Objective:As a traditional herbal formula,Mahuang Xixin Fuzi decoction(MXFD)has been used to treat allergic rhinitis(AR).It regulates the transcription factor GATA3 in T cells,blocks Th2 skewing and rebalances the Th1...Objective:As a traditional herbal formula,Mahuang Xixin Fuzi decoction(MXFD)has been used to treat allergic rhinitis(AR).It regulates the transcription factor GATA3 in T cells,blocks Th2 skewing and rebalances the Th1/Th2 immunity.Type 2 innate lymphoid cells(ILC2s)are closely associated with GATA3.However,it remains unknown whether ILC2s could be one mechanism through which MXFD acts.We sought to elucidate the efficacy and mechanism of action of this decoction in AR treatment.Methods:Forty C57BL/6J female mice were equally divided into control,model,loratadine-and MXFDtreated groups in random.AR was induced by ovalbumin(OVA),and then treatment with loratadine or MXFD was administered.AR scores were evaluated and compared before and after treatment.Pathological changes in the nasal mucosa and lung were observed using hematoxylin-eosin staining of tissue samples.Activation of ILC2 in nasal mucosa was assessed by immunofluorescence and quantitative polymerase chain reaction analysis.ILC2 cell count in lungs was measured by flow cytometry and levels of interleukin-(IL)4,IL-5 and IL-13 in serum were detected by ELISA.Results:The decoction alleviated the symptoms of AR in mice,improved vascular congestion and expansion,glandular hyperplasia and inflammatory cell infiltration in mouse nasal mucosa and slowly improved the interstitial pneumonia and lesions.Meanwhile,MXFD reduced the number and percentage of ILC2s in the nasal mucosa and lungs,downregulated the expression of GATA3 mRNA and RORa mRNA,and reduced the related inflammatory cytokine levels,including IL-4,IL-5 and IL-13.Conclusion:These data suggest that inhibition of ILC2s by MXFD may be an important means by which to treat AR.展开更多
目的探索加味麻黄附子细辛汤治疗腰椎间盘突出症疗效优势人群和和使用禁忌,凸显六经辨证特色和优势。方法选取2019年3月~2020年3月就诊于上海市浦东新区中医医院骨伤门诊患者,采用队列研究分析将运用加味麻黄附子细辛汤结合中医综合治...目的探索加味麻黄附子细辛汤治疗腰椎间盘突出症疗效优势人群和和使用禁忌,凸显六经辨证特色和优势。方法选取2019年3月~2020年3月就诊于上海市浦东新区中医医院骨伤门诊患者,采用队列研究分析将运用加味麻黄附子细辛汤结合中医综合治疗的腰椎间盘突出症与单纯采用中医综合治疗的腰椎间盘突出症患者分为暴露组和对照组,比较两组患者临床疗效,并将暴露组根据性别、年龄、二十四节气、体质量指数(body mass index,BMI)等因素进一步分亚组比较,探索麻黄附子细辛汤疗效优势人群和使用禁忌。结果共收集204例患者,一般资料分析证实各项数据差异均有可比性。经统计分析,所有亚组均可改善腰椎间盘突出症症状;其中年龄>50岁、BMI<26.9 kg/m^(2)、病程<3年、轻中劳动强度、冬春季组为加味麻黄附子细辛汤的疗效优势人群;回归分析显示,患者年龄越大,加味麻黄附子细辛汤疗效越好;病程越长,加味麻黄附子细辛汤疗效越差;高血压具有双向影响机制。结论①加味麻黄附子细辛汤可改善太阳少阴合病腰椎间盘突出症患者的症状;②加味麻黄附子细辛汤具有特定的疗效优势人群,在临床中,应选择有相应特征的患者使用。展开更多
基金the National Natural Science Foundation of China(81774375)the Fundamental Research Funds for the Central Universities(2018-JYB-ZDSYS002).
