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烟曲霉极性因子kipA基因的敲除与鉴定
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作者 朱均昊 李莉 +1 位作者 章强强 陈裕充 《中国真菌学杂志》 CSCD 2015年第6期332-335,共4页
目的构建烟曲霉kipA基因突变株。方法通过In-Fusion技术将kipA基因的上下游基因与抗性基因顺序链接,以潮霉素抗性基因替换靶基因,达到用同源重组手段敲除烟曲霉kipA基因的目的,以realtime-PCR对基因突变株进行鉴定和验证。结果 RT-PCR... 目的构建烟曲霉kipA基因突变株。方法通过In-Fusion技术将kipA基因的上下游基因与抗性基因顺序链接,以潮霉素抗性基因替换靶基因,达到用同源重组手段敲除烟曲霉kipA基因的目的,以realtime-PCR对基因突变株进行鉴定和验证。结果 RT-PCR结果显示,成功构建烟曲霉kipA基因突变株。结论烟曲霉kipA基因突变株的构建,为后续烟曲霉极性生长与血管侵袭性的研究提供了实验基础。 展开更多
关键词 烟曲霉 kipa基因 极性生长
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Vaccination Statistics and Reality: How Many Horses Are Really Vaccinated against Equine Influenza?
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作者 Heli I. Koskinen 《Journal of Agricultural Science and Technology(A)》 2014年第5期443-448,共6页
Standard schedules for equine influenza vaccinations have been published in Finland. However, no knowledge about the actual number of vaccinations has been available. Based on previous preliminary studies in slaughter... Standard schedules for equine influenza vaccinations have been published in Finland. However, no knowledge about the actual number of vaccinations has been available. Based on previous preliminary studies in slaughterhouses and among horse owners, it was suspected that vaccination recommendations are not always followed. The aims were to investigate vaccination status against equine influenza among Finnish racehorses in regional competitions. Vaccination status was investigated: (1) by utilizing database vaccination information of show jumping and dressage horses and (2) by carrying out a survey among horse industry experts. A total of 400 horses were selected at the beginning of the Finnish competition season in 2012 and five experts (key persons) were invited to a repeated survey implemented by Delphi techniques. Overall, 50%-100% of horses were properly vaccinated depending on the source available. Thirty-four percent (34%) of the horses had the competition license but no vaccination markings in the database. Differences between types of competition (show jumping and dressage) and between Southern and Northern parts of Finland were found. Vaccinations of show jumping horses were registered more often than vaccinations of dressage horses. Ninety percent (90%) of unvaccinated horses in the database were eight years or older. It can be concluded that equine influenza vaccination registry coverage among racehorses in Finland is incomplete. This poses a risk of misunderstanding, and the growing responsibility of competition organizers. 展开更多
关键词 Competition license Delphi Equestrian Federation of Finland equine influenza vaccine HORSE kipa database.
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