Objective Alkaline comet assay was used to evaluate DNA repair (nucleotide excision repair, NER) capacity of human fresh lymphocytes from 12 young healthy non-smokers (6 males and 6 females). Methods Lymphocytes were ...Objective Alkaline comet assay was used to evaluate DNA repair (nucleotide excision repair, NER) capacity of human fresh lymphocytes from 12 young healthy non-smokers (6 males and 6 females). Methods Lymphocytes were exposed to UV-C (254 nm) at the dose rate of 1.5 J/m2/sec. Novobiocin (NOV) and aphidicolin (APC), DNA repair inhibitors, were utilized to imitate the deficiency of DNA repair capacity at the incision and ligation steps of NER. Lymphocytes from each donor were divided into three grougs: UVC group, UVC plus NOV group, and UVC plus APC group. DNA single strand breaks were detected in UVC irradiated cells incubated for 0, 30, 60, 90, 120, 180, and 240 min after UVC irradiation. DNA repair rate (DRR) served as an indicator of DNA repair capacity. Results The results indicated that the maximum DNA damage (i.e. maximum tail length) in the UVC group mainly appeared at 90 min. The ranges of DRRs in the UVC group were 62.84%-98.71%. Average DRR value was 81.84%. The DRR difference between males and females was not significant (P<0.05). However, the average DRR value in the UVC plus NOV group and the UVC plus APC group was 52.98% and 39.57% respectively, which were significantly lower than that in the UVC group (P<0.01). Conclusion The comet assay is a rapid, simple and sensitive screening test to assess individual DNA repair (NER) capacity. It is suggested that the time to detect DNA single strand breaks in comet assay should include 0 (before UV irradiation), 90 and 240 min after exposure to 1.5 J·m-2 UVC at least. The DRR, as an indicator, can represent the individual DNA repair capacity in comet assay.展开更多
随着“智能油田”的建设加快,构建基于海量石油数据的智能分析系统意义重大。然而,由于石油生产过程中产生的文本数据往往无结构且类型多样,从中抽取关键信息进行分析成为一个研究热点,而信息抽取又需要高质量的语义实体做支撑。根据这...随着“智能油田”的建设加快,构建基于海量石油数据的智能分析系统意义重大。然而,由于石油生产过程中产生的文本数据往往无结构且类型多样,从中抽取关键信息进行分析成为一个研究热点,而信息抽取又需要高质量的语义实体做支撑。根据这一特定问题,提出基于命名实体识别(Named Entity Recognition,NER)技术针对石油非结构化文本进行信息抽取,构建双向长短时记忆(Bidirectional Long Short-Term Memory,Bi LSTM)网络模型提取语料特征,并结合条件随机场(Conditional Random Field,CRF)做分类器,构建了基于Bi LSTM+CRF的高精度NER模型,针对石油工业领域的非结构化文本进行命名实体抽取。通过在修井作业文本数据集上进行对比实验表明,本方法具有较高的精确率和召回率。展开更多
We investigated bending moment resistance under diagonal compression load of corner doweled joints with plywood members. Joint members were made of 11-ply hardwood plywood of 19 mm thickness. Dowels were fabricated of...We investigated bending moment resistance under diagonal compression load of corner doweled joints with plywood members. Joint members were made of 11-ply hardwood plywood of 19 mm thickness. Dowels were fabricated of Beech and Hornbeam species. Their diameters(6, 8 and 10 mm) and depths of penetration(9, 13 and 17 mm) in joint members were chosen variables in our experiment. By increasing the connector's diameter from 6 to 8 mm, the bending moment resistance under diagonal compressive load was increased, while it decreased when the diameter was increased from 8 to 10 mm. The bending moment resistance under diagonal compressive load was increased by increasing the dowel's depth of penetration. Joints made with dowels of Beech had higher resistance than dowels of Hornbeam. Highest resisting moment(45.18 N·m) was recorded for joints assembled with 8 mm Beech dowels penetrating 17 mm into joint members Lowest resisting moment(13.35 N·m) was recorded for joints assembled with 6 mm Hornbeam dowels and penetrating 9 mm into joint members.展开更多
Carbon nanotubes can carry protein into cells to induce biological effects. Amino-functionalized carbon nanotubes are soluble and biocompatible, have high reactivity and low toxicity, and can help promote nerve cell g...Carbon nanotubes can carry protein into cells to induce biological effects. Amino-functionalized carbon nanotubes are soluble and biocompatible, have high reactivity and low toxicity, and can help promote nerve cell growth. In this study, amino-functionalized ethylenediamine-treated multi-walled carbon nanotubes were used to prepare carbon nanotubes-nerve growth factor complexes by non-covalent grafting. The physicochemical properties, cytotoxicity to PC12 and chick embryo dorsal root ganglion, and biological activity of the carbon nanotubes-nerve growth factor complexes were investigated. The results showed that amino functionalization improved carbon nanotubes-nerve growth factor complex dispersibility, reduced their toxicity to PC12 cells, and promoted PC12 cell differentiation and chick embryo dorsal root ganglion.展开更多