文摘Objective:As a traditional herbal formula,Mahuang Xixin Fuzi decoction(MXFD)has been used to treat allergic rhinitis(AR).It regulates the transcription factor GATA3 in T cells,blocks Th2 skewing and rebalances the Th1/Th2 immunity.Type 2 innate lymphoid cells(ILC2s)are closely associated with GATA3.However,it remains unknown whether ILC2s could be one mechanism through which MXFD acts.We sought to elucidate the efficacy and mechanism of action of this decoction in AR treatment.Methods:Forty C57BL/6J female mice were equally divided into control,model,loratadine-and MXFDtreated groups in random.AR was induced by ovalbumin(OVA),and then treatment with loratadine or MXFD was administered.AR scores were evaluated and compared before and after treatment.Pathological changes in the nasal mucosa and lung were observed using hematoxylin-eosin staining of tissue samples.Activation of ILC2 in nasal mucosa was assessed by immunofluorescence and quantitative polymerase chain reaction analysis.ILC2 cell count in lungs was measured by flow cytometry and levels of interleukin-(IL)4,IL-5 and IL-13 in serum were detected by ELISA.Results:The decoction alleviated the symptoms of AR in mice,improved vascular congestion and expansion,glandular hyperplasia and inflammatory cell infiltration in mouse nasal mucosa and slowly improved the interstitial pneumonia and lesions.Meanwhile,MXFD reduced the number and percentage of ILC2s in the nasal mucosa and lungs,downregulated the expression of GATA3 mRNA and RORa mRNA,and reduced the related inflammatory cytokine levels,including IL-4,IL-5 and IL-13.Conclusion:These data suggest that inhibition of ILC2s by MXFD may be an important means by which to treat AR.
文摘目的探索加味麻黄附子细辛汤治疗腰椎间盘突出症疗效优势人群和和使用禁忌,凸显六经辨证特色和优势。方法选取2019年3月~2020年3月就诊于上海市浦东新区中医医院骨伤门诊患者,采用队列研究分析将运用加味麻黄附子细辛汤结合中医综合治疗的腰椎间盘突出症与单纯采用中医综合治疗的腰椎间盘突出症患者分为暴露组和对照组,比较两组患者临床疗效,并将暴露组根据性别、年龄、二十四节气、体质量指数(body mass index,BMI)等因素进一步分亚组比较,探索麻黄附子细辛汤疗效优势人群和使用禁忌。结果共收集204例患者,一般资料分析证实各项数据差异均有可比性。经统计分析,所有亚组均可改善腰椎间盘突出症症状;其中年龄>50岁、BMI<26.9 kg/m^(2)、病程<3年、轻中劳动强度、冬春季组为加味麻黄附子细辛汤的疗效优势人群;回归分析显示,患者年龄越大,加味麻黄附子细辛汤疗效越好;病程越长,加味麻黄附子细辛汤疗效越差;高血压具有双向影响机制。结论①加味麻黄附子细辛汤可改善太阳少阴合病腰椎间盘突出症患者的症状;②加味麻黄附子细辛汤具有特定的疗效优势人群,在临床中,应选择有相应特征的患者使用。
文摘目的探究麻黄细辛附子汤对Th2偏移、Treg细胞功能及Notch信号通路的干预作用。方法体外培养CD4+T细胞,流式细胞仪鉴定纯度,使用白细胞介素-4(IL-4)、干扰素-γ抗体(Anti-IFN-γ)诱导建立Th2细胞分化模型,DAPT及麻黄细辛附子汤干预24 h后收集上清及细胞,ELISA检测白细胞介素-5(IL-5)、干扰素-γ(IFN-γ)、转化生长因子β1(TGF-β1)含量,RT-PCR检测T细胞特异性转录因子(T-bet)、转录因子结合蛋白-3(GATA-3)、叉头翼状螺旋转录因子3(FOXP3)、Notch1 mRNA表达,Western blotting检测T-bet、GATA-3、FOXP3、Notch1蛋白表达。结果1)较之空白组,模型组IL-5含量升高,IFN-γ、TGF-β1含量降低(P<0.01);较之模型组,各给药组IL-5含量降低,IFN-γ、TGF-β1含量升高(P<0.01)。2)较之空白组,模型组Tbet、FOXP3 mRNA表达降低,GATA-3、Notch1 m RNA表达升高(P<0.01);较之模型组,各给药组T-bet、FOXP3 mRNA表达升高,GATA-3、Notch1 m RNA表达降低(P<0.01)。3)较之空白组,模型组T-bet、FOXP3蛋白表达降低,GATA-3、Notch1蛋白表达升高(P<0.01);较之模型组,各给药组T-bet、FOXP3蛋白表达升高,GATA-3、Notch1蛋白表达降低(P<0.01)。结论1)麻黄细辛附子汤能够下调IL-5分泌和GATA-3表达,促进IFN-γ分泌和T-bet表达,纠正Th2偏移。2)麻黄细辛附子汤有助于Treg细胞分化增殖,能够促进TGF-β1分泌和FOXP3表达。3)麻黄细辛附子汤能够通过抑制Notch1表达,促进Treg细胞功能发挥,纠正Th2偏移。
