Melia azedarach L. extracts were studied in comparison with selected synthetic in-secticides against the mulberry whiteflies Pealiusma chili and P. mori under labora-tory conditions. Morus alba (cv. “Early Spring”) ...Melia azedarach L. extracts were studied in comparison with selected synthetic in-secticides against the mulberry whiteflies Pealiusma chili and P. mori under labora-tory conditions. Morus alba (cv. “Early Spring”) leaves infested by mulberry white-flies were exposed to Melia extracts of green fruits, l-cyhalothrin (10% a.i.) and con-trol. Also, white mulberry leaves after treatment with the same insecticides were provided to silkworms for consumption. Results have shown that there were signif-icant differences in the nymphal mortality of the whiteflies among treatments. Melia extracts and l-cyhalothrin were effective in increasing whitefly mortality 24 h after application. Furthermore, Melia extracts didn’t affect silkworm survivorship, which was severely affected by l-cyhalothrin treatment, since all the silkworms fed on treated leaves were found dead. Thus, these extracts might be considered a potential alternative in management of the mulberry whiteflies.展开更多
Two new triterpenoids(1 and 2)and a new sterol(3),together with six known constituents(4–9),were isolated from the leaves and twigs of Melia azedarach.Their chemical structures were elucidated on the basis of spectro...Two new triterpenoids(1 and 2)and a new sterol(3),together with six known constituents(4–9),were isolated from the leaves and twigs of Melia azedarach.Their chemical structures were elucidated on the basis of spectroscopic analysis.展开更多
Objective:To investigate the antibacterial potential of the polar and non-polar extracts of the seeds of Melia azedarach(M.azedarach) L.(Meliaceae) against eighteen hospital isolated human pathogenic bacterial strains...Objective:To investigate the antibacterial potential of the polar and non-polar extracts of the seeds of Melia azedarach(M.azedarach) L.(Meliaceae) against eighteen hospital isolated human pathogenic bacterial strains.Methods:Petrol,benzene,ethyl acetate,methanol,and aqueous extracts at five different concentrations(1,2,5,10 and 15 mg/mL) were evaluated.Disk diffusion method was followed to evaluate the antibacterial efficacy.Results:All extracts of the seeds demonstrated significant antibacterial activity against tested pathogens.Among all extracts,ethyl acetate extract revealed the highest inhibition comparatively.The present study also favored the traditional uses reported earlier.Conclusions:Results of this study strongly confirm that the seed extracts of M.azedarach could be effective antibiotics,both in controlling gram-positive and gram-negative human pathogenic infections.展开更多
Objective: To evaluate the anti-mycobacterial activity of Melia azedarach L.(M. azedarach) and Lobelia chinensis Lour.(L. chinensis) extracts against the growth of Mycobacterium tuberculosis(M. tuberculosis).Methods: ...Objective: To evaluate the anti-mycobacterial activity of Melia azedarach L.(M. azedarach) and Lobelia chinensis Lour.(L. chinensis) extracts against the growth of Mycobacterium tuberculosis(M. tuberculosis).Methods: The anti-M. tuberculosis activity of M. azedarach and L. chinensis extracts were evaluated using different indicator methods such as resazurin microtiter assay(REMA) and mycobacteria growth indicator tube(MGIT) 960 system assay. The M. tuberculosis was incubated with various concentrations(50–800 mg/m L) of the extracts for 5 days in the REMA, and for 4 weeks in MGIT 960 system assay.Results: M. azedarach and L. chinensis extracts showed their anti-M. tuberculosis activity by strongly inhibiting the growth of M. tuberculosis in a concentration-dependent manner in the REMA and the MGIT 960 system assay. Particularly, the methanol extract of M. azedarach and n-hexane extract of L. chinensis consistently exhibited their effects by effectively inhibiting the growth of M. tuberculosis in MGIT 960 system for 4 weeks with a single-treatment, indicating