BACKGROUND Few reports have described living foreign bodies in the human body.The current manuscript demonstrates that computed tomography(CT)is an effective tool for accurate preoperative evaluation of living foreign...BACKGROUND Few reports have described living foreign bodies in the human body.The current manuscript demonstrates that computed tomography(CT)is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.The threedimensional(3D)reconstruction technology could clearly display anatomical structures,lesions and adjacent organs,improving diagnostic accuracy and guiding the surgical decision-making process.CASE SUMMARY Herein we describe a 68-year-old man diagnosed with digestive tract perforation and acute peritonitis caused by a foreign body of Monopterus albus.The patient pre-sented to the emergency department with complaints of dull abdominal pain,profuse sweating and a pale complexion during work.A Monopterus albus had entered the patient’s body through the anus two hours ago.During hospitalization,the 3D reconstruction technology revealed a perforation of the middle rectum complicated with acute peritonitis and showed a clear and complete Monopterus albus bone morphology in the abdominal and pelvic cavities,with the Monopterus albus biting the mesentery.Laparoscopic examination detected a large(diameter of about 1.5 cm)perforation in the mid-rectum.It could be seen that a Monopterus albus had completely entered the abdominal cavity and had tightly bitten the mesentery of the small intestine.During the operation,the dead Monopterus albus was taken out.CONCLUSION The current manuscript demonstrates that CT is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.展开更多
The Asian swamp eel （Monopterus albus） is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A bet...The Asian swamp eel （Monopterus albus） is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel＇s immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors （IRFs） play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRFIO have seldom been studied. In this study, we characterized IRF4 and IRFIO from M. albus （malRF4 and malRFlO） and found that malRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid （aa） protein, while malRFlO consists of 1 744 nucleotides including an open reading frame （ORF） of 1 236 nt encoding 411 aa. The malRFlO gene was constitutively expressed at high levels in a variety of tissues, while malRF4 showed a very limited expression pattern. Expression of malRF4 and malRFlO in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic： polycytidylic acid （poly I：C）, lipopolysaccharide （LPS） and a common pathogenic bacteria Aeromonas hydrophila. These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.展开更多
Ghrelin is an important signaling molecule linking reproductive and energy metabolism. In this study, ghrelin gene of Monopterus albus was cloned. Its structure and function were analysized preliminarily. By Rapid Amp...Ghrelin is an important signaling molecule linking reproductive and energy metabolism. In this study, ghrelin gene of Monopterus albus was cloned. Its structure and function were analysized preliminarily. By Rapid Amplification of cDNA Ends(RACE) technique, full-length cDNA and DNA sequences of ghrelin gene were obtained. The full-length ghrelin cDNA(GenBank accession no. JX122807) was 552 bp long, containing a 115 bp 5'-untranslated region, a 324 bp open reading frame and a 113 bp 3'-untranslated region. The full-length ghrelin DNA was 1 323 bp, consisting of three introns and four exons. The exon/intron junction sequences conformed to the GT/AG rule. Three introns were 594, 84 and 93 bp in length, respectively; four exons were229, 78, 112 and 133 bp in length, respectively. The results of amino acid sequence analysis showed that the deduced propreghrelin sequence of M. albus contained a signal peptide(SP) consisting of 22 amino acid residues, a mature peptide(MP)consisting of 19 amino acid residues and a C-terminal amino acid residue. Among them, the third amino acid of MP was serine(Ser^3) as the site for N-acylation and N-deacetylation reactions; the C-terminal amino acid residue sequence might contain a peptide hormone obestatin, which is a physiological antagonist of mature Ghrelin peptide. The homology and phylogenic relationships analyses of amino acid sequences suggested that propreghrelin of M. albus had high similarity to those of several Perciformes fishes; the propreghrelins of M. albus, Perciformes and Heterosomata fishes were clustered into a subgroup. The high conservatism of the gene structure and the amino acid sequences indicated that Ghrelin exerts important physiological functions and plays similar physiological mechanisms in vertebrates.展开更多
