Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flowe...Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour.‘Shixia’,an economically important fruit crop in subtropical regions,we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci(PHAS loci),120 hairpin loci,producing abundant sRNAs,were identified by in-house protocols.Our results suggested that 56 miRNA-target pairs,2221-nt-PHAS loci,and 111 hairpin loci are involved in posttranscriptional gene silencing during longan reproductive development.Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled,including miR482-PHAS and miRN15.miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense,and miRN15 is a novel miRNA likely associated with a hairpin locus(HPL-056)to regulate strigolactone receptor gene DWARF14(D14)and the biogenesis of phasiRNAs from D14.These small RNAs are enriched in flower buds,suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.展开更多
Longan(Dimocarpus longan Lour.),as an edible fruit and traditional Chinese medicine,has been consumed for thousands of years.Longan pulp has abundant nutritional phytochemicals such as protein,carbohydrate,vitamin C,p...Longan(Dimocarpus longan Lour.),as an edible fruit and traditional Chinese medicine,has been consumed for thousands of years.Longan pulp has abundant nutritional phytochemicals such as protein,carbohydrate,vitamin C,polysaccharides,polyphenols,which shows multiple biological activities including antioxidant,immunomodulatory and antitumor effects.Longan pericarp also demonstrates biological activities because of its rich content of polysaccharides and polyphenols.This review summarizes the bioactive compounds and bioactivities of longan pulp and aims to provide comprehensive information for future development of longan as a functional health food.展开更多
CCCH(C3 H) Zinc finger(Znf) transcription factors(TFs), as a novel type of Znf gene, regulate the expression of genes by binding to their mRNAs and play important roles in plant growth and development and abiotic stre...CCCH(C3 H) Zinc finger(Znf) transcription factors(TFs), as a novel type of Znf gene, regulate the expression of genes by binding to their mRNAs and play important roles in plant growth and development and abiotic stress resistance.Longan(Dimocarpous longan) is a tropical/subtropical fruit tree of great economic importance in Southeast Asia.However, genomic information on C3 H and their functions in longan are still unknown. In this study, a comprehensive analysis of the longan C3 H(DlC3 H) gene family was carried out. A total of 49 DlC3 H genes in three clades were identified from the longan genome database. Characteristics of the genes were analyzed with respect to gene structure,motif composition, phylogenetic tree and potential functions. The analysis of alternative splicing(AS) events suggested that AS events in DlC3 H genes were related to the transformation from longan non-embryonic to embryonic cultures.Promoter analysis indicated that most of the DlC3 H genes included cis-acting elements associated with hormones and stresses responses. Quantitative real-time PCR(qRT-PCR) analysis indicated that 26 of the 49 DlC3 Hs, which possess methyl jasmonate(MeJA) and abscisic acid(ABA) responsive cis-acting elements, showed differential expression patterns under treatment with ABA, MeJA and their endogenous inhibitors, suggesting that DlC3 Hs might be involved in the ABA and MeJA signaling pathways. The expression profiles of 17 of the 49 DlC3 Hs in non-embryonic callus and three tissues of embryonic cultures showed that only five of the 17 DlC3 Hs had the same expression trends as the FPKM trends in transcriptome data;the expression levels of DlC3 H07/14/16/36/49 in embryogenic callus and DlC3 H04/38 in globular embryos were high, suggesting that they have different functions in embryonic development. Further, we verified that DlC3 H01/03/05/11/19/39 were regulated by sRNAs by a modified 5’ RLM-RACE method. This study provides the first systematic analysis of C3 H genes in longan, and found that C3 H genes may be involved in hormone and stress responses, and somatic embryogenesis. Our preliminary investigation may provide clues to further studies on the characteristics and functions of this family in longan.展开更多
