Antibiotics,as widely used antibacterial drug,exist in various environmental media.Antibiotic residues can affect biological metabolism and lead to bacterial resistance and the formation of antibiotic-resistance genes...Antibiotics,as widely used antibacterial drug,exist in various environmental media.Antibiotic residues can affect biological metabolism and lead to bacterial resistance and the formation of antibiotic-resistance genes,posing a threat to human health and ecological safety.Establishing efficient detection methods for antibiotics and antibiotic-resistance genes has great environmental significance.Fluorescence detection methods,due to their fast response,high sensitivity and specificity,and low-cost,are widely used in chemical and biological sensing.This review first summarizes the pre-treatment methods for different types of environmental samples,and then focuses on the recent advances of fluorescence methods for the detection of antibiotics and antibiotic-resistance genes.Finally,main challenges and future research directions of fluorescence methods for antibiotic and antibiotic-resistance genes detection are discussed.This review highlights the promising prospect of fluorescence methods in-situ detection and monitoring of antibiotics and antibiotic-resistance genes,and provides guidance for the construction of overall risk assessment system of environmental media.展开更多
基金supported by the National Key R&D Program of China(No.2019YFC1905400)the National Natural Science Foundation of China(No.22006113)。
文摘Antibiotics,as widely used antibacterial drug,exist in various environmental media.Antibiotic residues can affect biological metabolism and lead to bacterial resistance and the formation of antibiotic-resistance genes,posing a threat to human health and ecological safety.Establishing efficient detection methods for antibiotics and antibiotic-resistance genes has great environmental significance.Fluorescence detection methods,due to their fast response,high sensitivity and specificity,and low-cost,are widely used in chemical and biological sensing.This review first summarizes the pre-treatment methods for different types of environmental samples,and then focuses on the recent advances of fluorescence methods for the detection of antibiotics and antibiotic-resistance genes.Finally,main challenges and future research directions of fluorescence methods for antibiotic and antibiotic-resistance genes detection are discussed.This review highlights the promising prospect of fluorescence methods in-situ detection and monitoring of antibiotics and antibiotic-resistance genes,and provides guidance for the construction of overall risk assessment system of environmental media.
