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小鼠植入前胚胎MuERV-L基因表达特征研究 被引量:1
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作者 邵根宝 高爱民 +3 位作者 徐银学 丁红梅 贾超 刘红林 《南京农业大学学报》 CAS CSCD 北大核心 2007年第4期87-91,共5页
利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组... 利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因(MuERV-L)的表达进行研究,并通过appd icolin(DNA复制抑制剂)、TSA(组蛋白去乙酰化酶特异性抑制剂)分别抑制1-细胞期DNA的复制和合子基因组激活(ZGA)过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。结果表明:MuERV-L基因的转录在受精后9-11 h快速启动,并在2-细胞阶段出现瞬时高表达。抑制试验显示:1-细胞期DNA的复制受抑制后,MuERV-L基因的转录也被显著抑制(P〈0.05);在TSA处理的4-细胞胚胎中,MuERV-L基因显著高表达(P〈0.01),且其水平达到正常2-细胞的转录水平。提示:该基因可能对小鼠胚胎ZGA过程起重要作用。 展开更多
关键词 小鼠 胚胎 鼠内源逆转录病毒样基因(muerv-l) 实时定量RT-PCR 基因表达
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Pivotal role for long noncoding RNAs in zygotic genome activation in mice 被引量:1
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作者 Kang Chen Wenju Liu +6 位作者 Jiang Zhu Xiaochen Kou Yanhong Zhao Hong Wang Cizhong Jiang Shaorong Gao Lan Kang 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第5期958-969,共12页
Vertebrate life begins with fertilization,and then the zygote genome is activated after transient silencing,a process termed zygotic genome activation(ZGA).Despite its fundamental role in totipotency and the initiatio... Vertebrate life begins with fertilization,and then the zygote genome is activated after transient silencing,a process termed zygotic genome activation(ZGA).Despite its fundamental role in totipotency and the initiation of life,the precise mechanism underlying ZGA initiation remains unclear.The existence of minor ZGA implies the possible critical role of noncoding RNAs in the initiation of ZGA.Here,we delineate the expression profile of long noncoding RNAs(lncRNAs)in early mouse embryonic development and elucidate their critical role in minor ZGA.Compared with protein-coding genes(PCGs),lncRNAs exhibit a stronger correlation with minor ZGA.Distinct H3K9me3 profiles can be observed between lncRNA genes and PCGs,and the enrichment of H3K9me3 before ZGA might explain the suspended expression of major ZGA-related PCGs despite possessing PolII pre-configuration.Furthermore,we identified the presence of PolII-enriched MuERV-L around the transcriptional start site of minor ZGA-related lncRNAs,and these repeats are responsible for the activation of minor ZGA-related lncRNAs and subsequent embryo development.Our study suggests that MuERV-L mediates minor ZGA lncRNA activation as a critical driver between epigenetic reprogramming triggered by fertilization and the embryo developmental program,thus providing clues for understanding the regulatory mechanism of totipotency and establishing bona fide totipotent stem cells. 展开更多
关键词 ZGA lncRNA muerv-l
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