To optimize the experimental conditions of MTT colorimetric assay for HSS bioactivity in vitro,we studied the optimal combination of the major conditions of the MTT assay by orthogonal test and other experiments,and c...To optimize the experimental conditions of MTT colorimetric assay for HSS bioactivity in vitro,we studied the optimal combination of the major conditions of the MTT assay by orthogonal test and other experiments,and compared HSS bioactivity in vitro measured by the improved MTT protocol and published MTT assay at serial protein doses.Results showed that the absorbance value(A value)of the MTT assay directly correlated with the number of human hepatoma cell lines SMMC7721.The result of orthogonal test was the number of 5×104 SMMC7721 cells/ml,culture period 6 h before adding HSS,concentration of HSS 100μg/ml,incubation time with HSS 36 h.Additionally,several experiments demonstrated the optimal combination of other conditions was 50μg MTT,incubation time for MTT 6 h,DMSO was used to dissolve the MTT formazan crystals and measured with ELISA scanner at 570 nm.The result of determining HSS bio-activity in vitro by optimized MTT protocol showed that sHSS bio-activity increased with the growth of protein dose,but decreased when it beyond a certain dose.The optimized MTT protocol was a sensitive,convenient and stable quantitative method to evaluate HSS bio-activity.展开更多
Biocompatible conversion of chitosan and chitosan/silica hybrid coating were prepared to enhance the biocompatibility and corrosion resistance of biodegradable AZ31 Mg alloy. The coatings were optimized and analysed w...Biocompatible conversion of chitosan and chitosan/silica hybrid coating were prepared to enhance the biocompatibility and corrosion resistance of biodegradable AZ31 Mg alloy. The coatings were optimized and analysed with potentiodynamic polarization, SEM, ATR-IR and XPS studies. Potentiodynamic polarization studies, revealed that the coatings exhibited high corrosion resistance. The surface morphology of the Ch-3/Si coating showed small globular rough structure. The presence of functional groups was confirmed by ATR-IR. For a better understanding of chitosan/silica hybrid coating, the chemical states were examined by XPS studies. The in-vitro bioactivity of the coated samples was evaluated in Earle’s solution, which formed a dense layer of coral-like structure and calcium-deficient apatite with less stoichiometric ratio than the hydroxyapatite. In-vitro cell culture studies exhibited a good cell proliferation rate and the fabricated Ch-3/Si coating was found to be non-hemolytic. The bacterial studies proved that Ch-3/Si coating possessed inherent antibacterial activity.展开更多
Objective: To assess the antiangiogcnic activity of fenugreek.Methods: Different fractions of fenugreek crude extracts were prepared and their antiangiogenic properties were assessed using the ex vivo rat aortic ring ...Objective: To assess the antiangiogcnic activity of fenugreek.Methods: Different fractions of fenugreek crude extracts were prepared and their antiangiogenic properties were assessed using the ex vivo rat aortic ring assay and in vivo chicken embryo chorioallantoic membrane(CAM) assay. They were investigated for their direct cytotoxic activity in the MCF7 cells using the MTT assay.Results: The ethanol extract showed 100% inhibition of blood vessel outgrowth from primary tissue explants in the rat aortic ring assay at a concentration of 100μg/mL while the other extracts did not show significant antiangiogenic activity. The ethanol extract was therefore investigated at varying concentrations and exhibited a significant dose dependent effect. The CAM assay coincided with the results of the aortic ring assay as ethanol extract showed a significant inhibition of formation of new blood vessels. The extracts only showed anti-proliferative activity at the highest concentration of 400μg/mL towards MCF7 breast cancer cell lines in the MTT assay.Conclusions: Findings of the both assays confirmed that the ethanol extract inhibited vascularization significantly. Further studies on the ethanol extract would be beneficial in isolating the active ingredient responsible for the inhibition.展开更多
Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Method...Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe^(2+) ion chelating activity, and in vitro antiproliferative effects(sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe^(2+) ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.展开更多
