Hepatitis C virus(HCV) is a hepatotrophic virus and a major cause of chronic liver disease,including hepatocellular carcinoma,worldwide. The life cycle of HCV is closely associated with the metabolism of lipids and li...Hepatitis C virus(HCV) is a hepatotrophic virus and a major cause of chronic liver disease,including hepatocellular carcinoma,worldwide. The life cycle of HCV is closely associated with the metabolism of lipids and lipoproteins. The main function of lipoproteins is transporting lipids throughout the body. Triglycerides,free cholesterol,cholesteryl esters,and phospholipids are the major components of the transported lipids. The pathway of HCV assembly and secretion is closely linked to lipoprotein production and secretion,and the infectivity of HCV particles largely depends on the interaction of lipoproteins. Moreover,HCV entry into hepatocytes is strongly influenced by lipoproteins. The key lipoprotein molecules mediating these interactions are apolipoproteins. Apolipoproteins are amphipathic proteins on the surface of a lipoprotein particle,which help stabilize lipoprotein structure. They perform a key role in lipoprotein metabolism by serving as receptor ligands,enzyme co-factors,and lipid transport carriers. Understanding the association between the life cycle of HCV and lipoprotein metabolism is important because each step of the life cycle of HCV that is associated with lipoprotein metabolism is a potential target for anti-HCV therapy. In this article,we first concisely review the nature of lipoprotein and its metabolism to better understand the complicated interaction of HCV with lipoprotein. Then,we review the outline of the processes of HCV assembly,secretion,and entry into hepatocytes,focusing on the association with lipoproteins. Finally,we discuss the clinical aspects of disturbed lipid/lipoprotein metabolism and the significance of dyslipoproteinemia in chronic HCV infection with regard to abnormal apolipoproteins.展开更多
目的:研究Lipo-PGE1注射液对无心跳兔肺缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:将16只健康新西兰大白兔随机平均分为2组:对照组以低钾右旋糖酐(LPD)液进行肺灌洗及保存;试验组采用Lipo-PGE1注射液(20μg.L-1)加LPD液灌...目的:研究Lipo-PGE1注射液对无心跳兔肺缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:将16只健康新西兰大白兔随机平均分为2组:对照组以低钾右旋糖酐(LPD)液进行肺灌洗及保存;试验组采用Lipo-PGE1注射液(20μg.L-1)加LPD液灌注(进行肺灌洗及保存)。两组都在4℃保存2 h后再灌注1 h,测定经肺氧合后动脉血氧分压值(PaO2)和肺气道峰压值(PawP)。于再灌注结束后取右上肺组织,测其湿重(Wr)与干重(Wd)、计算湿/干重比(Wr/Wd);ELISA法检测超氧化物歧化酶(SOD)、丙二醛(MDA)含量;免疫组化法检测NF-κB的表达;并用光学显微镜和透射电子显微镜观察再灌注后肺组织结构的病理变化。结果:再灌注30 m in后,两组的PaO2逐渐降低,PawP逐渐升高,实验组PaO2降低程度及PawP的升高程度均低于对照组(P<0.01);实验组的SOD明显高于对照组,MDA及肺组织的NF-κB的表达低于对照组(P<0.01),肺组织的病理变化较对照组轻。结论:Lipo-PGE1注射液能减轻无心跳兔肺缺血/再灌注损伤,改善肺功能,具有肺保护功能。展开更多
文摘Hepatitis C virus(HCV) is a hepatotrophic virus and a major cause of chronic liver disease,including hepatocellular carcinoma,worldwide. The life cycle of HCV is closely associated with the metabolism of lipids and lipoproteins. The main function of lipoproteins is transporting lipids throughout the body. Triglycerides,free cholesterol,cholesteryl esters,and phospholipids are the major components of the transported lipids. The pathway of HCV assembly and secretion is closely linked to lipoprotein production and secretion,and the infectivity of HCV particles largely depends on the interaction of lipoproteins. Moreover,HCV entry into hepatocytes is strongly influenced by lipoproteins. The key lipoprotein molecules mediating these interactions are apolipoproteins. Apolipoproteins are amphipathic proteins on the surface of a lipoprotein particle,which help stabilize lipoprotein structure. They perform a key role in lipoprotein metabolism by serving as receptor ligands,enzyme co-factors,and lipid transport carriers. Understanding the association between the life cycle of HCV and lipoprotein metabolism is important because each step of the life cycle of HCV that is associated with lipoprotein metabolism is a potential target for anti-HCV therapy. In this article,we first concisely review the nature of lipoprotein and its metabolism to better understand the complicated interaction of HCV with lipoprotein. Then,we review the outline of the processes of HCV assembly,secretion,and entry into hepatocytes,focusing on the association with lipoproteins. Finally,we discuss the clinical aspects of disturbed lipid/lipoprotein metabolism and the significance of dyslipoproteinemia in chronic HCV infection with regard to abnormal apolipoproteins.
文摘目的:研究Lipo-PGE1注射液对无心跳兔肺缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:将16只健康新西兰大白兔随机平均分为2组:对照组以低钾右旋糖酐(LPD)液进行肺灌洗及保存;试验组采用Lipo-PGE1注射液(20μg.L-1)加LPD液灌注(进行肺灌洗及保存)。两组都在4℃保存2 h后再灌注1 h,测定经肺氧合后动脉血氧分压值(PaO2)和肺气道峰压值(PawP)。于再灌注结束后取右上肺组织,测其湿重(Wr)与干重(Wd)、计算湿/干重比(Wr/Wd);ELISA法检测超氧化物歧化酶(SOD)、丙二醛(MDA)含量;免疫组化法检测NF-κB的表达;并用光学显微镜和透射电子显微镜观察再灌注后肺组织结构的病理变化。结果:再灌注30 m in后,两组的PaO2逐渐降低,PawP逐渐升高,实验组PaO2降低程度及PawP的升高程度均低于对照组(P<0.01);实验组的SOD明显高于对照组,MDA及肺组织的NF-κB的表达低于对照组(P<0.01),肺组织的病理变化较对照组轻。结论:Lipo-PGE1注射液能减轻无心跳兔肺缺血/再灌注损伤,改善肺功能,具有肺保护功能。
