Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and T...Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and Taounate).Methods:Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyI-1-picrylhydrazyl,total antioxidant capacity and iron-reducing capacity.Total phenolic content was measured by FolinCiocalteu reagent.Results:All the extracts showed significant antioxidant activities and contained important levels of phenols.The ethanol extract(0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay,which was probably due to its high content of polyphenols(274.39±6.94) mg gallic acid equivalent/g dry extract.Total flavonoid content was found equal for all extracts.Conclusions:Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants.The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.展开更多
The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and a...The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and adaptive ability of plants to drought in the arid zone, is considered. The results of studies on the introduction into the culture based on indicators of the osmotic pressure of cell juice are presented.展开更多
The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vit...The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.展开更多
Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and m...Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and mechanisms of action of the plant remain unclear. Therefore, the objective of the present study was to investigate the pharmacological effects of the methanolic extract (MEIG) of this plant belonging to compositae family. Anti-diarrheal and antipyretic activities of the extract were examined in rats. Anti-inflammatory and antinociceptive were studied in mice. At the doses of 200 (P in vitro protein anti-denaturation using Bovine serum albumin and anti-platelet aggregation of human blood activity. It was observed that the extract showed greater percentage of inhibition of BSA (P potential platelet aggregation inhibitory activity in adose-dependent manner. The maximum inhibition was observed at the dose 400 μg/ml展开更多
The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and di...The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and different editions of Pharmacopoeia of People’s Republic of China.After textual research,Inulae Flos has been named as"Inulae Flos"since Shennong Bencaojing(Shennong’s Herbal Classic).Its original plant is Inula japonica Thunb.or I.britannica L.,which is easy to multiply and widely distributed.Inulae Flos is used medically with clean and dry capitulum,and there is also honey-burning method.The description of Inulae Flos in Pharmacopoeia of People’s Republic of China,such as nature and flavor,meridian,efficacy and indications,is generally consistent with the records in traditional herbal books.展开更多
The polysaccharides extracted from Inula britannica flower(IBP) exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatograp...The polysaccharides extracted from Inula britannica flower(IBP) exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography(HPLC) and infrared spectroscopy(IR) methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1:1:1.4:2.7:14.6:6.3:7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide(BCG-LPS),not only the blood alanine aminotransferase(ALT) and aspartate aminotransferase(AST) levels decreased significantly(P<0.05),but aslo the liver tissue malondialdehyde(MDA) level decreased significantly(P<0.01).The superoxide dismutase(SOD) level increased significantly(P<0.01).IBP shows significant anti-liver injury and antioxidation activity.展开更多
Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spect...Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spectral methods.展开更多
The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide(SF-CO 2) for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic...The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide(SF-CO 2) for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide.The effects of factors,including extraction temperature,extraction pressure,SF-CO 2 flow rate,flower powder size,and time on the essential oil yield were studied using the single factor experiment.An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield.Adopting the optimum conditions,the maximum yield reached 10.01% at 40°C temperature,30 MPa pressure,60 mesh flower powder size,20 L h-1SF-CO 2 flow rate,and 90 min extraction time.The antifungal activities of I.britannica essential oil using the SF-CO 2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests.Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1concentration of the essential oil.The mycelial growth of Gaeumannomyces graminis var.tritici was completely inhibited.The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%,respectively.In addition,the essential oil can inhibit the spore germination of Fusarium oxysporum f.sp.vasinfectum,Phytophthora capsici,Colletotrichum orbiculare,and Pyricularia grisea,and the corresponding inhibition rates were 98.26,96.54,87.89,and 87.35% respectively.The present study has demonstrated that the essential oil of I.britannica flowers extracted through the SF-CO 2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.展开更多