基金Supported by the Key Breeding Project of Sichuan Province during National Eleventh-five Year Plan (2006YZGG25 )Key Quality Project of Sichuan Province (2006YZGG223)Foundation for Young Scientists of Sichuan Provincial Financial Breeding Project in 2008~~
文摘Objective Alkaline comet assay was used to evaluate DNA repair (nucleotide excision repair, NER) capacity of human fresh lymphocytes from 12 young healthy non-smokers (6 males and 6 females). Methods Lymphocytes were exposed to UV-C (254 nm) at the dose rate of 1.5 J/m2/sec. Novobiocin (NOV) and aphidicolin (APC), DNA repair inhibitors, were utilized to imitate the deficiency of DNA repair capacity at the incision and ligation steps of NER. Lymphocytes from each donor were divided into three grougs: UVC group, UVC plus NOV group, and UVC plus APC group. DNA single strand breaks were detected in UVC irradiated cells incubated for 0, 30, 60, 90, 120, 180, and 240 min after UVC irradiation. DNA repair rate (DRR) served as an indicator of DNA repair capacity. Results The results indicated that the maximum DNA damage (i.e. maximum tail length) in the UVC group mainly appeared at 90 min. The ranges of DRRs in the UVC group were 62.84%-98.71%. Average DRR value was 81.84%. The DRR difference between males and females was not significant (P<0.05). However, the average DRR value in the UVC plus NOV group and the UVC plus APC group was 52.98% and 39.57% respectively, which were significantly lower than that in the UVC group (P<0.01). Conclusion The comet assay is a rapid, simple and sensitive screening test to assess individual DNA repair (NER) capacity. It is suggested that the time to detect DNA single strand breaks in comet assay should include 0 (before UV irradiation), 90 and 240 min after exposure to 1.5 J·m-2 UVC at least. The DRR, as an indicator, can represent the individual DNA repair capacity in comet assay.
文摘随着“智能油田”的建设加快,构建基于海量石油数据的智能分析系统意义重大。然而,由于石油生产过程中产生的文本数据往往无结构且类型多样,从中抽取关键信息进行分析成为一个研究热点,而信息抽取又需要高质量的语义实体做支撑。根据这一特定问题,提出基于命名实体识别(Named Entity Recognition,NER)技术针对石油非结构化文本进行信息抽取,构建双向长短时记忆(Bidirectional Long Short-Term Memory,Bi LSTM)网络模型提取语料特征,并结合条件随机场(Conditional Random Field,CRF)做分类器,构建了基于Bi LSTM+CRF的高精度NER模型,针对石油工业领域的非结构化文本进行命名实体抽取。通过在修井作业文本数据集上进行对比实验表明,本方法具有较高的精确率和召回率。
文摘We investigated bending moment resistance under diagonal compression load of corner doweled joints with plywood members. Joint members were made of 11-ply hardwood plywood of 19 mm thickness. Dowels were fabricated of Beech and Hornbeam species. Their diameters(6, 8 and 10 mm) and depths of penetration(9, 13 and 17 mm) in joint members were chosen variables in our experiment. By increasing the connector's diameter from 6 to 8 mm, the bending moment resistance under diagonal compressive load was increased, while it decreased when the diameter was increased from 8 to 10 mm. The bending moment resistance under diagonal compressive load was increased by increasing the dowel's depth of penetration. Joints made with dowels of Beech had higher resistance than dowels of Hornbeam. Highest resisting moment(45.18 N·m) was recorded for joints assembled with 8 mm Beech dowels penetrating 17 mm into joint members Lowest resisting moment(13.35 N·m) was recorded for joints assembled with 6 mm Hornbeam dowels and penetrating 9 mm into joint members.
基金the National Natural Science Foundation of China,No.81160395
文摘Carbon nanotubes can carry protein into cells to induce biological effects. Amino-functionalized carbon nanotubes are soluble and biocompatible, have high reactivity and low toxicity, and can help promote nerve cell growth. In this study, amino-functionalized ethylenediamine-treated multi-walled carbon nanotubes were used to prepare carbon nanotubes-nerve growth factor complexes by non-covalent grafting. The physicochemical properties, cytotoxicity to PC12 and chick embryo dorsal root ganglion, and biological activity of the carbon nanotubes-nerve growth factor complexes were investigated. The results showed that amino functionalization improved carbon nanotubes-nerve growth factor complex dispersibility, reduced their toxicity to PC12 cells, and promoted PC12 cell differentiation and chick embryo dorsal root ganglion.
