Central serous chorioretinopathy （CSC） is characterized by a localized accumulation of subretinal fluid and an idiopathic focal leakage from choroidal vessels. The exact pathogenesis of CSC, however, still remains o...Central serous chorioretinopathy （CSC） is characterized by a localized accumulation of subretinal fluid and an idiopathic focal leakage from choroidal vessels. The exact pathogenesis of CSC, however, still remains obscure. In this paper, we hypothesized that CSC may result from a response of choroidal vessels to an acute increase in the environmental light intensity leading to a focal leakage from the choroidal vessels. High levels of glucocorticoids, in our proposed model, may cause persistence rather than initiation of the focal leakage, probably by suppressing the synthesis of collagen and extracellular matrix components and inhibiting fibroblastic activity.展开更多
Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information,...Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large Fo V(field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Icontrast. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Icontrast to resolve the 100μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm3and collect 750 images within 6mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-Ⅱ imaging.展开更多
为探明夜间灯光遥感技术在渔业研究中的应用现状和发展趋势,以四大中文数据库(中国知网、维普、万方、百度学术)与“Web of Science”核心合集为数据源,结合CiteSpace知识图谱方法,对国内外利用夜间灯光遥感进行灯光渔业研究的发文量、...为探明夜间灯光遥感技术在渔业研究中的应用现状和发展趋势,以四大中文数据库(中国知网、维普、万方、百度学术)与“Web of Science”核心合集为数据源,结合CiteSpace知识图谱方法,对国内外利用夜间灯光遥感进行灯光渔业研究的发文量、发文机构、文献共被引及高频关键词等进行分析。结果显示:(1)发文量总体上呈现增长趋势,2013年以后热度明显增加,2018年后中国发文量与世界处于同一水平。(2)关键词聚类与频次分析表明,研究区域主要集中在太平洋、大西洋以及中国近海等海域,研究尺度呈现从大范围到精细化的变化特征;云层检测与影像去噪是关系到渔船提取精度的关键预处理步骤;灯光渔船提取方法朝着自动化、高精度、系统化的方向发展。(3)共被引时区图谱分析显示,研究以2005年和2013年为间隔经历了3个阶段,热点从单一数据支持下的渔船分布监测深化为渔场与海洋环境特征相互关系的研究。(4)机构与国家网络分析结果显示,美国和中国是此方向的主要研究国家。展开更多
文摘Central serous chorioretinopathy （CSC） is characterized by a localized accumulation of subretinal fluid and an idiopathic focal leakage from choroidal vessels. The exact pathogenesis of CSC, however, still remains obscure. In this paper, we hypothesized that CSC may result from a response of choroidal vessels to an acute increase in the environmental light intensity leading to a focal leakage from the choroidal vessels. High levels of glucocorticoids, in our proposed model, may cause persistence rather than initiation of the focal leakage, probably by suppressing the synthesis of collagen and extracellular matrix components and inhibiting fibroblastic activity.
基金Technology Program(KQTD20170810110913065,20200925174735005)National Natural Science Foundation of China(62005116,51720105015)Guangdong Provincial Key Laboratory of Advanced Biomaterials(2022B1212010003).
文摘Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large Fo V(field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Icontrast. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Icontrast to resolve the 100μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm3and collect 750 images within 6mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-Ⅱ imaging.
文摘为探明夜间灯光遥感技术在渔业研究中的应用现状和发展趋势,以四大中文数据库(中国知网、维普、万方、百度学术)与“Web of Science”核心合集为数据源,结合CiteSpace知识图谱方法,对国内外利用夜间灯光遥感进行灯光渔业研究的发文量、发文机构、文献共被引及高频关键词等进行分析。结果显示:(1)发文量总体上呈现增长趋势,2013年以后热度明显增加,2018年后中国发文量与世界处于同一水平。(2)关键词聚类与频次分析表明,研究区域主要集中在太平洋、大西洋以及中国近海等海域,研究尺度呈现从大范围到精细化的变化特征;云层检测与影像去噪是关系到渔船提取精度的关键预处理步骤;灯光渔船提取方法朝着自动化、高精度、系统化的方向发展。(3)共被引时区图谱分析显示,研究以2005年和2013年为间隔经历了3个阶段,热点从单一数据支持下的渔船分布监测深化为渔场与海洋环境特征相互关系的研究。(4)机构与国家网络分析结果显示,美国和中国是此方向的主要研究国家。
