The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla L.cv.Longsang 1)mulberry leaves in Heilongjiang Province(China)and assess its obe...The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla L.cv.Longsang 1)mulberry leaves in Heilongjiang Province(China)and assess its obesity-preventing/relieving effects.A total of 174 compounds including quercetin,chlorogenic acid,1-deoxyecomycin(1-DNJ)related to antihyperlipidemia effects were identified from the MLIT powder.MLIT treatment reversed the Lee's index,fat coefficient,and serum biochemical parameters in both the obesity relieving and obesity preventing mice fed with high-fat diet.In the obesity relieving experiment,the relative abundance of Desulfovibrio in mouse feces decreased after both 0.5%and 1%MLIT treatments.In obesity preventing experiments,mouse with different amount of MLIT treatments showed increased relative abundance of Akkermansia,Bifidobacterium and Lactobacillus,while Deferribacteres,Desulfobacterota decreased.The beneficial bacteria in the intestinal tract of mice treated with MLIT increased.This study proved that MLIT had antihyperlipidemia potential via modulating intestinal microbiota in mice.展开更多
Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which coul...Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota.Hence,MLO 2-1 was selected for the further analysis.The structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1.The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota.Meanwhile,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and butyrate.The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated.Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM mice.Animal experiments indicated that MLO 2-1 and L.murinus exhibited hypoglycemic activities.These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L.murinus.展开更多
To provide a scientific basis for controlling mulberry bacterial blight in Bazhong,Sichuan,China(BSC),this study aimed to isolate and purify pathogenic bacteria from diseased branches of mulberry trees in the region a...To provide a scientific basis for controlling mulberry bacterial blight in Bazhong,Sichuan,China(BSC),this study aimed to isolate and purify pathogenic bacteria from diseased branches of mulberry trees in the region and to clarify their taxonomic status using morphological observation,physiological and biochemical detection,molecular-level identification,and the construction of a phylogenetic tree.A total of 218 bacterial strains were isolated from samples of diseased mulberry branches.Of these,7 strains were identified as pathogenic bacteria based on pathogenicity tests conducted in accordance with Koch’s postulates.Preliminary findings from the analysis of the 16S rRNA sequence indicated that the 7 pathogenic bacteria are members of Klebsiella spp.Morphological observation revealed that the pathogenic bacteria were oval-shaped and had capsules but no spores.They could secrete pectinase,cellulase,and protease and were able to utilize D-glucose,D-mannose,D-maltose,and D-Cellobiose.The 7 strains of pathogenic bacteria exhibited the highest homology with Klebsiella oxytoca.This study identifies Klebsiella oxytoca as the causative agent of mulberry bacterial blight in BSC,laying the foundation for the prevention and control of this pathogen and further investigation into its pathogenic mechanism.展开更多
Abiotic stress,including flooding,seriously affects the normal growth and development of plants.Mulberry(Morus alba),a species known for its flood resistance,is cultivated worldwide for economic purposes.The transcrip...Abiotic stress,including flooding,seriously affects the normal growth and development of plants.Mulberry(Morus alba),a species known for its flood resistance,is cultivated worldwide for economic purposes.The transcriptomic analysis has identified numerous differentially expressed genes(DEGs)involved in submergence tolerance in mulberry plants.However,a comprehensive analyses of metabolite types and changes under flooding stress in mulberry remain unreported.A non-targeted metabolomic analysis utilizing liquid chromatographytandem mass spectrometry(LC-MS/MS)was conducted to further investigate the effects of flooding stress on mulberry.A total of 1,169 metabolites were identified,with 331 differentially accumulated metabolites(DAMs)exhibiting up-regulation in response to flooding stress and 314 displaying down-regulation.Pathway enrichment analysis identified significant modifications in many metabolic pathways due to flooding stress,including amino acid biosynthesis and metabolism and flavonoid biosynthesis.DAMs and DEGs are significantly enriched in the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways for amino acid,phenylpropanoid and flavonoid synthesis.Furthermore,metabolites such as methyl jasmonate,sucrose,and D-mannose 6-phosphate accumulated in mulberry leaves post-flooding stress.Therefore,genes and metabolites associated with these KEGG pathways are likely to exert a significant influence on mulberry flood tolerance.This study makes a substantial contribution to the comprehension of the underlying mechanisms implicated in the adaptation of mulberry plants to submergence.展开更多
Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear...Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects.展开更多
[Objectives]This study to conducted to clarify various nutritional components and the composition and contents of aromatic substances in mulberry wine prepared by fermentation,and promote the quality improvement and p...[Objectives]This study to conducted to clarify various nutritional components and the composition and contents of aromatic substances in mulberry wine prepared by fermentation,and promote the quality improvement and product development of mulberry wine.[Methods]Mulberry wine was prepared with mulberry Shengguo 1 as the material using Saccharomyces cerevisiae,and its contents of total sugars,total phenols,total acids,resveratrol,protein,dry extract,ethanol content,Fe,methanol,sulfur dioxide,volatile acids,total flavonoids and proanthocyanidins were determined.Aromatic substances were extracted by n-hexane,and their components and relative contents were determined by gas chromatography-mass spectrometry(GC-MS).[Results]The physical and chemical indexes of the mulberry wine prepared by fermentation were in accordance with the national standards,and it was rich in resveratrol(10.5±0.6 mg/L),protein(381±11 mg/L),total flavonoids(406±18 mg/L)and proanthocyanidins(855±22 mg/L),which were 8.95,19.49,3.76 and 1.63 times higher than those of grape wine,respectively.The aromatic substances were mainly composed of ethyl formate(9.9%),ethyl acetate(7.6%),acetal(6.2%),propanol(5.7%),isobutanol(5.6%)and isoamyl acetate(5.3%).[Conclusions]The results of this study demonstrated that mulberry wine was rich in active substances,which suggested that mulberry wine has great potential in the fruit wine market.展开更多