The swamp eel(Monopterus albus)is a commonly cultured freshwater fish.Selection and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this species.However,there are currently no guid...The swamp eel(Monopterus albus)is a commonly cultured freshwater fish.Selection and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this species.However,there are currently no guidelines for selecting high-quality males in M.albus.This study quantitatively investigated the sperm motility of male swamp eels during the breeding season by using computer-assisted sperm analysis system.Variability in the proportion and number of motile sperm was observed between individual males.The testes were grouped into high-quality and low-quality according to sperm quality.Morphometric parameters of the two groups were analyzed.We found that high-quality testes were longer(150±27 mm verses 127±19 mm)than low-quality testes.Besides,the proportion of testes with a dark appearance was slightly higher(58.3%verses 48.1%)in the high-quality group than in the low-quality group.Metabolomic analysis was conducted to compare the metabolite profiles of high-quality and low-quality testes.Glycerophospholipids,organic acids,glycerolipids,amino acids,sphingolipids,and nucleotides were the most abundant metabolites in the testes of swamp eels.Moreover,135 differential metabolites were identified.Several nucleotide derivatives,including 50-deoxyadenosine,20-deoxyadenosine,and ribosyl adenosine,were the most significantly enriched metabolites in highquality testes.These metabolites are potential indicators for the testis quality in M.albus.Altogether,our work provides systematic and quantitative data about the physiological and biochemical characteristics of M.albus testis,which provides an evidential basis for the selection of high-fecundity parents to improve artificial reproduction efficiency of M.albus.展开更多
为了研究黄鳝(Monopterus albus)热休克蛋白70(Heat Shock Protein 70,简称HSP70)的结构和功能,试验采用生物信息学方法对黄鳝HSP70的基本理化性质、亲/疏水性、信号肽、磷酸化位点、糖基化、无序化特征、二级结构、三级结构、相互作用...为了研究黄鳝(Monopterus albus)热休克蛋白70(Heat Shock Protein 70,简称HSP70)的结构和功能,试验采用生物信息学方法对黄鳝HSP70的基本理化性质、亲/疏水性、信号肽、磷酸化位点、糖基化、无序化特征、二级结构、三级结构、相互作用蛋白网络及进化树进行了研究。结果表明,黄鳝HSP70由441个氨基酸组成,相对分子质量为48146.31,等电点为5.98,整体呈碱性,是较稳定的亲水性蛋白质,有信号肽,但不存在跨膜结构,属于分泌型蛋白质。黄鳝HSP70存在35个潜在磷酸化位点,其中包括19个Ser、14个Thr和2个Tyr磷酸化位点,有5个典型的N糖基化位点。二级结构以α-螺旋(39.68%)、延伸链(20.41%)、β-折叠(6.58%)和无规则卷曲(33.33%)为主要结构。热休克蛋白70(HSP70)作为分子伴侣,在蛋白质折叠和运输、细胞周期调控、细胞凋亡和精子发生等方面发挥着重要作用。研究表明,黄鳝HSP70在抵抗外界应激源、环境应激保护等方面起着重要作用。展开更多
为深入研究黄鳝Dmrt1基因5′端侧翼序列在性别决定与分化中细胞通路之间的转录调控作用。本研究以黄鳝基因组DNA为模板,PCR扩增出Dmrt1基因5′端侧翼序列,并对该序列进行了生物信息学分析,然后将克隆获得的Dmrt1基因5′端侧翼序列构建到...为深入研究黄鳝Dmrt1基因5′端侧翼序列在性别决定与分化中细胞通路之间的转录调控作用。本研究以黄鳝基因组DNA为模板,PCR扩增出Dmrt1基因5′端侧翼序列,并对该序列进行了生物信息学分析,然后将克隆获得的Dmrt1基因5′端侧翼序列构建到PGL3-enhancer载体中,命名为pGL3-enhancer-Dmrt1,最后将pGL3-enhancer-Dmrt1和内参质粒pRL-TK共转染至HEK293细胞,采用双荧光素酶检测系统对Dmrt1基因5′端侧翼的启动活性进行了检测。实验结果显示:克隆获得的Dmrt1基因5′端侧翼序列为1519 bp;利用TESS-Transcription Element Search System转录因子结合位点在线预测数据库对黄鳝Dmrt1基因5′端侧翼序列进行预测分析结果表明,该序列存在AP-1、Oct-1、C/EBPa和GATAx等真核生物通用的转录因子结合位点以及与性别相关基因的蛋白结合位点,如SRY、Sox3和Sox5等;双荧光素酶检测结果显示,该5′端侧翼序列具有启动活性。展开更多
