Battery pack capacity estimation under real-world operating conditions is important for battery performance optimization and health management,contributing to the reliability and longevity of batterypowered systems.Ho...Battery pack capacity estimation under real-world operating conditions is important for battery performance optimization and health management,contributing to the reliability and longevity of batterypowered systems.However,complex operating conditions,coupling cell-to-cell inconsistency,and limited labeled data pose great challenges to accurate and robust battery pack capacity estimation.To address these issues,this paper proposes a hierarchical data-driven framework aimed at enhancing the training of machine learning models with fewer labeled data.Unlike traditional data-driven methods that lack interpretability,the hierarchical data-driven framework unveils the“mechanism”of the black box inside the data-driven framework by splitting the final estimation target into cell-level and pack-level intermediate targets.A generalized feature matrix is devised without requiring all cell voltages,significantly reducing the computational cost and memory resources.The generated intermediate target labels and the corresponding features are hierarchically employed to enhance the training of two machine learning models,effectively alleviating the difficulty of learning the relationship from all features due to fewer labeled data and addressing the dilemma of requiring extensive labeled data for accurate estimation.Using only 10%of degradation data,the proposed framework outperforms the state-of-the-art battery pack capacity estimation methods,achieving mean absolute percentage errors of 0.608%,0.601%,and 1.128%for three battery packs whose degradation load profiles represent real-world operating conditions.Its high accuracy,adaptability,and robustness indicate the potential in different application scenarios,which is promising for reducing laborious and expensive aging experiments at the pack level and facilitating the development of battery technology.展开更多
Effective implementation of the fast labeled multi-Bernoulli(FLMB)filter is addressed for target tracking with interval measurements.Firstly,a sequential Monte Carlo(SMC)implementation of the FLMB filter,SMC-FLMB filt...Effective implementation of the fast labeled multi-Bernoulli(FLMB)filter is addressed for target tracking with interval measurements.Firstly,a sequential Monte Carlo(SMC)implementation of the FLMB filter,SMC-FLMB filter,is derived based on generalized likelihood function weighting.Then,a box particle(BP)implementation of the FLMB filter,BP-FLMB filter,is developed,with a computational complexity reduction of the SMC-FLMB filter.Finally,an improved version of the BP-FLMB filter,improved BP-FLMB(IBP-FLMB)filter,is proposed,improving its estimation accuracy and real-time performance under the conditions of low detection probability and high clutter.Simulation results show that the BP-FLMB filter has a great improvement of the real-time performance than the SMC-FLMB filter,with similar tracking performance.Compared with the BP-FLMB filter,the IBP-FLMB filter has better estimation performance and real-time performance under the conditions of low detection probability and high clutter.展开更多
A lot of combinatorial objects have a natural bialgebra structure. In this paper, we prove that the vector space spanned by labeled simple graphs is a bialgebra with the conjunction product and the unshuffle coproduct...A lot of combinatorial objects have a natural bialgebra structure. In this paper, we prove that the vector space spanned by labeled simple graphs is a bialgebra with the conjunction product and the unshuffle coproduct. In fact, it is a Hopf algebra since it is graded connected. The main conclusions are that the vector space spanned by labeled simple graphs arising from the unshuffle coproduct is a Hopf algebra and that there is a Hopf homomorphism from permutations to label simple graphs.展开更多
In the field of medical images,pixel-level labels are time-consuming and expensive to acquire,while image-level labels are relatively easier to obtain.Therefore,it makes sense to learn more information(knowledge)from ...In the field of medical images,pixel-level labels are time-consuming and expensive to acquire,while image-level labels are relatively easier to obtain.Therefore,it makes sense to learn more information(knowledge)from a small number of hard-to-get pixel-level annotated images to apply to different tasks to maximize their usefulness and save time and training costs.In this paper,using Pixel-Level Labeled Images forMulti-Task Learning(PLDMLT),we focus on grading the severity of fundus images for Diabetic Retinopathy(DR).This is because,for the segmentation task,there is a finely labeled mask,while the severity grading task is without classification labels.To this end,we propose a two-stage multi-label learning weakly supervised algorithm,which generates initial classification pseudo labels in the first stage and visualizes heat maps at all levels of severity using Grad-Cam to further provide medical interpretability for the classification task.A multitask model framework with U-net as the baseline is proposed in the second stage.A label update network is designed to alleviate the gradient balance between the classification and segmentation tasks.Extensive experimental results show that our PLDMLTmethod significantly outperforms other stateof-the-art methods in DR segmentation on two public datasets,achieving up to 98.897%segmentation accuracy.In addition,our method achieves comparable competitiveness with single-task fully supervised learning in the DR severity grading task.展开更多