[Objectives]This study was conducted to clarify the physiological mechanism of growth of hybrid mulberry after autumn cutting in herbaceous cultivation.[Methods]The net photosynthetic rate(Pn),stomatal conductance(Gs)...[Objectives]This study was conducted to clarify the physiological mechanism of growth of hybrid mulberry after autumn cutting in herbaceous cultivation.[Methods]The net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci)and transpiration rate(Tr)of leaves were measured under different treatment conditions using a Li-6400XT portable photosynthetic measurement system.[Results]After harvest of mulberry shoots in autumn,leaf Pn decreased with the extension of branch and leaf growth time,while Gs,Ci and Tr showed a trend of first increasing and then decreasing.The Pn was affected by factors such as leaf positions,mulberry varieties,cutting,and fertilization,which was manifested by the 6 th to 10 th mature leaves>the 2 nd to 4 th tender leaves,‘Nongsang 14’>hybrid mulberry,intermediate cut>uncut,and normal fertilization>no fertilization,all showing significant differences(P<0.05).Combined with the results of Gs,Ci and Tr measurements,it was found that the changes in leaf Pn were mainly related to non-stomatal factors.Timely cutting and harvesting during summer and autumn could significantly improve the photosynthetic rate of mulberry leaf,which was beneficial for extending the late autumn growth period of hybrid mulberry under herbaceous cultivation.[Conclusions]This study provides a theoretical reference for mulberry shoot harvesting techniques in summer and autumn.展开更多
Mulberry is a fundamental component of the global sericulture industry,and its positive impact on our health and the environment cannot be overstated.However,the mulberry reference genomes reported previously remained...Mulberry is a fundamental component of the global sericulture industry,and its positive impact on our health and the environment cannot be overstated.However,the mulberry reference genomes reported previously remained unassembled or unplaced sequences.Here,we report the assembly and analysis of the telomere-to-telomere gap-free reference genome of the mulberry species,Morus notabilis,which has emerged as an important reference in mulberry gene function research and genetic improvement.The mulberry gap-free reference genome produced here provides an unprecedented opportunity for us to study the structure and function of centromeres.Our results revealed that all mulberry centromeric regions share conserved centromeric satellite repeats with different copies.Strikingly,we found that M.notabilis is a species with polycentric chromosomes and the only reported polycentric chromosome species up to now.We propose a compelling model that explains the formation mechanism of new centromeres and addresses the unsolved scientific question of the chromosome fusion-fission cycle in mulberry species.Our study sheds light on the functional genomics,chromosome evolution,and genetic improvement of mulberry species.展开更多
Paper mulberry is a high-quality woody feed resource plant with high crude protein content.It is widely distributed in China and has excellent characteristics of salt and alkali tolerance.Paper mulberry has ecological...Paper mulberry is a high-quality woody feed resource plant with high crude protein content.It is widely distributed in China and has excellent characteristics of salt and alkali tolerance.Paper mulberry has ecological and economic importance.Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth.In the saline-alkali environments,transcriptome expression is altered leading to phenotypic defects in most plants.However,the regulatory mechanism related to paper mulberry’s salt-stress(SS)response is not clearly understood.In the present study de novo transcriptomic assembly was performed,and gene expression levels were measured between different SS and natural conditions(25℃)as a control for paper mulberry plants.According to the results of our study,under NaCl stress conditions,the differential gene expression was observed in the leaves of paper mulberry compared with the control.A total of 2126 differentially expressed unigenes were observed.Among these unigenes the expression of 812 DEGs was up-regulated and the expression of 1,314 DEGs was down-regulated.Additionally,The GO and KEGG analyses regarding differentially expressed unigenes(DEUs)revealed that the observed critical transcriptomic alterations under salt stress(SS)conditions were associated with primary and secondary metabolism,photosynthesis,and plant hormone signaling pathways.Further investigations such as gene function studies regarding the unigenes depicting altered expression under salt stress conditions in paper mulberry will help understand the mechanism of salt tolerance,and this information can be utilized in paper mulberry breeding and improvement programs.展开更多
[Objectives]In order to study the flower characteristics of mulberry and evaluate the resources of fruit mulberry.[Methods]Firstly,the resources of fruit mulberry were collected,and four varieties of fruit mulberry(‘...[Objectives]In order to study the flower characteristics of mulberry and evaluate the resources of fruit mulberry.[Methods]Firstly,the resources of fruit mulberry were collected,and four varieties of fruit mulberry(‘Dashi’mulberry,‘Changguo’milk mulberry,‘agate’mulberry and‘Xiangjin’milk mulberry)were collected.Then,the flower characteristics,fruit quality,and adaptability of fruit mulberry were studied.[Results]Mulberry is a dioecious plant,and the inflorescences are lurocatkin.The male flowers will fall off automatically,while the female flowers will develop into fruit.The four varieties of fruit mulberry have good adaptability in Shanghai,and their fruits have their own characteristics.At the same time,in order to use the resources of fruit mulberry,mulberry fruit picking activities were carried out for many times.[Conclusions]This study provides a reference for the cultivation,collection,utilization and evaluation of mulberry.展开更多
Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in...Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in clinical practice reflects the principle of“using medicine as a soldier”.In the treatment of diabetes mellitus and its complications,Professor Lv made good use of mulberry leaves,mulberry branches,mulberry white bark,mulberry and mulberry parasitism,and gave full play to the modern pharmacological effects of mulberry drugs such as hypoglycemic,hypotensive and lipidemic in the combined use,and achieved satisfactory results.展开更多
