To study the effect of manganese exposure on the herbivorous insect Lymantria dispar asiatica,fourthinstar larvae were fed a MnCl_(2)-amended diet(LdMn)for 84 h(0.40 mmol MnCl_(2)/g diet).Larvae were weighed before an...To study the effect of manganese exposure on the herbivorous insect Lymantria dispar asiatica,fourthinstar larvae were fed a MnCl_(2)-amended diet(LdMn)for 84 h(0.40 mmol MnCl_(2)/g diet).Larvae were weighed before and after the diet administration to assess larval gain in mass under manganese exposure.The whole bodies of half of the survivors were ground in liquid nitrogen for measuring enzyme activities and total antioxidant capacity(T-AOC).The intestinal tracts of the remaining survivors were collected and immediately frozen in liquid nitrogen for 16S rDNA sequencing of gut microbiota.Larvae under manganese stress lost significant mass(p<0.05).The activities of digestive and antioxidant enzymes and T-AOC,but not trehalase and polyphenol oxidase,were significantly higher after Mn exposure,(p<0.05).A Venn diagram illustrated that the gut microbial OTU composition in the larvae also changed.Community pies and correlation heatmaps also showed different relative abundances of gut microbes.In other words,species quantity and relative abundance of gut microbes agreed with PCoA visualization and indicated that the gut microbial community in L.dispar asiatica larvae differed significantly between control and LdMn.Functional classification also suggested that exposure to manganese stress significantly decreased gut microbial coenzyme transport and metabolism in L.dispar asiatica larvae.These results further our understanding about stress response of L.dispar asiatica larvae.展开更多
AIM:To evaluate the clinical significance of oxidative stress markers in patients with hepatitis C virus(HCV)related hepatocellular carcinoma(HCC).METHODS:Sixty-four consecutive patients who were admitted to Kagoshima...AIM:To evaluate the clinical significance of oxidative stress markers in patients with hepatitis C virus(HCV)related hepatocellular carcinoma(HCC).METHODS:Sixty-four consecutive patients who were admitted to Kagoshima University Medical and Dental Hospital were enrolled in this retrospective study.All patients had chronic liver disease(CLD) due to infection with HCV.Thirty patients with HCV-related HCC,34 with HCV-related CLD without HCC(non-HCC),and 20 healthy volunteers(HVs) were enrolled.Possible associations between serum manganese superoxide dismutase(MnSOD) and thioredoxin(TRX) levels and clinical parameters or patient prognosis were analyzed over a mean follow-up period of 31.7 mo.RESULTS:The serum MnSOD levels were significantly higher in patients with HCV-related HCC than in patients without HCC(P = 0.03) or HVs(P < 0.001).Similarly,serum TRX levels were also significantly higher in patients with HCV-related HCC than in patients without HCC(P = 0.04) or HVs(P < 0.01).However,serum levels of MnSOD and TRX were not correlated in patients with HCC.Among patients with HCC,the overall survival rate(OSR) was lower in patients with MnSOD levels ≥ 110 ng/mL than in patients with levels < 110 ng/mL(P = 0.01),and the OSR tended to be lower in patients with TRX levels < 80 ng/mL(P = 0.05).In addition,patient prognosis with HCC was poorest with serum MnSOD levels ≥ 110 ng/mL and serum TRX levels < 80 ng/mL.Furthermore,a multivariate analysis using a Cox proportional hazard model and serum levels of five factors(MnSOD,prothrombin time,serum albumin,serum α-fetoprotein(AFP),and serum des-γ-carboxy prothrombin) revealed that MnSOD levels ≥ 110 ng/mL(risk ratio:4.12,95% confidential interval:1.22-13.88,P = 0.02) and AFP levels ≥ 40 ng/mL(risk ratio:6.75;95% confidential interval:1.70-26.85,P < 0.01) were independent risk factors associated with a poor patient prognosis.CONCLUSION:Serum MnSOD and TRX levels are potential clinical biomarkers that predict patient prognosis in HCV-related HCC.展开更多