The accuracy of laser-induced breakdown spectroscopy(LIBS) quantitative method is greatly dependent on the amount of certified standard samples used for training. However, in practical applications, only limited stand...The accuracy of laser-induced breakdown spectroscopy(LIBS) quantitative method is greatly dependent on the amount of certified standard samples used for training. However, in practical applications, only limited standard samples with labeled certified concentrations are available. A novel semi-supervised LIBS quantitative analysis method is proposed, based on co-training regression model with selection of effective unlabeled samples. The main idea of the proposed method is to obtain better regression performance by adding effective unlabeled samples in semisupervised learning. First, effective unlabeled samples are selected according to the testing samples by Euclidean metric. Two original regression models based on least squares support vector machine with different parameters are trained by the labeled samples separately, and then the effective unlabeled samples predicted by the two models are used to enlarge the training dataset based on labeling confidence estimation. The final predictions of the proposed method on the testing samples will be determined by weighted combinations of the predictions of two updated regression models. Chromium concentration analysis experiments of 23 certified standard high-alloy steel samples were carried out, in which 5 samples with labeled concentrations and 11 unlabeled samples were used to train the regression models and the remaining 7 samples were used for testing. With the numbers of effective unlabeled samples increasing, the root mean square error of the proposed method went down from 1.80% to 0.84% and the relative prediction error was reduced from 9.15% to 4.04%.展开更多
The development of fluorescien double labeling method in recent years,has identified the peripheral processes of spinal ganglionic neurons project divergently to the somatic and visceral areas,but the chemical nature ...The development of fluorescien double labeling method in recent years,has identified the peripheral processes of spinal ganglionic neurons project divergently to the somatic and visceral areas,but the chemical nature of these neurons remains unknown.For this reason,we developed a tri-labeled method of combining fluorescein tracing and immunocytochemistry to clarify this problem.展开更多
The monoclonal antibody (MAb) RWS4 specific to membrane-associated antigen of human gastric adenocarcinoma was purified by protein A-Sepharose 4B affinity chromatography and labeled with 131I by chloramine-T method. 1...The monoclonal antibody (MAb) RWS4 specific to membrane-associated antigen of human gastric adenocarcinoma was purified by protein A-Sepharose 4B affinity chromatography and labeled with 131I by chloramine-T method. 131-RWS,, was injected (65 μCi/10μg/0.2 ml, intraperitoneally) into the stomach cancer-bearing nude mice (solid tumor about 1 cm in diameter), and its biodistribution was studied by SPECT and gamma-counter over a peroid of 7 days. A clear image of transplanted tumor was observed on the 4th day, and the image became more clear on the 6th day. After SPECT scanning, the animals were killed on the 3rd to 7th day separately and radioactivity was detected in various organs. The ratios of T/NT were calculated. The results were shown as follows: tumor/blood, was 3.41±0.29 on the 6th day and the tumor/other organs (liver, spleen, stomach, lung, heart, kidney and brain etc.) were】3. The specificity of the 131I-RWS4 was 7.74±0.65.展开更多
It was proved in this paper that there exists a labeled resolvable block design LRB (4,3; v ) if and only if v ≡0 (mod 4) and v ≥8, with 8 possible exceptions. It was also proved that there exists a nearly Kirkman s...It was proved in this paper that there exists a labeled resolvable block design LRB (4,3; v ) if and only if v ≡0 (mod 4) and v ≥8, with 8 possible exceptions. It was also proved that there exists a nearly Kirkman system NKS (2,4; v ) if and only if v ≡0 (mod 12) and v ≥24, except possibly when v =264 or 372.展开更多
Five new nitroxyl spin labeled podophyllotoxins (2-6) have been synthesized.Compounds 2 and 3 have been tested for their anticancer activity in vitro. The results showed that compound 3 is much more potent than the cl...Five new nitroxyl spin labeled podophyllotoxins (2-6) have been synthesized.Compounds 2 and 3 have been tested for their anticancer activity in vitro. The results showed that compound 3 is much more potent than the clinically used etoposide (VP-16) in its inhibition of P388 cells, while compound 2 is not展开更多
Three spin labeled daunomycin derivatives 2-4 were synthesized and their biological activities were tested against mouse leukemia L1210 and human liver cancer BEL-7402 cells in vitro.