The aim of this study was to investigate changes of polyphenols content and the antioxidant activity of mulberry fruit powder after heating at high temperature.The mulberry fruit powder was heated by baking method at ...The aim of this study was to investigate changes of polyphenols content and the antioxidant activity of mulberry fruit powder after heating at high temperature.The mulberry fruit powder was heated by baking method at different temperature and time.The DPPH scavenging ability,the OH^(•-) scavenging ability and the O_(2)^(•-) scavenging ability were used to evaluate the changes in the antioxidant activity of the mulberry fruit powder after the heat treatment.The results showed that 74.87%of the polyphenols in the mulberry powder were lost after heating at 150℃for 5 min,and almost all the polyphenols were lost with the increase of the heating time and temperature.The antioxidant experiment showed that the DPPH scavenging rate,OH^(•-) scavenging rate and O_(2)^(•-) scavenging rate of mulberry fruit powder were 68.90%,39.15%and 14.09%,respectively after treatment at 150℃ for 5 min.Mulberry fruit powder still has some potential to be added to baked goods as a functional ingredient.展开更多
Abstract In the research, five test groups were set, including urea at O, 120, 240, 360 and 480 kg/hm^2, respectively, and analysis was conducted on mulberry leaf yield and some characters of the quality in order to e...Abstract In the research, five test groups were set, including urea at O, 120, 240, 360 and 480 kg/hm^2, respectively, and analysis was conducted on mulberry leaf yield and some characters of the quality in order to establish utility functions of ap-plied N in mulberry gardens with different production purposes and to provide schemes of rational N fertilization. The results indicated that yield of mulberry leaf and content and yield of active nutritional substances in the leaves would be reduced either by insufficient or excessive N quantity. In addition, 240 kg/hm^2 of applied N significantly improved active nutritional substances in leaf and 360 kg/hm^2 of applied N significantly improved yields of mulberry leaf and active nutritional sub-stances. Therefore, utility functions of applied N quantity with leaf yield, DNJ content and yield were established accordingly. The optimal N quantity in mulberry gardens for sericulture is recommended 360 kg/hm^2 and the optimal N quantities in mulberry gardens for increase of yield and content of DNJ are 299.56 and 294.72 kg/hm^2, respectively.展开更多
[Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin（DNJ）content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal val...[Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin（DNJ）content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal value.[Method] Effects of nitrogen amount（NO-3-N and NH＋4-N）and nitrogen form（NO-3-N/NH＋4-N）on DNJ content in mulberry leaf were studied by changing nitrogen amount and nitrogen form in water culture solution.[Result] DNJ contents in mulberry leaf both increased at first and then decreased with the increase of nitrogen amount,furthermore compared with NH＋4-N,NO-3-N was more beneficial to the accumulation of DNJ;DNJ content in mulberry leaf obviously increased at first and then decreased with the decrease of NO-3-N/NH＋4-N,among them when its rate was 25/75,DNJ content reached the highest.[Conclusion] Proper nitrogen amount and nitrogen form could effectively improve the DNJ content in mulberry leaf.展开更多
[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA ...[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA sequence of CYP305 B1 V1 Gene published in GenBank for wild mulberry silkworm, one pair of primers was designed, and the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm treated by NaF, rutin, cypermethrin and ecdysone was also analyzed by the semi - quantitative RT - PCR. Furthermore, homology comparison and phylogenetic analysis for amino acid sequences of this gene were studied. [ Result] Rutin, cypermethrin and NaF had effects on the expression of P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm, while ecdysone had no significant effect. Homology comparison for amino acids indicated that the amino acid sequence of this gene was the most similar to that of CYP305 B1 gene in Bombyx mori with 100% amino acid identity, and highly similar to those of Tribolium casmneum CYP305A1, Apis mellifera CYP305A1, Drosophi- la melanogaster CYP305A1, Anopheles gambiae CYP305A2and Culex pipiens quinquefasciatus CYP2LI. [ Conclusion] CYP305 B1 V1 Gene of wild mulberry silkworm is likely to mainly take part in the metabolism of exogenous compounds, which is of great significance for revealing the function of cytochrome P450 and the metabolic mechanism of different drugs.展开更多
Objective The purpose of this experiment was to investigate the effects of exogenous hormone factors on subculture multiplication and root induction of tis-suecultured seedlings in mulberry. [Method] The aseptic seedl...Objective The purpose of this experiment was to investigate the effects of exogenous hormone factors on subculture multiplication and root induction of tis-suecultured seedlings in mulberry. [Method] The aseptic seedlings of Guisangyou 12 were used as the materials and the comparative experiment on subculture multi-plication and root induction used different kinds,concentrations and combinations of exogenous hormones. [Result] The hormone combination of 0.1 mg/L IBA＋1.5 mg/L 6-BA＋0.03 mg/L TDZ showed the best effects to bud multiplication, and the multiple of bud multiplication was 5.28. The effects of NAA was better than IBA and IAA on root induction. The hormone combination of 2.0 mg/L NAA＋1.0 mg/L PP333 was most suitable to the root induction, in which the rooting rate arrived to 100%, the root number was 7.01 and the root length was 1.38 cm on average. [Conclusion] The results wil provide some technical reference for large-scale propagation of mul-berry seedlings in vitro.展开更多
