The Mao Dun Literature Prize is one of the well-known literary awards in China,and in the process of selection over the years,the Mao Dun Literature Prize has developed its own aesthetic orientation.A comparison is ma...The Mao Dun Literature Prize is one of the well-known literary awards in China,and in the process of selection over the years,the Mao Dun Literature Prize has developed its own aesthetic orientation.A comparison is made between the early generation of Xu Mao and His Daughters and the recent award-winning work,A Tale of the Wind,to discover the aesthetic heritage in its flux,which is enlightening for the literary criticism and an orientation for the literary creators.展开更多
目的观察单胺氧化酶A(monoamine oxidase A,MAO-A)对与3T3-L1脂肪细胞共培养的原代新生大鼠心肌细胞(neonatal rat cardiomyocyte,NRCM)脂质沉积的影响及机制。方法选取40只出生1~3天的SD大鼠心肌细胞与3T3-L1脂肪细胞构建NRCM/3T3-L1...目的观察单胺氧化酶A(monoamine oxidase A,MAO-A)对与3T3-L1脂肪细胞共培养的原代新生大鼠心肌细胞(neonatal rat cardiomyocyte,NRCM)脂质沉积的影响及机制。方法选取40只出生1~3天的SD大鼠心肌细胞与3T3-L1脂肪细胞构建NRCM/3T3-L1共培养体系,并加入棕榈酸(palmitic acid,PA),模拟肥胖时心肌细胞所处的内环境。将细胞分为对照组、PA组、去甲肾上腺素(norepinephrine,NE)组、过氧化氢(hydrogen peroxide,H 2O 2)组、NE+CLG组、氯吉兰(clorgyline,CLG)组。采用α横纹肌肌动蛋白评估心肌细胞的纯度和面积,实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)测定心肌细胞内心房利钠肽(atrial natriuretic peptide,ANP)的mRNA水平,免疫荧光染色测定心肌细胞内活性氧簇水平(reactive oxygen species,ROS),油红O染色评估心肌细胞内脂质沉积,Western blot法测定心肌细胞内MAO-A、JNK的蛋白及磷酸化水平。结果与对照组比较,PA组心肌细胞面积(14.681±0.481μm^(2) vs 19.932±0.220μm^(2),P<0.01)增大,ANP的相对mRNA水平(1.006±0.017 vs 1.914±0.152,P<0.01)、MAO-A的相对蛋白水平(0.117±0.022 vs 0.430±0.092,P<0.01)、ROS水平(0.001±0.001 vs 0.004±0.002,P<0.01)、脂滴面积比(0.001±0.001 vs 0.054±0.002,P<0.01)及p-JNK的相对蛋白水平(0.032±0.007 vs 0.263±0.119,P<0.05)均增加。与PA组比较,NE明显增加心肌细胞内MAO-A的相对蛋白水平(0.430±0.092 vs 1.114±0.091,P<0.01)和ROS水平(0.004±0.002 vs 0.013±0.001,P<0.01)。另外,NE和H 2O 2均能增加心肌细胞脂滴面积比(0.054±0.002 vs 0.106±0.005,P<0.05;0.054±0.002 vs 0.092±0.002,P<0.01)和p-JNK的相对蛋白水平(0.263±0.119 vs 0.969±0.082,P<0.05;0.263±0.119 vs 0.640±0.101,P<0.05)。CLG可明显改善NE诱导的异常。结论MAO-A相对蛋白水平增加会加重与3T3-L1脂肪细胞共培养的心肌细胞内的氧化应激和脂质沉积,其机制可能与ROS/JNK信号级联反应激活有关。展开更多
This paper explores Mao Zedong’s early views on physical education and practice,focusing on the“Six-Section Exercise”he created.The exercise,which integrates elements from traditional health-preserving techniques,g...This paper explores Mao Zedong’s early views on physical education and practice,focusing on the“Six-Section Exercise”he created.The exercise,which integrates elements from traditional health-preserving techniques,gymnastics,boxing,and military training,was designed to promote balanced physical development,be practical and effective,and require less time per session.The paper argues that Mao Zedong’s views on physical education were grounded in the belief that it could effectively transform individuals and contribute to the creation of a strong sports nation.The paper also discusses the historical context and social trends behind the creation of the“Six-Section Exercise”,including the New Culture Movement and debates between Chinese and Western,static and dynamic,and new and old approaches to physical education.The paper concludes by suggesting that Mao Zedong’s early sports thinking has significant academic value and can provide guidance for the construction of a healthy China today.展开更多
文摘The Mao Dun Literature Prize is one of the well-known literary awards in China,and in the process of selection over the years,the Mao Dun Literature Prize has developed its own aesthetic orientation.A comparison is made between the early generation of Xu Mao and His Daughters and the recent award-winning work,A Tale of the Wind,to discover the aesthetic heritage in its flux,which is enlightening for the literary criticism and an orientation for the literary creators.