The effects of different doses of swainonine( SW),thermopsine( Ts) and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totally 24...The effects of different doses of swainonine( SW),thermopsine( Ts) and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totally 240 mice were randomly grouped,and orally administrated with different concentrations of SW,Ts and their mixture. Four mice were randomly selected from each group at the 7^(th),14^(th),21^(st) and 35^(th) days,and lymphocytes were separated from anti-coagulated blood collected from eyeball. After Con A stimulation,T lymphocyte proliferation was detected by MTT assay. The results showed that low dose of SW promoted proliferation of mouse T lymphocyte; medium and high dose of SW also promoted proliferation of mouse T lymphocyte within a short period,but would seriously suppress its proliferation after a long period. Low dose of Ts almost had no impact on proliferation of T lymphocyte,while high dose also suppressed its proliferation.A similar result with SW was observed on alkaloid mixture. This indicated that different doses of SW,Ts and their mixture affected proliferation of T lymphocyte in different degrees.展开更多
The genus Claviceps (Clavicipitaceae) is noted for producing ergot alkaloids that cause ergotism. Claviceps yanagawaensis, a parasite of Zoysia japonica (family: Poaceae), has been isolated in Japan. Bioactivity scree...The genus Claviceps (Clavicipitaceae) is noted for producing ergot alkaloids that cause ergotism. Claviceps yanagawaensis, a parasite of Zoysia japonica (family: Poaceae), has been isolated in Japan. Bioactivity screening showed that a methanol extract from a rice culture of C. yanagawaensis was cytotoxic to cancer cells. In our search for active substances, the new secalonic acid analogues (-)-5-epi-F-7 (1) and ergochrysin C (2) and a new benzoic acid analogue, dimethyl bigutol (3), were isolated along with the known compounds 3,4-dihydroxy-5-(3-methyl-2-buten-1-yl)benzoic acid (4) and methyl veratrate (5). The structures of 1 - 3 were elucidated by NMR, MS, and circular dichroism spectroscopy. MTT assays of 1 - 5 using cancer cell lines (HepG2, HL60, HT29, PANC-1, and T98G) showed that 1 - 4 exhibited cytotoxicity against cancer cells.展开更多
The article evaluated the degradation of the captopril in aqueous solution after ozonation and chlorination. The process was continuously monitored focusing on the identification, mass spectrometry and elucidation of ...The article evaluated the degradation of the captopril in aqueous solution after ozonation and chlorination. The process was continuously monitored focusing on the identification, mass spectrometry and elucidation of its by-products by applying direct infusion and high performance liquid chromatography, electrospray ionization high resolution mass spectrometry, in the negative ion mode. The cytotoxicity of its by-products solutions were evaluated with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. It was observed through that after 30 min of ozonation and chlorination, there was complete oxidation of captopril, i.e., 100% removal efficiency. At these conditions, the rate of mineralization, by total organic carbon, was only 7.63% for ozonation and 6.40% for chlorination, evidencing the formation of degradation by-products. Ten captopril by-products were identified and their respective chemical structures elucidations are proposed. The treated samples and their by-products were nontoxic to HepG2 cells by MTT assay.展开更多
The bioactive secondary metabolites from the endophytic fungus Fusarium napiforme was evaluated for the cytotoxic effect and antioxidant activity.The total antioxidant capacity(TAC)of the extract was determined by 2,2...The bioactive secondary metabolites from the endophytic fungus Fusarium napiforme was evaluated for the cytotoxic effect and antioxidant activity.The total antioxidant capacity(TAC)of the extract was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH),phosphomolybdate,and reducing power assay methods.The cytotoxicity of the extract was evaluated against lung adenocarcinoma(A549)cells and mouse embryo fibroblast(NIH3T3)cells by methyl thiazolyl tetrazolium(MTT)method.The major composition of the crude extract was identified by the Gas Chromatography-Mass Spectrometry(GC-MS)analysis.Estimation of the endophyte crude extract revealed a high amount of the total flavonoid content(TFC)and total phenolic content(TPC).The extract showed high cytotoxic activity against the A549 cell line with the mean cytotoxicity of 69.74±0.49%.The extract did not show any cytotoxic effect against the NIH3T3 cell line.The extract exhibited high antioxidant activity as a function of the concentrations.At a test concentration of 1 mg ml-1,the extract showed the highest inhibition against DPPH radical at 75.4607%±0.47688,ferric ion reducing power at 0.882±0.0120,and 255.434±21.404 AAE/g of the extract by phosphomolybdenum assay(PMA).There is a significant correlation between TPC and antioxidant activity at p<0.05.The correlation between reducing power and DPPH is significant at p<0.01.The major types of bioactive compounds identified by the GC-MS have shown the presence of nine major compounds.This result strongly exhibits that the endophyte F.napiforme can be a potential source for the formulation of natural anticancer drugs and protecting the body from oxidative damages.展开更多
