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DA、Salsolinol及N-methyl-salsolinol的HPLC-ECD检测方法研究 被引量:3
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作者 窦凯飞 杨威 +1 位作者 王冉 邓玉林 《现代科学仪器》 2007年第6期57-59,共3页
采用高效液相色谱结合库仑阵列多电极检测器体系(HPLC-ECD)建立了一套操作简便、高效、快速测定多巴胺(DA)、6,7-二羟基-1-甲基-1,2,3,4-四氢异喹啉(Salsolinol)和6,7-二羟基-1,2-二甲基-1,2,3,4 -四氢异喹啉(N-methyl-salsolinol)的方... 采用高效液相色谱结合库仑阵列多电极检测器体系(HPLC-ECD)建立了一套操作简便、高效、快速测定多巴胺(DA)、6,7-二羟基-1-甲基-1,2,3,4-四氢异喹啉(Salsolinol)和6,7-二羟基-1,2-二甲基-1,2,3,4 -四氢异喹啉(N-methyl-salsolinol)的方法,并研究该检测方法的灵敏性和稳定性,检测物质浓度和响应因子之间的线性关系。DA、Salsolinol及N-methyl-salsolinol的相关系数分别为1、1和0.9997;DA的检测限低于0.1ng/mL,Sal和NMSal的检测限低于0.5ng/mL,各种物质的方法回收率为98.5%-104.7%。 展开更多
关键词 HPLC-ECD SALSOLINOL n-methyl-salsolinol
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N-methyl-D-aspartate receptor subunit 1 regulates neurogenesis in the hippocampal dentate gyrus of schizophrenia-like mice 被引量:4
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作者 Juan Ding Chun Zhang +4 位作者 Yi-Wei Zhang Quan-Rui Ma Yin-Ming Liu Tao Sun Juan Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第12期2112-2117,共6页
N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the bra... N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the brain. Previous studies have paid little attention to the role of the N-methyl-D-aspartate receptor subunit 1 (NR1) in neurogenesis in the hippocampus of schizophrenia. A mouse model of schizophrenia was established by intraperitoneal injection of 0.6 mg/kg MK-801, once a day, for 14 days. In N-methyl-D-aspartate-treated mice, N-methyl-D-aspartate was administered by intracerebroventricular injection in schizophrenia mice on day 15. The number of NR1-, Ki67- or BrdU-immunoreactive cells in the dentate gyrus was measured by immunofluorescence staining. Our data showed the number of NR1-immunoreactive cells increased along with the decreasing numbers of BrdU- and Ki67-immunoreactive cells in the schizophrenia groups compared with the control group. N-methyl-D-aspartate could reverse the above changes. These results indicated that NR1 can regulate neurogenesis in the hippocampal dentate gyrus of schizophrenia mice, supporting NR1 as a promising therapeutic target in the treatment of schizophrenia. This study was approved by the Experimental Animal Ethics Committee of the Ningxia Medical University, China (approval No. 2014-014) on March 6, 2014. 展开更多
关键词 nerve REGENERATION SCHIZOPHRENIA MK-801 n-methyl-D-ASPARTATE NEUROGENESIS n-methyl-D-ASPARTATE receptor n-methyl-Daspartate receptor SUBUNIT 1 BrdU Ki67 HIPPOCAMPAL dentate gyrus HIPPOCAMPAL NEUROGENESIS neural REGENERATION
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Gamma-aminobutyric acid A receptor and N-methyl-D-aspartate receptor subunit expression in rat spiral ganglion neurons 被引量:2
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作者 Xiaolan Tang Meng Gao Shuang Feng Jiping Su 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第13期1020-1024,共5页
BACKGROUND: Gamma-aminobutyric acid A (GABAA) and N-methyl-D-aspartate (NMDA) receptors are significant receptors in the central nervous system. An understanding of GABAA and NMDA receptor expression in spiral ga... BACKGROUND: Gamma-aminobutyric acid A (GABAA) and N-methyl-D-aspartate (NMDA) receptors are significant receptors in the central nervous system. An understanding of GABAA and NMDA receptor expression in spiral ganglion neurons (SGN) provides information for the functional role of these receptors in the auditory system. OBJECTIVE: To investigate mRNA expression of GABAA receptor (GABAAR) and NMDA receptor (NMDAR) subunits in the rat SGN. DESIGN, TIME AND SETTING: This in vitro, molecular biological study was performed at the Laboratory of Otolaryngology-Head and Neck Surgery, Guangxi Medical University, China from July 2007 to May 2008. MATERIALS: Reverse Transcriptase Kit and Taq DNA polymerase were purchased from Fermentas Burlington, ON, Canada; GABAAR and NMDAR primers were purchased from Shanghai Sangon, Shanghai, China. METHODS: SGN from 3-5 day postnatal Wistar rats was collected for primary cultures, mRNA expression of GABAAR and NMDAR subunits in the SGN was determined by reverse transcription polymerase chain reaction. MAIN OUTCOME MEASURES: Expression levels of