higher anti-M. tuberculosis activity than other extracts,and their minimum inhibitory concentrations were measured as 400 mg/m L and 800 mg/m L, respectively.Conclusions: These results demonstrate that M. azedarach and L. chinensis extracts not only have unique anti-M. tuberculosis activity, but also induce the selective antiM. tuberculosis effects by consistently inhibiting or blocking the growth of M. tuberculosis through a new pharmacological action. Therefore, this study suggests the potential of them as effective candidate agents of next-generation for developing a new anti-tuberculosis drug, as well as the advantage for utilizing traditional medicinal plants as one of effective strategies against tuberculosis.展开更多
Objective:To assess ethanolic extract of the leaves of Melia azedarach L.(Family-Meliaceae)for its possible antioxidant,analgesic and antibacterial activities in association with performing phytochemical evaluation.Me...Objective:To assess ethanolic extract of the leaves of Melia azedarach L.(Family-Meliaceae)for its possible antioxidant,analgesic and antibacterial activities in association with performing phytochemical evaluation.Methods:A number of phytochemical tests of this extract were utilized to distinguish the existence of different sorts of compounds.Qualitative and quantitative antioxidant activities were assessed by TLC and DPPH scavenging assay respectively.Acetic acid induced writhing test in mice and disk diffusion assay of the leaves extract were carried out to demonstrate the analgesic and antibacterial activities respectively.Results:The phytochemical assessment revealed the existence of alkaloids,flavonoids,glycosides,saponins and tannins like compounds.Ethanolic extract of the leaves demonstrated antioxidant,analgesic and antibacterial activities in vitro.Conclusion:Considering the study,this could justify the leaves extract’s bioactivities but,to substantiate the activity of individual compound further investigation is necessary.展开更多
Two new furan fragment isomerized limonoids, meliazedalides A and B(compounds 1 and 2), were isolated from the fruits of Melia azedarach Linn.. Their chemical structures were elucidated on the basis of HR-ESI-MS and 1...Two new furan fragment isomerized limonoids, meliazedalides A and B(compounds 1 and 2), were isolated from the fruits of Melia azedarach Linn.. Their chemical structures were elucidated on the basis of HR-ESI-MS and 1D and 2D NMR data, which belonged to nimbolinin-and trichilin-class, respectively. Compound 2 exhibited weak inhibitory effect on NO production in lipopolysaccharide(LPS)-activated RAW 264.7 macrophages with IC_(50) being 37.41 μmol·L^(–1).展开更多
文摘Melia azedarach L. extracts were studied in comparison with selected synthetic in-secticides against the mulberry whiteflies Pealiusma chili and P. mori under labora-tory conditions. Morus alba (cv. “Early Spring”) leaves infested by mulberry white-flies were exposed to Melia extracts of green fruits, l-cyhalothrin (10% a.i.) and con-trol. Also, white mulberry leaves after treatment with the same insecticides were provided to silkworms for consumption. Results have shown that there were signif-icant differences in the nymphal mortality of the whiteflies among treatments. Melia extracts and l-cyhalothrin were effective in increasing whitefly mortality 24 h after application. Furthermore, Melia extracts didn’t affect silkworm survivorship, which was severely affected by l-cyhalothrin treatment, since all the silkworms fed on treated leaves were found dead. Thus, these extracts might be considered a potential alternative in management of the mulberry whiteflies.
基金Joint Fund of NSFC and NSFY(No.U1132604)Key Program of MOST of CHINA(Nos.2007BAD32B03 and SB2007FY400)Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2010-ZZ14).
文摘Two new triterpenoids(1 and 2)and a new sterol(3),together with six known constituents(4–9),were isolated from the leaves and twigs of Melia azedarach.Their chemical structures were elucidated on the basis of spectroscopic analysis.