Gold nanoparticles recently showed great interest for many uses including food, drug and medical applications. The algae </span><i style="font-family:"white-space:normal;">Undaria</i...Gold nanoparticles recently showed great interest for many uses including food, drug and medical applications. The algae </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> well known as wakame in South Asia are considered to be large edible brown algae. It provides nutritious source of dietary fiber, vitamin Bs and mineral. The present study aimed to investigate the use of </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> for green synthesis of metallic gold nanoparticles. The synthesized nanoparticles were characterized for physicochemical properties including size measurement and tested </span><i style="font-family:"white-space:normal;">in</i><span style="font-family:"white-space:normal;"> <i>vitro</i></span><span style="font-family:"white-space:normal;"> for their effect on viability of human non-small lung cancer H-460 cell line using the MTT assay. From the results, brown algae were able to chemically form nanoparticles with chloroauric acid solution possibly due to the sulphated polysaccharides found in algae. The particle sizes were found to be approximately 10 nm. The gold nanoparticles stabilized by the algae could decrease the cancer cell viability. However, the properties and biological activity of nanoparticles seemed to depend upon reaction time and temperature. Conclusively, gold nanoparticles synthesized and stabilized by the algae could decrease the cancer cell viability, thus indicating the potential of such nanoparticles for further study for anticancer activity.展开更多
Hand,foot,and mouth disease(HFMD)is a contagious disease mainly occurring in young children,and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand.Moreover,the World Health Or...Hand,foot,and mouth disease(HFMD)is a contagious disease mainly occurring in young children,and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand.Moreover,the World Health Organization(WHO)monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance System.HFMD is mainly caused by a group of enteroviruses(EVs)transmitted through direct contact(person to person)and indirect contact with contaminated objects(surface-to-hand).However,few studies have examined the surface stability of EVs.In this study,we investigated the stability of enterovirus A71(EV-A71)and coxsackievirus A16(CVA16)on three different dry surfaces(wood,plastic,and stainless steel)using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)staining of viable cells and real-time polymerase chain reaction(viral genome detection).The results revealed that virus infectivity dramatically decreased within a few hours on dry surfaces.However,viral RNA could be detected on dry surfaces for up to 28 days.Concerning heat inactivation,both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 min.Information on virus stability on different dry surfaces will provide useful information for HFMD transmission control.展开更多
In this study we examined the suitability of the 3H-imidazo[4,5-b]pyridine ring system in developing novel anticancer and anti-inflammatory agents incorporating a diaryl pharmacophore. Eight 2,3-diaryl-3H-imidazo[4,5-...In this study we examined the suitability of the 3H-imidazo[4,5-b]pyridine ring system in developing novel anticancer and anti-inflammatory agents incorporating a diaryl pharmacophore. Eight 2,3-diaryl-3H-imidazo[4,5-b]pyridine derivatives retrieved from our in-house database were evaluated for their cytotoxic activity against nine cancer cell lines. The results indicated that the compounds showed moderate cytotoxic activity against MCF-7, MDA-MB-468, K562 and SaOS2 cells, with K562 being the most sensitive among the four cancer cell lines. The eight 2,3-diaryl-3H-imidazo[4,5-b]pyridine derivatives were also evaluated for their COX-1 and COX-2 inhibitory activity in vitro. The results showed that compound 3f exhibited 2-fold selectivity with IC_(50) values of 9.2 and 21.8 mmol/L against COX-2 and COX-1, respectively. Molecular docking studies on the most active compound 3f revealed a binding mode similar to that of celecoxib in the active site of the COX-2 enzyme.展开更多
Novel organic compounds(1~8) were designed, synthesized and characterized by IR, ~1H NMR, H RMS, and single-crystal X-ray crystallography. The anticancer activities of these compounds were investigated against four hu...Novel organic compounds(1~8) were designed, synthesized and characterized by IR, ~1H NMR, H RMS, and single-crystal X-ray crystallography. The anticancer activities of these compounds were investigated against four human lung cancer cells(H20, H2227, H69 and H524) by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) assay. Furthermore, by taking compounds 1 and 5 as representatives, molecular docking studies supported the biological assay data, suggesting that compared with 1, compound 5 has stronger interaction with protein.展开更多
