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Sevoflurane and nitric oxide synthase expression in rat cochlea
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作者 Yuantao Li Qingzhong Hou +5 位作者 Mingguang Wu Xiaolei Huang Jun Cao Yin Gu Xiaofei Qi Yawen Li 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第19期1505-1509,共5页
Sevoflurane exhibits anesthetic action by inhibiting the auditory cortex, brain stem nitric oxide synthase activity, and reducing nitric oxide (NO), thereby interfering with the hearing process. However, the influen... Sevoflurane exhibits anesthetic action by inhibiting the auditory cortex, brain stem nitric oxide synthase activity, and reducing nitric oxide (NO), thereby interfering with the hearing process. However, the influence of sevoflurane on peripheric receptor (cochlea) NO remains poorly understood. Results from the present study showed that sevoflurane downregulated cochlear inducible NO synthase, endothelial NO synthase and neuronal NO synthase expression in a dose dependent manner. This suggests that sevoflurane can decrease cochlear NO synthase expression in a dose dependent manner. 展开更多
关键词 SEVOFLURANE cochlea nitric oxide synthase nitric oxide rats
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Expression of inducible nitric oxide synthase and cyclooxygenase-2 in pancreatic adenocarcinoma:Correlation with microvessel density 被引量:14
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作者 Hans U.Kasper Hella Wolf +2 位作者 Uta Drebber Helmut K.Wolf Michael A.Kern 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第13期1918-1922,共5页
AIM:Cydooxygenases (COX) are key enzymes for conversion of arachidonic acid to prostaglandins.Nitric oxide synthase (NOS) is the enzyme responsible for formation of nitric oxide. Both have constitutive and inducible i... AIM:Cydooxygenases (COX) are key enzymes for conversion of arachidonic acid to prostaglandins.Nitric oxide synthase (NOS) is the enzyme responsible for formation of nitric oxide. Both have constitutive and inducible isoforms.The inducible isoforms (iNOS and COX-2) are of great interest as regulators of tumor angiogenesis,tumorigenesis and inflammatory processes.This study was to clarify their role in pancreatic adenocarcinomas. METHODS:We investigated the immunohistochemical iNOS and COX-2 expression in 40 pancreatic ductal adenocardnomas of different grade and stage.The results were compared with microvessel density and dinicopathological data. RESULTS:Twenty-one (52.5%) of the cases showed iNOS expression,15 (37.5%) of the cases were positive for COX-2. The immunoreaction was heterogeneously distributed within the tumors.Staining intensity was different between the tumors.No correlation between iNOS and COX-2 expression was seen.There was no relationship with microvessel density. However,iNOS positive tumors developed more often distant metastases and the more malignant tumors showed a higher COX-2 expression.There was no correlation with other clinicopathological data. CONCLUSION:Approximately half of the cases expressed iNOS and COX-2.These two enzymes do not seem to be the key step in angiogenesis or carcinogenesis of pancreatic adenocarcinomas.Due to a low prevalence of COX-2 expression,chemoprevention of pancreatic carcinomas by COX-2 inhibitors can only achieve a limited success. 展开更多