[Objective] The aim was to research the effects of N, P and K fertilizers on photosynthetic characteristics of mulberry. [Method] In the test, Chuan 826, a va- riety of mulberry, was taken as an example to explore the...[Objective] The aim was to research the effects of N, P and K fertilizers on photosynthetic characteristics of mulberry. [Method] In the test, Chuan 826, a va- riety of mulberry, was taken as an example to explore the relationships of chloro- phyll content, leaf area index, net photosynthetic rate, stomatal conductance of leaf, CO2 concentration of cells in leaves and transpiration rate with N, P and K fertilizers at different quantities. [Result] N, P and K fertilizers at different quantities have ef- fects on photosynthetic indices. In detail, N, P and K fertilizer at appropriate quanti- ties enhanced LAI, Chla＋b, Pn, Cond and Tr, Ci of mulberry leaves in control group without fertilizers was significantly higher than that in treatment groups. N, P and K at proper quantities （N at 600 kg/hm2, P at 210 kg/hm2 and K at 300 kg/hm2） would keep functional leaves well and higher photosynthetic period for a long term. [Conclusion] The research explored adjustment of photosynthetic characteristics and differ- ent treatments on photosynthesis of mulberry, providing reference for construction of excellent and highly-qualified mulberry garden.展开更多
In this paper, the effect of different fertilizer treatments on the main indexes of growth and development and dry matter accumulation of perennial mulberry, which is cut and pruned in summer, were studied with ‘3414...In this paper, the effect of different fertilizer treatments on the main indexes of growth and development and dry matter accumulation of perennial mulberry, which is cut and pruned in summer, were studied with ‘3414’ field experiment design. The results showed that N, P, and K at proper amounts could promote mulberry growth and development, improve its dry matter content, increase its dry matter accumulation. The effects of N, P, K on leaf yield per plant were respectively expressed as follows： N2 N3 N1 N0 , P2 P1 P3 P0 , K2 K1 K3 K0 . It can be concluded that the recommended fertilizer amounts of dry matter accumulations in mulberry leaf were 694.36 kg/hm2 of N, 198.15 kg/hm2 of P, and 274.26 kg/hm2 of K and the dry matter achieved the maximum at 8 045.04 kg/hm2 . The recommended fertilizer amounts of accumulated dry matter in branch were 1 000.05 kg/hm2 of N, 242.04 kg/hm2 of P, and 218.01 kg/hm 2 of K, and the dry matter achieved the maximum at 5 969.05 kg/hm2 . The recommended fertilizer amount in young shoots were 883.76 kg/hm2 of N, 204.48 kg/hm2 of P and 426.59 kg/hm2 of K and dry matter achieved the maximum at 1 410.24 kg/hm2 . This paper could provide reference for the construction of highly-qualified mulberry field in Sichuan hilly area.展开更多
This paper study the effect of nitrogen （N, X1）, phosphorus （P, X2） and potassium （K, X3） in different amounts on crude protein, soluble sugar, total flavonoid and 1-deoxynojirimycin contents in mulberry leaves,...This paper study the effect of nitrogen （N, X1）, phosphorus （P, X2） and potassium （K, X3） in different amounts on crude protein, soluble sugar, total flavonoid and 1-deoxynojirimycin contents in mulberry leaves, with mulberry trees in spring and autumn as the material and as per ＂3414＂ experimental design. The results showed that the qualities and active substance content of mulberry leaves changed from increasing to decreasing with its development; crude protein and solu-ble sugar achieved the peak on August 20; total flavonoid and 1-deoxynojirimycin was the highest on May 15. Fertilizations with N, P and K fertilizers at different amounts had significant effects on quality of mulberry leaf and content of active substances. Specifically, as fertilizer amount increased, the content of active sub- stances grew dramatically and achieved the highest at level 2 （X^2X=X~_）. Based on fertilizer effect functions of objective yield, the recommended amounts of N, P and K fertilizers based on crude protein, soluble sugar, flavonoid content and DNJ in test sites were 718.46, 220.11 and 305.23 kg/hm2, when the highest of crude protein in mulberry leaf was 1 813.83 kg/hm2. When N, P and K fertilizers were recommended at 666.54, 204.41 and 243.18 kg/hm2, soluble sugar in mulberry leaf achieved the peak at 1 042.65 kg/hm2. When N, P and K fertilizers were at 675.96, 326.49 and 462.90 kg/hm2, flavonoid content achieved the maximum at 147.90 kg/hm2. When N, P and K fertilizers were at 720.9, 225.11 and 323.63 kg/hm2, DNJ content was the highest at 13.55 kg/hm2.展开更多
[Objective] This study was conducted to investigate effects of application amounts of N, P and K and their ratio on tea quality components in mulberry leaves. [Method] Effects of sole application and combined applicat...[Objective] This study was conducted to investigate effects of application amounts of N, P and K and their ratio on tea quality components in mulberry leaves. [Method] Effects of sole application and combined application of N, P and K on the yield and tea quality components of spring mulberry leaves were studied by 3-factor 3-level experiment, based on previous experiments. [Result] Combined ap- plication of 3 of N, P and K was better than sole application and combined appli- cation of 2 from them. Insufficient or excessive fertilization both significantly reduced yield of mulberry leaves and contents of tea quality components, such as 1-de- oxynojimycin （DNJ）, flavonoids, tea polyphenols and amino acids. With the yield of spring mulberry leaves as a target, it is advised to apply N, P and K at rates of 240, 150 and 60 kg/hm^2 , respectively. [Conclusion] This study provides reference and theoretical basis for the establishment of raw material base of high-quality mul- berry tea.展开更多
基金supported by the Natural Science Foundation of Heilongjiang Province (LH2021C075)Key Laboratory of Functional Inorganic Material Chemistry (Heilongjiang University),Ministry of Education。
文摘The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla L.cv.Longsang 1)mulberry leaves in Heilongjiang Province(China)and assess its obesity-preventing/relieving effects.A total of 174 compounds including quercetin,chlorogenic acid,1-deoxyecomycin(1-DNJ)related to antihyperlipidemia effects were identified from the MLIT powder.MLIT treatment reversed the Lee's index,fat coefficient,and serum biochemical parameters in both the obesity relieving and obesity preventing mice fed with high-fat diet.In the obesity relieving experiment,the relative abundance of Desulfovibrio in mouse feces decreased after both 0.5%and 1%MLIT treatments.In obesity preventing experiments,mouse with different amount of MLIT treatments showed increased relative abundance of Akkermansia,Bifidobacterium and Lactobacillus,while Deferribacteres,Desulfobacterota decreased.The beneficial bacteria in the intestinal tract of mice treated with MLIT increased.This study proved that MLIT had antihyperlipidemia potential via modulating intestinal microbiota in mice.