Two new isochroman derivatives(1 and 2)were isolated from the fermentation products of mangrove endophytic fungus Phomopsis sp.33#.Their structures were characterized by IR,~1 H NMR,H RMS,and single-crystal X-ray crys...Two new isochroman derivatives(1 and 2)were isolated from the fermentation products of mangrove endophytic fungus Phomopsis sp.33#.Their structures were characterized by IR,~1 H NMR,H RMS,and single-crystal X-ray crystallography.The anticancer activity of the two compounds was investigated against four human lung cancer cells(NCI-H292,NCI-H460,LTEP-A-2 and MSTO-211)by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay.Furthermore,molecular docking studies supported the biological assay data,showing that compared with 1,compound 2 has stronger interaction with protein.展开更多
文摘To optimize the experimental conditions of MTT colorimetric assay for HSS bioactivity in vitro,we studied the optimal combination of the major conditions of the MTT assay by orthogonal test and other experiments,and compared HSS bioactivity in vitro measured by the improved MTT protocol and published MTT assay at serial protein doses.Results showed that the absorbance value(A value)of the MTT assay directly correlated with the number of human hepatoma cell lines SMMC7721.The result of orthogonal test was the number of 5×104 SMMC7721 cells/ml,culture period 6 h before adding HSS,concentration of HSS 100μg/ml,incubation time with HSS 36 h.Additionally,several experiments demonstrated the optimal combination of other conditions was 50μg MTT,incubation time for MTT 6 h,DMSO was used to dissolve the MTT formazan crystals and measured with ELISA scanner at 570 nm.The result of determining HSS bio-activity in vitro by optimized MTT protocol showed that sHSS bio-activity increased with the growth of protein dose,but decreased when it beyond a certain dose.The optimized MTT protocol was a sensitive,convenient and stable quantitative method to evaluate HSS bio-activity.
文摘Biocompatible conversion of chitosan and chitosan/silica hybrid coating were prepared to enhance the biocompatibility and corrosion resistance of biodegradable AZ31 Mg alloy. The coatings were optimized and analysed with potentiodynamic polarization, SEM, ATR-IR and XPS studies. Potentiodynamic polarization studies, revealed that the coatings exhibited high corrosion resistance. The surface morphology of the Ch-3/Si coating showed small globular rough structure. The presence of functional groups was confirmed by ATR-IR. For a better understanding of chitosan/silica hybrid coating, the chemical states were examined by XPS studies. The in-vitro bioactivity of the coated samples was evaluated in Earle’s solution, which formed a dense layer of coral-like structure and calcium-deficient apatite with less stoichiometric ratio than the hydroxyapatite. In-vitro cell culture studies exhibited a good cell proliferation rate and the fabricated Ch-3/Si coating was found to be non-hemolytic. The bacterial studies proved that Ch-3/Si coating possessed inherent antibacterial activity.
基金financially supported by the Deanship of academic Research (#1616),the University of Jordan,Amman,Jordan
文摘Objective: To assess the antiangiogcnic activity of fenugreek.Methods: Different fractions of fenugreek crude extracts were prepared and their antiangiogenic properties were assessed using the ex vivo rat aortic ring assay and in vivo chicken embryo chorioallantoic membrane(CAM) assay. They were investigated for their direct cytotoxic activity in the MCF7 cells using the MTT assay.Results: The ethanol extract showed 100% inhibition of blood vessel outgrowth from primary tissue explants in the rat aortic ring assay at a concentration of 100μg/mL while the other extracts did not show significant antiangiogenic activity. The ethanol extract was therefore investigated at varying concentrations and exhibited a significant dose dependent effect. The CAM assay coincided with the results of the aortic ring assay as ethanol extract showed a significant inhibition of formation of new blood vessels. The extracts only showed anti-proliferative activity at the highest concentration of 400μg/mL towards MCF7 breast cancer cell lines in the MTT assay.Conclusions: Findings of the both assays confirmed that the ethanol extract inhibited vascularization significantly. Further studies on the ethanol extract would be beneficial in isolating the active ingredient responsible for the inhibition.
基金supported by grants from FAPES(Fundacao de Amparo a Pesquisa e Inovacao do Espirito Santo)-term of grant 225/2015
文摘Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe^(2+) ion chelating activity, and in vitro antiproliferative effects(sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe^(2+) ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.
