本文优化并建立了饲料中16种霉菌毒素的多残留提取、净化及液相色谱-三重四级杆/线性离子阱串联质谱(Qtrap-LC-MS/MS)的检测方法。样品经20 m L乙腈/水/乙酸(V∶V∶V,70∶29∶1)溶液提取、稀释和上机检测。采用0.1%甲酸的1 mmol/L NH4A...本文优化并建立了饲料中16种霉菌毒素的多残留提取、净化及液相色谱-三重四级杆/线性离子阱串联质谱(Qtrap-LC-MS/MS)的检测方法。样品经20 m L乙腈/水/乙酸(V∶V∶V,70∶29∶1)溶液提取、稀释和上机检测。采用0.1%甲酸的1 mmol/L NH4Ac的水相作为弱洗脱流动相梯度洗脱程序,离子源温度为500℃,采用分段MRM扫描模式,100ms的驻留时间,16种霉菌毒素分离理想、基线平稳、选择离子信号强度高、干扰离子信号弱;同时,得到了与理论计算值非常接近的所有选择离子精确质量数。完成了16种毒素的Qtrap-LC-MS/MS方法条件优化,成功的实现饲料中16种霉菌毒素的快速定性、定量分析。展开更多
A high-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry with linear trap detection method for the quantify and determination of a lot of pesticide residues in vegetable is described. ...A high-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry with linear trap detection method for the quantify and determination of a lot of pesticide residues in vegetable is described. The pesticide were extracted from more vegetable(such as new ginger,garlic,shallot) with acetonitrile and the extract purified by SPE(solid phase extract). To set up EPI(enhance product ion) MS library.LC-MS/MS limit of detection (LOD) based on a signal/noise ratio of 3,was estimated to be 1 μg/kg for all pesticides. The overall average recovery in twenty-five compounds spiked vegetable was 70%-115%. LC-MS/MS-QTRAP has shown itself to be a senitive,selective and rapid method and was successfully used for the quantify and confirmation analysis of more pesticides in more difference vegetable samples for regulatory purposes.展开更多
文摘本文优化并建立了饲料中16种霉菌毒素的多残留提取、净化及液相色谱-三重四级杆/线性离子阱串联质谱(Qtrap-LC-MS/MS)的检测方法。样品经20 m L乙腈/水/乙酸(V∶V∶V,70∶29∶1)溶液提取、稀释和上机检测。采用0.1%甲酸的1 mmol/L NH4Ac的水相作为弱洗脱流动相梯度洗脱程序,离子源温度为500℃,采用分段MRM扫描模式,100ms的驻留时间,16种霉菌毒素分离理想、基线平稳、选择离子信号强度高、干扰离子信号弱;同时,得到了与理论计算值非常接近的所有选择离子精确质量数。完成了16种毒素的Qtrap-LC-MS/MS方法条件优化,成功的实现饲料中16种霉菌毒素的快速定性、定量分析。
文摘A high-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry with linear trap detection method for the quantify and determination of a lot of pesticide residues in vegetable is described. The pesticide were extracted from more vegetable(such as new ginger,garlic,shallot) with acetonitrile and the extract purified by SPE(solid phase extract). To set up EPI(enhance product ion) MS library.LC-MS/MS limit of detection (LOD) based on a signal/noise ratio of 3,was estimated to be 1 μg/kg for all pesticides. The overall average recovery in twenty-five compounds spiked vegetable was 70%-115%. LC-MS/MS-QTRAP has shown itself to be a senitive,selective and rapid method and was successfully used for the quantify and confirmation analysis of more pesticides in more difference vegetable samples for regulatory purposes.