基金the Key Research and Development Program of Yunnan Province(No.202202AE090023)Key Research and Development Program of Guangdong Province(No.2022B0202040002+8 种基金2022B0202050001)the Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculturethe Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture Project(No.DT20220026)Talent Introduction Program of Guangdong Academy of Agricultural Sciences(No.R2022YJ-YB3004)the Basic and Applied Basic Research Project of Guangdong Province(No.2022A15151102272023A1515012386)the Science and Technology Planning Project of Guangzhou(No.2023A04J0828)the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams(No.202109TD)the Special Fund Project for Teachers’Scientific and Technological Achievements Transformation in Shunde Innovation Park,National University Science Park,South China University of Technology(No.KJYS2021KZ05)for their financial support。
文摘Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota.Hence,MLO 2-1 was selected for the further analysis.The structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1.The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota.Meanwhile,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and butyrate.The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated.Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM mice.Animal experiments indicated that MLO 2-1 and L.murinus exhibited hypoglycemic activities.These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L.murinus.
基金supported by Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(2021C02072-6)the Natural Science Foundation of Anhui Provincial Education Department(KJ2019A0574).
文摘To provide a scientific basis for controlling mulberry bacterial blight in Bazhong,Sichuan,China(BSC),this study aimed to isolate and purify pathogenic bacteria from diseased branches of mulberry trees in the region and to clarify their taxonomic status using morphological observation,physiological and biochemical detection,molecular-level identification,and the construction of a phylogenetic tree.A total of 218 bacterial strains were isolated from samples of diseased mulberry branches.Of these,7 strains were identified as pathogenic bacteria based on pathogenicity tests conducted in accordance with Koch’s postulates.Preliminary findings from the analysis of the 16S rRNA sequence indicated that the 7 pathogenic bacteria are members of Klebsiella spp.Morphological observation revealed that the pathogenic bacteria were oval-shaped and had capsules but no spores.They could secrete pectinase,cellulase,and protease and were able to utilize D-glucose,D-mannose,D-maltose,and D-Cellobiose.The 7 strains of pathogenic bacteria exhibited the highest homology with Klebsiella oxytoca.This study identifies Klebsiella oxytoca as the causative agent of mulberry bacterial blight in BSC,laying the foundation for the prevention and control of this pathogen and further investigation into its pathogenic mechanism.
基金The funding for this research was provided by the General Program of Chongqing Natural Science Foundation(No.cstc2020jcyj-msxmX0073)Scientific and Technological Research Program of Chongqing Municipal Education Commission(Nos.KJQN202001209,KJZD-K202301206)Chongqing Graduate Research Innovation Project(CYS22698).
文摘Abiotic stress,including flooding,seriously affects the normal growth and development of plants.Mulberry(Morus alba),a species known for its flood resistance,is cultivated worldwide for economic purposes.The transcriptomic analysis has identified numerous differentially expressed genes(DEGs)involved in submergence tolerance in mulberry plants.However,a comprehensive analyses of metabolite types and changes under flooding stress in mulberry remain unreported.A non-targeted metabolomic analysis utilizing liquid chromatographytandem mass spectrometry(LC-MS/MS)was conducted to further investigate the effects of flooding stress on mulberry.A total of 1,169 metabolites were identified,with 331 differentially accumulated metabolites(DAMs)exhibiting up-regulation in response to flooding stress and 314 displaying down-regulation.Pathway enrichment analysis identified significant modifications in many metabolic pathways due to flooding stress,including amino acid biosynthesis and metabolism and flavonoid biosynthesis.DAMs and DEGs are significantly enriched in the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways for amino acid,phenylpropanoid and flavonoid synthesis.Furthermore,metabolites such as methyl jasmonate,sucrose,and D-mannose 6-phosphate accumulated in mulberry leaves post-flooding stress.Therefore,genes and metabolites associated with these KEGG pathways are likely to exert a significant influence on mulberry flood tolerance.This study makes a substantial contribution to the comprehension of the underlying mechanisms implicated in the adaptation of mulberry plants to submergence.
基金the grant from National Key Research and Development Program of China(Grant No.2021YFE0110000)the grant from Tianjin Municipal Science and Technology Foundation(Grant No.22JCYBJC00160).
文摘Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects.
基金Supported by Scientific Research Program from Hubei Provincial Department of Education(B2021234)Open fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization(202303302).
文摘[Objectives]This study to conducted to clarify various nutritional components and the composition and contents of aromatic substances in mulberry wine prepared by fermentation,and promote the quality improvement and product development of mulberry wine.[Methods]Mulberry wine was prepared with mulberry Shengguo 1 as the material using Saccharomyces cerevisiae,and its contents of total sugars,total phenols,total acids,resveratrol,protein,dry extract,ethanol content,Fe,methanol,sulfur dioxide,volatile acids,total flavonoids and proanthocyanidins were determined.Aromatic substances were extracted by n-hexane,and their components and relative contents were determined by gas chromatography-mass spectrometry(GC-MS).[Results]The physical and chemical indexes of the mulberry wine prepared by fermentation were in accordance with the national standards,and it was rich in resveratrol(10.5±0.6 mg/L),protein(381±11 mg/L),total flavonoids(406±18 mg/L)and proanthocyanidins(855±22 mg/L),which were 8.95,19.49,3.76 and 1.63 times higher than those of grape wine,respectively.The aromatic substances were mainly composed of ethyl formate(9.9%),ethyl acetate(7.6%),acetal(6.2%),propanol(5.7%),isobutanol(5.6%)and isoamyl acetate(5.3%).[Conclusions]The results of this study demonstrated that mulberry wine was rich in active substances,which suggested that mulberry wine has great potential in the fruit wine market.