基金Supported by Natural Science Foundation of Inner Mongolia(2012MS0404)
文摘The effects of different doses of swainonine( SW),thermopsine( Ts) and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totally 240 mice were randomly grouped,and orally administrated with different concentrations of SW,Ts and their mixture. Four mice were randomly selected from each group at the 7^(th),14^(th),21^(st) and 35^(th) days,and lymphocytes were separated from anti-coagulated blood collected from eyeball. After Con A stimulation,T lymphocyte proliferation was detected by MTT assay. The results showed that low dose of SW promoted proliferation of mouse T lymphocyte; medium and high dose of SW also promoted proliferation of mouse T lymphocyte within a short period,but would seriously suppress its proliferation after a long period. Low dose of Ts almost had no impact on proliferation of T lymphocyte,while high dose also suppressed its proliferation.A similar result with SW was observed on alkaloid mixture. This indicated that different doses of SW,Ts and their mixture affected proliferation of T lymphocyte in different degrees.
文摘The genus Claviceps (Clavicipitaceae) is noted for producing ergot alkaloids that cause ergotism. Claviceps yanagawaensis, a parasite of Zoysia japonica (family: Poaceae), has been isolated in Japan. Bioactivity screening showed that a methanol extract from a rice culture of C. yanagawaensis was cytotoxic to cancer cells. In our search for active substances, the new secalonic acid analogues (-)-5-epi-F-7 (1) and ergochrysin C (2) and a new benzoic acid analogue, dimethyl bigutol (3), were isolated along with the known compounds 3,4-dihydroxy-5-(3-methyl-2-buten-1-yl)benzoic acid (4) and methyl veratrate (5). The structures of 1 - 3 were elucidated by NMR, MS, and circular dichroism spectroscopy. MTT assays of 1 - 5 using cancer cell lines (HepG2, HL60, HT29, PANC-1, and T98G) showed that 1 - 4 exhibited cytotoxicity against cancer cells.
基金the Minas Gerais State Science Foundation(FAPEMIG)-process APQ-00918-13the Brazilian National Research Council(CNPq)-process 473893-2013-1 for their financial support and the granting of research fellowships.
文摘The article evaluated the degradation of the captopril in aqueous solution after ozonation and chlorination. The process was continuously monitored focusing on the identification, mass spectrometry and elucidation of its by-products by applying direct infusion and high performance liquid chromatography, electrospray ionization high resolution mass spectrometry, in the negative ion mode. The cytotoxicity of its by-products solutions were evaluated with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. It was observed through that after 30 min of ozonation and chlorination, there was complete oxidation of captopril, i.e., 100% removal efficiency. At these conditions, the rate of mineralization, by total organic carbon, was only 7.63% for ozonation and 6.40% for chlorination, evidencing the formation of degradation by-products. Ten captopril by-products were identified and their respective chemical structures elucidations are proposed. The treated samples and their by-products were nontoxic to HepG2 cells by MTT assay.
文摘The bioactive secondary metabolites from the endophytic fungus Fusarium napiforme was evaluated for the cytotoxic effect and antioxidant activity.The total antioxidant capacity(TAC)of the extract was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH),phosphomolybdate,and reducing power assay methods.The cytotoxicity of the extract was evaluated against lung adenocarcinoma(A549)cells and mouse embryo fibroblast(NIH3T3)cells by methyl thiazolyl tetrazolium(MTT)method.The major composition of the crude extract was identified by the Gas Chromatography-Mass Spectrometry(GC-MS)analysis.Estimation of the endophyte crude extract revealed a high amount of the total flavonoid content(TFC)and total phenolic content(TPC).The extract showed high cytotoxic activity against the A549 cell line with the mean cytotoxicity of 69.74±0.49%.The extract did not show any cytotoxic effect against the NIH3T3 cell line.The extract exhibited high antioxidant activity as a function of the concentrations.At a test concentration of 1 mg ml-1,the extract showed the highest inhibition against DPPH radical at 75.4607%±0.47688,ferric ion reducing power at 0.882±0.0120,and 255.434±21.404 AAE/g of the extract by phosphomolybdenum assay(PMA).There is a significant correlation between TPC and antioxidant activity at p<0.05.The correlation between reducing power and DPPH is significant at p<0.01.The major types of bioactive compounds identified by the GC-MS have shown the presence of nine major compounds.This result strongly exhibits that the endophyte F.napiforme can be a potential source for the formulation of natural anticancer drugs and protecting the body from oxidative damages.
