Objective:To explore the feasibility of establishing an animal model of chronic radiationinduced lung injury.Methods:Twenty-eight New Zealand white rabbits were randomly divided into 3 groups(the right lung irradiatio...Objective:To explore the feasibility of establishing an animal model of chronic radiationinduced lung injury.Methods:Twenty-eight New Zealand white rabbits were randomly divided into 3 groups(the right lung irradiation group,the whole lung irradiation group and the control group).Animal model of radiation-induced lung injury was established b) highdoes radiotherapy in the irradiation groups,then all rabbits underwent CT and pathological examinations at 1.2.4.8.12.16 weeks,respectively after radiation.Results:Within 4 weeks of irradiation,some rabbits in the right lung irradiation group and whole lung irradiation group died. CT and pathological examinations all showed acute radiation pneumonitis.At 8-12 weeks after irradiation,CT scanning showed ground glass samples signs,patchy shadows and fibrotic stripes. Pathological examination showed the fibrosis pulmonary alveolar wall thickened obviously. Conclusions:The clinical animal model of chronic radiation-induced lung injury which corresponds to practical conditions in clinic can be successfully established.展开更多
The effects of dietary lysine on production performance,serum concentrations of metabolites,growth hormone (GH),insulin-like growth factor-I (IGF-I) and IGF-I mRNA expression in growing rabbits were examined.One hundr...The effects of dietary lysine on production performance,serum concentrations of metabolites,growth hormone (GH),insulin-like growth factor-I (IGF-I) and IGF-I mRNA expression in growing rabbits were examined.One hundred weaned New Zealand rabbits were allocated to individual cages and randomly offered a diet containing 5.5 (L1),6.5 (L2),7.5 (L3),8.5 (L4),or 9.5 g (L5) lysine per kg diet.The results showed that the average daily gain (ADG) of the rabbits from L3,L4 or L5 was higher than those from L1 or L2 (P < 0.05).The feed gain ratio (F/G) in the rabbits from L4 or L5 was lower than those from L1 or L2 (P < 0.05).Dietary lysine did not affect serum concentrations of total protein (TP),glucose,insulin (INS),and growth hormone (GH) (P > 0.05).The quadratic effects of lysine on the serum urea nitrogen (SUN) concentration was detected (P = 0.035).Serum IGF-I concentrations had a trend to increase quadratically with the increasing dietary lysine (P = 0.07).A significant correlation was found between serum IGF-I concentrations (x,ng mL-1) and ADG (y,g kg-1): y = -0.017x2 + 1.984x + 20.87 (R2 = 0.8982,P = 0.003).The relative abundance of hepatic and muscular IGF-I mRNA tended to increase with increasing dietary lysine levels (P = 0.053 and 0.082,respectively).Providing the diets mainly consisted of corn,wheat bran and peanut vine,the most appropriate dietary lysine level for growing meat rabbits from weaning to 70 d old was found to be 8.5 g kg-1,and IGF-I may be an important factor controlling growth of weaned rabbits.展开更多
The open reading frame(ORF)of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1to construct the recombinant plasmid pGEX-4T-1-hAPOA1,which was then transformed into Escherichiacolistrain BL21.The ex...The open reading frame(ORF)of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1to construct the recombinant plasmid pGEX-4T-1-hAPOA1,which was then transformed into Escherichiacolistrain BL21.The expression of target fusion protein was induced with isopropylβ-D-1-thiogalactopyranoside(IPTG).The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay(ID-ELISA).ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1∶40 000 was produced,which may bring considerable economic benefits.展开更多
