglia activation.Results The treatment group showed hyperlocomotion and deficits in PPI and LI compared with the control group. The treatment group also showed an increased11 C-PK11195 uptake ratio in the prefrontal cortex(f=-3.990, p=0.003) and hippocampus(f=-4.462,p=0.001). The number of activated microglia cells was significantly higher in the treatment group than in the control gro叩(hippocampus: f=8.204, p<0.001; prefrontal:f=6.995, p<0.001). Within the treatment group, there were significant correlations between the behavioural parameters and the activation of microglia as measured by PET and immunohistochemistry.Conclusions The present study demonstrated microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly l:C exposed rats.This study suggests that microglia activation may play a possible or potential role in the pathogenesis of schizophrenia.展开更多
BACKGROUND Tracheoesophageal fistula(TEF)is a congenital anomaly characterized by interruptions in esophageal continuity with or without fistulous communication to the trachea.Anesthetic management during TEF repair i...BACKGROUND Tracheoesophageal fistula(TEF)is a congenital anomaly characterized by interruptions in esophageal continuity with or without fistulous communication to the trachea.Anesthetic management during TEF repair is challenging because of the difficulty of perioperative airway management.It is important to determine the appropriate position of the endotracheal tube(ETT)for proper ventilation and to prevent excessive gastric dilatation.Therefore,the tip of the ETT should be placed immediately below the fistula and above the carina.CASE SUMMARY A full-term,one-day-old,2.4 kg,50 cm male neonate was diagnosed with TEF type C.During induction,an ETT was inserted using video laryngoscope and advanced deeply to ensure that the tip passed over the fistula,according to known strategies.The passage of the ETT through the vocal cords was confirmed via video laryngoscope.However,after inflating the ETT cuff,breath sounds were not heard on bilateral lung auscultation.Instead,gastric sounds were heard.Considering that a large fistula(approximately 6.60 mm×4.54 mm)located 10.2mm above the carina was confirmed on preoperative tracheal computed tomography,the possibility of unintentional esophageal intubation was highly suspected.Therefore,we decided to uncuff and withdraw the ETT carefully for repositioning,while monitoring auscultation and end-tidal CO2 simultaneously.At a certain point(9.5 cm from the lip),clear breath sounds and proper end-tidal CO_(2) readings were suddenly achieved,and adequate ventilation was possible.CONCLUSION Preanesthetic anatomical evaluation with imaging studies in TEF is necessary to minimize complications related to airway management.展开更多
We develop an improved region growing method to realize automatic retinal pigment epithelium(RPE) cell segmentation for photoacoustic microscopy(PAM) imaging. The minimum bounding rectangle of the segmented region is ...We develop an improved region growing method to realize automatic retinal pigment epithelium(RPE) cell segmentation for photoacoustic microscopy(PAM) imaging. The minimum bounding rectangle of the segmented region is used in this method to dynamically update the growing threshold for optimal segmentation. Phantom images and PAM imaging results of normal porcine RPE are applied to demonstrate the effectiveness of the segmentation. The method realizes accurate segmentation of RPE cells and also provides the basis for quantitative analysis of cell features such as cell area and component content, which can have potential applications in studying RPE cell functions for PAM imaging.展开更多
Objective: TO evaluate the clinical value of MR multi-imaging technique in diagnosing and assessing the resectability of pancreato-biliary tumor. Methods The prospective diagnosis and resectability of 17 patients with...Objective: TO evaluate the clinical value of MR multi-imaging technique in diagnosing and assessing the resectability of pancreato-biliary tumor. Methods The prospective diagnosis and resectability of 17 patients with suspicious pancreato-biliary tumors were evaluated. Surgical findings and pathologic results confirmed pancreatic adenocarcinoma in 11 cases, cholangiocarcinoma in 4, and non-neoplastic lesion in 2. MR multi-imaging protocol, including MR cross-sectional imaging, us cholangiopancreatography (MRCP ), and three-dimensional dynamic contrast-enhanced MR portography (3D DCE MRP), were performed in all patients. Results MR multi-imaging technique allowed-correct diagnosing 15 of 17 (88. 2% ) patients with pancreato-biliary tumors. The accuracy in detecting the range of tumor invasion was 64. 4%. The sensitivity, speificity, accuracy, positive, and negative predictive value of MR multi-imaging technique in assessing the resectability of pancreato-biliary tumors were 83. 3%, 77. 8%, 80. 0%, 71. 4%, and 87. 5%, respectively. Conclusion MR multi-imaging technique can not only improve the diagnostic ability of pancreato-biliary tumor, but also assess the surgical reartability of the tumor. With the fast development of MR techniques, the diagnosing and pre-operative assessment of aoncreato-biliary tumor may be more simplified and efficient by using the non-invasive "all-in-one" method.展开更多
文摘Objective To study the clinical epidemiology of ossification of ligamentum flavum(OLF)and evaluate its characteristics of prevalence, morphology, and distribution in a common population.Some factors associated with its occurrence were analyzed
文摘Objective To study the microscopic changes of white matter and the relationship between white matter changes and cognitive impairment in Alzheimer’s disease(AD)using voxel-based analysis of DTI.Methods Thirty-seven patients with probable AD,and 32 normal controls(NC)were all examined by MMSE scores,and un-
基金provided by the National Natural Science Foundation of China(No 81571318 to XQSNo 81371472 to LXL+5 种基金No 81401110 to XL)the Science and Technology Planning Project of Health and Family Planning Commission(No 201501015 to XQS)the International Science and Technology Cooperation Program of Henan(No 162102410061 to XQS)the Henan Province Union Fund Project(162300410275)the Zhengzhou University doctor team projectthe Youth Fund of the First Affiliated Hospital of Zhengzhou University(to XL and LJP)