The purpose of this study was to use the measurement of the PAEE taken from Actiheart in order to calculate the TEE in Costa Rican schoolchildren, and at the same time, to determine the effectiveness when it is compar...The purpose of this study was to use the measurement of the PAEE taken from Actiheart in order to calculate the TEE in Costa Rican schoolchildren, and at the same time, to determine the effectiveness when it is compared against the TEE obtained by the DLW. A total of sixteen male schoolchildren were measured for their total daily energy expenditure (TEE) with the doubly labeled water (DLW) technique. The TEE obtained by the Actiheart monitor was calculated and validated against the DWL. The TEE was obtained adding the physical activity energy expenditure given by the Actiheart, plus the basal metabolic rate, and the energy cost of growth. The Pearson’s product‐moment correlation coefficient and the paired t‐test sample were measured in order to identify the association of the data and to evidence the differences between both measurements of TEE respectively. The Lin’s concordance correlation coefficient and the Bland-Altman plot evaluated the concordance of both methods. The correlation between the TEE obtained by DLW and the one calculated by Actiheart was r = 0.97, P < 0.001. The paired t-test showed no significant differences between both methods. Lin’s concordance correlation coefficient was Cb = 0.99, classified as almost perfect. The study validated the TEE calculated with Actiheart against the TEE measured by the DLW.展开更多
Spin labeled PNAT10 wn synthesized by solid phase method with Boc strategy Theproduct was characterized by ESR and ToF MS analysis revealing a facile spin lforling of PNAby ln silu solid phase synthesis.
Bovine serum albumin (BSA) was labeled with 9, 10-anthraqinone and the photochemical fluorimetric reactivity of the covalently conjugated 9,10-anthraquinone was remarkably improved.The mechanism for the enhancement in...Bovine serum albumin (BSA) was labeled with 9, 10-anthraqinone and the photochemical fluorimetric reactivity of the covalently conjugated 9,10-anthraquinone was remarkably improved.The mechanism for the enhancement in the photochemical reactivity of conjugated 9,10- anthraquinone with BSA was展开更多
In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 indi...In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 individuals, total 30, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14 and adult at month 1, 2, 6, 12 to 24 were injected with 3H-thymidine a DNA precurson, another 10 groups consisting of 3 individuals, total 30, were injected with 3H-uridine a RNA precursor, and another 10 groups of 30 individuals were injected with 3H-leucine a protein precursor, total 90 individuals. Then, all the animals were sacrificed 1 hr after the injections and the liver tissues, the lung tissues, the kidney tissues, the testis and ovary tissues, the adrenal tissues were taken out, fixed and processed for electron microscopic radioautography. On electron microscopic radioautograms obtained from each animal, ten photographs in respective groups, numbers of mitochondria per cell profile area, numbers of labeled mitochondria per cell and the mitochondrial labeling index (LI) labeled with 3H-thymidine showing DNA synthesis, LI labeled with 3H-uridine showing RNA synthesis, and LI labeled with 3H-leucine showing protein synthesis, in various organs, were counted and the results in various organs in respective aging groups were compared, respectively. From the results, it was demonstrated that the numbers of mitochondria in hepatocytes as well as in 3 zones of the adrenal cortex, the zona glomerulosa, fasciculata and reticularis of mice at various ages increased from fetal day 19 to postnatal month 1 due to development and aging of animals. On the other hand, the numbers of labeled mitochondria and the labeling indices of intramitochondrial DNA syntheses incorporating 3H-thymidine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 ones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. Moreover, some other organs such as the lung and the testis were also review and discussed. From the results, it was shown that the activity of intramitochnodrial DNA synthesis, RNA synthesis, and protein synthesis in hepatocytes, adrenal cortical cells, pulmonary cells and testicular cells in developing and aging mice as well as some other organs increased and decreased due to development and aging.展开更多