GABAAR and NMDAR subunits were determined by quantitative analysis. RESULTS: GABAAR subunits (αl 6, β1 3, and y1 3) and NMDAR subunits (NR1, NR2A, NR2B, NR2C, NR2D, NR3A, and NR3B) were detected in the SGN. In α subunit genes of GABAAR, α1 and α3 expression was similar (P 〉 0.05) and greater than the other subunits. Of the β subunit genes, β1 subunit mRNA levels were greater than β2 and β3. Of the y subunit genes, y2 subunit mRNA levels were greater than y1 and y3. NR1 mRNA expression was the greatest of NMDAR subunits. CONCLUSION: GABAAR subunits (α1 6, β1-3, and y1-3) and NMDAR subunits (NR1, NR2A, NR2B, NR2C, NR2D, NR3A, and NR3B) were expressed in the rat SGN. Through comparison of GABAAR and NMDAR subunit expression, possible GABAAR combinations, as well as highly expressed subunit combinations, were estimated, which provided information for pharmacological and electrophysiological characteristics of GABAAR in the auditory system. 展开更多
关键词 spiral ganglion neuron gamma-aminobutyric acid A receptor n-methyl D-aspartate receptor reverse transcription polymerase chain reaction neural regeneration
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Dissolution Properties of 2-(Dinitromethylene)-5-methyl-1,3-diazacyclopentane in Dimethyl Sulfoxide and N-Methyl Pyrrolidone 被引量:2
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作者 XIAO Li-bai XING Xiao-ling +4 位作者 ZHAO Feng-qi XU Kang-zheng YAO Er-gang TAN Yi HAO Hai-xia 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2012年第4期743-746,共4页
The molar enthalpies of dissolution for 2-(dinitromethylene)-5-methyl-1,3-diazacyclopentane(DNMDZ) in dimethyl sulfoxide(DMSO) and N-methyl pyrrolidone(NMP) were measured using an RD496-2000 Calvet microcalori... The molar enthalpies of dissolution for 2-(dinitromethylene)-5-methyl-1,3-diazacyclopentane(DNMDZ) in dimethyl sulfoxide(DMSO) and N-methyl pyrrolidone(NMP) were measured using an RD496-2000 Calvet microcalorimeter at 298.15 K under atmospheric pressure.Empirical formulae for the calculation of the molar enthalpies of dissolution(Δ diss H) were obtained from the experimental data of the dissolution processes of DNMDZ in DMSO or NMP.The relationships between the rate constant(k) and the molality(b) and between the reaction order(n) and the molality(b) were determined.The corresponding kinetic equations describing the two dissolution processes were dα/dt=10^-2.16(1-α) ^1.01 for the dissolution of DNMDZ in DMSO,and dα/dt=10^-2.02(1-α)^ 0.85 for the dissolution of DNMDZ in NMP,respectively. 展开更多
关键词 2-(Dinitromethylene)-5-methyl-1 3-diazacyclopentane DISSOLUTION Kinetics DIMETHYLSULFOXIDE n-methyl pyrrolidone
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Effect of Xingnaojing Injection(醒脑静注射液)on Hippocampal N-methyl-D-aspartic Acid Receptors of Focal Cerebral Ischemia in Rats 被引量:7
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作者 沈思钰 蔡定芳 +3 位作者 陈伟华 刘静 陈虎 应健 《Chinese Journal of Integrated Traditional and Western Medicine》 2003年第1期49-52,共4页
Objective: To observe and elucidate the neuroprotective effect of Xingnaojing (XNJ) injection on hippocampal N-methyl-D-aspartic acid (NMDA) receptors of focal cerebral ischemia in rats. Methods: Cerebral ischemia was... Objective: To observe and elucidate the neuroprotective effect of Xingnaojing (XNJ) injection on hippocampal N-methyl-D-aspartic acid (NMDA) receptors of focal cerebral ischemia in rats. Methods: Cerebral ischemia was established by occluding the middle cerebral artery with an intraluminal suture technique in rats. Neurological deficit score, infarct volume and quantity of NMDA receptors were estimated in all groups and compared. Results: After being treated with XNJ, the score decreased in the initial 6 hours and infarct volume decreased in 24 hours. And within 24 hours, the quantity of NMDA receptors obviously decreased compared with the model group (P<0. 01) It indicated that XNJ could ameliorate neurological behavior of middle cerebral artery occlusion rats and down-regulate the expression of hippocampal NMDA receptors. Conclusion: The neuroprotective effect of XNJ on focal cerebral ischemia is possibly related to down-regulating the expression of NMDA receptors in rats. 展开更多
关键词 Xingnaojing injection focal cerebral ischemia n-methyl-D-aspartic acid receptor NEURO-PROTECTION