关键词 Adenocarcinoma Aged Aged 80 and over Cyclooxygenase 2 Female Humans immunohistochemistry ISOENZYMES Male Membrane Proteins MICROCIRCULATION Middle Aged nitric oxide synthase nitric oxide synthase Type II Pancreas Pancreatic Neoplasms Prostaglandin-Endoperoxide synthases
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Distribution of constitutive nitric oxide synthase in the jejunum of adult rat 被引量:6
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作者 ChenYM QianZM 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第3期537-539,共3页
AIM: To study the distribution of the constitutive nitric oxide synthase (NOS) in the jejunum of adult rat. METHODS: The distribution of endothelial NOS (eNOS) was detected by immunohistochemistry. Immunofluorescence ... AIM: To study the distribution of the constitutive nitric oxide synthase (NOS) in the jejunum of adult rat. METHODS: The distribution of endothelial NOS (eNOS) was detected by immunohistochemistry. Immunofluorescence histochemical dual staining technique were used for studying the distribution of neuronal NOS (nNOS) and eNOS. The dual stained slides were observed under a confocal laser scanning microscope. RESULTS: Positive neuronal NOS (nNOS) and endothelial NOS (eNOS) cells were found to be distributed in lamina propria of villi, and the epithelial cell was not stained. eNOS was mainly located in submucosal vascular endothelia, while nNOS was mainly situated in myenteric plexus. Some cells in the villi had both nNOS and eNOS. More than 80% of the cells were positive for both nNOS and eNOS, the rest cells were positive either for nNOS or for eNOS. CONCLUSION: The two constitutive nitric oxide synthases are distributed differently in the jejunum of rat. nNOS distributed in myenteric plexus is a neurotransmitter in the non-adrenergic non-cholinergic (NANC) inhibitory nerves. eNOS distributed in endothelial and smooth muscle cells of blood vessels plays vasodilator role. eNOS and nNOS are coexpressed in some cells of lamina propria of villi. NO generated by those NOS is very important in the physiological and pathological process of small intestine. 展开更多
关键词 Animals immunohistochemistry JEJUNUM Male nitric oxide synthase nitric oxide synthase Type I nitric oxide synthase Type III RATS Rats Sprague-Dawley Research Support Non-U.S. Gov't Tissue Distribution
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Distribution of nitric oxide synthase in stomach myenteric plexus of rats 被引量:11
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作者 Xi Peng Jin-Bin Feng Hong Yan Yun Zhao Shi-Liang Wang Institute of Burn Research,Southwest Hospital,Third Military Medical University,Chongqing 400038,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第6期852-854,共3页
