Traditional detection of thiodiglycol(TDG),a metabolic marker for sulfur mustard poisoning,requires not only professional operators,but also expensive reagents and large instruments.Herein,we developed a novel molecul...Traditional detection of thiodiglycol(TDG),a metabolic marker for sulfur mustard poisoning,requires not only professional operators,but also expensive reagents and large instruments.Herein,we developed a novel molecular imprinted polymers(MIPs)-based lateral flow assay(LFA)strategy for the quick,sensitive,and selective detection of TDG.Gold nanoparticles(Au NPs),MIPs,and metallothioneins(MTs)were respectively loaded on the conjugate pad,test line(T line)and control line(C line).After adding TDG,Au NPs on the conjugate pad reacted with TDG through the Au-S bond first.Then,under the action of capillary force,the conjugates of TDG and Au NPs were trapped by the MIPs as they traveled to the T line,and the residual Au NPs bound with the MTs on the C line,exhibiting two obvious red bands on T line and C line,respectively.In contrast,a single red band could be observed on C line without TDG.This method exhibited a wide linear range from 10.0 pg/m L to 10,000.0 ng/m L and its limit of detection(LOD)was as low as 0.41 pg/m L.This method was successfully utilized to detect TDG in human urine,presenting significant potential in the point-of-care testing of TDG in clinical samples of the sulfur mustard poisoning patients.展开更多
The interaction process between the phenobarbital(PHN) and acrylamide(AM) was studied using the M062X/6-31G(d,p) method. The PHN and AM were used as the template and functional monomer,respectively. The molecular elec...The interaction process between the phenobarbital(PHN) and acrylamide(AM) was studied using the M062X/6-31G(d,p) method. The PHN and AM were used as the template and functional monomer,respectively. The molecular electrostatic potential(MEP) was simulated for predicting the reactive sites. The atoms in molecules theory helped to reveal the imprinting mechanism and optimize the molar ratios for PHN and AM. The molecular imprinted polymers(MIPs) containing PHN were synthesized through the precipitation polymerization. The diameter range of the obtained MIPs was from 150 to 390 nm. According to the computational results,MIPs with the molar ratio of PHN and AM equal to 1:6 showed high selective adsorption for PHN. The apparent maximum adsorption quantity(Q_(max)) of MIPs toward PHN was 7.9 mg/g,and the Qmax of nonimprinted polymer microspheres(NIPs) was 3.2 mg/g. Herein,the studies can provide theoretical and experimental references for the controllable fabrication of MIPs.展开更多
Hollow molecular imprinted polymer microspheres were prepared by distillation precipitation polymerization with(S)-(+)-ibuprofen(S-IBF)as template molecule and acrylamide(AM)as functional monomer.Using the silicon dio...Hollow molecular imprinted polymer microspheres were prepared by distillation precipitation polymerization with(S)-(+)-ibuprofen(S-IBF)as template molecule and acrylamide(AM)as functional monomer.Using the silicon dioxide(SiO_(2),180 nm)modified by 3-(trimethoxysilyl)propyl methacrylate(MPS)as the template micro-spheres,the molecular imprinted shells were coated on successfully(SiO_(2)@MIPs).The thermosensitive SiO_(2)@MIPs-PNIPAM core-shell microspheres were subsequently prepared by grafting the PNIPAM chains(M_(n)=1.21×10^(4)g/mol,polydispersity index=1.30),which were prepared by reversible addition-fragmentation chain transfer(RAFT)polymerization,on the surface of SiO_(2)@MIPs microspheres via the thiol-ene click chemistry.The grafting density of PNIPAM brushes on the SiO_(2)@MIPs microspheres was about 0.18 chains/nm^(2).After HF etching,the hollow imprinted microspheres were finally obtained.For thermosensitivity analysis,the phase transition temperatures of multifunctional nanoparticles were measured by DSL at 25℃and 45℃respectively,and the sizes of the microspheres changed by about 35 nm.The modified microspheres presented excellent controlled release property to S-IBF,moreover about half amount of the adsorptions passed into acetonitrile-water solution through the specific holes of imprinted shell at 25℃under vibration.展开更多