基金Supported by SERC Fast Track OYS Schem,Department of Scienceand Technology(DST).New Delhi,India[No.304/2004(SR/FT/L-129/2004)]
文摘Objective:To investigate the antibacterial potential of the polar and non-polar extracts of the seeds of Melia azedarach(M.azedarach) L.(Meliaceae) against eighteen hospital isolated human pathogenic bacterial strains.Methods:Petrol,benzene,ethyl acetate,methanol,and aqueous extracts at five different concentrations(1,2,5,10 and 15 mg/mL) were evaluated.Disk diffusion method was followed to evaluate the antibacterial efficacy.Results:All extracts of the seeds demonstrated significant antibacterial activity against tested pathogens.Among all extracts,ethyl acetate extract revealed the highest inhibition comparatively.The present study also favored the traditional uses reported earlier.Conclusions:Results of this study strongly confirm that the seed extracts of M.azedarach could be effective antibiotics,both in controlling gram-positive and gram-negative human pathogenic infections.
基金supported by C&K pharmaceutical company in Korea
文摘Objective: To evaluate the anti-mycobacterial activity of Melia azedarach L.(M. azedarach) and Lobelia chinensis Lour.(L. chinensis) extracts against the growth of Mycobacterium tuberculosis(M. tuberculosis).Methods: The anti-M. tuberculosis activity of M. azedarach and L. chinensis extracts were evaluated using different indicator methods such as resazurin microtiter assay(REMA) and mycobacteria growth indicator tube(MGIT) 960 system assay. The M. tuberculosis was incubated with various concentrations(50–800 mg/m L) of the extracts for 5 days in the REMA, and for 4 weeks in MGIT 960 system assay.Results: M. azedarach and L. chinensis extracts showed their anti-M. tuberculosis activity by strongly inhibiting the growth of M. tuberculosis in a concentration-dependent manner in the REMA and the MGIT 960 system assay. Particularly, the methanol extract of M. azedarach and n-hexane extract of L. chinensis consistently exhibited their effects by effectively inhibiting the growth of M. tuberculosis in MGIT 960 system for 4 weeks with a single-treatment, indicating higher anti-M. tuberculosis activity than other extracts,and their minimum inhibitory concentrations were measured as 400 mg/m L and 800 mg/m L, respectively.Conclusions: These results demonstrate that M. azedarach and L. chinensis extracts not only have unique anti-M. tuberculosis activity, but also induce the selective antiM. tuberculosis effects by consistently inhibiting or blocking the growth of M. tuberculosis through a new pharmacological action. Therefore, this study suggests the potential of them as effective candidate agents of next-generation for developing a new anti-tuberculosis drug, as well as the advantage for utilizing traditional medicinal plants as one of effective strategies against tuberculosis.
基金the authorities of Pharmacy Discipline,Life Science School,Khulna University for supporting fund(Grant No.KU:PHRM:2011/April-071112).
文摘Objective:To assess ethanolic extract of the leaves of Melia azedarach L.(Family-Meliaceae)for its possible antioxidant,analgesic and antibacterial activities in association with performing phytochemical evaluation.Methods:A number of phytochemical tests of this extract were utilized to distinguish the existence of different sorts of compounds.Qualitative and quantitative antioxidant activities were assessed by TLC and DPPH scavenging assay respectively.Acetic acid induced writhing test in mice and disk diffusion assay of the leaves extract were carried out to demonstrate the analgesic and antibacterial activities respectively.Results:The phytochemical assessment revealed the existence of alkaloids,flavonoids,glycosides,saponins and tannins like compounds.Ethanolic extract of the leaves demonstrated antioxidant,analgesic and antibacterial activities in vitro.Conclusion:Considering the study,this could justify the leaves extract’s bioactivities but,to substantiate the activity of individual compound further investigation is necessary.
基金supported by the National Natural Science Foundation of China(No.81573550)the Outstanding Youth Fund of the Basic Research Program of Jiangsu Province(BK20160077)
文摘Two new furan fragment isomerized limonoids, meliazedalides A and B(compounds 1 and 2), were isolated from the fruits of Melia azedarach Linn.. Their chemical structures were elucidated on the basis of HR-ESI-MS and 1D and 2D NMR data, which belonged to nimbolinin-and trichilin-class, respectively. Compound 2 exhibited weak inhibitory effect on NO production in lipopolysaccharide(LPS)-activated RAW 264.7 macrophages with IC_(50) being 37.41 μmol·L^(–1).