AIM: To study the distribution of nitric oxide synthase (NOS) in rat stomach myenteric plexus. METHODS: The distribution of NOS in gastric wall was studied in quantity and location by the NADPH-diaphorase (NDP) histoc... AIM: To study the distribution of nitric oxide synthase (NOS) in rat stomach myenteric plexus. METHODS: The distribution of NOS in gastric wall was studied in quantity and location by the NADPH-diaphorase (NDP) histochemical staining method and whole mount preparation technique. RESULTS: NOS was distributed in whole stomach wall, most of them were located in myenteric plexus, and distributed in submucosal plexus.The shape of NOS positive neurons was basically similar, most of them being round and oval in shape. But their density, size and staining intensity varied greatly in the different parts of stomach. The density was 62+/-38 cells mm(2) (antrum), 43+/-32 cells/mm(2) (body), and 32+/-28 cells mm(2) (fundus), respectively. The size and staining intensity of NOS positive neurons in the fundus were basically the same, the neurons being large and dark stained, while they were obviously different in antrum. In the body of the stomach, the NOS positive neurons were in an intermediate state from fundus to antrum. There were some beadlike structures which were strung together by NOS positive varicosities in nerve fibers, some were closely adherent to the outer walls of blood vessels. CONCLUSION: Nitric oxide might be involved in the modulation of motility, secretion and blood circulation of the stomach, and the significant difference of NOS positive neurons in different parts of stomach myenteric plexus may be related to the physiologic function of stomach. 展开更多
关键词 ANIMALS FEMALE Myenteric Plexus nitric oxide synthase RATS Rats Wistar STOMACH Tissue Distribution
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Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual-and auditory-related nervous tissues, in a macaque model of type 2 diabetes 被引量:2
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作者 Qihui Luo Wentao Liu +4 位作者 Jingyao Chen Mingshu Wang Wen Zeng Zhengli Chen Anchun Cheng 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第4期302-307,共6页
The present study detected distribution and expression of nerve growth factor and inducible nitric oxide synthase in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a ma... The present study detected distribution and expression of nerve growth factor and inducible nitric oxide synthase in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 diabetes using immunohistochemistry. Results showed that nerve growth factor expression decreased, but inducible nitric oxide synthase expression increased, in the mesencephalon and diencephalon, as well as visual- and auditory- related nervous tissues. These results suggested that nerve growth factor and inducible nitric oxide synthase play an important role in regulating the development of diabetic visual- and auditory-related diseases. 展开更多
关键词 DIENCEPHALON immunohistochemistry inducible nitric oxide synthase MESENCEPHALON nerve growth factor neural regeneration optic nerve type 2 diabetes
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Spatio-temporal expression of inducible nitric oxide synthase in and surrounding a region of rat frontal lobe damaged with a sharp instrument