基金funded partly by the Fundamental Research Funds of the Chinese Central Universities(grant no.2572017AA18)Chinese Central Financial Forest Science and Technology Extension Demonstration Fund Project(grant no.JLT[2016]13)。
文摘To study the effect of manganese exposure on the herbivorous insect Lymantria dispar asiatica,fourthinstar larvae were fed a MnCl_(2)-amended diet(LdMn)for 84 h(0.40 mmol MnCl_(2)/g diet).Larvae were weighed before and after the diet administration to assess larval gain in mass under manganese exposure.The whole bodies of half of the survivors were ground in liquid nitrogen for measuring enzyme activities and total antioxidant capacity(T-AOC).The intestinal tracts of the remaining survivors were collected and immediately frozen in liquid nitrogen for 16S rDNA sequencing of gut microbiota.Larvae under manganese stress lost significant mass(p<0.05).The activities of digestive and antioxidant enzymes and T-AOC,but not trehalase and polyphenol oxidase,were significantly higher after Mn exposure,(p<0.05).A Venn diagram illustrated that the gut microbial OTU composition in the larvae also changed.Community pies and correlation heatmaps also showed different relative abundances of gut microbes.In other words,species quantity and relative abundance of gut microbes agreed with PCoA visualization and indicated that the gut microbial community in L.dispar asiatica larvae differed significantly between control and LdMn.Functional classification also suggested that exposure to manganese stress significantly decreased gut microbial coenzyme transport and metabolism in L.dispar asiatica larvae.These results further our understanding about stress response of L.dispar asiatica larvae.
基金Supported by (in part) Grants from the Ministry of Education,Culture, Sports, Science and Technology of Japan, and the Ministry of Health, Labour and Welfare of Japan
文摘AIM:To evaluate the clinical significance of oxidative stress markers in patients with hepatitis C virus(HCV)related hepatocellular carcinoma(HCC).METHODS:Sixty-four consecutive patients who were admitted to Kagoshima University Medical and Dental Hospital were enrolled in this retrospective study.All patients had chronic liver disease(CLD) due to infection with HCV.Thirty patients with HCV-related HCC,34 with HCV-related CLD without HCC(non-HCC),and 20 healthy volunteers(HVs) were enrolled.Possible associations between serum manganese superoxide dismutase(MnSOD) and thioredoxin(TRX) levels and clinical parameters or patient prognosis were analyzed over a mean follow-up period of 31.7 mo.RESULTS:The serum MnSOD levels were significantly higher in patients with HCV-related HCC than in patients without HCC(P = 0.03) or HVs(P < 0.001).Similarly,serum TRX levels were also significantly higher in patients with HCV-related HCC than in patients without HCC(P = 0.04) or HVs(P < 0.01).However,serum levels of MnSOD and TRX were not correlated in patients with HCC.Among patients with HCC,the overall survival rate(OSR) was lower in patients with MnSOD levels ≥ 110 ng/mL than in patients with levels < 110 ng/mL(P = 0.01),and the OSR tended to be lower in patients with TRX levels < 80 ng/mL(P = 0.05).In addition,patient prognosis with HCC was poorest with serum MnSOD levels ≥ 110 ng/mL and serum TRX levels < 80 ng/mL.Furthermore,a multivariate analysis using a Cox proportional hazard model and serum levels of five factors(MnSOD,prothrombin time,serum albumin,serum α-fetoprotein(AFP),and serum des-γ-carboxy prothrombin) revealed that MnSOD levels ≥ 110 ng/mL(risk ratio:4.12,95% confidential interval:1.22-13.88,P = 0.02) and AFP levels ≥ 40 ng/mL(risk ratio:6.75;95% confidential interval:1.70-26.85,P < 0.01) were independent risk factors associated with a poor patient prognosis.CONCLUSION:Serum MnSOD and TRX levels are potential clinical biomarkers that predict patient prognosis in HCV-related HCC.