In this (modest) study, we developed artificial neural network (ANN) models for predicting body weight using various independent (input) variables in eight-week old New Zealand white purebred and crossbred rabbits. Fr...In this (modest) study, we developed artificial neural network (ANN) models for predicting body weight using various independent (input) variables in eight-week old New Zealand white purebred and crossbred rabbits. From the whole data sets of similar age groups, 75 percent were used to train the neural network model and 25 percent were used to test the effectiveness of the model. Five predictor variables were used viz, breed, sex, heart girth, body length and height at wither as input variables and body weight was considered as dependent variable from the model. The ANN used was multilayer feed forward network with back propagation of error for efficient learning. Our ANN models (with R2 = 0.68 at ten thousand iterations, and R2 = 0.71 one million iterations) performed better than traditional multivariate linear regression (MLR) models (R2 = 0.66) indicating that the ANN models were able to more accurately capture how the variations in input variables explained the variations in body weight. It is concluded that ANN models are more powerful than MLR models in predicting animals’ body weight. Nonetheless, we recognize that fitting an ANN model requires more computation resources than fitting a tradition MLR model but the benefits of its accuracy outweigh any demerit from the associated computation overhead.展开更多
目的探讨超声微泡破碎技术介导增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)基因在兔骨缺损处转染时,不同途径注射质粒微泡混悬液对转染效率及局部组织的影响。方法3月龄新西兰大白兔10只,制备右尺骨骨缺损模型,按照...目的探讨超声微泡破碎技术介导增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)基因在兔骨缺损处转染时,不同途径注射质粒微泡混悬液对转染效率及局部组织的影响。方法3月龄新西兰大白兔10只,制备右尺骨骨缺损模型,按照随机数字表法分为静脉组和断端间组(n=5)。静脉组和断端间组造模后第10天分别经耳缘静脉或骨缺损断端间向兔体内注射携带EGFP基因的质粒微泡混悬液(0.3 ml/kg)。在超声频率1 MHz,超声强度1.0 W/cm^(2),占空比20%条件下,对两组骨缺损部位超声辐照1 min,进行EGFP基因转染。在基因转染后1周时处死兔,于骨缺损处获取标本制作切片,荧光染色观察各组EGFP表达情况。采用病理图像分析软件分析计算平均光密度。HE染色观察断端间软组织病理特点。结果静脉组和断端间组均观察到绿色荧光蛋白表达。断端间组平均光密度高于静脉组,差异有统计学意义(0.0345±0.0028 vs 0.0004±0.0001,P<0.05)。表达绿色荧光蛋白的细胞主要为骨骼肌细胞,各组未见细胞坏死征象。结论超声微泡破碎技术介导EGFP基因在兔骨缺损处转染时,其效率受不同途径注射质粒微泡混悬液的影响,骨缺损断端间直接注射优于静脉注射。展开更多
文摘Objective:To explore the feasibility of establishing an animal model of chronic radiationinduced lung injury.Methods:Twenty-eight New Zealand white rabbits were randomly divided into 3 groups(the right lung irradiation group,the whole lung irradiation group and the control group).Animal model of radiation-induced lung injury was established b) highdoes radiotherapy in the irradiation groups,then all rabbits underwent CT and pathological examinations at 1.2.4.8.12.16 weeks,respectively after radiation.Results:Within 4 weeks of irradiation,some rabbits in the right lung irradiation group and whole lung irradiation group died. CT and pathological examinations all showed acute radiation pneumonitis.At 8-12 weeks after irradiation,CT scanning showed ground glass samples signs,patchy shadows and fibrotic stripes. Pathological examination showed the fibrosis pulmonary alveolar wall thickened obviously. Conclusions:The clinical animal model of chronic radiation-induced lung injury which corresponds to practical conditions in clinic can be successfully established.