文摘Background The well-known <pyrotherapy, of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play a pivotal role in neuroinflammatory processes. Maternal viral infection during pregnancy is associated with an increased risk for psychiatric disorders with presumed neurodevelopmental origin, including autism spectrum disorders and schizophrenia. The present study was to quantify microglia activation in vivo in the mature offspring of rats exposed to polyriboinosinic-polyribocytidilicacid(Poly l:C) during pregnancy using 11 C'PK11195 positron emission tomography(PET) and immunohistochemistry.Objective The study aimed to quantify microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly l:C exposed rats.Methods Offspring of Poly l:C-treated dams were the model group, offspring of saline-treated dams were the control group. Behavioural test for two groups was taken by spontaneous activity, prepulse inhibition(PPI) and latent inhibition(LI) test(including active avoidance conditioning task and passive avoidance conditioning task). Randomly selected successful model rats were assessed by behavioural test in the model group and control group rats.11 C-PK11195 micro-PET/CT and immunohistochemistry were performed on the selected rats to measure microglia activation.Results The treatment group showed hyperlocomotion and deficits in PPI and LI compared with the control group. The treatment group also showed an increased11 C-PK11195 uptake ratio in the prefrontal cortex(f=-3.990, p=0.003) and hippocampus(f=-4.462,p=0.001). The number of activated microglia cells was significantly higher in the treatment group than in the control gro叩(hippocampus: f=8.204, p<0.001; prefrontal:f=6.995, p<0.001). Within the treatment group, there were significant correlations between the behavioural parameters and the activation of microglia as measured by PET and immunohistochemistry.Conclusions The present study demonstrated microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly l:C exposed rats.This study suggests that microglia activation may play a possible or potential role in the pathogenesis of schizophrenia.
文摘BACKGROUND Tracheoesophageal fistula(TEF)is a congenital anomaly characterized by interruptions in esophageal continuity with or without fistulous communication to the trachea.Anesthetic management during TEF repair is challenging because of the difficulty of perioperative airway management.It is important to determine the appropriate position of the endotracheal tube(ETT)for proper ventilation and to prevent excessive gastric dilatation.Therefore,the tip of the ETT should be placed immediately below the fistula and above the carina.CASE SUMMARY A full-term,one-day-old,2.4 kg,50 cm male neonate was diagnosed with TEF type C.During induction,an ETT was inserted using video laryngoscope and advanced deeply to ensure that the tip passed over the fistula,according to known strategies.The passage of the ETT through the vocal cords was confirmed via video laryngoscope.However,after inflating the ETT cuff,breath sounds were not heard on bilateral lung auscultation.Instead,gastric sounds were heard.Considering that a large fistula(approximately 6.60 mm×4.54 mm)located 10.2mm above the carina was confirmed on preoperative tracheal computed tomography,the possibility of unintentional esophageal intubation was highly suspected.Therefore,we decided to uncuff and withdraw the ETT carefully for repositioning,while monitoring auscultation and end-tidal CO2 simultaneously.At a certain point(9.5 cm from the lip),clear breath sounds and proper end-tidal CO_(2) readings were suddenly achieved,and adequate ventilation was possible.CONCLUSION Preanesthetic anatomical evaluation with imaging studies in TEF is necessary to minimize complications related to airway management.
基金supported by the National Natural Science Foundation of China(NSFC)(Nos.81171377,61273368,61472247,61307015,and 61675134)the Open Research Fund of State Key Laboratory of Transient Optics and Photonics,Chinese Academy of Sciences(No.SKLST201501)
文摘We develop an improved region growing method to realize automatic retinal pigment epithelium(RPE) cell segmentation for photoacoustic microscopy(PAM) imaging. The minimum bounding rectangle of the segmented region is used in this method to dynamically update the growing threshold for optimal segmentation. Phantom images and PAM imaging results of normal porcine RPE are applied to demonstrate the effectiveness of the segmentation. The method realizes accurate segmentation of RPE cells and also provides the basis for quantitative analysis of cell features such as cell area and component content, which can have potential applications in studying RPE cell functions for PAM imaging.
文摘Objective: TO evaluate the clinical value of MR multi-imaging technique in diagnosing and assessing the resectability of pancreato-biliary tumor. Methods The prospective diagnosis and resectability of 17 patients with suspicious pancreato-biliary tumors were evaluated. Surgical findings and pathologic results confirmed pancreatic adenocarcinoma in 11 cases, cholangiocarcinoma in 4, and non-neoplastic lesion in 2. MR multi-imaging protocol, including MR cross-sectional imaging, us cholangiopancreatography (MRCP ), and three-dimensional dynamic contrast-enhanced MR portography (3D DCE MRP), were performed in all patients. Results MR multi-imaging technique allowed-correct diagnosing 15 of 17 (88. 2% ) patients with pancreato-biliary tumors. The accuracy in detecting the range of tumor invasion was 64. 4%. The sensitivity, speificity, accuracy, positive, and negative predictive value of MR multi-imaging technique in assessing the resectability of pancreato-biliary tumors were 83. 3%, 77. 8%, 80. 0%, 71. 4%, and 87. 5%, respectively. Conclusion MR multi-imaging technique can not only improve the diagnostic ability of pancreato-biliary tumor, but also assess the surgical reartability of the tumor. With the fast development of MR techniques, the diagnosing and pre-operative assessment of aoncreato-biliary tumor may be more simplified and efficient by using the non-invasive "all-in-one" method.