基金Supported by Suzhou Agricultural Science and Technology Planning Project (SNG2018093).
文摘[Objectives]This study was conducted to clarify the physiological mechanism of growth of hybrid mulberry after autumn cutting in herbaceous cultivation.[Methods]The net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci)and transpiration rate(Tr)of leaves were measured under different treatment conditions using a Li-6400XT portable photosynthetic measurement system.[Results]After harvest of mulberry shoots in autumn,leaf Pn decreased with the extension of branch and leaf growth time,while Gs,Ci and Tr showed a trend of first increasing and then decreasing.The Pn was affected by factors such as leaf positions,mulberry varieties,cutting,and fertilization,which was manifested by the 6 th to 10 th mature leaves>the 2 nd to 4 th tender leaves,‘Nongsang 14’>hybrid mulberry,intermediate cut>uncut,and normal fertilization>no fertilization,all showing significant differences(P<0.05).Combined with the results of Gs,Ci and Tr measurements,it was found that the changes in leaf Pn were mainly related to non-stomatal factors.Timely cutting and harvesting during summer and autumn could significantly improve the photosynthetic rate of mulberry leaf,which was beneficial for extending the late autumn growth period of hybrid mulberry under herbaceous cultivation.[Conclusions]This study provides a theoretical reference for mulberry shoot harvesting techniques in summer and autumn.
基金This project was supported by the National Natural Science Foundation of China(32101544)the Chongqing Research Program of Basic Research and Frontier Technology(cstc2021yszxjcyj0004).
文摘Mulberry is a fundamental component of the global sericulture industry,and its positive impact on our health and the environment cannot be overstated.However,the mulberry reference genomes reported previously remained unassembled or unplaced sequences.Here,we report the assembly and analysis of the telomere-to-telomere gap-free reference genome of the mulberry species,Morus notabilis,which has emerged as an important reference in mulberry gene function research and genetic improvement.The mulberry gap-free reference genome produced here provides an unprecedented opportunity for us to study the structure and function of centromeres.Our results revealed that all mulberry centromeric regions share conserved centromeric satellite repeats with different copies.Strikingly,we found that M.notabilis is a species with polycentric chromosomes and the only reported polycentric chromosome species up to now.We propose a compelling model that explains the formation mechanism of new centromeres and addresses the unsolved scientific question of the chromosome fusion-fission cycle in mulberry species.Our study sheds light on the functional genomics,chromosome evolution,and genetic improvement of mulberry species.
基金supported by the National Natural Science Foundation of China(31600552).
文摘Paper mulberry is a high-quality woody feed resource plant with high crude protein content.It is widely distributed in China and has excellent characteristics of salt and alkali tolerance.Paper mulberry has ecological and economic importance.Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth.In the saline-alkali environments,transcriptome expression is altered leading to phenotypic defects in most plants.However,the regulatory mechanism related to paper mulberry’s salt-stress(SS)response is not clearly understood.In the present study de novo transcriptomic assembly was performed,and gene expression levels were measured between different SS and natural conditions(25℃)as a control for paper mulberry plants.According to the results of our study,under NaCl stress conditions,the differential gene expression was observed in the leaves of paper mulberry compared with the control.A total of 2126 differentially expressed unigenes were observed.Among these unigenes the expression of 812 DEGs was up-regulated and the expression of 1,314 DEGs was down-regulated.Additionally,The GO and KEGG analyses regarding differentially expressed unigenes(DEUs)revealed that the observed critical transcriptomic alterations under salt stress(SS)conditions were associated with primary and secondary metabolism,photosynthesis,and plant hormone signaling pathways.Further investigations such as gene function studies regarding the unigenes depicting altered expression under salt stress conditions in paper mulberry will help understand the mechanism of salt tolerance,and this information can be utilized in paper mulberry breeding and improvement programs.
基金the Science and Technology Project for Agriculture Development of Shanghai Agricultural Commission[Hunongketuizi(2019)No.1-8].
文摘[Objectives]In order to study the flower characteristics of mulberry and evaluate the resources of fruit mulberry.[Methods]Firstly,the resources of fruit mulberry were collected,and four varieties of fruit mulberry(‘Dashi’mulberry,‘Changguo’milk mulberry,‘agate’mulberry and‘Xiangjin’milk mulberry)were collected.Then,the flower characteristics,fruit quality,and adaptability of fruit mulberry were studied.[Results]Mulberry is a dioecious plant,and the inflorescences are lurocatkin.The male flowers will fall off automatically,while the female flowers will develop into fruit.The four varieties of fruit mulberry have good adaptability in Shanghai,and their fruits have their own characteristics.At the same time,in order to use the resources of fruit mulberry,mulberry fruit picking activities were carried out for many times.[Conclusions]This study provides a reference for the cultivation,collection,utilization and evaluation of mulberry.
基金The Sixth Batch of National Academic Experience Inheritance Project for Senior Traditional Chinese Medicine Experts of State Administration of Traditional Chinese Medicine(No.JX332)National Traditional Chinese Medicine Experts Inheritance Studio Construction Project of State Administration of Traditional Chinese Medicine(No.JB063)。
文摘Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in clinical practice reflects the principle of“using medicine as a soldier”.In the treatment of diabetes mellitus and its complications,Professor Lv made good use of mulberry leaves,mulberry branches,mulberry white bark,mulberry and mulberry parasitism,and gave full play to the modern pharmacological effects of mulberry drugs such as hypoglycemic,hypotensive and lipidemic in the combined use,and achieved satisfactory results.
基金This project is funded by the Livelihood Plan Project of Department of Science and Technology of Liaoning Province(2021JH2/10300069,2019-ZD-0845)Department of Education of Liaoning Province(LJKZ0918)National College Students’Innovation and Entrepreneurship Training Program(202210163013).