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Anti-N-methyl-D-aspartate receptor encephalitis that aggravates after acinetobacter baumannii pneumonia:A case report 被引量:3
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作者 Cheng C Wang Da J Li +1 位作者 Yi Q Xia Kai Liu 《World Journal of Clinical Cases》 SCIE 2017年第9期368-372,共5页
We report an atypical case of anti-N-methyl-D-aspartate receptor encephalitis(ANMDARE). A 27-year-old man diagnosed with ANMDARE received immunotherapy and had a good recovery. However, within one month, he developed ... We report an atypical case of anti-N-methyl-D-aspartate receptor encephalitis(ANMDARE). A 27-year-old man diagnosed with ANMDARE received immunotherapy and had a good recovery. However, within one month, he developed severe status epilepticus and decreased level of conscience with new hyperpyrexia and dyspnea, and was admitted to the emergency intensive care unit. Acinetobacter baumanii were found in the sputum culture; and anti-NMDAR antibodies were positive(titer: 1/80) in the cerebrospinal fluid. Repeated immunotherapy was administered with antibacterial agents, and the patient recovered except for mild psychiatric sequelae. This is the first report of ANMDARE that aggravates after acinetobacter baumannii pneumonia. Awareness and knowledge of this disorder should be extended, especially in the emergency medicine community. 展开更多
关键词 Anti-n-methyl-D-aspartate receptor ENCEPHALITIS ACINETOBACTER BAUMANNII PNEUMONIA Emergency
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Propofol effectively inhibits lithium-pilocarpine-induced status epilepticus in rats via downregulation of N-methyl-D-aspartate receptor 2B subunit expression 被引量:3
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作者 Henglin wang Zhuoqiang Wang +4 位作者 Weidong Mi Cong Zhao Yanqin Liu Yongan Wang Haipeng Sun 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第11期827-832,共6页
Status epilepticus was induced via intraperitoneal injection of lithium-pilocarpine.The inhibitory effects of propofol on status epilepticus in rats were judged based on observation of behavior,electroencephalography ... Status epilepticus was induced via intraperitoneal injection of lithium-pilocarpine.The inhibitory effects of propofol on status epilepticus in rats were judged based on observation of behavior,electroencephalography and 24-hour survival rate.Propofol(12.5-100 mg/kg) improved status epilepticus in a dose-dependent manner,and significantly reduced the number of deaths within 24 hours of lithium-pilocarpine injection.Western blot results showed that,24 hours after induction of status epilepticus,the levels of N-methyl-D-aspartate receptor 2A and 2B subunits were significantly increased in rat cerebral cortex and hippocampus.Propofol at 50 mg/kg significantly suppressed the increase in N-methyl-D-aspartate receptor 2B subunit levels,but not the increase in N-methyl-D-aspartate receptor 2A subunit levels.The results suggest that propofol can effectively inhibit status epilepticus induced by lithium-pilocarpine.This effect may be associated with downregulation of N-methyl-D-aspartate receptor 2B subunit expression after seizures. 展开更多
关键词 PROPOFOL status epilepticus n-methyl-D-aspartate receptor 2A 2B subunit cerebral cortex HIPPOCAMPUS ELECTROENCEPHALOGRAM
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Heat of absorption of CO_2 in aqueous N,N-diethylethanolamine+ N-methyl-1,3-propanediamine solutions at 313 K 被引量:2
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作者 Katarzyna Sobala Hanna Kierzkowska-Pawlak 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2019年第3期628-633,共6页
Differential heat of absorption of CO_2 in aqueous solutions of N,N-diethylethanolamine(DEEA) and activated DEEA solutions up to a total concentration of 2 mol·L^(-1) was measured as a function of CO_2 loading at... Differential heat of absorption of CO_2 in aqueous solutions of N,N-diethylethanolamine(DEEA) and activated DEEA solutions up to a total concentration of 2 mol·L^(-1) was measured as a function of CO_2 loading at 313.15 K using a reaction calorimeter. In order to analyze the performance of N-methyl-1,3-propanediamine(MAPA)as an activator, DEEA blended solutions containing 0.05, 0.1 and 0.2 mol·L^(-1) MAPA were studied. The heat of CO_2 absorption in single DEEA solutions was unaffected by changing the DEEA concentration in the range of(0.5–2) mol·L^(-1). On the other hand, increasing the concentration of MAPA in aqueous amine mixtures of(DEEA + MAPA) raised the heat of absorption. 展开更多