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作者 Zhixian He Zhijun Zhang +3 位作者 Yulin Dong Guangming Lü Ting Wang Hengjian Ni 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第2期123-128,共6页
BACKGROUND: Inducible nitric oxide synthase (iNOS) cannot be detected in the neurons and glial cells of normal rats, but iNOS can be found in some neurons and glial cells of rats following ischemic, traumatic, neur... BACKGROUND: Inducible nitric oxide synthase (iNOS) cannot be detected in the neurons and glial cells of normal rats, but iNOS can be found in some neurons and glial cells of rats following ischemic, traumatic, neurotoxic or inflammatory damage. OBJECTIVE: To investigate iNOS expression and iNOS-positive cell types at various time points following damage to the rat frontal lobe using a sharp instrument. DESIGN: A nerve molecular biology, randomized, controlled study. TIME AND SETTING: This experiment was performed at the Department of Human Anatomy, Institute of Neurobiology, Medical School of Nantong University, between April 2006 and December 2007. MATERIALS: Rabbit anti-iNOS antibody (Santa Cruz, USA), biotin labeled goat anti-rabbit antibody (Sigma, USA), reverse transcription kit (Biouniquer, Hong Kong, China) and horseradish peroxidase labeled goat anti-rabbit antibody (Pierce, USA) were used for this study. METHODS: A total of 112 healthy rats aged 3 months were randomly assigned into a sham operation group (n = 28) and a damage group (n = 84). Rat models of frontal lobe damage were induced in the damage group using a sharp instrument to make an incision in the frontal lobe cortex. In the sham operation group, the rat bone window was opened but brain tissues were left intact. MAIN OUTCOME MEASURES: Parameters were measured at 3, 6, 12, 24, 72, 120 and 168 hours following damage in both groups. Pathological changes were observed using Nissl staining and hematoxylin-eosin staining. Expression of iNOS mRNA, iNOS protein and iNOS-positive cells were examined by RT-PCR, Western blot analysis and immunohistochemistry, respectively. RESULTS: A large number of inflammatory cells infiltrated the damaged region 12 and 24 hours following damage, iNOS mRNA and iNOS protein expression increased in and around the damaged region 3 hours following damage, reached a peak at 24 hours, and then gradually decreased. The changes in iNOS-positive cell number reflected the changes in iNOS mRNA and iNOS protein expression after damage, iNOS was mainly found in neural cells at 3 and 6 hours, in macrophages at 12 and 24 hours, and in glial cells at 72 and 120 hours after damage. iNOS-positive cells were few in and surrounding the damaged region at 168 hours. There were a few iNOS-positive neural cells in the rat frontal lobe cortex in the sham operation group. CONCLUSION: Neurons, macrophages and glial cells can express iNOS following rat frontal lobe damage caused by a sharp instrument. The levels of iNOS expression, and the cell types expressing iNOS, change with time. 展开更多
关键词 frontal lobe damage inducible nitric oxide synthase RT-PCR WESTERN-BLOT immunohistochemistry rats
