Sequence-related amplification polymorphism （SRAP） markers closely linked to stem nematode resistance gene were developed in sweetpotato, lpomoea batatas （L.） Lam. Using bulked segregant analysis （BSA）, 200 SRAP...Sequence-related amplification polymorphism （SRAP） markers closely linked to stem nematode resistance gene were developed in sweetpotato, lpomoea batatas （L.） Lam. Using bulked segregant analysis （BSA）, 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781xsusceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nspl, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.展开更多
用甘薯品种农林17号(中紫)的叶柄分离原生质体,对其在添加各种浓度的萘乙酸和激动素的改良细胞薄层培养基中进行悬浮培养。培养1～2 d 后,原生质体再生细胞壁,培养2～3 d 后,再生细胞发生第一次分裂,持续的细胞分裂形成小愈伤组织。悬...用甘薯品种农林17号(中紫)的叶柄分离原生质体,对其在添加各种浓度的萘乙酸和激动素的改良细胞薄层培养基中进行悬浮培养。培养1～2 d 后,原生质体再生细胞壁,培养2～3 d 后,再生细胞发生第一次分裂,持续的细胞分裂形成小愈伤组织。悬浮培养的结果表明萘乙酸和激动素的适宜浓度分别为2.0～5.0mg/L 和1.0～5.0mg/L。此后,将小愈伤组织转移到MS 固体培养基上培养,小愈伤组织迅速生长。本试验达到根的分化.展开更多
Sweet potato(Ipomoea batatas,Lam.)is an important root vegetable in developing countries.After its domestication in Neotropical America,human migration led to the distribution of the sweet potato plant throughout the ...Sweet potato(Ipomoea batatas,Lam.)is an important root vegetable in developing countries.After its domestication in Neotropical America,human migration led to the distribution of the sweet potato plant throughout the world.Both leaf and storage root are high in compounds of nutritional value.Yet,the storage roots are of particular value due to their significant content of provitamin A(β-carotene).The breeding effort for elite sweet potato lines led to the reduction of genetic diversity and the potential to improve other traits.The focus of the present study was to assess the metabolic diversity of 27 sweet potato cultivars including landraces and improved varieties.A metabolite profiling approach was optimised for sweet potato leaf and storage root tissue and 130 metabolites identified with three different analysis platforms.The data highlighted a lack of correlation between storage root phenotype and leaf metabolism.Furthermore,the metabolic diversity of storage roots was based on the secondary metabolism,including phenylpropanoids and carotenoids.Three cultivars of three different flesh colouration(yellow,orange and purple)showed a significant difference of the primary metabolism.This data demonstrates the value of metabolite profiling to breeding programs as a means of identifying differences in phenotypes/chemotypes and characterising parental material for future pre-breeding resources.展开更多
The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variat...The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variations have not been investigated. This study showed that KT1 exhibited significantly higher drought tolerance compared to the cultivated parent Kokei No. 14. The content of proline and activities of superoxide dismutase （SOD） and photosynthesis were significantly increased, while malonaldehyde （MDA） content was significantly decreased compared to Kokei No. 14 under drought stress. KT1 also showed higher expression level of well-known drought stress-responsive genes compared to Kokei No. 14 under drought stress. Amplified fragment length polymorphism （AFLP） and methylation-sensitive amplified polymorphism （MSAP） analyses indicated that KT1 had AFLP and MSAP band patterns consisting of both parent specific bands and changed bands. Fur- ther analysis demonstrated that in KT1. the proportions of Kokei No. 14 specific genome components and methylation sites were much greater than those of I. triloba. KT1 had the same chloroplast and mitochondrial genomes as Kokei No. 14. These results will aid in developing the useful genes ofI. triloba and understanding the evolution and phylogeny of the cultivated sweetpotato.展开更多