[Objectives]This study aimed to optimize the extraction process of longan(Dimocarpus longan Lour.)leaves by multi-indicator comprehensive evaluation method.[Methods]The contents of gallic acid ethyl ester,astragalin,q...[Objectives]This study aimed to optimize the extraction process of longan(Dimocarpus longan Lour.)leaves by multi-indicator comprehensive evaluation method.[Methods]The contents of gallic acid ethyl ester,astragalin,quercetin,luteolin and kaempferol in longan leaves were determined simultaneously by HPLC.Using the multi-indicator comprehensive evaluation method,the methanol concentration,solid to liquid ratio and extraction time for extraction of longan leaves were optimized by orthogonal test.[Results]The optimal extraction process of longan leaves are as follows:methanol concentration of 100%,solid to liquid ratio of 1∶5,and extraction time of 50 min.[Conclusions]The optimized process is simple and feasible,and it can be used to determine the contents of different ingredients in longan leaves.展开更多
[Objective] The study aimed to provide supports for developing chilling and freezing injury monitoring and disaster damage assessment of longan(Dimocarpus Longan Lour.).[Method] Based on field observation data,the rel...[Objective] The study aimed to provide supports for developing chilling and freezing injury monitoring and disaster damage assessment of longan(Dimocarpus Longan Lour.).[Method] Based on field observation data,the relationships between longan canopy temperature and air temperature under different weather types(sunny,cloudy to sunny,cloudy,rainy,radiation chilling injury and advection chilling injury)in 2007-2008 winter were analyzed.[Result] Diurnal variations of longan canopy temperature under sunny and radiation chilling injury weather conditions were most dramatic,followed with those under cloudy to sunny condition,while variations under cloudy,rainy and advection chilling injury conditions were mild.Diurnal variations of orchard air temperature were also closely related to weather types.By using linear and curvilinear regression methods,the relationship models between longan canopy temperature and observation station air temperature were established.The models for cloudy,rainy and advection chilling injury had better effects than those for sunny,cloudy to sunny and radiation chilling injury;the models for night were better than those for daytime and the whole day.[Conclusion] To some extent,applying the relationship models between longan canopy temperature and observation station air temperature could make up the shortcoming of meteorological data which were higher than the real values.展开更多
[Objectives] This study was conducted to analyze the internal causes of flower reversal in Longan （ Dimocarpus longan Lour.） trees. [Methods] With flowering trees and flower reversal trees as experimental materials,...[Objectives] This study was conducted to analyze the internal causes of flower reversal in Longan （ Dimocarpus longan Lour.） trees. [Methods] With flowering trees and flower reversal trees as experimental materials, the variations in sugar and starch in mature leaves, tender leaves, mature branches, twigs and terminal buds after flower forcing were analyzed. [Results] During flowering process, sugar content showed the greatest difference between flowering and flower reversal trees, and the difference was the greatest in mature leaves. Trees with mature leaves having a sugar content above 44.71 mg/g were found to be more prone to flowering, while those with leaf sugar content lower than 27.80 mg/g were susceptible to flower reversal. In addition, longan trees with a higher sugar content in tender leaves were not prone to flower reversal. [Conclusions] In future, whether off-season flower forcing can be performed on longan trees could be judged through the detection of tree leaves, which is of great significance to prevention of flower reversal in off-season longan production.展开更多