In this study the compatible chemical and biological investigations of several N-phenylquinolinium derivatives have been carried out in order to find the most perspective quinolinium structures for the nuclear-chemica...In this study the compatible chemical and biological investigations of several N-phenylquinolinium derivatives have been carried out in order to find the most perspective quinolinium structures for the nuclear-chemical synthesis of tritium labeled biomarkers.展开更多
Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by...Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by using polymerase展开更多
基金supported by the National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[Grant No.52222708]the Natural Science Foundation of Beijing Municipality[Grant No.3212033]。
文摘Battery pack capacity estimation under real-world operating conditions is important for battery performance optimization and health management,contributing to the reliability and longevity of batterypowered systems.However,complex operating conditions,coupling cell-to-cell inconsistency,and limited labeled data pose great challenges to accurate and robust battery pack capacity estimation.To address these issues,this paper proposes a hierarchical data-driven framework aimed at enhancing the training of machine learning models with fewer labeled data.Unlike traditional data-driven methods that lack interpretability,the hierarchical data-driven framework unveils the“mechanism”of the black box inside the data-driven framework by splitting the final estimation target into cell-level and pack-level intermediate targets.A generalized feature matrix is devised without requiring all cell voltages,significantly reducing the computational cost and memory resources.The generated intermediate target labels and the corresponding features are hierarchically employed to enhance the training of two machine learning models,effectively alleviating the difficulty of learning the relationship from all features due to fewer labeled data and addressing the dilemma of requiring extensive labeled data for accurate estimation.Using only 10%of degradation data,the proposed framework outperforms the state-of-the-art battery pack capacity estimation methods,achieving mean absolute percentage errors of 0.608%,0.601%,and 1.128%for three battery packs whose degradation load profiles represent real-world operating conditions.Its high accuracy,adaptability,and robustness indicate the potential in different application scenarios,which is promising for reducing laborious and expensive aging experiments at the pack level and facilitating the development of battery technology.
基金supported by the National Natural Science Foundation of China(61871301)the Postdoctoral Science Foundation of China(2018M633470,2020T130494)the Fundamental Research Funds for the Central Universities(XJS210211).
文摘Effective implementation of the fast labeled multi-Bernoulli(FLMB)filter is addressed for target tracking with interval measurements.Firstly,a sequential Monte Carlo(SMC)implementation of the FLMB filter,SMC-FLMB filter,is derived based on generalized likelihood function weighting.Then,a box particle(BP)implementation of the FLMB filter,BP-FLMB filter,is developed,with a computational complexity reduction of the SMC-FLMB filter.Finally,an improved version of the BP-FLMB filter,improved BP-FLMB(IBP-FLMB)filter,is proposed,improving its estimation accuracy and real-time performance under the conditions of low detection probability and high clutter.Simulation results show that the BP-FLMB filter has a great improvement of the real-time performance than the SMC-FLMB filter,with similar tracking performance.Compared with the BP-FLMB filter,the IBP-FLMB filter has better estimation performance and real-time performance under the conditions of low detection probability and high clutter.
文摘A lot of combinatorial objects have a natural bialgebra structure. In this paper, we prove that the vector space spanned by labeled simple graphs is a bialgebra with the conjunction product and the unshuffle coproduct. In fact, it is a Hopf algebra since it is graded connected. The main conclusions are that the vector space spanned by labeled simple graphs arising from the unshuffle coproduct is a Hopf algebra and that there is a Hopf homomorphism from permutations to label simple graphs.
