Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leave...Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leaves were extracted with ethanol and then evaporated.The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H<sub>2</sub>O（50:50,100:0,v/v） and two fractions,Mallotus fiiretianus A（Mf A） and Mallotus fiiretianus B（MfB） were obtained.RaLs were divided into control,atherosclerosis and vitamin E,Mf A and Mf B treated groups.Atherosclerotic model was established by administering u loading dose of vitamin D<sub>3</sub> and feeding standard diet enriched with 2%cholesterol,0.5%porcine chelate,0.2%methimazole,5%sugar,10%pork fat. Vitamin E（0.20 g/kg）,Mf A（0.053 g/kg）,Mf B（0.057 g/kg）（with the potential） were administered to interfere with the development of atherosclerosis.After 9 weeks,rats were sacrificed and the blood lipid as well as composition of bile was examined.In addition,the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.Results: Atherosclerosis model was successfully established,administration of vitamin E,Mf A and Mf B increased excretion of total bilirubin in bile,decreased triglycridc（TO）,total cholesterol（TC）, low density lipoprotein-cholesterol（LDL-C） level,enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood,improved histological changes and diminished intimamedia thickness ratio of thoracic aorta in atherosclerotic rats.As for the difference in antiatherosclerotic effects between Mf A and MfB,Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.Conclusions:The two different extracts. Mf A and Mf B can prevent the development of atherosclerosis.In detail,Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC.level in atherosclerotic rats.展开更多
Objective: To investigate new scolicidal agent from natural resources to cope with the side effects associated with synthetic drugs in Echinococcosis. Methods: The scolicidal potential of methanolic fruit powder extra...Objective: To investigate new scolicidal agent from natural resources to cope with the side effects associated with synthetic drugs in Echinococcosis. Methods: The scolicidal potential of methanolic fruit powder extract (10 and 20 mg/mL) of Mallotus philippinensis ( M. philippinensis ) was investigated. Viability of protoscoleces was confirmed by trypan blue exclusion method, where mortality was observed at concentration of 10 and 20 mg/mL in 60 min treatment against Echinococcus granulosus ( E. granulosus ), under in-vitro conditions with reference to the known standard drug Praziquantel . Results: At concentration 10 and 20 mg/mL, the mortality rate was observed 97% and 99% respectively for 60 min treatment; while up to 93% mortality was observed with 20 mg/mL for only 10 min treatment. The concentration above 20 mg/mL for above 2 h showed 100% mortality, irrespective of further incubation. Conclusions: As compared with the standard anti-parasitic drug Praziquantel our extract has significant scolicidal activity with almost no associated side effects.展开更多
[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assiste...[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.展开更多
[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as indep...[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as independent variables,the content of total flavonoids as dependent variables,the completely secondary response surface regression fitting was conducted on the independent and dependent variables,and the Response Surface Method was used to optimize the optimum extraction process of total flavonoids in Mallotus apelta stems and predict the optimum process. [Results] The optimum extraction process of total flavonoids in Mallotus apelta was determined as follows: ethanol concentration of 71. 5%; extraction time of 154. 6 min; solid-liquid ratio of 1∶19. 2; total flavonoids content of 7. 060 mg/g; fitted binomial squared correlation coefficient R^2= 0. 8751.[Conclusions]Composite Design/Response Surface Method could be used in the extraction process optimization of total flavonoids in Mallotus apelta stems,the mathematical model established had high prediction accuracy,the method was simple and operability was good.展开更多
Objective:To investigate the therapeutic effects of ethyl acetate extract of Mallotus repandus stem in α-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats...Objective:To investigate the therapeutic effects of ethyl acetate extract of Mallotus repandus stem in α-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Ethyl acetate extract of Mallotus repandus stem was tested for the presence of phytochemical constituents,α-amylase inhibitory activity and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.Results:Presence of different types of phytochemicals was identified in the extract.The extract has moderate α-amylase inhibitory activity[IC_(50)=(2.038±0.033)mg/mL]as compared to acarbose.The does 1000 mg/kg significantly reduced(P<0.0100)fasting blood glucose level in normal rats.In oral glucose tolerance test,both 1000 and 2000 mg/kg doses showed good hypoglycemic activity(P<0.0001)like glibenclamide in each specific hour after administration.Overall time effect in oral glucose tolerance test was found extremely significant(P<0.0001)with F(3,48)value=202.4.Conclusions:These findings suggest that this plant may be a potential source for the development of new oral hypoglycemic agent.展开更多
基金supported by Piaopu grant from Hainan Medical College,China(No.200309)
文摘Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leaves were extracted with ethanol and then evaporated.The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H<sub>2</sub>O（50:50,100:0,v/v） and two fractions,Mallotus fiiretianus A（Mf A） and Mallotus fiiretianus B（MfB） were obtained.RaLs were divided into control,atherosclerosis and vitamin E,Mf A and Mf B treated groups.Atherosclerotic model was established by administering u loading dose of vitamin D<sub>3</sub> and feeding standard diet enriched with 2%cholesterol,0.5%porcine chelate,0.2%methimazole,5%sugar,10%pork fat. Vitamin E（0.20 g/kg）,Mf A（0.053 g/kg）,Mf B（0.057 g/kg）（with the potential） were administered to interfere with the development of atherosclerosis.After 9 weeks,rats were sacrificed and the blood lipid as well as composition of bile was examined.In addition,the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.Results: Atherosclerosis model was successfully established,administration of vitamin E,Mf A and Mf B increased excretion of total bilirubin in bile,decreased triglycridc（TO）,total cholesterol（TC）, low density lipoprotein-cholesterol（LDL-C） level,enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood,improved histological changes and diminished intimamedia thickness ratio of thoracic aorta in atherosclerotic rats.As for the difference in antiatherosclerotic effects between Mf A and MfB,Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.Conclusions:The two different extracts. Mf A and Mf B can prevent the development of atherosclerosis.In detail,Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC.level in atherosclerotic rats.