文摘目的观察单胺氧化酶A(monoamine oxidase A,MAO-A)对与3T3-L1脂肪细胞共培养的原代新生大鼠心肌细胞(neonatal rat cardiomyocyte,NRCM)脂质沉积的影响及机制。方法选取40只出生1~3天的SD大鼠心肌细胞与3T3-L1脂肪细胞构建NRCM/3T3-L1共培养体系,并加入棕榈酸(palmitic acid,PA),模拟肥胖时心肌细胞所处的内环境。将细胞分为对照组、PA组、去甲肾上腺素(norepinephrine,NE)组、过氧化氢(hydrogen peroxide,H 2O 2)组、NE+CLG组、氯吉兰(clorgyline,CLG)组。采用α横纹肌肌动蛋白评估心肌细胞的纯度和面积,实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)测定心肌细胞内心房利钠肽(atrial natriuretic peptide,ANP)的mRNA水平,免疫荧光染色测定心肌细胞内活性氧簇水平(reactive oxygen species,ROS),油红O染色评估心肌细胞内脂质沉积,Western blot法测定心肌细胞内MAO-A、JNK的蛋白及磷酸化水平。结果与对照组比较,PA组心肌细胞面积(14.681±0.481μm^(2) vs 19.932±0.220μm^(2),P<0.01)增大,ANP的相对mRNA水平(1.006±0.017 vs 1.914±0.152,P<0.01)、MAO-A的相对蛋白水平(0.117±0.022 vs 0.430±0.092,P<0.01)、ROS水平(0.001±0.001 vs 0.004±0.002,P<0.01)、脂滴面积比(0.001±0.001 vs 0.054±0.002,P<0.01)及p-JNK的相对蛋白水平(0.032±0.007 vs 0.263±0.119,P<0.05)均增加。与PA组比较,NE明显增加心肌细胞内MAO-A的相对蛋白水平(0.430±0.092 vs 1.114±0.091,P<0.01)和ROS水平(0.004±0.002 vs 0.013±0.001,P<0.01)。另外,NE和H 2O 2均能增加心肌细胞脂滴面积比(0.054±0.002 vs 0.106±0.005,P<0.05;0.054±0.002 vs 0.092±0.002,P<0.01)和p-JNK的相对蛋白水平(0.263±0.119 vs 0.969±0.082,P<0.05;0.263±0.119 vs 0.640±0.101,P<0.05)。CLG可明显改善NE诱导的异常。结论MAO-A相对蛋白水平增加会加重与3T3-L1脂肪细胞共培养的心肌细胞内的氧化应激和脂质沉积,其机制可能与ROS/JNK信号级联反应激活有关。
文摘This paper explores Mao Zedong’s early views on physical education and practice,focusing on the“Six-Section Exercise”he created.The exercise,which integrates elements from traditional health-preserving techniques,gymnastics,boxing,and military training,was designed to promote balanced physical development,be practical and effective,and require less time per session.The paper argues that Mao Zedong’s views on physical education were grounded in the belief that it could effectively transform individuals and contribute to the creation of a strong sports nation.The paper also discusses the historical context and social trends behind the creation of the“Six-Section Exercise”,including the New Culture Movement and debates between Chinese and Western,static and dynamic,and new and old approaches to physical education.The paper concludes by suggesting that Mao Zedong’s early sports thinking has significant academic value and can provide guidance for the construction of a healthy China today.
