Objective:To observe and compare the curative effect between the intrapleural-chemotherapy combined Nocardia rubra cell wall skeleton-injection and the Nocardia rubra cell wall skeleton-injection after control of the ...Objective:To observe and compare the curative effect between the intrapleural-chemotherapy combined Nocardia rubra cell wall skeleton-injection and the Nocardia rubra cell wall skeleton-injection after control of the malignant pleural effusion by chemotherapy.Methods:Every time after elimination of pleural effusion,we injected DDP and Nocardia rubra cell wall skeleton at the same time into pleural cavity;or after we emplaced an improved central vena catheter into pleural cavity then took out closed-drainage or eliminated the pleural effusion every day,then injected 5-FU 0.5 qd intrapleural-space.After the malignant pleural effusion had being controlled,800μg Nocardia rubra cell wall skeleton was injected intrapleural-space. (We call it as"improved following-therapy").Results:Using the improved following-therapy,the control rate of malignant pleural effusion increased:CR+PR,79.07%>65.79%(P<0.05),and the incidence rate of the encapsulated pleural effusion that would impact on patient’s respiratory faction decreased.Conclusion:Giving chemotherapy after closed-drainage or immediately after taking out pleural effusion everyday,then giving biotherapy after pleural effusion had been controlled,the curative effect would be enhanced and the side-reaction would be reduced.展开更多
Diabetic foot ulcers are one of the most severe complications of diabetes that have imposed great financial and psychological burdens on diabetic patients.A Nocardia rubra cell wall skeleton(Nr-CWS)can be externally a...Diabetic foot ulcers are one of the most severe complications of diabetes that have imposed great financial and psychological burdens on diabetic patients.A Nocardia rubra cell wall skeleton(Nr-CWS)can be externally applied to accelerate wound healing.However,its clinical application has not yet been reported.Herein,we report two patients with diabetic foot ulcers treated with Nr-CWS.After wound debridement,the wound was covered with a sterile cotton ball infiltrated with an Nr-CWS that was diluted with 2.0 mL of saline.The covers were changed every two days until complete wound healing occurred.The two wounds healed after 3 and 12 weeks,respectively.This article aims to provide a new treatment for diabetic foot ulcers,with the hope that physicians may consider an Nr-CWS as a complementary method for the treatment of chronic wounds.展开更多
Mesenchymal stem cell(MSC)-derived exosomes(Exos)were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect.MSCs pretreated with chemistry or biology factors wer...Mesenchymal stem cell(MSC)-derived exosomes(Exos)were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect.MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes.Hence,this study was designed to explore whether exosomes derived from human umbilical cord MSCs(hucMSCs)preconditioned with Nocardia rubra cell wall skeleton(Nr-CWS)exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms.The results showed that Nr-CWS-Exos facilitated the proliferation,migration and tube formation of endothelial cells in vitro.In vivo,Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos.Furthermore,the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos.CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cells via the miR-4782-5p/VEGFA axis.Taken together,those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.展开更多
文摘Objective:To observe and compare the curative effect between the intrapleural-chemotherapy combined Nocardia rubra cell wall skeleton-injection and the Nocardia rubra cell wall skeleton-injection after control of the malignant pleural effusion by chemotherapy.Methods:Every time after elimination of pleural effusion,we injected DDP and Nocardia rubra cell wall skeleton at the same time into pleural cavity;or after we emplaced an improved central vena catheter into pleural cavity then took out closed-drainage or eliminated the pleural effusion every day,then injected 5-FU 0.5 qd intrapleural-space.After the malignant pleural effusion had being controlled,800μg Nocardia rubra cell wall skeleton was injected intrapleural-space. (We call it as"improved following-therapy").Results:Using the improved following-therapy,the control rate of malignant pleural effusion increased:CR+PR,79.07%>65.79%(P<0.05),and the incidence rate of the encapsulated pleural effusion that would impact on patient’s respiratory faction decreased.Conclusion:Giving chemotherapy after closed-drainage or immediately after taking out pleural effusion everyday,then giving biotherapy after pleural effusion had been controlled,the curative effect would be enhanced and the side-reaction would be reduced.
基金This study was sponsored by the National Natural Science Foundation of China(grant no.82172224).
文摘Diabetic foot ulcers are one of the most severe complications of diabetes that have imposed great financial and psychological burdens on diabetic patients.A Nocardia rubra cell wall skeleton(Nr-CWS)can be externally applied to accelerate wound healing.However,its clinical application has not yet been reported.Herein,we report two patients with diabetic foot ulcers treated with Nr-CWS.After wound debridement,the wound was covered with a sterile cotton ball infiltrated with an Nr-CWS that was diluted with 2.0 mL of saline.The covers were changed every two days until complete wound healing occurred.The two wounds healed after 3 and 12 weeks,respectively.This article aims to provide a new treatment for diabetic foot ulcers,with the hope that physicians may consider an Nr-CWS as a complementary method for the treatment of chronic wounds.
基金the National Natural Science Foundation of Jiangsu Province(No.BK20201155)the Foundation of Xuzhou Key Medical Talents(No.XWRCHT-20220067).
文摘Mesenchymal stem cell(MSC)-derived exosomes(Exos)were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect.MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes.Hence,this study was designed to explore whether exosomes derived from human umbilical cord MSCs(hucMSCs)preconditioned with Nocardia rubra cell wall skeleton(Nr-CWS)exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms.The results showed that Nr-CWS-Exos facilitated the proliferation,migration and tube formation of endothelial cells in vitro.In vivo,Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos.Furthermore,the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos.CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cells via the miR-4782-5p/VEGFA axis.Taken together,those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.