Objective: To determine antioxidant activity and phytochemical content from various tubers extracts of sweet potato(Ipomoea batatas) and to explore the correlation of phytochemical content with their antioxidant activ...Objective: To determine antioxidant activity and phytochemical content from various tubers extracts of sweet potato(Ipomoea batatas) and to explore the correlation of phytochemical content with their antioxidant activities. Methods: Antioxidant activities were tested using DPPH and FRAP assays. Total phenolic was calculated by Folin-Ciocalteu reagent, flavonoid content by Chang's method and correlation with their antioxidant activities were analyzed by Pearson's method. Results: PO2 showed highest antioxidant activity, which had the lowest IC50 DPPH(10.54 μg/m L) and the lowest EC50 FRAP(11.14 μg/m L). PO2 showed the highest total phenolic(11.91 g GAE/100 g) and total flavonoid content(17.83 g QE/100 g). There were significantly negative correlation between total phenolic content and flavonoid content in sample PO with their IC50 DPPH and EC50 FRAP. IC50 DPPH of sample PP and PO showed significantly positive correlation with their EC50 FRAP. Conclusions: Result of DPPH method shows that all different ethyl acetate and ethanolic tubers extracts of four varieties of sweet potato are classified as strong and very strong antioxidant. Result of DPPH and FRAP methods indicates that phenolic and flavonoid compounds in sample PO contributes together to antioxidant activities. Antioxidant activities of sample PP and PO by DPPH method are linear to FRAP method.展开更多
Objective:To investigate antinociceptive and anti-inflammatory activities of crude extract from Ipomoea involucrata leaves(Convolvulaceae) in mice and rats.Methods:The antinociceptive activity was tested using acetic ...Objective:To investigate antinociceptive and anti-inflammatory activities of crude extract from Ipomoea involucrata leaves(Convolvulaceae) in mice and rats.Methods:The antinociceptive activity was tested using acetic acid-induced abdominal writhing test in mice.The antiinflammatory activity was evaluated using egg albumin -induced oedema of rat paw.Results: Phytochemical screening showed the presence of alkaloids,flavonoids,saponins,terpenoids and tannin.At the doses of 25-100 mg/kg,Ipomoea involucrata exhibited dose-dependent and significant increase in pain threshold in acetic acid -induced writhing test of mice (P【0.05,student t-test) The administration of Ipomoea involucrata leaf extract(25-100 mg/ kg) showed dose-dependent decreases in paw volume of egg albumin induced oedema in rats and a significant higher anti-inflammatory activity compared to the standard control(Aspirin). Conclusions:These results support the claims on the traditional use of the of Ipomoea involucrata leaves in the treatment of toothache,rheumatic pains and other inflammatory conditions.Studies on the isolation and structural elucidation of the active principle are still needed being carried out.展开更多
基金supported by China Agriculture Research System (CARS-11, Sweetpotato)the National 863 Program of China (2012AA101204)
文摘Sequence-related amplification polymorphism （SRAP） markers closely linked to stem nematode resistance gene were developed in sweetpotato, lpomoea batatas （L.） Lam. Using bulked segregant analysis （BSA）, 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781xsusceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nspl, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.
文摘用甘薯品种农林17号(中紫)的叶柄分离原生质体,对其在添加各种浓度的萘乙酸和激动素的改良细胞薄层培养基中进行悬浮培养。培养1～2 d 后,原生质体再生细胞壁,培养2～3 d 后,再生细胞发生第一次分裂,持续的细胞分裂形成小愈伤组织。悬浮培养的结果表明萘乙酸和激动素的适宜浓度分别为2.0～5.0mg/L 和1.0～5.0mg/L。此后,将小愈伤组织转移到MS 固体培养基上培养,小愈伤组织迅速生长。本试验达到根的分化.
文摘Sweet potato(Ipomoea batatas,Lam.)is an important root vegetable in developing countries.After its domestication in Neotropical America,human migration led to the distribution of the sweet potato plant throughout the world.Both leaf and storage root are high in compounds of nutritional value.Yet,the storage roots are of particular value due to their significant content of provitamin A(β-carotene).The breeding effort for elite sweet potato lines led to the reduction of genetic diversity and the potential to improve other traits.The focus of the present study was to assess the metabolic diversity of 27 sweet potato cultivars including landraces and improved varieties.A metabolite profiling approach was optimised for sweet potato leaf and storage root tissue and 130 metabolites identified with three different analysis platforms.The data highlighted a lack of correlation between storage root phenotype and leaf metabolism.Furthermore,the metabolic diversity of storage roots was based on the secondary metabolism,including phenylpropanoids and carotenoids.Three cultivars of three different flesh colouration(yellow,orange and purple)showed a significant difference of the primary metabolism.This data demonstrates the value of metabolite profiling to breeding programs as a means of identifying differences in phenotypes/chemotypes and characterising parental material for future pre-breeding resources.