Isoalantolactone, a major constituent of Inula racemosa (Compositae) was tested for its antimicrobial action against five bacteria, six human and six plant pathogenic fungi. The lactone showed absolute toxicities at 5...Isoalantolactone, a major constituent of Inula racemosa (Compositae) was tested for its antimicrobial action against five bacteria, six human and six plant pathogenic fungi. The lactone showed absolute toxicities at 500 μg/mL against 3 soil borne phytopathogenic fungi (Gaeu-mannomyces graminis var. tritici, Rhizoctonia cerealis and Phytophthora capsici) with the MICs determined to be 100, 100 and 300 μg/mL, respectively. At the MICs, isoalantolac-tone exhibited its fungi.static nature of toxicity. The lowest fungicidal concentrations of the lactone to G. graminis var. tritici, R. cerealis and P. capsici were shown to be 150, 150 and 350 μg/mL, respectively. Moreover, isoalantolactonc displayed weaker antibacterial activities against Bacillus subtilis, Escherichia coli, Pseudomonas fluorecense, Sarcina lentus and Staphylococcus aureus with MICs of 125, 425, 150, 150 and 100 μg/mL, respectively. The investigation disclosed the strong inhibition of isoalantolactonc to the phytopatho-gcnic fungi,展开更多
OBJECTIVE:To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.METHODS:Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2&...OBJECTIVE:To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.METHODS:Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2'-azinobis(3-ethylbenzothiazoline-6-suphonic acid)di-ammonium salt(ABTS),Folin-Ciocalteu and high-performance liquid chromatography(HPLC)methods.The effect against oxidative DNA base damage was determined analyzing oxidative DNA base damage products by GC-MS/MS.RESULTS:The methanol-water extract of Inula helenium root showed good antioxidant capacity[32.20 mg trolox equivalents(TE)/g dried weight(dw)by ABTS method,54.53 mg TE/g dw by chromium reducing antioxidant capacity method]and total phenolic content[29.83 mg gallic acid equivalent(GAE)/g dw].The chlorogenic acid,ferulic acid,rosmarinic acid and caffeic acid were determined in the extract by HPLC.The chlorogenic acid,ferulic acid and Inula helenium root extract were revealed to inhibit the oxidative DNA base damage by GC-MS/MS.CONCLUSION:The studies showed a crucial decrease in the amount of the DNA base damage products when antioxidants were used.The results showed that ferulic acid has better inhibition than chlorogenic acid for DNA oxidation.The methanol-water extract of Inula helenium root was determined to inhibit the oxidative DNA damage.展开更多
Objective:The objective of the study is to combine network pharmacology with high-performance liquid chromatography(HPLC)to screen for quality markers(Q-markers)of Inulae Flos and predict mechanism on anti-hepatitis.M...Objective:The objective of the study is to combine network pharmacology with high-performance liquid chromatography(HPLC)to screen for quality markers(Q-markers)of Inulae Flos and predict mechanism on anti-hepatitis.Materials and Methods:Active ingredient library of Inulae Flos is structured using databases and the literature.“Compound-target-pathway”network on anti-hepatitis and protein–protein interaction(PPI)network are constructed using network pharmacology.Next,chromatographic fingerprints of Inulae Flos in 7 origins are obtained through HPLC,and chemometric analysis is implemented to identify chemical markers,which is combined with network pharmacology to identify Q-markers and detect content.Results:1,6-O,O-Diacetylbritannilactone,Ivangustin,and Inulanolide A are key ingredients of Inulae Flos to interact with 82 potential targets related to anti-hepatitis.Furthermore,signal transducer and activator of transcription 3,tumor necrosis factor,interleukin-6,and transcription factor AP-1 are the core targets in the PPI network.Chromatographic fingerprints of the Inulae Flos define 20 common peaks and identify 8 peaks using reference substances.Through partial least square discriminant analysis,7compounds including caffeic acid,chlorogenic acid,and 1,6-O,O-Diacetylbritannilactone were main chemical markers for variability.1,6-O,O-Diacetylbritannilactone is both a key ingredient and exclusive chemical marker.Therefore,1,6-O,O-diacetylbritannilactone is a Q-marker of Inulae Flos,and the average content is 1.82 mg/g.Conclusion:1,6-O,O-diacetylbritannilactone is determined to be a Q-marker of Inulae Flos.展开更多
Objective: To observe supplemented inula--Ochrae Decoction (SIOD) in preventing andtreating nausea and vomiting induced bychemotherapy for patients with malignant tumour. Methods: Seventy--two patients weredivided int...Objective: To observe supplemented inula--Ochrae Decoction (SIOD) in preventing andtreating nausea and vomiting induced bychemotherapy for patients with malignant tumour. Methods: Seventy--two patients weredivided into two groups, the patients in testgroup took SIOD and in control group usingondensetron, and the efficacy of SIOD in preventing and treating chemotherapy inducednausea and vomiting were studied prospectively with self--intersection approach. Results:The effective rate of treating vomiting due tochemotherapy with DDP and without DDP inthe test group was 92. 7 % and 93. 5 % respectively, and was higher than that in the controlgroup 87. 8 % and 87. 1 %, the difference wasinsignificant statistically (P > 0. 05 ). Conclusions f The prescription SIOD could preventand treat effectively chemotherapy inducednausea and vomiting without any toxic andside effects, and is inexpensive with high efficacy for clinical use.展开更多
Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E(1 and 2),together with three known analogs.The structures of t...Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E(1 and 2),together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC_(50) values ranging from 2.1 to 36.3 μM.展开更多
Objective To establish a rapid method for detecting acetylbritannilactone(ABL)by online sweeping-micellar electrokinetic chromatography(MEKC)and to elevate the sensitivity of the detection.Methods The combination of o...Objective To establish a rapid method for detecting acetylbritannilactone(ABL)by online sweeping-micellar electrokinetic chromatography(MEKC)and to elevate the sensitivity of the detection.Methods The combination of online sweeping technique with MEKC was used to determine the content of ABL in the extract of Inula britannica in plasma of rats.Results ABL was completely separated within 15 min in running buffer and sample buffer.The optimal conditions were as follows:on uncoated fused quartz silica capillary,with separation voltage of 23 kV,capillary temperature of 25 oC,and detection wavelength of 195 nm.The regression equations revealed good linear relationships between the peak area and concentration of ABL(r=0.998),with the detection limits of 0.005–0.15 mg/mL.The relative standard deviations of migration time and peak areas for intra-and inter-batch were<2.45%and<2.26%,respectively.The recovery rate of this method was 96.3%–97.2%.Conclusion This method provides some advantages in separation speed,testing sensitivity,and operating convenience,with low sample and reagent consumption.The online sweeping-MEKC is an effective method for pharmacokinetic study and analysis on tracing biological samples.展开更多
基金supported by University Sidi Mohamed Ben Abdellah,Fes,Morocco
文摘Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and Taounate).Methods:Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyI-1-picrylhydrazyl,total antioxidant capacity and iron-reducing capacity.Total phenolic content was measured by FolinCiocalteu reagent.Results:All the extracts showed significant antioxidant activities and contained important levels of phenols.The ethanol extract(0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay,which was probably due to its high content of polyphenols(274.39±6.94) mg gallic acid equivalent/g dry extract.Total flavonoid content was found equal for all extracts.Conclusions:Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants.The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.
文摘The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and adaptive ability of plants to drought in the arid zone, is considered. The results of studies on the introduction into the culture based on indicators of the osmotic pressure of cell juice are presented.
文摘The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.
文摘Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and mechanisms of action of the plant remain unclear. Therefore, the objective of the present study was to investigate the pharmacological effects of the methanolic extract (MEIG) of this plant belonging to compositae family. Anti-diarrheal and antipyretic activities of the extract were examined in rats. Anti-inflammatory and antinociceptive were studied in mice. At the doses of 200 (P in vitro protein anti-denaturation using Bovine serum albumin and anti-platelet aggregation of human blood activity. It was observed that the extract showed greater percentage of inhibition of BSA (P potential platelet aggregation inhibitory activity in adose-dependent manner. The maximum inhibition was observed at the dose 400 μg/ml
文摘The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and different editions of Pharmacopoeia of People’s Republic of China.After textual research,Inulae Flos has been named as"Inulae Flos"since Shennong Bencaojing(Shennong’s Herbal Classic).Its original plant is Inula japonica Thunb.or I.britannica L.,which is easy to multiply and widely distributed.Inulae Flos is used medically with clean and dry capitulum,and there is also honey-burning method.The description of Inulae Flos in Pharmacopoeia of People’s Republic of China,such as nature and flavor,meridian,efficacy and indications,is generally consistent with the records in traditional herbal books.
基金Supported by the Science and Technology Development Program of Jilin Province,China(No.20070917-01)the Technology Development Project of Jilin Province,China(No.201205020)
文摘The polysaccharides extracted from Inula britannica flower(IBP) exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography(HPLC) and infrared spectroscopy(IR) methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1:1:1.4:2.7:14.6:6.3:7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide(BCG-LPS),not only the blood alanine aminotransferase(ALT) and aspartate aminotransferase(AST) levels decreased significantly(P<0.05),but aslo the liver tissue malondialdehyde(MDA) level decreased significantly(P<0.01).The superoxide dismutase(SOD) level increased significantly(P<0.01).IBP shows significant anti-liver injury and antioxidation activity.
