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Isolation and Antifungal Evaluation of Rumex cyprius Murb Extracts
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作者 Ahmad Ibrahim Husem Mohammed A. AI-Nuri +2 位作者 Nidal A. Zatar Waheed Jondi Mohammed Saleem Ali-Shtayeh 《Journal of Chemistry and Chemical Engineering》 2012年第6期547-550,共4页
Ethanolic extract of dried Rumex cyprius Murb (polygonaceae) was tested against four pathogenic fungi: three dermatophytes, Microsporum canis, Trichophyton mentagrophytes and T. rubrum, and the causative agent of c... Ethanolic extract of dried Rumex cyprius Murb (polygonaceae) was tested against four pathogenic fungi: three dermatophytes, Microsporum canis, Trichophyton mentagrophytes and T. rubrum, and the causative agent of chalkbrood disease of bees, Ascophaera apis. The extract showed considerable activity against all these fungi. One of the main constituents ofRumex cyprius was isolated and tested for its antimycotic activity. It showed a significant activity against the test fungi and was identified as 1,3,8-trihydroxy-6-methylanthracene-9,10-dione based on its IR, UV-V and 1^H NMR spectra. 展开更多
关键词 1 3 8-trihydroxy-6-methylanthracene-9 10-dione antifungal activity medicinal plants Rumex cyprius murb.
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耻垢分枝杆菌MurB蛋白的可溶性表达、纯化和多克隆抗血清制备
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作者 徐立铭 郑琦 +3 位作者 康健 步云 钱磊 马郁芳 《中国科技论文》 CAS 北大核心 2015年第18期2157-2160,共4页
获得耻垢分枝杆菌MurB纯化蛋白,制备小鼠源性MurB多克隆抗血清。首先,利用PCR技术扩增耻垢分枝杆菌murB基因,连接至pColdII载体并转化至E.coli BL21(DE3)菌株;然后,低温下以IPTG诱导MurB蛋白的表达,并利用Ni-His亲和层析技术纯化MurB蛋... 获得耻垢分枝杆菌MurB纯化蛋白,制备小鼠源性MurB多克隆抗血清。首先,利用PCR技术扩增耻垢分枝杆菌murB基因,连接至pColdII载体并转化至E.coli BL21(DE3)菌株;然后,低温下以IPTG诱导MurB蛋白的表达,并利用Ni-His亲和层析技术纯化MurB蛋白;最后,将MurB纯化蛋白联合免疫佐剂注射BalB/c小鼠,经过初次免疫和2次加强免疫后分离抗血清,并分别利用ELISA和Western blot技术检测制备的抗血清对MurB蛋白的效价和特异性。结果显示:已获得具有理想浓度和纯度的MurB纯化蛋白;已制备具有较高效价的小鼠源性MurB多克隆抗血清,该抗血清能够特异性识别耻垢分枝杆菌可溶性蛋白组分中的MurB蛋白。因此,制备的MurB多克隆抗血清能够应用于分枝杆菌MurB蛋白的检测分析,为MurB功能研究奠定了基础。 展开更多
关键词 微生物学 耻垢分枝杆菌 UDP-N-乙酰葡糖胺烯醇丙酮酸还原酶 murb 多克隆抗血清
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3,5-吡唑烷二酮衍生物与大肠杆菌MurB酶的分子对接研究
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作者 覃初新 李天恩 戴康 《绿色科技》 2017年第20期190-192,共3页
指出了MurB酶是细菌合成细胞壁的重要的合成酶,可以作为抗菌药物的新的靶点。为了开发新的MurB酶的抑制剂,选取了16个3,5-吡唑烷二酮衍生物作为配体,应用计算机辅助药物设计技术,对靶标MurB酶进行了分子对接分析。结果显示:16个化合物和... 指出了MurB酶是细菌合成细胞壁的重要的合成酶,可以作为抗菌药物的新的靶点。为了开发新的MurB酶的抑制剂,选取了16个3,5-吡唑烷二酮衍生物作为配体,应用计算机辅助药物设计技术,对靶标MurB酶进行了分子对接分析。结果显示:16个化合物和MurB酶都有较强的结合,其中12个化合物的对接打分函数超过了对照已知抑制剂;化合物中的3,5-吡唑烷二酮的结构能够和MurB酶形成较强的氢键连接;而且分子中的芳基取代基也能够和MurB酶形成疏水性结合。 展开更多
关键词 肽聚糖 murb 分子对接 3 5-吡唑烷二酮衍生物
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三次NURBS曲线在公路线形设计中的应用研究 被引量:1
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作者 何祎 李金宝 龚德俊 《长沙交通学院学报》 1999年第4期42-46,共5页
提出用三次NURBS曲线建立描述公路线形的统一数学模型,并对用NURBS 曲线描述公路各种线形进行了论证。该模型基于公路三维实体,应用于公路线形设计时,只需掌握公路的起讫点、交角点及曲线半径即可控制线形设计和进行局部... 提出用三次NURBS曲线建立描述公路线形的统一数学模型,并对用NURBS 曲线描述公路各种线形进行了论证。该模型基于公路三维实体,应用于公路线形设计时,只需掌握公路的起讫点、交角点及曲线半径即可控制线形设计和进行局部调整。与传统设计方法和其它数学模型相比,具有高度的统一性和灵活性,为公路CAD 软件开发提供了一条思路。 展开更多
关键词 三次murbS 公路CAD 公路 线路设计 应用
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The influence of airtightness on contaminant spread in MURBs in cold climates 被引量:1
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作者 Philip Mckeen Zaiyi Liao 《Building Simulation》 SCIE EI CSCD 2022年第2期249-264,共16页
Tall buildings in cold climates have unique challenges in maintaining indoor air quality due to stack effect.During the heating season,interior air buoyancy creates large pressure differentials in vertical shafts that... Tall buildings in cold climates have unique challenges in maintaining indoor air quality due to stack effect.During the heating season,interior air buoyancy creates large pressure differentials in vertical shafts that can drive airflow from lower floors into upper floors.This pressure differential can result in the spread of contaminants throughout a building.Most recently,concern over COVID-19 has increased attention to the potential spread of airborne diseases in densely populated buildings.For many multi-unit residential buildings,suite ventilation has traditionally relied upon fresh air supplied through a mechanically pressurized corridor.In cold climates,large pressure differentials created by stack-effect can reduce the effectiveness of this approach.Multizone and CFD simulations are employed to analyze airflow and contaminant spread due to stack effect.Simulations are conducted on an idealized model of a 10-storey building using a range of experimentally derived airtightness parameters.Simulations demonstrate stack effect can reduce corridor ventilation to suites and even reverse the airflow for leakier buildings.Reduced airflow to suites can result in the accumulation of contaminants.Reversal of the airflow can allow contaminants from a suite to spread throughout the building.Contaminant spread is illustrated as a function of mechanical ventilation,building airtightness,and ambient temperatures.Strategies to reduce the influence of stack effect on mechanically pressurized corridors are discussed. 展开更多
关键词 murbs ventilation contamination spread stack effect
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