【目的】SWEET(sugars will eventually be exported transporters)是一类参与植物生长发育多个过程的糖转运蛋白,分析DlSWEET1基因在龙眼不同组织和处理下的表达,探究其在果实糖积累中的功能。【方法】以龙眼松风本果实为材料,克隆DlSW...【目的】SWEET(sugars will eventually be exported transporters)是一类参与植物生长发育多个过程的糖转运蛋白,分析DlSWEET1基因在龙眼不同组织和处理下的表达,探究其在果实糖积累中的功能。【方法】以龙眼松风本果实为材料,克隆DlSWEET1基因,采用实时荧光定量PCR分析DlSWEET1在龙眼不同组织器官的表达以及在激素、冷、热、干旱胁迫下的表达模式。通过亚细胞定位、糖转运活性分析及草莓瞬时转化研究DlSWEET1基因的功能。【结果】DlSWEET1基因开放阅读框(ORF)全长为750 bp,编码249个氨基酸,包含一个PQ-loop保守结构域和蛋白典型保守结构域MtN3_slv。DlSWEET1在龙眼根、茎、叶、果肉等组织中均有不同程度的表达,在叶中的表达量较高,在果肉中的表达量次之,而在茎和根中表达量较低;不同浓度的蔗糖、葡萄糖和果糖处理龙眼叶片后,DlSWEET1在叶片中的表达量均有显著升高;低温、干旱及MeJA(茉莉酸甲酯)处理可显著提高DlSWEET1的表达。农杆菌侵染本氏烟草发现DlSWEET1蛋白定位在细胞膜和细胞核。糖转运活性分析证明DlSWEET1蛋白可以转运葡萄糖、果糖、蔗糖和甘露糖。草莓中瞬时转化DlSWEET1可以显著提升果实中的可溶性糖含量。【结论】瞬时过表达DlSWEET1导致转基因草莓果实的可溶性糖含量增加,为进一步解析DlSWEET1在龙眼果实糖积累中的作用提供理论依据。展开更多
Objective To investigate the chemical constituents of Dimocarpus longan seeds in Sapindaceae.Methods The chemical constituents were isolated from the ethanol extract of D.longan seeds by silica gel column chromatograp...Objective To investigate the chemical constituents of Dimocarpus longan seeds in Sapindaceae.Methods The chemical constituents were isolated from the ethanol extract of D.longan seeds by silica gel column chromatography.Their structures were identified on the basis of physical and chemical properties and spectral analysis.Results One compound was isolated and identified as 2-methyl-1,10-undecanediol,named longandiol(1).Conclusion Compound 1 is a new compound.展开更多
基金funded by the National Key Research and Developmental Program of China(Grant No.2018YFD1000104)supported by awards from the National Natural Science Foundation of China(Grant Nos.32002009 and 32072547)The Special Support Program of Guangdong Province(Grant No.2019TX05N193).
文摘Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour.‘Shixia’,an economically important fruit crop in subtropical regions,we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci(PHAS loci),120 hairpin loci,producing abundant sRNAs,were identified by in-house protocols.Our results suggested that 56 miRNA-target pairs,2221-nt-PHAS loci,and 111 hairpin loci are involved in posttranscriptional gene silencing during longan reproductive development.Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled,including miR482-PHAS and miRN15.miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense,and miRN15 is a novel miRNA likely associated with a hairpin locus(HPL-056)to regulate strigolactone receptor gene DWARF14(D14)and the biogenesis of phasiRNAs from D14.These small RNAs are enriched in flower buds,suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.
基金This project was supported by the National Natural Science Foundation of China(No.31570348).
文摘Longan(Dimocarpus longan Lour.),as an edible fruit and traditional Chinese medicine,has been consumed for thousands of years.Longan pulp has abundant nutritional phytochemicals such as protein,carbohydrate,vitamin C,polysaccharides,polyphenols,which shows multiple biological activities including antioxidant,immunomodulatory and antitumor effects.Longan pericarp also demonstrates biological activities because of its rich content of polysaccharides and polyphenols.This review summarizes the bioactive compounds and bioactivities of longan pulp and aims to provide comprehensive information for future development of longan as a functional health food.
