A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China. The objective of the present study was to evaluate the cross-protective e...A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China. The objective of the present study was to evaluate the cross-protective efficacy of the live vaccine TJM-F92 in young pigs against challenge with a NADC30-like strain, HN201605. Twenty-five PRRSV-and antibody-free pigs were randomly divided into the following five groups: Vac/ChA, Unvac/ChA, Vac/ChB, Unvac/ChB and the mock.The pigs in groups Vac/ChA and Vac/ChB were inoculated intramuscularly with 1 mL TJM-F92(10^(5.0) TCID_(50)/mL). At28 days post vaccination(0 days post challenge), groups Vac/ChA and Unvac/ChA were inoculated intranasally with 10^(4.5) TCID_(50)/mL PRRSV strain TJ F3(2 mL/pig), while groups Vac/ChB and Unvac/Ch B were inoculated, using the same route, with the same dose of the NADC30-like strain HN201605 F3. Protective effects of the PRRSV strain were observed in all pigs in the Vac/ChA and Vac/ChB groups. Neither high fever nor signs of clinical disease were observed through the experiment in these groups, whereas pigs in Unvac/ChA group exhibited serious clinical symptoms, pathological lesions,and weight loss. In Unvac/ChB group, pigs developed milder clinical symptoms, which demonstrated that the NADC30-like strain HN201605 had moderate pathogenicity. The results suggest that the MLV vaccine strain TJM-F92 is an effective and safe vaccine candidate for use in China.展开更多
猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS),俗称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)引的一种猪繁殖和呼吸系统障碍的传染病。我国蓝耳...猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS),俗称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)引的一种猪繁殖和呼吸系统障碍的传染病。我国蓝耳病主要流行虽仍以经典毒株、高致病性毒株为主,但NADC30-like、NADC34-like、QYYZ-like等毒株的流行呈现逐渐上升趋势。目前我国仍未针对NADC30-like(及NADC34-like)毒株推出具有免疫保护的商品化疫苗,且相关基础研究的报道仍较少,造成NADC30-like的田间流行较难防控;现就类NADC30毒株在全球范围内关于致病性、商品疫苗保护力、疫苗研发进展等方面的研究进展进行整理,为猪场防治类NADC30毒株提供基础数据和防控思路。展开更多
猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)是目前严重威胁养猪业发展的重要疫病之一,主要引起母猪繁殖障碍和仔猪呼吸道疾病。1987年美国首次报道该病以来,其毒株不断发生演变。2012年我国出现新的类NAD...猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)是目前严重威胁养猪业发展的重要疫病之一,主要引起母猪繁殖障碍和仔猪呼吸道疾病。1987年美国首次报道该病以来,其毒株不断发生演变。2012年我国出现新的类NADC30毒株(NADC30-like毒株),该毒株可导致母猪流产及仔猪死淘率升高,给养猪业带来新的威胁,因此了解NADC30-like毒株在我国的流行情况及防控措施,对保障我国生猪健康养殖具有重要意义。展开更多
通过临床症状观察、病理学检测、RT-PCR检测与序列分析等方法,对疑似患猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)的病猪进行诊断。送检病猪临床表现发热、呼吸困难、皮肤发绀;剖检病变为肺脏实变、淋巴...通过临床症状观察、病理学检测、RT-PCR检测与序列分析等方法,对疑似患猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)的病猪进行诊断。送检病猪临床表现发热、呼吸困难、皮肤发绀;剖检病变为肺脏实变、淋巴结肿大出血;组织学变化主要包括间质性肺炎、淋巴结出血及多核巨细胞浸润;免疫组化结果显示,猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)抗原主要定位于肺泡上皮细胞和巨噬细胞;病料RT-PCR结果呈PRRSV(命名为SJZ201701株)阳性;序列分析结果表明,该病毒的核衣壳(Nucleocapsid,N)蛋白基因与NADC30株之间基因核苷酸、氨基酸的同源性最高,分别为96.3%和84.4%。遗传进化分析表明,SJZ201701株与NADC30参考毒株关系最近。综合判定该病例为类NADC30株PRRSV引起的感染。展开更多
文摘A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China. The objective of the present study was to evaluate the cross-protective efficacy of the live vaccine TJM-F92 in young pigs against challenge with a NADC30-like strain, HN201605. Twenty-five PRRSV-and antibody-free pigs were randomly divided into the following five groups: Vac/ChA, Unvac/ChA, Vac/ChB, Unvac/ChB and the mock.The pigs in groups Vac/ChA and Vac/ChB were inoculated intramuscularly with 1 mL TJM-F92(10^(5.0) TCID_(50)/mL). At28 days post vaccination(0 days post challenge), groups Vac/ChA and Unvac/ChA were inoculated intranasally with 10^(4.5) TCID_(50)/mL PRRSV strain TJ F3(2 mL/pig), while groups Vac/ChB and Unvac/Ch B were inoculated, using the same route, with the same dose of the NADC30-like strain HN201605 F3. Protective effects of the PRRSV strain were observed in all pigs in the Vac/ChA and Vac/ChB groups. Neither high fever nor signs of clinical disease were observed through the experiment in these groups, whereas pigs in Unvac/ChA group exhibited serious clinical symptoms, pathological lesions,and weight loss. In Unvac/ChB group, pigs developed milder clinical symptoms, which demonstrated that the NADC30-like strain HN201605 had moderate pathogenicity. The results suggest that the MLV vaccine strain TJM-F92 is an effective and safe vaccine candidate for use in China.
文摘猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS),俗称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)引的一种猪繁殖和呼吸系统障碍的传染病。我国蓝耳病主要流行虽仍以经典毒株、高致病性毒株为主,但NADC30-like、NADC34-like、QYYZ-like等毒株的流行呈现逐渐上升趋势。目前我国仍未针对NADC30-like(及NADC34-like)毒株推出具有免疫保护的商品化疫苗,且相关基础研究的报道仍较少,造成NADC30-like的田间流行较难防控;现就类NADC30毒株在全球范围内关于致病性、商品疫苗保护力、疫苗研发进展等方面的研究进展进行整理,为猪场防治类NADC30毒株提供基础数据和防控思路。
文摘猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)是目前严重威胁养猪业发展的重要疫病之一,主要引起母猪繁殖障碍和仔猪呼吸道疾病。1987年美国首次报道该病以来,其毒株不断发生演变。2012年我国出现新的类NADC30毒株(NADC30-like毒株),该毒株可导致母猪流产及仔猪死淘率升高,给养猪业带来新的威胁,因此了解NADC30-like毒株在我国的流行情况及防控措施,对保障我国生猪健康养殖具有重要意义。
文摘通过临床症状观察、病理学检测、RT-PCR检测与序列分析等方法,对疑似患猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)的病猪进行诊断。送检病猪临床表现发热、呼吸困难、皮肤发绀;剖检病变为肺脏实变、淋巴结肿大出血;组织学变化主要包括间质性肺炎、淋巴结出血及多核巨细胞浸润;免疫组化结果显示,猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)抗原主要定位于肺泡上皮细胞和巨噬细胞;病料RT-PCR结果呈PRRSV(命名为SJZ201701株)阳性;序列分析结果表明,该病毒的核衣壳(Nucleocapsid,N)蛋白基因与NADC30株之间基因核苷酸、氨基酸的同源性最高,分别为96.3%和84.4%。遗传进化分析表明,SJZ201701株与NADC30参考毒株关系最近。综合判定该病例为类NADC30株PRRSV引起的感染。