文摘The aim of this study was to investigate changes of polyphenols content and the antioxidant activity of mulberry fruit powder after heating at high temperature.The mulberry fruit powder was heated by baking method at different temperature and time.The DPPH scavenging ability,the OH^(•-) scavenging ability and the O_(2)^(•-) scavenging ability were used to evaluate the changes in the antioxidant activity of the mulberry fruit powder after the heat treatment.The results showed that 74.87%of the polyphenols in the mulberry powder were lost after heating at 150℃for 5 min,and almost all the polyphenols were lost with the increase of the heating time and temperature.The antioxidant experiment showed that the DPPH scavenging rate,OH^(•-) scavenging rate and O_(2)^(•-) scavenging rate of mulberry fruit powder were 68.90%,39.15%and 14.09%,respectively after treatment at 150℃ for 5 min.Mulberry fruit powder still has some potential to be added to baked goods as a functional ingredient.
基金Supported by Special Fund of Modern Agricultural Industry Technology System(No.CARS-22)Special Fund of Major Industry Technology of Sichuan Economic and Trade Commission(No.2008NC00365)+1 种基金Excellent Paper Fund of Sichuan Academy of Agricultural Sciences(No.2011JYGC-LWJJ-07)Special Fund of Sichuan Financial Genetic Engineering(No.2011JYGC-QNJJ-021)~~
文摘Abstract In the research, five test groups were set, including urea at O, 120, 240, 360 and 480 kg/hm^2, respectively, and analysis was conducted on mulberry leaf yield and some characters of the quality in order to establish utility functions of ap-plied N in mulberry gardens with different production purposes and to provide schemes of rational N fertilization. The results indicated that yield of mulberry leaf and content and yield of active nutritional substances in the leaves would be reduced either by insufficient or excessive N quantity. In addition, 240 kg/hm^2 of applied N significantly improved active nutritional substances in leaf and 360 kg/hm^2 of applied N significantly improved yields of mulberry leaf and active nutritional sub-stances. Therefore, utility functions of applied N quantity with leaf yield, DNJ content and yield were established accordingly. The optimal N quantity in mulberry gardens for sericulture is recommended 360 kg/hm^2 and the optimal N quantities in mulberry gardens for increase of yield and content of DNJ are 299.56 and 294.72 kg/hm^2, respectively.
基金Supported by Key Technology Innovation Project of Sichuan Province(2008NC00365)~~
文摘[Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin（DNJ）content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal value.[Method] Effects of nitrogen amount（NO-3-N and NH＋4-N）and nitrogen form（NO-3-N/NH＋4-N）on DNJ content in mulberry leaf were studied by changing nitrogen amount and nitrogen form in water culture solution.[Result] DNJ contents in mulberry leaf both increased at first and then decreased with the increase of nitrogen amount,furthermore compared with NH＋4-N,NO-3-N was more beneficial to the accumulation of DNJ;DNJ content in mulberry leaf obviously increased at first and then decreased with the decrease of NO-3-N/NH＋4-N,among them when its rate was 25/75,DNJ content reached the highest.[Conclusion] Proper nitrogen amount and nitrogen form could effectively improve the DNJ content in mulberry leaf.
文摘[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA sequence of CYP305 B1 V1 Gene published in GenBank for wild mulberry silkworm, one pair of primers was designed, and the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm treated by NaF, rutin, cypermethrin and ecdysone was also analyzed by the semi - quantitative RT - PCR. Furthermore, homology comparison and phylogenetic analysis for amino acid sequences of this gene were studied. [ Result] Rutin, cypermethrin and NaF had effects on the expression of P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm, while ecdysone had no significant effect. Homology comparison for amino acids indicated that the amino acid sequence of this gene was the most similar to that of CYP305 B1 gene in Bombyx mori with 100% amino acid identity, and highly similar to those of Tribolium casmneum CYP305A1, Apis mellifera CYP305A1, Drosophi- la melanogaster CYP305A1, Anopheles gambiae CYP305A2and Culex pipiens quinquefasciatus CYP2LI. [ Conclusion] CYP305 B1 V1 Gene of wild mulberry silkworm is likely to mainly take part in the metabolism of exogenous compounds, which is of great significance for revealing the function of cytochrome P450 and the metabolic mechanism of different drugs.
基金Supported by Post-graduate Innovation Program of Guangxi(YCSZ2013007)~~
文摘Objective The purpose of this experiment was to investigate the effects of exogenous hormone factors on subculture multiplication and root induction of tis-suecultured seedlings in mulberry. [Method] The aseptic seedlings of Guisangyou 12 were used as the materials and the comparative experiment on subculture multi-plication and root induction used different kinds,concentrations and combinations of exogenous hormones. [Result] The hormone combination of 0.1 mg/L IBA＋1.5 mg/L 6-BA＋0.03 mg/L TDZ showed the best effects to bud multiplication, and the multiple of bud multiplication was 5.28. The effects of NAA was better than IBA and IAA on root induction. The hormone combination of 2.0 mg/L NAA＋1.0 mg/L PP333 was most suitable to the root induction, in which the rooting rate arrived to 100%, the root number was 7.01 and the root length was 1.38 cm on average. [Conclusion] The results wil provide some technical reference for large-scale propagation of mul-berry seedlings in vitro.
