With the increasing planting area of Lycium barbarum L.,influence of diseases and pests has grown more serious,and the researches on the diseases and pests of L. barbarum L. have become increasingly important. This st...With the increasing planting area of Lycium barbarum L.,influence of diseases and pests has grown more serious,and the researches on the diseases and pests of L. barbarum L. have become increasingly important. This study reviewed the found diseases and pests of L. barbarum L. and corresponding control measures,proposed the problems,suggestions and prospects,in order to provide references for the sustainable healthy development of L. barbarum L. industry in the Ningxia Hui Autonomous Region.展开更多
In order to utilize the wasted saline-sodic soils under shallow groundwater condition,a 3-year field study was carried in a field cropped with Lycium barbarum L.and irrigated by drip irrigation with saline groundwater...In order to utilize the wasted saline-sodic soils under shallow groundwater condition,a 3-year field study was carried in a field cropped with Lycium barbarum L.and irrigated by drip irrigation with saline groundwater under the water table depth of 30-40 cm in the northern Yinchuan Plain,China.Effects of cropping duration (one,two,and three years) on soil salinity,soil solution composition,and pH in three adjacent plots were investigated in 2008.Results showed that a high irrigation frequency maintained high soil water potential and subsequently facilitated infiltration and downward movement of water and salt in the crop root zone.Salt accumulated on the edges of the ridges,and soil saturated-paste electrical conductivity (ECe) was higher in the edge.Concentrations of Na+,Ca2+,Mg2+,Cl-,and SO42- in the soil increased with the soil depth as did the ECe,while HCO3- and pH had a relative uniform distribution in soil profile.As planting year increased,the ECe and soil salts in the field had a decreasing tendency,while in the root zone they decreased immediately after irrigation and then remained relatively stable in the following growing seasons.HCO3- and pH had little change with the planting year.Results suggested that the application of drip irrigation with saline water could ameliorate saline-sodic soil and provide a relatively feasible soil environment for the growth of salt-tolerant plant Lycium barbarum L.under the saline-sodic soils with shallow groundwater.展开更多
A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C...A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was展开更多
基金Sponsored by Innovation Team of Beifang University of Nationalities(TDXM-2014-006)Innovation Test of Beifang University of Nationalities(XJCX-2014-107)
文摘With the increasing planting area of Lycium barbarum L.,influence of diseases and pests has grown more serious,and the researches on the diseases and pests of L. barbarum L. have become increasingly important. This study reviewed the found diseases and pests of L. barbarum L. and corresponding control measures,proposed the problems,suggestions and prospects,in order to provide references for the sustainable healthy development of L. barbarum L. industry in the Ningxia Hui Autonomous Region.
基金Supported by the Chinese Academy of Sciences Action Plan for the Development of Western China (No. KZCX2-XB2-13)the Chinese Academy of Sciences Knowledge Innovation Program (No. KSCX2-YW-N-003)the 100 Talents Program of Chinese Academy of Sciences
文摘In order to utilize the wasted saline-sodic soils under shallow groundwater condition,a 3-year field study was carried in a field cropped with Lycium barbarum L.and irrigated by drip irrigation with saline groundwater under the water table depth of 30-40 cm in the northern Yinchuan Plain,China.Effects of cropping duration (one,two,and three years) on soil salinity,soil solution composition,and pH in three adjacent plots were investigated in 2008.Results showed that a high irrigation frequency maintained high soil water potential and subsequently facilitated infiltration and downward movement of water and salt in the crop root zone.Salt accumulated on the edges of the ridges,and soil saturated-paste electrical conductivity (ECe) was higher in the edge.Concentrations of Na+,Ca2+,Mg2+,Cl-,and SO42- in the soil increased with the soil depth as did the ECe,while HCO3- and pH had a relative uniform distribution in soil profile.As planting year increased,the ECe and soil salts in the field had a decreasing tendency,while in the root zone they decreased immediately after irrigation and then remained relatively stable in the following growing seasons.HCO3- and pH had little change with the planting year.Results suggested that the application of drip irrigation with saline water could ameliorate saline-sodic soil and provide a relatively feasible soil environment for the growth of salt-tolerant plant Lycium barbarum L.under the saline-sodic soils with shallow groundwater.
文摘A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was