关键词 CO2 CAPTURE Differential heat of absorption N N-diethylethanolamine n-methyl-1 3-propanediamine AMINE blends
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Influences of levodopa on expression of N-methyl-D-aspartate receptor-1-subunit in the visual cortex of monocular deprivation rats 被引量:2
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作者 Xiao-Nan Sun Jin-Song Zhang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2012年第1期50-54,共5页
AIM: Many studies have demonstrated N-methyl-D-aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also... AIM: Many studies have demonstrated N-methyl-D-aspartate receptor-1-subunit (NMDAR1) is associated with amblyopia. The effectiveness of levodopa in improving the visual function of the children with amblyopia has also been proved. But the mechanism is undefined. Our study was to explore the possible mechanism. METHODS: Sixty 14-day-old healthy SD rats were randomly divided into 4 groups, including normal group, monocular deprivation group, levodopa group and normal saline group, 15 rats each. We sutured all the rats' unilateral eyelids except normal group to establish the monocular deprivation animal model and raise them in normal sunlight till 45-day-old. NMDAR1 was detected in the visual cortex with immunohistochemistry methods, Western Blot and Real time PCR. LD and NS groups were gavaged with levodopa (40mg/kg) and normal saline for 28 days respectively. NMDAR1 was also detected with the methods above. RESULTS: NMDAR1 in the visual cortex of MD group was less than that of normal group. NMDAR1 in the visual cortex of LD group was more than that of NS group. CONCLUSION: NMDAR1 is associated with the plasticity of visual development. Levodopa may influence the expression of NMDAR1 and improve visual function, and its target may lie in the visual cortex. 展开更多
关键词 LEVODOPA n-methyl-D-aspartate receptor monocular deprivation
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Protective effects of naringenin eye drops on N-methylN-nitrosourea-induced photoreceptor cell death in rats 被引量:1
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作者 Jun-Li Lin Yan-Dong Wang +4 位作者 Yan Ma Chun-Mei Zhong Mei-Rong Zhu Wen-Pei Chen Bao-Qin Lin 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2014年第3期391-396,共6页
AIM:To investigate the effects of naringenin eye drops on N-methyl-N-nitrosourea (MNU)-induced photoreceptor cell death in rats.METHODS:Photoreceptor cell death was induced by single intraperitoneal injection of MNU(6... AIM:To investigate the effects of naringenin eye drops on N-methyl-N-nitrosourea (MNU)-induced photoreceptor cell death in rats.METHODS:Photoreceptor cell death was induced by single intraperitoneal injection of MNU(60 mg/kg)in rats.Both eyes of all animals were instilled with one drop of vehicle,0.5% or 1.0% naringenin eye drops three times per day from 7d before to 17d after MNU injection.Effects of naringenin on MNU-induced photoreceptor cell death were evaluated by electrophysiological and histological analysis.RESULTS:Flash electroretinography (FERG)and oscillatory potentials (OPs) recordings showed that the vehicle control group had remarkable reduction of amplitudes and prolongation of latency times.FERG and OPs responses were significantly reversed in MNUinduced rats treated with 0.5%or 1.0% naringenin eye drops compared with the vehicle control.The retinal morphological results showed that naringenin dosedependently preserved the outer nuclear layer,outer retina and total retina.CONCLUSION:These results indicate that topical treatment with naringenin eye drops prevented retinal neurons from MNU-induced structural and functional damages. 展开更多
关键词 NARINGENIN n-methyl-N-NITROSOUREA photoreceptor cell death retinitis pigmentosa
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Synthesis and Structure Analysis of a Tripeptide Containing N-methyl Group Amino Acid 被引量:2