基金supported by the Agriculture Profession Item (3-52)by the earmarked fund for Modern Agro-Industry Technology Research System of the Ministry of Agriculture of the P.R.China (nycytx-44)
文摘The effects of dietary lysine on production performance,serum concentrations of metabolites,growth hormone (GH),insulin-like growth factor-I (IGF-I) and IGF-I mRNA expression in growing rabbits were examined.One hundred weaned New Zealand rabbits were allocated to individual cages and randomly offered a diet containing 5.5 (L1),6.5 (L2),7.5 (L3),8.5 (L4),or 9.5 g (L5) lysine per kg diet.The results showed that the average daily gain (ADG) of the rabbits from L3,L4 or L5 was higher than those from L1 or L2 (P < 0.05).The feed gain ratio (F/G) in the rabbits from L4 or L5 was lower than those from L1 or L2 (P < 0.05).Dietary lysine did not affect serum concentrations of total protein (TP),glucose,insulin (INS),and growth hormone (GH) (P > 0.05).The quadratic effects of lysine on the serum urea nitrogen (SUN) concentration was detected (P = 0.035).Serum IGF-I concentrations had a trend to increase quadratically with the increasing dietary lysine (P = 0.07).A significant correlation was found between serum IGF-I concentrations (x,ng mL-1) and ADG (y,g kg-1): y = -0.017x2 + 1.984x + 20.87 (R2 = 0.8982,P = 0.003).The relative abundance of hepatic and muscular IGF-I mRNA tended to increase with increasing dietary lysine levels (P = 0.053 and 0.082,respectively).Providing the diets mainly consisted of corn,wheat bran and peanut vine,the most appropriate dietary lysine level for growing meat rabbits from weaning to 70 d old was found to be 8.5 g kg-1,and IGF-I may be an important factor controlling growth of weaned rabbits.
基金Supported by the Agricultural Science Independent Innovation Fund Jiangsu Province[CX(16)1326]
文摘The open reading frame(ORF)of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1to construct the recombinant plasmid pGEX-4T-1-hAPOA1,which was then transformed into Escherichiacolistrain BL21.The expression of target fusion protein was induced with isopropylβ-D-1-thiogalactopyranoside(IPTG).The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay(ID-ELISA).ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1∶40 000 was produced,which may bring considerable economic benefits.
文摘In this (modest) study, we developed artificial neural network (ANN) models for predicting body weight using various independent (input) variables in eight-week old New Zealand white purebred and crossbred rabbits. From the whole data sets of similar age groups, 75 percent were used to train the neural network model and 25 percent were used to test the effectiveness of the model. Five predictor variables were used viz, breed, sex, heart girth, body length and height at wither as input variables and body weight was considered as dependent variable from the model. The ANN used was multilayer feed forward network with back propagation of error for efficient learning. Our ANN models (with R2 = 0.68 at ten thousand iterations, and R2 = 0.71 one million iterations) performed better than traditional multivariate linear regression (MLR) models (R2 = 0.66) indicating that the ANN models were able to more accurately capture how the variations in input variables explained the variations in body weight. It is concluded that ANN models are more powerful than MLR models in predicting animals’ body weight. Nonetheless, we recognize that fitting an ANN model requires more computation resources than fitting a tradition MLR model but the benefits of its accuracy outweigh any demerit from the associated computation overhead.
文摘目的探讨超声微泡破碎技术介导增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)基因在兔骨缺损处转染时,不同途径注射质粒微泡混悬液对转染效率及局部组织的影响。方法3月龄新西兰大白兔10只,制备右尺骨骨缺损模型,按照随机数字表法分为静脉组和断端间组(n=5)。静脉组和断端间组造模后第10天分别经耳缘静脉或骨缺损断端间向兔体内注射携带EGFP基因的质粒微泡混悬液(0.3 ml/kg)。在超声频率1 MHz,超声强度1.0 W/cm^(2),占空比20%条件下,对两组骨缺损部位超声辐照1 min,进行EGFP基因转染。在基因转染后1周时处死兔,于骨缺损处获取标本制作切片,荧光染色观察各组EGFP表达情况。采用病理图像分析软件分析计算平均光密度。HE染色观察断端间软组织病理特点。结果静脉组和断端间组均观察到绿色荧光蛋白表达。断端间组平均光密度高于静脉组,差异有统计学意义(0.0345±0.0028 vs 0.0004±0.0001,P<0.05)。表达绿色荧光蛋白的细胞主要为骨骼肌细胞,各组未见细胞坏死征象。结论超声微泡破碎技术介导EGFP基因在兔骨缺损处转染时,其效率受不同途径注射质粒微泡混悬液的影响,骨缺损断端间直接注射优于静脉注射。
