Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of C.pilosula;nonetheless,until now,no validated ...Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of C.pilosula;nonetheless,until now,no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction(RT–qPCR)data.Methods:To screen stable reference genes of S.ureilytica DW2,the expression of its eight candidate reference genes(16S rRNA,ftsZ,ftsA,mreB,recA,slyD,thiC,and zipA)under different treatment conditions(pH,temperature,culture time,and salt content)was assayed by RT–qPCR.The expression stability of these genes was analyzed using different algorithms(geNorm,NormFinder,and BestKeeper).To verify the reliability of the data,the expression of the glucose dehydrogenase(gdh)gene under different soluble phosphate levels was quantified using the most stably expressed reference gene.Results:The results showed that the zipA and 16S rRNA genes were the most stable reference genes,and the least stable genes were thiC and recA.The expression of gdh was consistent with the phosphate solubilization ability on plates containing the National Botanical Research Institute phosphate growth medium.Conclusion:Therefore,this study provides a stable and reliable reference gene of Serratia for the accurate quantification of functional gene expression in future studies.展开更多
Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%d.b.Increasing of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55...Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%d.b.Increasing of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55 mm),thickness(0.97-1.05 mm),arithmetic mean diameter(2.48-2.73),geometric mean diameter(1.88-2.09 mm),surface area(10.34-12.66 mm^(2)),thousand seed weight(2.9-3.9 g),porosity(51.22%-64.11%),true density(917.8-1030.6 kg/m3),static angle of repose(43-49 deg),dynamic angle of repose(47-56.6 deg),and coefficient of static friction on the three surfaces:glass(0.48-0.77),galvanized iron sheet(0.36-0.73),and plywood(0.57-0.69).However,bulk density was found to decrease from 447.66-369.88 kg/m3,and rupture force and rupture energy along with seed length and width were found to decrease from 83.74-56.17 N,132.95-84.47 N,50.66-27.52 mJ,and 67.8 to 33.36 mJ,respectively.The sphericity increased from 36.63%to 37.5%with increasing in moisture content from 7.24%to 14.5%d.b.and then reduced to 37.5%with further increase in moisture content to 21.38%d.b.展开更多
基金supported by the General Program of Natural Science Foundation of China(32071770)the Fundamental Research Program of Shanxi Province(Award No.202103021223380)the Fund for Shanxi“1331 Project”Key Subjects Construction(1331KSC).
文摘Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of C.pilosula;nonetheless,until now,no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction(RT–qPCR)data.Methods:To screen stable reference genes of S.ureilytica DW2,the expression of its eight candidate reference genes(16S rRNA,ftsZ,ftsA,mreB,recA,slyD,thiC,and zipA)under different treatment conditions(pH,temperature,culture time,and salt content)was assayed by RT–qPCR.The expression stability of these genes was analyzed using different algorithms(geNorm,NormFinder,and BestKeeper).To verify the reliability of the data,the expression of the glucose dehydrogenase(gdh)gene under different soluble phosphate levels was quantified using the most stably expressed reference gene.Results:The results showed that the zipA and 16S rRNA genes were the most stable reference genes,and the least stable genes were thiC and recA.The expression of gdh was consistent with the phosphate solubilization ability on plates containing the National Botanical Research Institute phosphate growth medium.Conclusion:Therefore,this study provides a stable and reliable reference gene of Serratia for the accurate quantification of functional gene expression in future studies.
文摘Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%d.b.Increasing of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55 mm),thickness(0.97-1.05 mm),arithmetic mean diameter(2.48-2.73),geometric mean diameter(1.88-2.09 mm),surface area(10.34-12.66 mm^(2)),thousand seed weight(2.9-3.9 g),porosity(51.22%-64.11%),true density(917.8-1030.6 kg/m3),static angle of repose(43-49 deg),dynamic angle of repose(47-56.6 deg),and coefficient of static friction on the three surfaces:glass(0.48-0.77),galvanized iron sheet(0.36-0.73),and plywood(0.57-0.69).However,bulk density was found to decrease from 447.66-369.88 kg/m3,and rupture force and rupture energy along with seed length and width were found to decrease from 83.74-56.17 N,132.95-84.47 N,50.66-27.52 mJ,and 67.8 to 33.36 mJ,respectively.The sphericity increased from 36.63%to 37.5%with increasing in moisture content from 7.24%to 14.5%d.b.and then reduced to 37.5%with further increase in moisture content to 21.38%d.b.