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作者 甄小丽 马振杰 +3 位作者 田霞 李营 韩建荣 刘守信 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2016年第5期718-724,共7页
A convenient method of synthesizing a tripeptide-containing N-methyl group amino acid was developed using O-benzotriazole-N,N,N',N'-tetramethyluronium-hexafluorophosphate as the condensing agent. The crystals of tri... A convenient method of synthesizing a tripeptide-containing N-methyl group amino acid was developed using O-benzotriazole-N,N,N',N'-tetramethyluronium-hexafluorophosphate as the condensing agent. The crystals of tripeptide had white needles belonging to the orthorhombic space group P2_12_12_1. The conformational preference for homochiral tripeptides with one N-methylated amide bond was also investigated. Crystal-structure analysis showed that homochiral tripeptides with an internal N-methylated amide bond preferred a trans-amide form, thereby giving the peptide β-fold characteristics. Intermolecular C-H···O and N-H···O hydrogen bonds linked the molecules into a one-dimensional chain and stabilized the structure. 展开更多
关键词 tripeptide n-methyl amino acid synthesis crystal structure hydrogen bond
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Water-soluble lipopolymer delivery of N-methyl-D-aspartic acid receptor 2B siRNA relieves chronic neuropathic pain in rats 被引量:1
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作者 Jianhua Lu Yuanxiang Tao +4 位作者 Xue Yang Weifeng Tu Hao Chen Jiaxiang Xiong Chungui Hu 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第29期2279-2283,共5页
Spinal dorsal horn N-Methyl-D-aspartic acid receptor 2B (NR2B) overexpression plays an important role in the production and maintenance of neuropathic pain. Because small interfering RNA (siRNA) can inhibit NR2B e... Spinal dorsal horn N-Methyl-D-aspartic acid receptor 2B (NR2B) overexpression plays an important role in the production and maintenance of neuropathic pain. Because small interfering RNA (siRNA) can inhibit NR2B expression, siRNA may provide a novel approach to treat neuropathic pain and possibly nerve injury. However, an efficient and safe vector for NR2B siRNA has not been discovered. This study shows that a water soluble lipopolymer (WSLP) comprised of low molecular weight polyethyleneimine (PEI) and cholesterol can deliver siRNA targeting NR2B for the treatment of neuropathic pain. Results show that intrathecal injection of WSLP/siRNA complexes for 3 days inhibit NR2B gene expression with reductions in mRNA and protein levels by 59% and 54%, respectively, compared with control rats (P 〈 0.01). Injection of WSLP complexed with scrambled siRNA, or PEI with siRNA did not show this inhibitory effect. Moreover, injection of WSLP/siRNA complexes significantly relieved neuropathic pain at 3, 7, 12, and 21 days, while injection of WSLP with scrambled siRNA or PEI with siRNA did not. These results demonstrate that WSLP can efficiently deliver siRNA targeting NR2B in vivo and relieve neuropathic pain. 展开更多
关键词 water soluble lipopolymer n-methyl-D-aspartic acid receptor 2B small interfering RNA peripheral nerve injury neuropathic pain
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STUDIES ON THE INITIATION MECHANISM OF ORGANIC PEROXIDE AND N-METHACRYLOYLOXYETHYL-N-METHYL ANILINE IN METHYL METHACRYLATE POLYMERIZATION 被引量:1
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作者 丘坤元 郭大捷 +1 位作者 郭新秋 冯新德 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 1990年第4期363-370,共8页
The initiation mechanism of methyl methacrylate (MMA) polymerization by organic peroxide and polymerizable aromatic tertiary amine such as N-methacryloyloxyethyl-N-methyl aniline (MEMA) binary system has been studied.... The initiation mechanism of methyl methacrylate (MMA) polymerization by organic peroxide and polymerizable aromatic tertiary amine such as N-methacryloyloxyethyl-N-methyl aniline (MEMA) binary system has been studied. The kinetics of polymerization of MMA and the ESR spectra of organic peroxide/MEMA system were determined. Based on the ESR study and the end-group analysis by UV spectra of the polymer formed, the initiation mechanism is proposed. 展开更多
关键词 Organic peroxide/N-methacryloyloxyethyl-n-methyl aniline initiation system Redox initiator MMA polymerization ESR End group analysis.