文摘Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spectral methods.
基金supported by the Scientific and Technological Key Project of Henan Province, China (082102350006 and 102102310242)the College Young Teachers Projects of Henan Province, China (2010GGJS046)
文摘The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide(SF-CO 2) for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide.The effects of factors,including extraction temperature,extraction pressure,SF-CO 2 flow rate,flower powder size,and time on the essential oil yield were studied using the single factor experiment.An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield.Adopting the optimum conditions,the maximum yield reached 10.01% at 40°C temperature,30 MPa pressure,60 mesh flower powder size,20 L h-1SF-CO 2 flow rate,and 90 min extraction time.The antifungal activities of I.britannica essential oil using the SF-CO 2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests.Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1concentration of the essential oil.The mycelial growth of Gaeumannomyces graminis var.tritici was completely inhibited.The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%,respectively.In addition,the essential oil can inhibit the spore germination of Fusarium oxysporum f.sp.vasinfectum,Phytophthora capsici,Colletotrichum orbiculare,and Pyricularia grisea,and the corresponding inhibition rates were 98.26,96.54,87.89,and 87.35% respectively.The present study has demonstrated that the essential oil of I.britannica flowers extracted through the SF-CO 2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.
基金the National Natural Science Foundation of China (Grant Nos. 39725033 and 39970083) and that for Changhong Liu (Grant No. PD-1999-25).
文摘Isoalantolactone, a major constituent of Inula racemosa (Compositae) was tested for its antimicrobial action against five bacteria, six human and six plant pathogenic fungi. The lactone showed absolute toxicities at 500 μg/mL against 3 soil borne phytopathogenic fungi (Gaeu-mannomyces graminis var. tritici, Rhizoctonia cerealis and Phytophthora capsici) with the MICs determined to be 100, 100 and 300 μg/mL, respectively. At the MICs, isoalantolac-tone exhibited its fungi.static nature of toxicity. The lowest fungicidal concentrations of the lactone to G. graminis var. tritici, R. cerealis and P. capsici were shown to be 150, 150 and 350 μg/mL, respectively. Moreover, isoalantolactonc displayed weaker antibacterial activities against Bacillus subtilis, Escherichia coli, Pseudomonas fluorecense, Sarcina lentus and Staphylococcus aureus with MICs of 125, 425, 150, 150 and 100 μg/mL, respectively. The investigation disclosed the strong inhibition of isoalantolactonc to the phytopatho-gcnic fungi,
基金Supported by a Grant from the Bursa Uludag University Research Foundation(Isolation of Phenolic Materials from Some Natural Specimens and Investigation of Their Effects on DNA Oxidative DamageNo.OUAP(F)-2013/13)。
文摘OBJECTIVE:To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.METHODS:Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2'-azinobis(3-ethylbenzothiazoline-6-suphonic acid)di-ammonium salt(ABTS),Folin-Ciocalteu and high-performance liquid chromatography(HPLC)methods.The effect against oxidative DNA base damage was determined analyzing oxidative DNA base damage products by GC-MS/MS.RESULTS:The methanol-water extract of Inula helenium root showed good antioxidant capacity[32.20 mg trolox equivalents(TE)/g dried weight(dw)by ABTS method,54.53 mg TE/g dw by chromium reducing antioxidant capacity method]and total phenolic content[29.83 mg gallic acid equivalent(GAE)/g dw].The chlorogenic acid,ferulic acid,rosmarinic acid and caffeic acid were determined in the extract by HPLC.The chlorogenic acid,ferulic acid and Inula helenium root extract were revealed to inhibit the oxidative DNA base damage by GC-MS/MS.CONCLUSION:The studies showed a crucial decrease in the amount of the DNA base damage products when antioxidants were used.The results showed that ferulic acid has better inhibition than chlorogenic acid for DNA oxidation.The methanol-water extract of Inula helenium root was determined to inhibit the oxidative DNA damage.