基金supported by the National Key Research and Development Program of China(2018YFC1602600)the National Natural Science Foundation of China(21974109)the Natural Science Foundation of Chongqing(CSTB2022NSCQ-MSX1662)
文摘Traditional detection of thiodiglycol(TDG),a metabolic marker for sulfur mustard poisoning,requires not only professional operators,but also expensive reagents and large instruments.Herein,we developed a novel molecular imprinted polymers(MIPs)-based lateral flow assay(LFA)strategy for the quick,sensitive,and selective detection of TDG.Gold nanoparticles(Au NPs),MIPs,and metallothioneins(MTs)were respectively loaded on the conjugate pad,test line(T line)and control line(C line).After adding TDG,Au NPs on the conjugate pad reacted with TDG through the Au-S bond first.Then,under the action of capillary force,the conjugates of TDG and Au NPs were trapped by the MIPs as they traveled to the T line,and the residual Au NPs bound with the MTs on the C line,exhibiting two obvious red bands on T line and C line,respectively.In contrast,a single red band could be observed on C line without TDG.This method exhibited a wide linear range from 10.0 pg/m L to 10,000.0 ng/m L and its limit of detection(LOD)was as low as 0.41 pg/m L.This method was successfully utilized to detect TDG in human urine,presenting significant potential in the point-of-care testing of TDG in clinical samples of the sulfur mustard poisoning patients.
基金Supported by the Science and Technology Development Program of Jilin Province(Nos.20150101018JC and 20130206099SF)the National Natural Science Foundation of China(Nos.21302062 and 21563002)
文摘The interaction process between the phenobarbital(PHN) and acrylamide(AM) was studied using the M062X/6-31G(d,p) method. The PHN and AM were used as the template and functional monomer,respectively. The molecular electrostatic potential(MEP) was simulated for predicting the reactive sites. The atoms in molecules theory helped to reveal the imprinting mechanism and optimize the molar ratios for PHN and AM. The molecular imprinted polymers(MIPs) containing PHN were synthesized through the precipitation polymerization. The diameter range of the obtained MIPs was from 150 to 390 nm. According to the computational results,MIPs with the molar ratio of PHN and AM equal to 1:6 showed high selective adsorption for PHN. The apparent maximum adsorption quantity(Q_(max)) of MIPs toward PHN was 7.9 mg/g,and the Qmax of nonimprinted polymer microspheres(NIPs) was 3.2 mg/g. Herein,the studies can provide theoretical and experimental references for the controllable fabrication of MIPs.
基金supported by the Tianjin Natural Science Funds of China(No.13JCQNJC02600).
文摘Hollow molecular imprinted polymer microspheres were prepared by distillation precipitation polymerization with(S)-(+)-ibuprofen(S-IBF)as template molecule and acrylamide(AM)as functional monomer.Using the silicon dioxide(SiO_(2),180 nm)modified by 3-(trimethoxysilyl)propyl methacrylate(MPS)as the template micro-spheres,the molecular imprinted shells were coated on successfully(SiO_(2)@MIPs).The thermosensitive SiO_(2)@MIPs-PNIPAM core-shell microspheres were subsequently prepared by grafting the PNIPAM chains(M_(n)=1.21×10^(4)g/mol,polydispersity index=1.30),which were prepared by reversible addition-fragmentation chain transfer(RAFT)polymerization,on the surface of SiO_(2)@MIPs microspheres via the thiol-ene click chemistry.The grafting density of PNIPAM brushes on the SiO_(2)@MIPs microspheres was about 0.18 chains/nm^(2).After HF etching,the hollow imprinted microspheres were finally obtained.For thermosensitivity analysis,the phase transition temperatures of multifunctional nanoparticles were measured by DSL at 25℃and 45℃respectively,and the sizes of the microspheres changed by about 35 nm.The modified microspheres presented excellent controlled release property to S-IBF,moreover about half amount of the adsorptions passed into acetonitrile-water solution through the specific holes of imprinted shell at 25℃under vibration.
