基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,T...基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)预测核桃活性成分的作用靶点,运用OMIM、GeneCards和DrugBank数据库检索HUA疾病靶点。使用Cytoscape 3.7.2软件进行作用靶点-活性成分-疾病网络绘制,利用Metascape平台进行富集分析。使用AutoDockTools 1.5.6软件进行分子对接,对活性成分及重要靶点的结合度进行验证。结果表明,共筛选出核桃活性成分5种,与HUA有交集靶点16个;富集程度较高的信号通路包括癌症信号通路、化学致癌-受体激活信号通路、糖尿病并发症中的晚期糖基化产物-晚期糖基化终末产物受体信号通路和卡波西肉瘤相关疱疹病毒信号通路;通过分子对接验证试验可知,核桃中主要活性成分与重要靶点结合紧密。展开更多
We systematically evaluated fatty acids and triacylglycerol composition,as well as tocopherol,phytosterol,and phenolics,in walnut oil and compared the cholesterol-lowering effects of oil processed with different metho...We systematically evaluated fatty acids and triacylglycerol composition,as well as tocopherol,phytosterol,and phenolics,in walnut oil and compared the cholesterol-lowering effects of oil processed with different methods(cold pressing,roast-pressing,hexane extraction,subcritical butane extraction,and supercritical CO_(2) extraction).The different methods did not affect the lipid composition of walnut oil.The tocopherol(41.11 mg/100 g)and total phenolic content(TPC,4.26 mg/100 g)of roast-pressed walnut oil and the phytosterol contents of subcritical butane-extracted walnut oil(106.51 mg/100 g)were higher than those of other tested oils.Walnut oil significantly decreased cholesterol synthesis by downregulating the expression of HMGCR,SREBP-2,and CYP51 genes,and increased cholesterol efflux by upregulating the expression of ABCG1,thus significantly reducing total cholesterol and triacylglycerol.Phytosterols and TPC in walnut oil were responsible for lowering cholesterol;the optimal concentration of phytosterols was 10μg/mL,and that of TPC was 12.5×10^(-3)μg/mL.Through process optimization,a new processing method for walnut oil based on biological evaluation was preliminarily established.展开更多
LIM(LIN11,Isl1和MEC3)可参与细胞骨架构建、代谢调控以及生物和非生物胁迫等多种生命过程,是真核生物谱系中普遍存在的重要转录因子之一。为深入了解核桃LIM基因家族信息,本研究从核桃全基因组数据库中最终筛选确定了15个LIM基因,利用...LIM(LIN11,Isl1和MEC3)可参与细胞骨架构建、代谢调控以及生物和非生物胁迫等多种生命过程,是真核生物谱系中普遍存在的重要转录因子之一。为深入了解核桃LIM基因家族信息,本研究从核桃全基因组数据库中最终筛选确定了15个LIM基因,利用生物信息学方法对核桃LIM家族进行系统分析。结果表明,15个核桃LIM基因,其氨基酸序列长度介于189~577 aa,理论等电点介于5.56~9.08,分子量介于20.80~65.85 k D,均为疏水性蛋白,主要位于细胞核和叶绿体中,根据进化分析将其分为5个亚族。启动子区顺式作用元件分析表明,该基因家族成员受脱落酸、乙烯、茉莉酸甲酯和水杨酸4种激素调节,并能响应低温逆境。本研究基于核桃全基因组分析,鉴定出15个LIM基因家族成员,系统分析了理化性质、基因结构、染色体定位及顺式作用元件,为进一步探索核桃LIM基因家族在核桃树体响应逆境胁迫的生物学功能提供了参考依据。展开更多
文摘基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)预测核桃活性成分的作用靶点,运用OMIM、GeneCards和DrugBank数据库检索HUA疾病靶点。使用Cytoscape 3.7.2软件进行作用靶点-活性成分-疾病网络绘制,利用Metascape平台进行富集分析。使用AutoDockTools 1.5.6软件进行分子对接,对活性成分及重要靶点的结合度进行验证。结果表明,共筛选出核桃活性成分5种,与HUA有交集靶点16个;富集程度较高的信号通路包括癌症信号通路、化学致癌-受体激活信号通路、糖尿病并发症中的晚期糖基化产物-晚期糖基化终末产物受体信号通路和卡波西肉瘤相关疱疹病毒信号通路;通过分子对接验证试验可知,核桃中主要活性成分与重要靶点结合紧密。
基金supported by the National Natural Science Foundation of China Youth Science Foundation Project(grant numbers 32001735).
文摘We systematically evaluated fatty acids and triacylglycerol composition,as well as tocopherol,phytosterol,and phenolics,in walnut oil and compared the cholesterol-lowering effects of oil processed with different methods(cold pressing,roast-pressing,hexane extraction,subcritical butane extraction,and supercritical CO_(2) extraction).The different methods did not affect the lipid composition of walnut oil.The tocopherol(41.11 mg/100 g)and total phenolic content(TPC,4.26 mg/100 g)of roast-pressed walnut oil and the phytosterol contents of subcritical butane-extracted walnut oil(106.51 mg/100 g)were higher than those of other tested oils.Walnut oil significantly decreased cholesterol synthesis by downregulating the expression of HMGCR,SREBP-2,and CYP51 genes,and increased cholesterol efflux by upregulating the expression of ABCG1,thus significantly reducing total cholesterol and triacylglycerol.Phytosterols and TPC in walnut oil were responsible for lowering cholesterol;the optimal concentration of phytosterols was 10μg/mL,and that of TPC was 12.5×10^(-3)μg/mL.Through process optimization,a new processing method for walnut oil based on biological evaluation was preliminarily established.
文摘LIM(LIN11,Isl1和MEC3)可参与细胞骨架构建、代谢调控以及生物和非生物胁迫等多种生命过程,是真核生物谱系中普遍存在的重要转录因子之一。为深入了解核桃LIM基因家族信息,本研究从核桃全基因组数据库中最终筛选确定了15个LIM基因,利用生物信息学方法对核桃LIM家族进行系统分析。结果表明,15个核桃LIM基因,其氨基酸序列长度介于189~577 aa,理论等电点介于5.56~9.08,分子量介于20.80~65.85 k D,均为疏水性蛋白,主要位于细胞核和叶绿体中,根据进化分析将其分为5个亚族。启动子区顺式作用元件分析表明,该基因家族成员受脱落酸、乙烯、茉莉酸甲酯和水杨酸4种激素调节,并能响应低温逆境。本研究基于核桃全基因组分析,鉴定出15个LIM基因家族成员,系统分析了理化性质、基因结构、染色体定位及顺式作用元件,为进一步探索核桃LIM基因家族在核桃树体响应逆境胁迫的生物学功能提供了参考依据。