基金funded by the National Natural Science Foundation of China(31672127 and 31572088)the Scientific Research Foundation of Horticulture College of Fujian Agriculture and Forestry University,China(2018S02)+1 种基金the Construction of Plateau Discipline of Fujian Province,China(102/71201801101)the Project of Fujian Academy of Agricultural Sciences,China(AB2017-4)。
文摘CCCH(C3 H) Zinc finger(Znf) transcription factors(TFs), as a novel type of Znf gene, regulate the expression of genes by binding to their mRNAs and play important roles in plant growth and development and abiotic stress resistance.Longan(Dimocarpous longan) is a tropical/subtropical fruit tree of great economic importance in Southeast Asia.However, genomic information on C3 H and their functions in longan are still unknown. In this study, a comprehensive analysis of the longan C3 H(DlC3 H) gene family was carried out. A total of 49 DlC3 H genes in three clades were identified from the longan genome database. Characteristics of the genes were analyzed with respect to gene structure,motif composition, phylogenetic tree and potential functions. The analysis of alternative splicing(AS) events suggested that AS events in DlC3 H genes were related to the transformation from longan non-embryonic to embryonic cultures.Promoter analysis indicated that most of the DlC3 H genes included cis-acting elements associated with hormones and stresses responses. Quantitative real-time PCR(qRT-PCR) analysis indicated that 26 of the 49 DlC3 Hs, which possess methyl jasmonate(MeJA) and abscisic acid(ABA) responsive cis-acting elements, showed differential expression patterns under treatment with ABA, MeJA and their endogenous inhibitors, suggesting that DlC3 Hs might be involved in the ABA and MeJA signaling pathways. The expression profiles of 17 of the 49 DlC3 Hs in non-embryonic callus and three tissues of embryonic cultures showed that only five of the 17 DlC3 Hs had the same expression trends as the FPKM trends in transcriptome data;the expression levels of DlC3 H07/14/16/36/49 in embryogenic callus and DlC3 H04/38 in globular embryos were high, suggesting that they have different functions in embryonic development. Further, we verified that DlC3 H01/03/05/11/19/39 were regulated by sRNAs by a modified 5’ RLM-RACE method. This study provides the first systematic analysis of C3 H genes in longan, and found that C3 H genes may be involved in hormone and stress responses, and somatic embryogenesis. Our preliminary investigation may provide clues to further studies on the characteristics and functions of this family in longan.
基金National Natural Science Foundation of China(81560691)Special Fund Project for Innovation Driven Development of Guangxi(Gui Ke AA17202046)+7 种基金Open Program for Construction of TCM Pharmacy Doctor Station in Guangxi University of Chinese Medicine(201410-06)The First-Class Subject of Traditional Chinese Medicine(Ethnic Pharmacy)in Guangxi(Gui Jiao Ke Yan[2018]No.12)Program of Collaborative Innovation Center of Zhuang and Yao Medicine(Gui Jiao Ke Yan[2013]No.20)Program of Key Laboratory of Zhuang and Yao Medicine[Gui Ke Ji Zi[2014]No.32]The Eighth Batch of Special Experts Project of Guangxi Zhuang Autonomous Region(Study on Quality Standard of Zhuang&Yao Medicine,[2019]No.13)High-level Talent Team Cultivation Program of"Qihuang Project"of Guangxi University of Chinese Medicine(2018002)High Level Innovation Team and Excellent Scholar Program of Guangxi Universities(Gui Jiao Shi Fan[2019]No.52)Guangxi One Thousand Young and Middle-aged College and University Backbone Teachers Cultivation Program(Gui Jiao Ren[2019]No.5).
文摘[Objectives]This study aimed to optimize the extraction process of longan(Dimocarpus longan Lour.)leaves by multi-indicator comprehensive evaluation method.[Methods]The contents of gallic acid ethyl ester,astragalin,quercetin,luteolin and kaempferol in longan leaves were determined simultaneously by HPLC.Using the multi-indicator comprehensive evaluation method,the methanol concentration,solid to liquid ratio and extraction time for extraction of longan leaves were optimized by orthogonal test.[Results]The optimal extraction process of longan leaves are as follows:methanol concentration of 100%,solid to liquid ratio of 1∶5,and extraction time of 50 min.[Conclusions]The optimized process is simple and feasible,and it can be used to determine the contents of different ingredients in longan leaves.
基金Supported by National Key Project of Scientific and Technical Support-ing Programs Funded by Ministry of Science & Technology of China during the11thFive-Year Plan Period"Study on Monitoring,Early Warning and Control Techniques of Major Agricultural Meteorological Disasters--Study on Monitoring and Early Warning Techniques of Chilling Injury in South China"(2006BAD04B03)Subject of National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Tech-nology of China"Study on Chilling and Freezing Injuries Assessment,Mo-nitoring and Warning Techniques of Main Subtropical Crops"(2008BADB8B01)~~
文摘[Objective] The study aimed to provide supports for developing chilling and freezing injury monitoring and disaster damage assessment of longan(Dimocarpus Longan Lour.).[Method] Based on field observation data,the relationships between longan canopy temperature and air temperature under different weather types(sunny,cloudy to sunny,cloudy,rainy,radiation chilling injury and advection chilling injury)in 2007-2008 winter were analyzed.[Result] Diurnal variations of longan canopy temperature under sunny and radiation chilling injury weather conditions were most dramatic,followed with those under cloudy to sunny condition,while variations under cloudy,rainy and advection chilling injury conditions were mild.Diurnal variations of orchard air temperature were also closely related to weather types.By using linear and curvilinear regression methods,the relationship models between longan canopy temperature and observation station air temperature were established.The models for cloudy,rainy and advection chilling injury had better effects than those for sunny,cloudy to sunny and radiation chilling injury;the models for night were better than those for daytime and the whole day.[Conclusion] To some extent,applying the relationship models between longan canopy temperature and observation station air temperature could make up the shortcoming of meteorological data which were higher than the real values.
