Since the first report on the successful deep cryopreservation of mam-malian embryos in 1972,slow progressing cooling rate has been employ-ed in conventional embryos-freezing techniques;while more recent studieson fre...Since the first report on the successful deep cryopreservation of mam-malian embryos in 1972,slow progressing cooling rate has been employ-ed in conventional embryos-freezing techniques;while more recent studieson freezing preimplantation embryos have focussed on the simplificationof cooling and thawing procedures and improvement of viability of embr-yos.Dimethylsulfoxide (DMSO)has been used as an effective cryoprotec-tant for freezing human and other mammalian embryos.It has been foundthat glycerol and other alcohols are effective to protect embryos fromcryoinjury展开更多
文摘Since the first report on the successful deep cryopreservation of mam-malian embryos in 1972,slow progressing cooling rate has been employ-ed in conventional embryos-freezing techniques;while more recent studieson freezing preimplantation embryos have focussed on the simplificationof cooling and thawing procedures and improvement of viability of embr-yos.Dimethylsulfoxide (DMSO)has been used as an effective cryoprotec-tant for freezing human and other mammalian embryos.It has been foundthat glycerol and other alcohols are effective to protect embryos fromcryoinjury