文摘In the field of medical images,pixel-level labels are time-consuming and expensive to acquire,while image-level labels are relatively easier to obtain.Therefore,it makes sense to learn more information(knowledge)from a small number of hard-to-get pixel-level annotated images to apply to different tasks to maximize their usefulness and save time and training costs.In this paper,using Pixel-Level Labeled Images forMulti-Task Learning(PLDMLT),we focus on grading the severity of fundus images for Diabetic Retinopathy(DR).This is because,for the segmentation task,there is a finely labeled mask,while the severity grading task is without classification labels.To this end,we propose a two-stage multi-label learning weakly supervised algorithm,which generates initial classification pseudo labels in the first stage and visualizes heat maps at all levels of severity using Grad-Cam to further provide medical interpretability for the classification task.A multitask model framework with U-net as the baseline is proposed in the second stage.A label update network is designed to alleviate the gradient balance between the classification and segmentation tasks.Extensive experimental results show that our PLDMLTmethod significantly outperforms other stateof-the-art methods in DR segmentation on two public datasets,achieving up to 98.897%segmentation accuracy.In addition,our method achieves comparable competitiveness with single-task fully supervised learning in the DR severity grading task.
基金supported by National Natural Science Foundation of China (No. 51674032)
文摘The accuracy of laser-induced breakdown spectroscopy(LIBS) quantitative method is greatly dependent on the amount of certified standard samples used for training. However, in practical applications, only limited standard samples with labeled certified concentrations are available. A novel semi-supervised LIBS quantitative analysis method is proposed, based on co-training regression model with selection of effective unlabeled samples. The main idea of the proposed method is to obtain better regression performance by adding effective unlabeled samples in semisupervised learning. First, effective unlabeled samples are selected according to the testing samples by Euclidean metric. Two original regression models based on least squares support vector machine with different parameters are trained by the labeled samples separately, and then the effective unlabeled samples predicted by the two models are used to enlarge the training dataset based on labeling confidence estimation. The final predictions of the proposed method on the testing samples will be determined by weighted combinations of the predictions of two updated regression models. Chromium concentration analysis experiments of 23 certified standard high-alloy steel samples were carried out, in which 5 samples with labeled concentrations and 11 unlabeled samples were used to train the regression models and the remaining 7 samples were used for testing. With the numbers of effective unlabeled samples increasing, the root mean square error of the proposed method went down from 1.80% to 0.84% and the relative prediction error was reduced from 9.15% to 4.04%.
文摘The development of fluorescien double labeling method in recent years,has identified the peripheral processes of spinal ganglionic neurons project divergently to the somatic and visceral areas,but the chemical nature of these neurons remains unknown.For this reason,we developed a tri-labeled method of combining fluorescein tracing and immunocytochemistry to clarify this problem.
文摘The monoclonal antibody (MAb) RWS4 specific to membrane-associated antigen of human gastric adenocarcinoma was purified by protein A-Sepharose 4B affinity chromatography and labeled with 131I by chloramine-T method. 131-RWS,, was injected (65 μCi/10μg/0.2 ml, intraperitoneally) into the stomach cancer-bearing nude mice (solid tumor about 1 cm in diameter), and its biodistribution was studied by SPECT and gamma-counter over a peroid of 7 days. A clear image of transplanted tumor was observed on the 4th day, and the image became more clear on the 6th day. After SPECT scanning, the animals were killed on the 3rd to 7th day separately and radioactivity was detected in various organs. The ratios of T/NT were calculated. The results were shown as follows: tumor/blood, was 3.41±0.29 on the 6th day and the tumor/other organs (liver, spleen, stomach, lung, heart, kidney and brain etc.) were】3. The specificity of the 131I-RWS4 was 7.74±0.65.
文摘It was proved in this paper that there exists a labeled resolvable block design LRB (4,3; v ) if and only if v ≡0 (mod 4) and v ≥8, with 8 possible exceptions. It was also proved that there exists a nearly Kirkman system NKS (2,4; v ) if and only if v ≡0 (mod 12) and v ≥24, except possibly when v =264 or 372.
文摘Five new nitroxyl spin labeled podophyllotoxins (2-6) have been synthesized.Compounds 2 and 3 have been tested for their anticancer activity in vitro. The results showed that compound 3 is much more potent than the clinically used etoposide (VP-16) in its inhibition of P388 cells, while compound 2 is not
文摘Three spin labeled daunomycin derivatives 2-4 were synthesized and their biological activities were tested against mouse leukemia L1210 and human liver cancer BEL-7402 cells in vitro.