基金sponsored by National Key Research and Development Program of China(2018YFC1707000)the Project of the Industry University Research Cooperation and transformation of scientific and technological achievements in Qixia District of Nanjing。
文摘Objective:The objective of the study is to combine network pharmacology with high-performance liquid chromatography(HPLC)to screen for quality markers(Q-markers)of Inulae Flos and predict mechanism on anti-hepatitis.Materials and Methods:Active ingredient library of Inulae Flos is structured using databases and the literature.“Compound-target-pathway”network on anti-hepatitis and protein–protein interaction(PPI)network are constructed using network pharmacology.Next,chromatographic fingerprints of Inulae Flos in 7 origins are obtained through HPLC,and chemometric analysis is implemented to identify chemical markers,which is combined with network pharmacology to identify Q-markers and detect content.Results:1,6-O,O-Diacetylbritannilactone,Ivangustin,and Inulanolide A are key ingredients of Inulae Flos to interact with 82 potential targets related to anti-hepatitis.Furthermore,signal transducer and activator of transcription 3,tumor necrosis factor,interleukin-6,and transcription factor AP-1 are the core targets in the PPI network.Chromatographic fingerprints of the Inulae Flos define 20 common peaks and identify 8 peaks using reference substances.Through partial least square discriminant analysis,7compounds including caffeic acid,chlorogenic acid,and 1,6-O,O-Diacetylbritannilactone were main chemical markers for variability.1,6-O,O-Diacetylbritannilactone is both a key ingredient and exclusive chemical marker.Therefore,1,6-O,O-diacetylbritannilactone is a Q-marker of Inulae Flos,and the average content is 1.82 mg/g.Conclusion:1,6-O,O-diacetylbritannilactone is determined to be a Q-marker of Inulae Flos.
文摘Objective: To observe supplemented inula--Ochrae Decoction (SIOD) in preventing andtreating nausea and vomiting induced bychemotherapy for patients with malignant tumour. Methods: Seventy--two patients weredivided into two groups, the patients in testgroup took SIOD and in control group usingondensetron, and the efficacy of SIOD in preventing and treating chemotherapy inducednausea and vomiting were studied prospectively with self--intersection approach. Results:The effective rate of treating vomiting due tochemotherapy with DDP and without DDP inthe test group was 92. 7 % and 93. 5 % respectively, and was higher than that in the controlgroup 87. 8 % and 87. 1 %, the difference wasinsignificant statistically (P > 0. 05 ). Conclusions f The prescription SIOD could preventand treat effectively chemotherapy inducednausea and vomiting without any toxic andside effects, and is inexpensive with high efficacy for clinical use.
基金Financial support from the National Science and Technology Major Project"Key New Drug Creation and Manufacturing Program"(Nos.2012ZX09301001-001,2015ZX09103002)the National Natural Science Funds of China(Nos.81302657,81573305,81473112)+3 种基金Youth Innovation Promotion Association CAS,the Ministry of Science and Technology(No.2010DFA30980)the Chinese Academy of Sciences(No.KSZD-EW-Z-004-01)the Shanghai Commission of Science and Technology(No.11DZ1970700,12JC1410300)the Research Fund of University of Macao(MYRG2014-00020-ICMS-QRCM and MYRG2015-00153ICMS-QRCM)
文摘Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E(1 and 2),together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC_(50) values ranging from 2.1 to 36.3 μM.
基金Hebei Province Supporting Natural Science Fund(No.06276470)
文摘Objective To establish a rapid method for detecting acetylbritannilactone(ABL)by online sweeping-micellar electrokinetic chromatography(MEKC)and to elevate the sensitivity of the detection.Methods The combination of online sweeping technique with MEKC was used to determine the content of ABL in the extract of Inula britannica in plasma of rats.Results ABL was completely separated within 15 min in running buffer and sample buffer.The optimal conditions were as follows:on uncoated fused quartz silica capillary,with separation voltage of 23 kV,capillary temperature of 25 oC,and detection wavelength of 195 nm.The regression equations revealed good linear relationships between the peak area and concentration of ABL(r=0.998),with the detection limits of 0.005–0.15 mg/mL.The relative standard deviations of migration time and peak areas for intra-and inter-batch were<2.45%and<2.26%,respectively.The recovery rate of this method was 96.3%–97.2%.Conclusion This method provides some advantages in separation speed,testing sensitivity,and operating convenience,with low sample and reagent consumption.The online sweeping-MEKC is an effective method for pharmacokinetic study and analysis on tracing biological samples.