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COLOCALIZATION OF NITRIC OXIDE SYNTHASE IN GNRH NEURONS OF RAT FOREBRAIN AND HYPOTHALAMUS
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作者 Zhou Jinsong,Song Tianbao Department of Histology & Embryology, Xi′an Medical University, Xi′an 710061 《Journal of Pharmaceutical Analysis》 CAS 1999年第2期108-111,146,共5页
In order to gain more information about the effect of nitric oxide (NO) on GnRH release, double NADPH diaphorase histochemistry GnRH immunohistochemistry staining was used to investigate the morphological relationsh... In order to gain more information about the effect of nitric oxide (NO) on GnRH release, double NADPH diaphorase histochemistry GnRH immunohistochemistry staining was used to investigate the morphological relationship between NO synthase (NOS) containing cells and GnRH neurons in the forebrain and hypothalamus of rats. The results showed that some of the GnRH neurons in the diagonal band of Broca, olfactory tubercle and deeper part of temporal cortex had the NOS activity, suggesting GnRH secretion can be rapidly regulated by NO derived from GnRH neurons themselves in an autocrine manner. 展开更多
关键词 nitric oxide synthase gonadotropin releasing hormone neuron immunohistochemistry RAT
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Expression of differential nitric oxide synthase isoforms in human gastric mucosa infected with Helicobacter pylori
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作者 屠振兴 龚燕芳 +3 位作者 丁华 许国铭 李兆申 满晓华 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第5期286-289,共4页
Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33... Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H.pylori infection was confirmed by Giems staining and bacteria culture under microaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: (1) The positive rate of H. pylori infection was 66.7%(22/33). (2) iNOS positive staining in inflammatory cells was detected in 77.3%(17/22) of samples with H.pylori and 27.3%(3/11) without H.pylori infection (P<0.01). (3) eNOS expression in inflammatory cells was found in 77.3%(17/22) of samples with H. pylori and 18.2%(2/11) without H.pylori infection (P<0.01). (4) Nitrotyrosine expression in inflammatory cells was observed in 59.1%(13/22) of samples with H. pylori and 54.5%(6/11) without H. pylori infection (P>0.05). (5) Moderate and severe infiltrations of inflammatory cells were found in 86.4%(19/22) of gastric biopsies with H. pylori and 9.1%(1/11) of samples without H. pylori infection (P<0.01). Conclusion: H.pylori infection might promote infiltration of mononuclear cells and macrophages in gastric mucosa and induce iNOS expression in these cells. The accumulated nitric oxide in local area may result in gastric mucosa damage. 展开更多
关键词 H. pylori nitric oxide synthase immunohistochemistry
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Immunohistochemical localization of nitric oxide synthase in gastrointestinal tract of rats
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作者 高瞻 朱运龙 +2 位作者 梅琦 陈健康 黄威权 《Journal of Medical Colleges of PLA(China)》 CAS 1998年第2期150-152,共3页