基金Supported by State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(SKLCUSA-b201601)Non-profit Research Institution Reform Start-up Fund for Tropical Crop Germplasm Research Institute,Chinese Academy of Tropical Agricultural Sciences(pzsfyl-201804)China Agriculture Research System(CARS-33-27)
文摘[Objectives] This study was conducted to analyze the internal causes of flower reversal in Longan （ Dimocarpus longan Lour.） trees. [Methods] With flowering trees and flower reversal trees as experimental materials, the variations in sugar and starch in mature leaves, tender leaves, mature branches, twigs and terminal buds after flower forcing were analyzed. [Results] During flowering process, sugar content showed the greatest difference between flowering and flower reversal trees, and the difference was the greatest in mature leaves. Trees with mature leaves having a sugar content above 44.71 mg/g were found to be more prone to flowering, while those with leaf sugar content lower than 27.80 mg/g were susceptible to flower reversal. In addition, longan trees with a higher sugar content in tender leaves were not prone to flower reversal. [Conclusions] In future, whether off-season flower forcing can be performed on longan trees could be judged through the detection of tree leaves, which is of great significance to prevention of flower reversal in off-season longan production.
文摘【目的】SWEET(sugars will eventually be exported transporters)是一类参与植物生长发育多个过程的糖转运蛋白,分析DlSWEET1基因在龙眼不同组织和处理下的表达,探究其在果实糖积累中的功能。【方法】以龙眼松风本果实为材料,克隆DlSWEET1基因,采用实时荧光定量PCR分析DlSWEET1在龙眼不同组织器官的表达以及在激素、冷、热、干旱胁迫下的表达模式。通过亚细胞定位、糖转运活性分析及草莓瞬时转化研究DlSWEET1基因的功能。【结果】DlSWEET1基因开放阅读框(ORF)全长为750 bp,编码249个氨基酸,包含一个PQ-loop保守结构域和蛋白典型保守结构域MtN3_slv。DlSWEET1在龙眼根、茎、叶、果肉等组织中均有不同程度的表达,在叶中的表达量较高,在果肉中的表达量次之,而在茎和根中表达量较低;不同浓度的蔗糖、葡萄糖和果糖处理龙眼叶片后,DlSWEET1在叶片中的表达量均有显著升高;低温、干旱及MeJA(茉莉酸甲酯)处理可显著提高DlSWEET1的表达。农杆菌侵染本氏烟草发现DlSWEET1蛋白定位在细胞膜和细胞核。糖转运活性分析证明DlSWEET1蛋白可以转运葡萄糖、果糖、蔗糖和甘露糖。草莓中瞬时转化DlSWEET1可以显著提升果实中的可溶性糖含量。【结论】瞬时过表达DlSWEET1导致转基因草莓果实的可溶性糖含量增加,为进一步解析DlSWEET1在龙眼果实糖积累中的作用提供理论依据。
基金Science and Technology Planning Project of Guangdong Province,China(2010B020312006)Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences(KSCXZ-YW-R-218)
文摘Objective To investigate the chemical constituents of Dimocarpus longan seeds in Sapindaceae.Methods The chemical constituents were isolated from the ethanol extract of D.longan seeds by silica gel column chromatography.Their structures were identified on the basis of physical and chemical properties and spectral analysis.Results One compound was isolated and identified as 2-methyl-1,10-undecanediol,named longandiol(1).Conclusion Compound 1 is a new compound.
