In the present study, we developed a novel open-tubular capillary dectrochromatographic method using avidin-phospholipid vesicle complex as the stationary phase for chiral separation of mexiletine hydrochloride. The a...In the present study, we developed a novel open-tubular capillary dectrochromatographic method using avidin-phospholipid vesicle complex as the stationary phase for chiral separation of mexiletine hydrochloride. The avidin immobilized on the phospholipid vesicle consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine （DPPE） and L-u-phosphatidyl-L-serine （PS） （80：20, mg%） was coated in the capillary. The homogeneity and separation performance of the coating were evaluated in terms o f phospholipid vesicle characterization and the resolution of D,L-Tryptophan. As for mexiletine hydrochloride, four vital parameters affecting the separation efficiency of coating capillary, including buffer type, buffer pH, buffer concentration and the applied voltage, were studied in detail. Under the optimum conditions, the enantiomers could be separated well with good resolution. All the satisfactory results indicated that this method using avidin-phospholipid vesicle complex as the stationary phase was suitable and feasible, which had great potential in pharmaceutical separation Of enantiomers.展开更多
基金support of Professor Xuan Zhang’s research group,Laboratory of pharmaceutics,School of Pharmaceutical Sciences,Peking UniversityBeijing Kaiao Technology Development Co.,Ltd.
文摘In the present study, we developed a novel open-tubular capillary dectrochromatographic method using avidin-phospholipid vesicle complex as the stationary phase for chiral separation of mexiletine hydrochloride. The avidin immobilized on the phospholipid vesicle consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine （DPPE） and L-u-phosphatidyl-L-serine （PS） （80：20, mg%） was coated in the capillary. The homogeneity and separation performance of the coating were evaluated in terms o f phospholipid vesicle characterization and the resolution of D,L-Tryptophan. As for mexiletine hydrochloride, four vital parameters affecting the separation efficiency of coating capillary, including buffer type, buffer pH, buffer concentration and the applied voltage, were studied in detail. Under the optimum conditions, the enantiomers could be separated well with good resolution. All the satisfactory results indicated that this method using avidin-phospholipid vesicle complex as the stationary phase was suitable and feasible, which had great potential in pharmaceutical separation Of enantiomers.