Objective: To investigate the distribution of the three subtypes of nitric oxide synthase (NOS) inthe gastrointestinal tract of rats. Methods: Immunohistochemical ABC methods were used. Results: Neuronal NOS(nNOS)-pos... Objective: To investigate the distribution of the three subtypes of nitric oxide synthase (NOS) inthe gastrointestinal tract of rats. Methods: Immunohistochemical ABC methods were used. Results: Neuronal NOS(nNOS)-positive substance was localized in epithelial cells, endocrine cells, endothelial cells andneuronal cell bodies in the intermuscular plexus and submucous plexus. Inducible NOS(iNOS)-positive substance was located in smooth muscle cells in gastric corpus, intestinal mucosa epithelium and endocrine cells.Endothelial NOS(eNOS)-positive substance was distributed mainly in vascular endothelial cells. Conclusion:The distribution patterns of the three subtypes of NOS exhibited specificity and overlapping profile in the gastrointestinal tract of rats. 展开更多
关键词 nitric oxide synthase immunohistochemistry GASTROINTESTINAL TRACT rat
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丹参注射液对庆大霉素耳中毒豚鼠耳蜗NOS活性的影响 被引量:7
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作者 王爱梅 汤浩 +2 位作者 沈静 方秀斌 王铁民 《中国医科大学学报》 CAS CSCD 北大核心 2001年第2期85-87,共3页
目的 :探讨丹参注射液 (SalviaMiltiorrhiza ,SM)对庆大霉素 (gentamicin ,GM)耳毒性损伤的保护作用。方法 :制备豚鼠药物中毒性耳聋模型 ,应用NADPH 黄递酶 (NADPH diaphorase,NADPH d)组织化学染色及图像分析技术 ,并结合听性脑干反应... 目的 :探讨丹参注射液 (SalviaMiltiorrhiza ,SM)对庆大霉素 (gentamicin ,GM)耳毒性损伤的保护作用。方法 :制备豚鼠药物中毒性耳聋模型 ,应用NADPH 黄递酶 (NADPH diaphorase,NADPH d)组织化学染色及图像分析技术 ,并结合听性脑干反应 (auditorybrainstemresponse ,ABR)测试 ,观察SM对GM耳中毒豚鼠耳蜗一氧化氮合酶 (ni tricoxidesynthase ,NOS)活性的影响及其与听阈的关系。结果 :SM +GM组耳蜗NOS活性和ABR阈值均明显低于GM组 (P <0 .0 1) ;且ABR阈值与NOS活性变化高度相关 (rGM=- 0 .82 36~ - 0 .86 6 2 ;rSM +GM=- 0 .86 2 8~- 0 .9172 ,P <0 .0 1)。结论 :SM可通过降低耳蜗NOS活性以减轻GM的耳毒性损伤 ,从而改善听功能。 展开更多
关键词 丹参注射液 庆大霉素 一氧化氮合酶 耳蜗 豚鼠 实验研究 药物中毒性耳聋
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诱导型一氧化氮合酶对肺纤维化形成的促进作用 被引量:19
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作者 陈晓玲 李英敏 +2 位作者 黄善生 王殿华 李文斌 《中国病理生理杂志》 CAS CSCD 北大核心 2002年第10期1251-1253,T005,共4页
目的 :研究纤维化肺内诱导型一氧化氮合酶上调及其与肺纤维化形成的关系。方法 :气管内滴注平阳霉素 (BLMA55mL/kg) ,观察注后 7、14、30d肺组织诱导型一氧化氮合酶 (iNOS)阳性细胞数和Ⅰ、Ⅲ型胶原纤维的变化 ;用氨基胍 (AG)阻断iNOS合... 目的 :研究纤维化肺内诱导型一氧化氮合酶上调及其与肺纤维化形成的关系。方法 :气管内滴注平阳霉素 (BLMA55mL/kg) ,观察注后 7、14、30d肺组织诱导型一氧化氮合酶 (iNOS)阳性细胞数和Ⅰ、Ⅲ型胶原纤维的变化 ;用氨基胍 (AG)阻断iNOS合成NO后 ,观察出肺血中NO-2 /NO-3 和肺组织中羟脯氨酸含量以及肺组织形态结构的变化。结果 :①注BLMA57d、14d和 30d组大鼠肺间质iNOS阳性细胞数明显多于对照组 (P <0 .0 1) ,并且 ,BLMA57d组和BLMA514d组还多于BLMA530d组 (P <0 .0 1)。BLMA514d和 30d组大鼠肺间质胶原纤维的出现多于对照组 ,BLMA514d组以Ⅲ型胶原纤维增多为主 ,BLMA530d组以Ⅰ型胶原纤维增多为主。②AG缓解出肺血NO-2 /NO-3和肺组织中羟脯氨酸含量的升高 ;AG还阻止肺间质或纤维细胞和巨噬细胞的增多。结论 :在肺纤维化形成过程中 ,肺内iNOS上调 ,大量生成NO ,有促肺纤维化的作用。 展开更多
关键词 肺纤维化 一氧化氮合酶 免疫组织化学 大鼠
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丹参注射液对庆大霉素耳中毒豚鼠耳蜗血管纹NOS活性的影响 被引量:9
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作者 王爱梅 汤浩 +2 位作者 沈静 崔桂英 崔城 《中国应用生理学杂志》 CAS CSCD 北大核心 2001年第1期89-92,共4页
目的 :研究丹参注射液 (SM )对庆大霉素 (GM)耳中毒豚鼠耳蜗血管纹一氧化氮合酶 (NOS)活性的影响及其与听阈的关系 ,探讨SM对GM耳毒性损伤的保护作用。方法 :应用NADPH 黄递酶 (NADPH d)组织化学染色及图象分析技术 ,并结合听性脑干反应... 目的 :研究丹参注射液 (SM )对庆大霉素 (GM)耳中毒豚鼠耳蜗血管纹一氧化氮合酶 (NOS)活性的影响及其与听阈的关系 ,探讨SM对GM耳毒性损伤的保护作用。方法 :应用NADPH 黄递酶 (NADPH d)组织化学染色及图象分析技术 ,并结合听性脑干反应 (ABR)测试。结果 :SM +GM组耳蜗血管纹NOS活性和ABR阈值均明显低于GM组 (P <0 .0 1) ;且各组NOS活性变化与ABR阈移高度相关 (rcontrol=- 0 .946 4;rGM =- 0 .9117;rSM +GM =- 0 .895 8,P <0 .0 1)。结论 :SM可通过降低耳蜗血管纹NOS活性以减轻GM的耳毒性损伤 。 展开更多