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Anesthetic Management of Patients with Anti-N-methyl-D-aspartate Receptor Encephalitis:A Report of Two Cases
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作者 Xiaoling Zhang Jian Li Dongxin Wang 《Chinese Medical Sciences Journal》 CAS CSCD 2017年第1期62-64,共3页
ANTI-N-METHYL-D-ASPARTATE (NMDA) receptorencephalitis is a newly recognized autoimmunedisease. It is predominantly described in youngwomen with a series of symptoms, includingpersonality change, memory loss, seizures,... ANTI-N-METHYL-D-ASPARTATE (NMDA) receptorencephalitis is a newly recognized autoimmunedisease. It is predominantly described in youngwomen with a series of symptoms, includingpersonality change, memory loss, seizures, involuntarymovements, autonomic dysfunction et al.1 It is commonlyassociated with mature ovarian teratomas.2 Since its firstdenomination by Dalmau et al,1 many scientific publicationshave emerged on anti-NMDA receptor encephalitis, butonly a few focused on the anesthetic management ofpatients with this disease.3-5 Herein we reported two caseswith anti-NMDA receptor encephalitis in association withovarian teratoma and discussed the anesthetic managementand the outcomes of these patients. 展开更多
关键词 anti-n-methyl-D-aspartate receptor ENCEPHALITIS ANESTHETIC management OVARIAN TERATOMA
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Changes in synaptic and extrasynaptic N-methyl-D-aspartate receptor-mediated currents at early-stage epileptogenesis in adult mice
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作者 Juegang Ju Sheng-tian Li 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第2期118-124,共7页
Previous reports have shown that N-methyl-D-aspartate (NMDA) receptors are extensively involved in epilepsy genesis and recurrence. Recent studies have shown that synaptic and extrasynaptic NMDA receptors play diffe... Previous reports have shown that N-methyl-D-aspartate (NMDA) receptors are extensively involved in epilepsy genesis and recurrence. Recent studies have shown that synaptic and extrasynaptic NMDA receptors play different, or even opposing, roles in various signaling pathways, including synaptic plasticity and neuronal death. The present study analyzed changes in synaptic and extrasynaptic NMDA receptor-mediated currents during epilepsy onset. Mouse models of lithium chloride pilocarpLne-induced epilepsy were established, and hippocampal slices were prepared at 24 hours after the onset of status epilepticus. Synaptic and extrasynaptic NMDA receptor-mediated excitatory post-synaptic currents (NMDA-EPSCs) were recorded in CA1 pyramidal neurons by whole-cell patch clamp technique. Results demonstrated no significant difference in rise and delay time of synaptic NMDA-EPSCs compared with normal neurons. Peak amplitude, area-to-peak ratio, and rising time of extrasynaptic NMDA-EPSCs remained unchanged, but decay of extrasynaptic NMDA-EPSCs was faster than that of normal neurons, These results suggest that extrasynaptic NMDA receptors play a role in epileptogenesis. 展开更多
关键词 n-methyl-D-aspartate receptor excitatory postsynaptic current epilepsy EPILEPTOGENESIS hippocampus
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Dissolution and recrystallization of perovskite induced by N-methyl-2-pyrrolidone in a closed steam annealing method
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作者 Lili Zhi Yanqing Li +4 位作者 Xiaobing Cao Yahui Li Xian Cui Lijie Ci Jinquan Wei 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2019年第3期78-83,共6页
