[Objective] This study aimed to establish a tissue culture technology for Leucophyta brownii ‘Canal Rocks Form’. [Method] Leucophyta brownii ‘Canal Rocks Form’ stems with axillary buds were selected as explants in...[Objective] This study aimed to establish a tissue culture technology for Leucophyta brownii ‘Canal Rocks Form’. [Method] Leucophyta brownii ‘Canal Rocks Form’ stems with axillary buds were selected as explants in vitro to investigate the effects of different concentrations of 6-BA and NAA on proliferation culture and the effects of IBA, NAA and subculture cycle on rooting culture. [Result] The results showed that Leucophyta brownii ‘Canal Rocks Form’ plantlets need low concentrations of phytohormones and the rooting culture was significantly affected by the subculture cycle; the optimal medium for proliferation culture was MS＋0.5 mg/L 6-BA＋0.05 mg/L NAA＋30 g/L sucrose＋5.5 g/L agar, with a proliferation coefficient of 4.25; the plantlets with a subculture cycle of 28 d were the most suitable for rooting culture, with a rooting rate of 95.9% in 1/2 MS＋0.1 mg/L IBA＋0.05 mg/L NAA＋20 g/L sucrose＋5.5 g/L agar, and the average number and length of roots were 4.69 and 1.68 cm, respectively. [Conclusion] This study laid the foundation for establishing sterile culture system of Leucophyta brownii ‘Canal Rocks Form’.展开更多
基金Supported by Project of Development and Reform Commission of He'nan Province(2060403)~~
文摘[Objective] This study aimed to establish a tissue culture technology for Leucophyta brownii ‘Canal Rocks Form’. [Method] Leucophyta brownii ‘Canal Rocks Form’ stems with axillary buds were selected as explants in vitro to investigate the effects of different concentrations of 6-BA and NAA on proliferation culture and the effects of IBA, NAA and subculture cycle on rooting culture. [Result] The results showed that Leucophyta brownii ‘Canal Rocks Form’ plantlets need low concentrations of phytohormones and the rooting culture was significantly affected by the subculture cycle; the optimal medium for proliferation culture was MS＋0.5 mg/L 6-BA＋0.05 mg/L NAA＋30 g/L sucrose＋5.5 g/L agar, with a proliferation coefficient of 4.25; the plantlets with a subculture cycle of 28 d were the most suitable for rooting culture, with a rooting rate of 95.9% in 1/2 MS＋0.1 mg/L IBA＋0.05 mg/L NAA＋20 g/L sucrose＋5.5 g/L agar, and the average number and length of roots were 4.69 and 1.68 cm, respectively. [Conclusion] This study laid the foundation for establishing sterile culture system of Leucophyta brownii ‘Canal Rocks Form’.