关键词 丹参注射液 庆大霉素 一氧化氮合酶 耳蜗 肿毒
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诱生型一氧化氮合成酶在豚鼠噪声损伤耳蜗中的表达 被引量:4
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作者 熊敏 汪建 +3 位作者 何青莲 邓恒山 尤景敏 梁萍 《广东医学》 CAS CSCD 2002年第6期569-570,共2页
目的 观察诱生型一氧化氮合成酶 (iNOS)在噪声暴露豚鼠耳蜗中的表达。方法 将研究组豚鼠暴露于白噪声造成噪声性聋的模型 ,耳蜗用石蜡包埋、切片 ,用免疫组织化学的方法观察iNOS在耳蜗的表达。结果 iNOS在噪声暴露豚鼠耳蜗中表达阳... 目的 观察诱生型一氧化氮合成酶 (iNOS)在噪声暴露豚鼠耳蜗中的表达。方法 将研究组豚鼠暴露于白噪声造成噪声性聋的模型 ,耳蜗用石蜡包埋、切片 ,用免疫组织化学的方法观察iNOS在耳蜗的表达。结果 iNOS在噪声暴露豚鼠耳蜗中表达阳性 ,以血管纹和螺旋神经节的表达较强。结论 iNOS在噪声暴露豚鼠耳蜗中呈阳性表达 ,提示一氧化氮在噪声性聋的发病机制中起重要作用。 展开更多
关键词 免疫组织化学 诱生型一氧化氮合酶 豚鼠 噪声损伤 耳蜗
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基环氧合酶-2和诱导型一氧化氮合酶在舌不典型增生和鳞癌组织中的表达 被引量:8
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作者 许国雄 陈伟良 +3 位作者 李海刚 李劲松 杨朝晖 潘朝斌 《癌症》 SCIE CAS CSCD 北大核心 2005年第11期1345-1349,共5页
背景与目的:近期研究表明,环氧合酶(cyclooxygenase-2,COX-2)和诱导型一氧化氮合酶(induciblenitricoxidesynthase,iNOS)共同参与肿瘤的发生发展过程,但有关它们异常表达与舌鳞癌生物学行为的关系尚不清楚。本研究拟探讨COX-2和iNOS在... 背景与目的:近期研究表明,环氧合酶(cyclooxygenase-2,COX-2)和诱导型一氧化氮合酶(induciblenitricoxidesynthase,iNOS)共同参与肿瘤的发生发展过程,但有关它们异常表达与舌鳞癌生物学行为的关系尚不清楚。本研究拟探讨COX-2和iNOS在舌鳞癌的表达和两者间的相互关系。方法:应用免疫组化SP法,检测59例舌鳞癌及其45例增生性病变(轻、中、重度不典型增生分别为22例、20例、3例)和36例癌旁正常鳞状上皮中COX-2蛋白、iNOS蛋白的表达情况,并对这些指标进行相关分析。结果:在癌旁正常鳞状上皮、轻、中、重度不典型增生上皮和舌鳞癌组织中,COX-2蛋白异常表达检出率分别为8.3%(3/36)、4.5%(1/22)、5.0%(1/20)、0(0/3)和45.8%(27/59);iNOS蛋白异常表达检出率分别为44.4%(16/36)、72.8%(16/22)、80.0%(16/20)、100.0%(3/3)和98.3%(58/59)。COX-2蛋白在癌旁正常鳞状上皮的表达,与舌鳞癌组织的表达差异有显著性(P<0.001),与不典型增生总体的表达差异无显著性(P>0.05);iNOS蛋白在癌旁正常鳞状上皮的表达,与不典型增生总体和癌组织的表达差异均有显著性(P<0.001)。等级相关分析结果显示,COX-2、iNOS表达异常与组织学分级均显著正相关(相关系数r分别为0.418和0.607,P值均<0.001),而且COX-2蛋白与iNOS蛋白之间也具有显著相关性(r=0.245,P<0.001)。结论:COX-2和iNOS蛋白异常表达与舌鳞癌癌变过程显著相关。 展开更多
关键词 舌肿瘤/病理学 不典型增生 环氧合酶-2 诱导 型一氧化氮合酶 免疫组织化学 诱导型一氧化氮合酶 重度不典型增生 舌鳞癌组织 COX-2蛋白
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栉孔扇贝神经节一氧化氮合酶的组织化学和免疫组化定位 被引量:8
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作者 孙虎山 王宜艳 +2 位作者 王晓安 孙修勤 李光友 《中国水产科学》 CAS CSCD 北大核心 2005年第1期20-25,共6页
采用组织化学和免疫组化技术对栉孔扇贝(Chlamysfarreri)神经节内的一氧化氮合酶(NOS)进行定位研究。组织化学显示,存在NOS的部位如下:脑神经节内纵行的神经纤维和表层的少量小细胞;足神经节表层的大量小细胞,中央大量水平分布的神经纤... 采用组织化学和免疫组化技术对栉孔扇贝(Chlamysfarreri)神经节内的一氧化氮合酶(NOS)进行定位研究。组织化学显示,存在NOS的部位如下:脑神经节内纵行的神经纤维和表层的少量小细胞;足神经节表层的大量小细胞,中央大量水平分布的神经纤维;脏神经节中部大量水平分布的神经纤维,前叶内大量小细胞和神经纤维,后叶内少量小细胞和许多环行神经纤维,侧叶内大量似放射状分布的神经纤维;脑足和脑脏神经索内的神经纤维。免疫组化定位表明,神经型一氧化氮合酶(nNOS)和诱导型一氧化氮合酶(iNOS)在整个神经系统内均呈阴性;足神经节和脏神经节内有少量神经细胞呈内皮型一氧化氮合酶(eNOS)强阳性;各神经节和神经索内的部分小细胞和神经纤维呈eNOS弱阳性。栉孔扇贝进化上为较低等的贝类,NOS阳性神经细胞应主要分布于外周器官组织内,神经系统内大量的NOS可在其神经传导和免疫调节等方面发挥重要的作用。 展开更多
关键词 栉孔扇贝 神经节 一氧化氮合酶 组织化学 免疫组化
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NOS/NO和HO/CO系统及药物干预在实验性兔动脉粥样硬化进程中的作用 被引量:6
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作者 刘同涛 于清霞 +2 位作者 田庆印 孙春丽 李伯勤 《山东大学学报(医学版)》 CAS 北大核心 2008年第6期566-570,共5页