High quality perovskite films with large columnar grains are greatly desired for efficient perovskite solar cells. Here, low volatility N-methyl-2-pyrrolidone(NMP) was added in MAI/IPA solution in a two-step spin-coat... High quality perovskite films with large columnar grains are greatly desired for efficient perovskite solar cells. Here, low volatility N-methyl-2-pyrrolidone(NMP) was added in MAI/IPA solution in a two-step spin-coating method, which promoted the conversion of lead iodide to perovskite. The perovskite films were annealed by a closed-steam annealing method to prolong the recrystallization process of perovskite films assisted by the residual NMP. It leaded to high quality CH_3NH_3PbI_3 perovskite films with large columnar grains due to its enhancement of the Oswald ripening. The large grain perovskite film leaded to efficient carrier transformation and injection, and low recombination. The photovoltaic performance of the perovskite solar cells was improved significantly. 展开更多
关键词 PEROVSKITE solar cell n-methyl-2-PYRROLIDONE (NMP) DISSOLUTION and RECRYSTALLIZATION Closed-steam ANNEALING Oswald RIPENING
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Effect of N-methyl-aspartate and Betaine on Growth Perfor-mance and Correlation Between Growth Hormone,Growth Performance and Carcass Composition in Finishing Pigs
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作者 XU Zi-rong, FENG Jie and ZOU Xiao-ting( Key Laboratory for Molecular Animal Nutrition of Ministry of Education , Hangzhou 310029) 《Agricultural Sciences in China》 CAS CSCD 2002年第3期333-337,共5页
Ninety finishing pigs were selected to study the effect of N-methyl-aspartate and betaine on the internal growth hormone level in the serum and the correlation between the growth hormone level, growth performance and ... Ninety finishing pigs were selected to study the effect of N-methyl-aspartate and betaine on the internal growth hormone level in the serum and the correlation between the growth hormone level, growth performance and carcass characteristic of finishing pig. The study showed that the two matters could improve pig growth and carcass composition significantly. The correlation analyses indicated that the growth hormone and IGF-I have a positive correlation with the growth rate. Carcass lean ratio, longissimus dorsi area, serum free fatty acid and lipase activity have a negative correlation with the feed conversion ratio, carcass fat ratio and urine nitrogen. But the growth hormone is more effective than IGF-I (P<0.01). The results implicated that both the two matters may act through growth hormone axis (growth hormone - IGF-I) to manipulate pig growth. 展开更多
关键词 n-methyl-aspartate BETAINE Growth hormone Growth performance Carcass composition Pig
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Visible Light-induced Photoluminescence of a Zinc(Ⅱ) Complex of N-methyl-4,4-bipyridinium Cation
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作者 DU Ming-Xiu YANG Chen +1 位作者 CAI Li-Zhen GUO Guo-Cong 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2019年第2期317-324,169,共9页
Compounds with visible-light-induced photoluminescence are particularly attractive in acting as the emitting components of light-emitting diodes and fluorescent probes for biological applications. A new complex [Zn(NC... Compounds with visible-light-induced photoluminescence are particularly attractive in acting as the emitting components of light-emitting diodes and fluorescent probes for biological applications. A new complex [Zn(NCS)_3(MQ)](MQ^+ = N-methyl-4,4?-bipyridinium) with an isolated molecular structure has been hydrothermally synthesized and characterized by single-crystal X-ray diffraction, elemental analysis, IR spectroscopy and solid-state photoluminescent determination. Crystal data: C_(14)H_(11)N_5S_3Zn, M_r = 410.83, monoclinic, space group P2_1/c, a = 8.809(5), b = 13.138(7), c = 15.580(9) ?, b = 104.433(10)°, V = 1746.2(17) ?~3, Z = 4, D–c = 1.563 g×cm^3, μ = 1.769 mm^(–1), F(000) = 832, the final R = 0.0376 and wR = 0.1243 for 2850 observed reflections with I > 2s(I). In the crystal structure, the zinc atom is tetrahedrally coordinated by one MQ+ ligand and three NCS– groups to yield a tetrahedral configuration. The bond length of Zn–NMQ is clearly shorter than those found in reported Zn(Ⅱ) complexes of 4,4?-bipy. The isolated units are joined by C–H···S interactions to generate a 2D hydrogen-bonding network. This compound displays visible light-induced photoluminescence, which originates from both intraligand charge-transfer of the MQ+ ligand and NCS~–→MQ^+ charge-transfer according to a density of states(DOS) calculation. Moreover, its emission band shows a clear blue shift compared with those of halide compounds. 展开更多