基金support provided by Department of Science and Technology,Government of India, New Delhi, in the form of project grant(vide file no.SR/SO/HS-0062/2009)
文摘Objective: To investigate new scolicidal agent from natural resources to cope with the side effects associated with synthetic drugs in Echinococcosis. Methods: The scolicidal potential of methanolic fruit powder extract (10 and 20 mg/mL) of Mallotus philippinensis ( M. philippinensis ) was investigated. Viability of protoscoleces was confirmed by trypan blue exclusion method, where mortality was observed at concentration of 10 and 20 mg/mL in 60 min treatment against Echinococcus granulosus ( E. granulosus ), under in-vitro conditions with reference to the known standard drug Praziquantel . Results: At concentration 10 and 20 mg/mL, the mortality rate was observed 97% and 99% respectively for 60 min treatment; while up to 93% mortality was observed with 20 mg/mL for only 10 min treatment. The concentration above 20 mg/mL for above 2 h showed 100% mortality, irrespective of further incubation. Conclusions: As compared with the standard anti-parasitic drug Praziquantel our extract has significant scolicidal activity with almost no associated side effects.
基金Supported by the Program of Guilin Municipal Science and Technology Bureau(20130403-4)Science and Technology Program of Traditional Chinese Medicine from Guangxi Administration of Traditional Chinese Medicine(GZLC14-31)Undergraduate Innovation and Entrepreneurship Training Plan Project of Autonomous District Level in 2017(201710601082)
文摘[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.
基金Supported by Chinese Medicine Science and Technology Project of Guangxi Administration of Traditional Chinese Medicine(GZLC14-31)Science and Technology Research and Development Program of Guilin Bureau of Technology(20130403-4)+1 种基金Guangxi"2011 Collaborative Innovation Center"-Zhuang and Yao Medicine Collaborative Innovation Center(Gui201320)the Autonomous Region-Level College Students’ Innovation and Entrepreneurship Training Program(201710601082)
文摘[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as independent variables,the content of total flavonoids as dependent variables,the completely secondary response surface regression fitting was conducted on the independent and dependent variables,and the Response Surface Method was used to optimize the optimum extraction process of total flavonoids in Mallotus apelta stems and predict the optimum process. [Results] The optimum extraction process of total flavonoids in Mallotus apelta was determined as follows: ethanol concentration of 71. 5%; extraction time of 154. 6 min; solid-liquid ratio of 1∶19. 2; total flavonoids content of 7. 060 mg/g; fitted binomial squared correlation coefficient R^2= 0. 8751.[Conclusions]Composite Design/Response Surface Method could be used in the extraction process optimization of total flavonoids in Mallotus apelta stems,the mathematical model established had high prediction accuracy,the method was simple and operability was good.
基金Supported by Jahangirnagar University Annual Research Grant(Grant No.JU-ARG-2012-005).
文摘Objective:To investigate the therapeutic effects of ethyl acetate extract of Mallotus repandus stem in α-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Ethyl acetate extract of Mallotus repandus stem was tested for the presence of phytochemical constituents,α-amylase inhibitory activity and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.Results:Presence of different types of phytochemicals was identified in the extract.The extract has moderate α-amylase inhibitory activity[IC_(50)=(2.038±0.033)mg/mL]as compared to acarbose.The does 1000 mg/kg significantly reduced(P<0.0100)fasting blood glucose level in normal rats.In oral glucose tolerance test,both 1000 and 2000 mg/kg doses showed good hypoglycemic activity(P<0.0001)like glibenclamide in each specific hour after administration.Overall time effect in oral glucose tolerance test was found extremely significant(P<0.0001)with F(3,48)value=202.4.Conclusions:These findings suggest that this plant may be a potential source for the development of new oral hypoglycemic agent.