基金Supported by Research Fund for Excellent Thesis of Sichuan Academy of Agricultural Sciences(2011LWJJ-008)Younth Funds of Genetic Engineering of Sichuan Finance (2011QNJJ-021)Special Funds of Construction of Modern Agricultural Industry Technology System(CARS-22)~~
文摘[Objective] The aim was to research the effects of N, P and K fertilizers on photosynthetic characteristics of mulberry. [Method] In the test, Chuan 826, a va- riety of mulberry, was taken as an example to explore the relationships of chloro- phyll content, leaf area index, net photosynthetic rate, stomatal conductance of leaf, CO2 concentration of cells in leaves and transpiration rate with N, P and K fertilizers at different quantities. [Result] N, P and K fertilizers at different quantities have ef- fects on photosynthetic indices. In detail, N, P and K fertilizer at appropriate quanti- ties enhanced LAI, Chla＋b, Pn, Cond and Tr, Ci of mulberry leaves in control group without fertilizers was significantly higher than that in treatment groups. N, P and K at proper quantities （N at 600 kg/hm2, P at 210 kg/hm2 and K at 300 kg/hm2） would keep functional leaves well and higher photosynthetic period for a long term. [Conclusion] The research explored adjustment of photosynthetic characteristics and differ- ent treatments on photosynthesis of mulberry, providing reference for construction of excellent and highly-qualified mulberry garden.
基金Supported by Science&Technology Innovation of Sichuan Characteristic AgricultureProvincial Breeding Key Project of the 12th Five Year Plan (No.2011yzgg-13-02-01)+2 种基金Sichuan Academy of Agricultural Sciences (2011LWJJ-008)Seed Industry Innovation and Transformation Project (2011JYGC10-027-02)Modern Agricultural Technology System Project (No.CARS-22)~~
文摘In this paper, the effect of different fertilizer treatments on the main indexes of growth and development and dry matter accumulation of perennial mulberry, which is cut and pruned in summer, were studied with ‘3414’ field experiment design. The results showed that N, P, and K at proper amounts could promote mulberry growth and development, improve its dry matter content, increase its dry matter accumulation. The effects of N, P, K on leaf yield per plant were respectively expressed as follows： N2 N3 N1 N0 , P2 P1 P3 P0 , K2 K1 K3 K0 . It can be concluded that the recommended fertilizer amounts of dry matter accumulations in mulberry leaf were 694.36 kg/hm2 of N, 198.15 kg/hm2 of P, and 274.26 kg/hm2 of K and the dry matter achieved the maximum at 8 045.04 kg/hm2 . The recommended fertilizer amounts of accumulated dry matter in branch were 1 000.05 kg/hm2 of N, 242.04 kg/hm2 of P, and 218.01 kg/hm 2 of K, and the dry matter achieved the maximum at 5 969.05 kg/hm2 . The recommended fertilizer amount in young shoots were 883.76 kg/hm2 of N, 204.48 kg/hm2 of P and 426.59 kg/hm2 of K and dry matter achieved the maximum at 1 410.24 kg/hm2 . This paper could provide reference for the construction of highly-qualified mulberry field in Sichuan hilly area.
基金Supported by Crop Breeding Key Program of the 12th Five-year Plan(No.2011yzgg-1302-01)Sichuan Academy of Agricultural Sciences(2011LWJJ-008)+1 种基金Sichuan Financial Genetic Engineering(2011JYGC10-027-02)Special Fund of Modern Agricultural Industry Technology System Construction(No.CARS-22)~~
文摘This paper study the effect of nitrogen （N, X1）, phosphorus （P, X2） and potassium （K, X3） in different amounts on crude protein, soluble sugar, total flavonoid and 1-deoxynojirimycin contents in mulberry leaves, with mulberry trees in spring and autumn as the material and as per ＂3414＂ experimental design. The results showed that the qualities and active substance content of mulberry leaves changed from increasing to decreasing with its development; crude protein and solu-ble sugar achieved the peak on August 20; total flavonoid and 1-deoxynojirimycin was the highest on May 15. Fertilizations with N, P and K fertilizers at different amounts had significant effects on quality of mulberry leaf and content of active substances. Specifically, as fertilizer amount increased, the content of active sub- stances grew dramatically and achieved the highest at level 2 （X^2X=X~_）. Based on fertilizer effect functions of objective yield, the recommended amounts of N, P and K fertilizers based on crude protein, soluble sugar, flavonoid content and DNJ in test sites were 718.46, 220.11 and 305.23 kg/hm2, when the highest of crude protein in mulberry leaf was 1 813.83 kg/hm2. When N, P and K fertilizers were recommended at 666.54, 204.41 and 243.18 kg/hm2, soluble sugar in mulberry leaf achieved the peak at 1 042.65 kg/hm2. When N, P and K fertilizers were at 675.96, 326.49 and 462.90 kg/hm2, flavonoid content achieved the maximum at 147.90 kg/hm2. When N, P and K fertilizers were at 720.9, 225.11 and 323.63 kg/hm2, DNJ content was the highest at 13.55 kg/hm2.
基金Supported by Financial Innovation Ability Improving Engineering Project in Sichuan Province(2016TSCY-006)Technological Innovation Fund for Small and Medium Technology-based Enterprises of Nanchong City(15C0009)Nanchong Science and Technology Planning Project-Special Fund for Innovation-driven Development(16CXQD0021)~~
文摘[Objective] This study was conducted to investigate effects of application amounts of N, P and K and their ratio on tea quality components in mulberry leaves. [Method] Effects of sole application and combined application of N, P and K on the yield and tea quality components of spring mulberry leaves were studied by 3-factor 3-level experiment, based on previous experiments. [Result] Combined ap- plication of 3 of N, P and K was better than sole application and combined appli- cation of 2 from them. Insufficient or excessive fertilization both significantly reduced yield of mulberry leaves and contents of tea quality components, such as 1-de- oxynojimycin （DNJ）, flavonoids, tea polyphenols and amino acids. With the yield of spring mulberry leaves as a target, it is advised to apply N, P and K at rates of 240, 150 and 60 kg/hm^2 , respectively. [Conclusion] This study provides reference and theoretical basis for the establishment of raw material base of high-quality mul- berry tea.