基金supported by the China Agriculture Research System(CARS-11,Sweetpotato)the National Natural Science Foundation of China(31461143017)
文摘The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variations have not been investigated. This study showed that KT1 exhibited significantly higher drought tolerance compared to the cultivated parent Kokei No. 14. The content of proline and activities of superoxide dismutase （SOD） and photosynthesis were significantly increased, while malonaldehyde （MDA） content was significantly decreased compared to Kokei No. 14 under drought stress. KT1 also showed higher expression level of well-known drought stress-responsive genes compared to Kokei No. 14 under drought stress. Amplified fragment length polymorphism （AFLP） and methylation-sensitive amplified polymorphism （MSAP） analyses indicated that KT1 had AFLP and MSAP band patterns consisting of both parent specific bands and changed bands. Fur- ther analysis demonstrated that in KT1. the proportions of Kokei No. 14 specific genome components and methylation sites were much greater than those of I. triloba. KT1 had the same chloroplast and mitochondrial genomes as Kokei No. 14. These results will aid in developing the useful genes ofI. triloba and understanding the evolution and phylogeny of the cultivated sweetpotato.
文摘Objective: To determine antioxidant activity and phytochemical content from various tubers extracts of sweet potato(Ipomoea batatas) and to explore the correlation of phytochemical content with their antioxidant activities. Methods: Antioxidant activities were tested using DPPH and FRAP assays. Total phenolic was calculated by Folin-Ciocalteu reagent, flavonoid content by Chang's method and correlation with their antioxidant activities were analyzed by Pearson's method. Results: PO2 showed highest antioxidant activity, which had the lowest IC50 DPPH(10.54 μg/m L) and the lowest EC50 FRAP(11.14 μg/m L). PO2 showed the highest total phenolic(11.91 g GAE/100 g) and total flavonoid content(17.83 g QE/100 g). There were significantly negative correlation between total phenolic content and flavonoid content in sample PO with their IC50 DPPH and EC50 FRAP. IC50 DPPH of sample PP and PO showed significantly positive correlation with their EC50 FRAP. Conclusions: Result of DPPH method shows that all different ethyl acetate and ethanolic tubers extracts of four varieties of sweet potato are classified as strong and very strong antioxidant. Result of DPPH and FRAP methods indicates that phenolic and flavonoid compounds in sample PO contributes together to antioxidant activities. Antioxidant activities of sample PP and PO by DPPH method are linear to FRAP method.
文摘Objective:To investigate antinociceptive and anti-inflammatory activities of crude extract from Ipomoea involucrata leaves(Convolvulaceae) in mice and rats.Methods:The antinociceptive activity was tested using acetic acid-induced abdominal writhing test in mice.The antiinflammatory activity was evaluated using egg albumin -induced oedema of rat paw.Results: Phytochemical screening showed the presence of alkaloids,flavonoids,saponins,terpenoids and tannin.At the doses of 25-100 mg/kg,Ipomoea involucrata exhibited dose-dependent and significant increase in pain threshold in acetic acid -induced writhing test of mice (P【0.05,student t-test) The administration of Ipomoea involucrata leaf extract(25-100 mg/ kg) showed dose-dependent decreases in paw volume of egg albumin induced oedema in rats and a significant higher anti-inflammatory activity compared to the standard control(Aspirin). Conclusions:These results support the claims on the traditional use of the of Ipomoea involucrata leaves in the treatment of toothache,rheumatic pains and other inflammatory conditions.Studies on the isolation and structural elucidation of the active principle are still needed being carried out.