文摘BACKGROUND Few reports have described living foreign bodies in the human body.The current manuscript demonstrates that computed tomography(CT)is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.The threedimensional(3D)reconstruction technology could clearly display anatomical structures,lesions and adjacent organs,improving diagnostic accuracy and guiding the surgical decision-making process.CASE SUMMARY Herein we describe a 68-year-old man diagnosed with digestive tract perforation and acute peritonitis caused by a foreign body of Monopterus albus.The patient pre-sented to the emergency department with complaints of dull abdominal pain,profuse sweating and a pale complexion during work.A Monopterus albus had entered the patient’s body through the anus two hours ago.During hospitalization,the 3D reconstruction technology revealed a perforation of the middle rectum complicated with acute peritonitis and showed a clear and complete Monopterus albus bone morphology in the abdominal and pelvic cavities,with the Monopterus albus biting the mesentery.Laparoscopic examination detected a large(diameter of about 1.5 cm)perforation in the mid-rectum.It could be seen that a Monopterus albus had completely entered the abdominal cavity and had tightly bitten the mesentery of the small intestine.During the operation,the dead Monopterus albus was taken out.CONCLUSION The current manuscript demonstrates that CT is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.
基金financially supported by the Project from the National Natural Science Foundation of China(31101928)the State Key Laboratory of Freshwater Ecology and Biotechnology(2010FB02)Public Welfare Scientific Research Project of Hubei Province(2012DBA29001)
文摘The Asian swamp eel （Monopterus albus） is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel＇s immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors （IRFs） play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRFIO have seldom been studied. In this study, we characterized IRF4 and IRFIO from M. albus （malRF4 and malRFlO） and found that malRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid （aa） protein, while malRFlO consists of 1 744 nucleotides including an open reading frame （ORF） of 1 236 nt encoding 411 aa. The malRFlO gene was constitutively expressed at high levels in a variety of tissues, while malRF4 showed a very limited expression pattern. Expression of malRF4 and malRFlO in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic： polycytidylic acid （poly I：C）, lipopolysaccharide （LPS） and a common pathogenic bacteria Aeromonas hydrophila. These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.
基金Supported by Natural Science Foundation of Hubei Province(2013CFB393)Open Fund of Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilizations,Ministry of Agriculture(KF201307)+2 种基金National Pillar Program of China(2013BAD20B06)Pillar Program of Hubei Province(2015BBA235)Program for Outstanding Young and Middle-aged Scientific Innovation Team in Colleges and Universities of Hubei Province(T201503)
文摘Ghrelin is an important signaling molecule linking reproductive and energy metabolism. In this study, ghrelin gene of Monopterus albus was cloned. Its structure and function were analysized preliminarily. By Rapid Amplification of cDNA Ends(RACE) technique, full-length cDNA and DNA sequences of ghrelin gene were obtained. The full-length ghrelin cDNA(GenBank accession no. JX122807) was 552 bp long, containing a 115 bp 5'-untranslated region, a 324 bp open reading frame and a 113 bp 3'-untranslated region. The full-length ghrelin DNA was 1 323 bp, consisting of three introns and four exons. The exon/intron junction sequences conformed to the GT/AG rule. Three introns were 594, 84 and 93 bp in length, respectively; four exons were229, 78, 112 and 133 bp in length, respectively. The results of amino acid sequence analysis showed that the deduced propreghrelin sequence of M. albus contained a signal peptide(SP) consisting of 22 amino acid residues, a mature peptide(MP)consisting of 19 amino acid residues and a C-terminal amino acid residue. Among them, the third amino acid of MP was serine(Ser^3) as the site for N-acylation and N-deacetylation reactions; the C-terminal amino acid residue sequence might contain a peptide hormone obestatin, which is a physiological antagonist of mature Ghrelin peptide. The homology and phylogenic relationships analyses of amino acid sequences suggested that propreghrelin of M. albus had high similarity to those of several Perciformes fishes; the propreghrelins of M. albus, Perciformes and Heterosomata fishes were clustered into a subgroup. The high conservatism of the gene structure and the amino acid sequences indicated that Ghrelin exerts important physiological functions and plays similar physiological mechanisms in vertebrates.