文摘The purpose of this study was to use the measurement of the PAEE taken from Actiheart in order to calculate the TEE in Costa Rican schoolchildren, and at the same time, to determine the effectiveness when it is compared against the TEE obtained by the DLW. A total of sixteen male schoolchildren were measured for their total daily energy expenditure (TEE) with the doubly labeled water (DLW) technique. The TEE obtained by the Actiheart monitor was calculated and validated against the DWL. The TEE was obtained adding the physical activity energy expenditure given by the Actiheart, plus the basal metabolic rate, and the energy cost of growth. The Pearson’s product‐moment correlation coefficient and the paired t‐test sample were measured in order to identify the association of the data and to evidence the differences between both measurements of TEE respectively. The Lin’s concordance correlation coefficient and the Bland-Altman plot evaluated the concordance of both methods. The correlation between the TEE obtained by DLW and the one calculated by Actiheart was r = 0.97, P < 0.001. The paired t-test showed no significant differences between both methods. Lin’s concordance correlation coefficient was Cb = 0.99, classified as almost perfect. The study validated the TEE calculated with Actiheart against the TEE measured by the DLW.
文摘Spin labeled PNAT10 wn synthesized by solid phase method with Boc strategy Theproduct was characterized by ESR and ToF MS analysis revealing a facile spin lforling of PNAby ln silu solid phase synthesis.
文摘Bovine serum albumin (BSA) was labeled with 9, 10-anthraqinone and the photochemical fluorimetric reactivity of the covalently conjugated 9,10-anthraquinone was remarkably improved.The mechanism for the enhancement in the photochemical reactivity of conjugated 9,10- anthraquinone with BSA was
文摘In order to study the aging changes of intramitochondrial macromolecular synthesis in various organs of aging animals during the development and aging, 10 groups of developing and aging mice, each consisting of 3 individuals, total 30, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14 and adult at month 1, 2, 6, 12 to 24 were injected with 3H-thymidine a DNA precurson, another 10 groups consisting of 3 individuals, total 30, were injected with 3H-uridine a RNA precursor, and another 10 groups of 30 individuals were injected with 3H-leucine a protein precursor, total 90 individuals. Then, all the animals were sacrificed 1 hr after the injections and the liver tissues, the lung tissues, the kidney tissues, the testis and ovary tissues, the adrenal tissues were taken out, fixed and processed for electron microscopic radioautography. On electron microscopic radioautograms obtained from each animal, ten photographs in respective groups, numbers of mitochondria per cell profile area, numbers of labeled mitochondria per cell and the mitochondrial labeling index (LI) labeled with 3H-thymidine showing DNA synthesis, LI labeled with 3H-uridine showing RNA synthesis, and LI labeled with 3H-leucine showing protein synthesis, in various organs, were counted and the results in various organs in respective aging groups were compared, respectively. From the results, it was demonstrated that the numbers of mitochondria in hepatocytes as well as in 3 zones of the adrenal cortex, the zona glomerulosa, fasciculata and reticularis of mice at various ages increased from fetal day 19 to postnatal month 1 due to development and aging of animals. On the other hand, the numbers of labeled mitochondria and the labeling indices of intramitochondrial DNA syntheses incorporating 3H-thymidine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 zones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. The numbers of labeled mitochondria and the labeling indices of intramitochondrial RNA syntheses incorporating 3H-uridine in hepatocytes and 3 ones of cortical cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. Moreover, some other organs such as the lung and the testis were also review and discussed. From the results, it was shown that the activity of intramitochnodrial DNA synthesis, RNA synthesis, and protein synthesis in hepatocytes, adrenal cortical cells, pulmonary cells and testicular cells in developing and aging mice as well as some other organs increased and decreased due to development and aging.
文摘In this study the compatible chemical and biological investigations of several N-phenylquinolinium derivatives have been carried out in order to find the most perspective quinolinium structures for the nuclear-chemical synthesis of tritium labeled biomarkers.
文摘Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by using polymerase