文摘Gold nanoparticles recently showed great interest for many uses including food, drug and medical applications. The algae </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> well known as wakame in South Asia are considered to be large edible brown algae. It provides nutritious source of dietary fiber, vitamin Bs and mineral. The present study aimed to investigate the use of </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> for green synthesis of metallic gold nanoparticles. The synthesized nanoparticles were characterized for physicochemical properties including size measurement and tested </span><i style="font-family:"white-space:normal;">in</i><span style="font-family:"white-space:normal;"> <i>vitro</i></span><span style="font-family:"white-space:normal;"> for their effect on viability of human non-small lung cancer H-460 cell line using the MTT assay. From the results, brown algae were able to chemically form nanoparticles with chloroauric acid solution possibly due to the sulphated polysaccharides found in algae. The particle sizes were found to be approximately 10 nm. The gold nanoparticles stabilized by the algae could decrease the cancer cell viability. However, the properties and biological activity of nanoparticles seemed to depend upon reaction time and temperature. Conclusively, gold nanoparticles synthesized and stabilized by the algae could decrease the cancer cell viability, thus indicating the potential of such nanoparticles for further study for anticancer activity.
文摘Hand,foot,and mouth disease(HFMD)is a contagious disease mainly occurring in young children,and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand.Moreover,the World Health Organization(WHO)monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance System.HFMD is mainly caused by a group of enteroviruses(EVs)transmitted through direct contact(person to person)and indirect contact with contaminated objects(surface-to-hand).However,few studies have examined the surface stability of EVs.In this study,we investigated the stability of enterovirus A71(EV-A71)and coxsackievirus A16(CVA16)on three different dry surfaces(wood,plastic,and stainless steel)using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)staining of viable cells and real-time polymerase chain reaction(viral genome detection).The results revealed that virus infectivity dramatically decreased within a few hours on dry surfaces.However,viral RNA could be detected on dry surfaces for up to 28 days.Concerning heat inactivation,both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 min.Information on virus stability on different dry surfaces will provide useful information for HFMD transmission control.
文摘In this study we examined the suitability of the 3H-imidazo[4,5-b]pyridine ring system in developing novel anticancer and anti-inflammatory agents incorporating a diaryl pharmacophore. Eight 2,3-diaryl-3H-imidazo[4,5-b]pyridine derivatives retrieved from our in-house database were evaluated for their cytotoxic activity against nine cancer cell lines. The results indicated that the compounds showed moderate cytotoxic activity against MCF-7, MDA-MB-468, K562 and SaOS2 cells, with K562 being the most sensitive among the four cancer cell lines. The eight 2,3-diaryl-3H-imidazo[4,5-b]pyridine derivatives were also evaluated for their COX-1 and COX-2 inhibitory activity in vitro. The results showed that compound 3f exhibited 2-fold selectivity with IC_(50) values of 9.2 and 21.8 mmol/L against COX-2 and COX-1, respectively. Molecular docking studies on the most active compound 3f revealed a binding mode similar to that of celecoxib in the active site of the COX-2 enzyme.
基金supported by the Project of Inner Mongolia University for the Nationalities (No. NMDYB15064)Science Research Project of Inner Mongolia Education Department (No. NJZY20114)。
文摘Novel organic compounds(1~8) were designed, synthesized and characterized by IR, ~1H NMR, H RMS, and single-crystal X-ray crystallography. The anticancer activities of these compounds were investigated against four human lung cancer cells(H20, H2227, H69 and H524) by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) assay. Furthermore, by taking compounds 1 and 5 as representatives, molecular docking studies supported the biological assay data, suggesting that compared with 1, compound 5 has stronger interaction with protein.
基金supported by the Project of Inner Mongolia University for the Nationalities(No.NMDYB15064)Science Research Project of Inner Mongolia Education Department(No.NJZY20114)。
文摘Two new isochroman derivatives(1 and 2)were isolated from the fermentation products of mangrove endophytic fungus Phomopsis sp.33#.Their structures were characterized by IR,~1 H NMR,H RMS,and single-crystal X-ray crystallography.The anticancer activity of the two compounds was investigated against four human lung cancer cells(NCI-H292,NCI-H460,LTEP-A-2 and MSTO-211)by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay.Furthermore,molecular docking studies supported the biological assay data,showing that compared with 1,compound 2 has stronger interaction with protein.