关键词 ZINC COMPLEX n-methyl-4 4-bipyridinium PHOTOLUMINESCENCE visible-light excitation
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Identification of N-Methyl Bis(2-(Alkyloxy-Alkylphosphoryloxy)Ethyl) Amines by LC-HRMS/MS
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作者 Huilan Yu Shilei Liu +2 位作者 Daoming Sun Chengxin Pei Yu Xiang 《American Journal of Analytical Chemistry》 2014年第13期820-827,共8页
N-Methyl bis(2-(alkyloxy-alkylphosphoryloxy)ethyl)amines, which are abbreviated as PNPs, are a series of new skeleton chemicals belonging to schedule 2.B.04 chemicals of Chemical Weapons Convention (CWC). PNPs are imp... N-Methyl bis(2-(alkyloxy-alkylphosphoryloxy)ethyl)amines, which are abbreviated as PNPs, are a series of new skeleton chemicals belonging to schedule 2.B.04 chemicals of Chemical Weapons Convention (CWC). PNPs are important markers of chemical warfare agents because they are structurally relative to both nerve agents and N-mustards. In this study, fragmentation pathways of the most characteristic fragment ions in Q-TOF mass spectrometry were proposed based on the information from accurate mass and secondary fragmentations of product ions scan experiments. Results indicated that the base ion in LC/HRMS was the quasi-molecular ion [M+H]+. In LC-HRMS/MS, it was [M+H-CnH2n+1P(O)(OH)CmH2m+1O]+ fragment ion which was formed by losing an alkyloxy alkylphosphoryloxy group from the quasi-molecular ion. The diagnostic ion m/z84.0814 was identified as [C5H10N]+, which was the group of (CH2=CH)2N+(H)CH3. PNPs have two protonated centers. One is on the N atom, the other is on the O atom (P=O). O-n-propyl PNPs generally exhibited two fragmentation pathways. Firstly, the quasi-molecular ion [M+H]+ lost a propoxy alkylphosphoryloxy group to produce [R1P(OH+)(O-n-C3H7)OCH2CH2N(CH3)CH=CH2]+, which could be fragmented further to produce [C5H10N]+ ion. Secondly, [R1P(OH+)(O-n-C3H7) OCH=CH2]+ ions were produced from [M+H]+ and fragmented further to produce the abundant ions [R1P(OH+)(OH)OCH =CH2]. However, O-isopropyl PNPs characteristically produced weak fragment ions [M+H-C3H6]+, which were presumably formed via loss of CH3CH=CH2 from [M+H]+. Other PNPs showed similar fragmentation pathways as O-n-propyl PNPs. On the summarization of the MS fragmentation pathways of PNPs, LC-HRMS/MS quantitative and qualitative methods were developed and applied to analyze N-Methyl bis(2-(butoxy-methylphosphoryloxy)ethyl]amine in high background organic samples. The analytical results had successfully supported the sample preparation for the 33rd official proficiency test of Organization for Prohibition of Chemical Weapons (OPCW). 展开更多
关键词 Chemical Weapons LC-HRMS/MS n-methyl Bis(2-(Alkyloxy-Alkylphosphoryloxy)Ethyl)Amines Fragmentation Pathways Analysis
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Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters
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作者 Yaoyu Li An'an Yang +3 位作者 Tingting Zhu Zhao Liu Siwei You Kwok-Fai So 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第10期725-730,共6页
This study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal gangl... This study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect. 展开更多
关键词 retinal ganglion cells optic nerve transection n-methyl-D-aspartate receptor nitric oxide synthase neuronal survival HAMSTER
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