基金supported by grants from the National Natural Science Foundation of China(No.31922085)Natural Science Foundation of Hubei Province(Nos.2020CFA056 and 2022BBA0010)Foundation of Hubei Hongshan Laboratory(2022hszd022)to D Luo.
文摘The swamp eel(Monopterus albus)is a commonly cultured freshwater fish.Selection and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this species.However,there are currently no guidelines for selecting high-quality males in M.albus.This study quantitatively investigated the sperm motility of male swamp eels during the breeding season by using computer-assisted sperm analysis system.Variability in the proportion and number of motile sperm was observed between individual males.The testes were grouped into high-quality and low-quality according to sperm quality.Morphometric parameters of the two groups were analyzed.We found that high-quality testes were longer(150±27 mm verses 127±19 mm)than low-quality testes.Besides,the proportion of testes with a dark appearance was slightly higher(58.3%verses 48.1%)in the high-quality group than in the low-quality group.Metabolomic analysis was conducted to compare the metabolite profiles of high-quality and low-quality testes.Glycerophospholipids,organic acids,glycerolipids,amino acids,sphingolipids,and nucleotides were the most abundant metabolites in the testes of swamp eels.Moreover,135 differential metabolites were identified.Several nucleotide derivatives,including 50-deoxyadenosine,20-deoxyadenosine,and ribosyl adenosine,were the most significantly enriched metabolites in highquality testes.These metabolites are potential indicators for the testis quality in M.albus.Altogether,our work provides systematic and quantitative data about the physiological and biochemical characteristics of M.albus testis,which provides an evidential basis for the selection of high-fecundity parents to improve artificial reproduction efficiency of M.albus.
文摘为了研究黄鳝(Monopterus albus)热休克蛋白70(Heat Shock Protein 70,简称HSP70)的结构和功能,试验采用生物信息学方法对黄鳝HSP70的基本理化性质、亲/疏水性、信号肽、磷酸化位点、糖基化、无序化特征、二级结构、三级结构、相互作用蛋白网络及进化树进行了研究。结果表明,黄鳝HSP70由441个氨基酸组成,相对分子质量为48146.31,等电点为5.98,整体呈碱性,是较稳定的亲水性蛋白质,有信号肽,但不存在跨膜结构,属于分泌型蛋白质。黄鳝HSP70存在35个潜在磷酸化位点,其中包括19个Ser、14个Thr和2个Tyr磷酸化位点,有5个典型的N糖基化位点。二级结构以α-螺旋(39.68%)、延伸链(20.41%)、β-折叠(6.58%)和无规则卷曲(33.33%)为主要结构。热休克蛋白70(HSP70)作为分子伴侣,在蛋白质折叠和运输、细胞周期调控、细胞凋亡和精子发生等方面发挥着重要作用。研究表明,黄鳝HSP70在抵抗外界应激源、环境应激保护等方面起着重要作用。
文摘为深入研究黄鳝Dmrt1基因5′端侧翼序列在性别决定与分化中细胞通路之间的转录调控作用。本研究以黄鳝基因组DNA为模板,PCR扩增出Dmrt1基因5′端侧翼序列,并对该序列进行了生物信息学分析,然后将克隆获得的Dmrt1基因5′端侧翼序列构建到PGL3-enhancer载体中,命名为pGL3-enhancer-Dmrt1,最后将pGL3-enhancer-Dmrt1和内参质粒pRL-TK共转染至HEK293细胞,采用双荧光素酶检测系统对Dmrt1基因5′端侧翼的启动活性进行了检测。实验结果显示:克隆获得的Dmrt1基因5′端侧翼序列为1519 bp;利用TESS-Transcription Element Search System转录因子结合位点在线预测数据库对黄鳝Dmrt1基因5′端侧翼序列进行预测分析结果表明,该序列存在AP-1、Oct-1、C/EBPa和GATAx等真核生物通用的转录因子结合位点以及与性别相关基因的蛋白结合位点,如SRY、Sox3和Sox5等;双荧光素酶检测结果显示,该5′端侧翼序列具有启动活性。