目的探讨动脉粥样硬化进程中一氧化氮合酶/一氧化氮(NOS/NO)和血红素加氧酶/一氧化碳(HO/CO)系统的变化、相互关系以及辛伐他汀对该系统的影响。方法16只家兔予以高胆固醇饮食喂养8周,停用高胆固醇饮食后,随机分为辛伐他汀组(n=8)和模型... 目的探讨动脉粥样硬化进程中一氧化氮合酶/一氧化氮(NOS/NO)和血红素加氧酶/一氧化碳(HO/CO)系统的变化、相互关系以及辛伐他汀对该系统的影响。方法16只家兔予以高胆固醇饮食喂养8周,停用高胆固醇饮食后,随机分为辛伐他汀组(n=8)和模型组(n=8)。辛伐他汀组喂饲辛伐他汀进行药物干预,继续给予普通饲料喂养8周;同时设正常对照组(n=8),给予普通饲料喂养16周。然后取静脉血和主动脉组织,分别用沉淀漂浮酶联法、Chalmers A H、硝酸还原酶法测定各实验组血中TC、TG、LDL、HDL、CO、NO含量,免疫组织化学方法测定主动脉组织中诱导型一氧化氮合酶(iNOS)和血红素加氧酶-1(HO-1)的表达水平;并比较两组间各项参数的差异。结果8周末与对照组比较,模型组血脂水平明显升高,血清NO含量明显降低,血浆CO水平明显升高(P均<0.01)。16周末与模型组比较,辛伐他汀组血浆TC、TG、LDL明显下降(P<0.01),HDL和血清NO含量明显升高(P<0.01),血浆CO水平明显降低(P<0.01),HO-1及iNOS表达明显减少(P<0.01)。结论动脉粥样硬化进程中,HO/CO和NOS/NO系统显示出互补及代偿性调节作用,辛伐他汀可以通过下调HO/CO和NOS/NO系统而延缓动脉粥样硬化进程。 展开更多
关键词 动脉硬化 一氧化氮合酶 血红素加氧酶 一氧化氮 一氧化碳 辛伐他汀 免疫组织化学
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噪声对大鼠学习记忆中海马区NOS阳性神经元表达的影响 被引量:14
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作者 欧阳伟 王家同 +2 位作者 李金声 孙喜庆 任杰 《中国心理卫生杂志》 CSSCI CSCD 北大核心 2003年第2期80-83,共4页
目的 :拟在白噪声下观察对大鼠学习记忆的影响及海马区NOS的变化 ,探讨噪声所致大鼠学习记忆下降的机制。方法 :44只SD大鼠 ,其中 2 0只随机分两组 ,分别在持续 80dB (A)白噪声和无噪声的环境下 ,在Y -迷宫中进行空间辨别学习记忆训练 ... 目的 :拟在白噪声下观察对大鼠学习记忆的影响及海马区NOS的变化 ,探讨噪声所致大鼠学习记忆下降的机制。方法 :44只SD大鼠 ,其中 2 0只随机分两组 ,分别在持续 80dB (A)白噪声和无噪声的环境下 ,在Y -迷宫中进行空间辨别学习记忆训练 ,了解噪声对大鼠学习记忆的影响 ,另 2 4只大鼠随机分三组 :噪声训练组、正常训练组和噪声观察组 ,以同样的方法训练 ,用免疫组化的方法观察持续噪声对海马区NOS阳性神经元表达变化。结果 :噪声能降低大鼠学习记忆能力 ,而且海马区NOS阳性神经元较正常对照组的数量及染色强度显著降低。结论 :噪声降低海马区神经元活性 ,NOS的合成减少 ,抑制海马习得性长时程突触增强 ,影响记忆的获得与保持 。 展开更多
关键词 噪声 大鼠 学习记忆 海马区 神经元 一氧化氮 一氧化氮合酶
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吸入麻醉药诱导c-fos基因和一氧化氮合酶在大鼠中枢神经系统的共同表达 被引量:10
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作者 徐礼鲜 张惠 +2 位作者 马加海 张洪凯 李军 《临床麻醉学杂志》 CAS CSCD 2001年第3期151-153,共3页
目的 本研究旨在探索吸入麻醉药诱导c fos基因和一氧化氮合酶 (NOS)在中枢神经系统共同表达的现象。方法 用免疫组化双标方法 ,在脑切片上观察c fos基因和NOS阳性神经元的分布。结果 吸入麻醉药诱导Fos蛋白在中枢的表达部位及阳性神... 目的 本研究旨在探索吸入麻醉药诱导c fos基因和一氧化氮合酶 (NOS)在中枢神经系统共同表达的现象。方法 用免疫组化双标方法 ,在脑切片上观察c fos基因和NOS阳性神经元的分布。结果 吸入麻醉药诱导Fos蛋白在中枢的表达部位及阳性神经元的密度与以往相同。同一实验条件下安氟醚、异氟醚吸入麻醉组的NOS表达明显少于对照组 (P <0 0 5或 0 0 1) ,无一例c fos与NOS在同一神经元内共同表达的现象。结论 安氟醚和异氟醚吸入麻醉作用与抑制在体大鼠脑内NOS的活性有关。 展开更多
关键词 吸入麻醉 C-FOS基因 一氧化氮合酶 免疫组织化学 大鼠 中枢神经系统
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噪声刺激后豚鼠耳蜗抗氧化能力的变化和α-硫辛酸对声损伤的保护作用 被引量:14
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作者 刁明芳 刘海瑛 +1 位作者 张琰敏 高文元 《生理学报》 CAS CSCD 北大核心 2003年第6期672-676,共5页
实验探讨了声刺激后豚鼠血清总抗氧化能力(total antioxidant capacity,TAC)和耳蜗组织一氧化氮(nitricoxide,NO)含量的变化及α-硫辛酸的抗氧化和对声损伤的保护作用。将豚鼠(350-400 g)随机分为无噪声对照组(n=20)、噪声+生理盐水组(n... 实验探讨了声刺激后豚鼠血清总抗氧化能力(total antioxidant capacity,TAC)和耳蜗组织一氧化氮(nitricoxide,NO)含量的变化及α-硫辛酸的抗氧化和对声损伤的保护作用。将豚鼠(350-400 g)随机分为无噪声对照组(n=20)、噪声+生理盐水组(n=20)和噪声+α-硫辛酸组(n=20)。噪声刺激(4.kHz倍频程,115 dB SPL 5 h)结束后立即测试脑于诱发电位(auditory brainstem responses,ABRs),取血清测TAC,制备耳蜗组织匀浆测NO的水平。所得结果如下:(1)无噪声对照组,动物听阈无明显变化;噪声刺激后生理盐水组,听阈上升的幅度明显高于α-硫辛酸组(P<0.05)。(2)噪声+生理盐水组,TAlC明显低于无噪声对照组(P<0.05);噪声+α-硫辛酸组同噪声+生理盐水组相比,TAC明显升高(P<0.05),同无噪声对照组相比,无显著性差异(P>0.05)。(3)噪声+生理盐水组,NO含量高于无噪声对照组(P<0.05);噪声+α-硫辛酸组同噪声+生理盐水组相比,NO含量明显减少(P<0.05),同无噪声对照组相比,差异无显著性(P>0.05)。上述结果提示,噪声刺激后血清TAC降低,耳蜗组织内NO含量增加;α-硫辛酸可通过抗氧化机制对噪声性听力损伤(noise induced hearing loss,NIHL)发挥保护作用。 展开更多
关键词 噪声性听力损伤 耳蜗 总抗氧化能力 一氧化氮 Α-硫辛酸
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诱生型一氧化氮合酶在正常豚鼠耳蜗内的分布 被引量:5
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作者 沈静 汤浩 +2 位作者 章晓芳 张合 张铁民 《中国医科大学学报》 CAS CSCD 北大核心 2000年第3期180-181,共2页
目的 :观察诱生型一氧化氮合酶 (inducible nitric oxide synthase,i NOS)在正常豚鼠耳蜗内的分布。方法 :以特异性 i NOS抗体 ,应用免疫组化 ABC法结合显微图像定量分析技术 ,在 10只正常豚鼠耳蜗冰冻切片上检测 i NOS。结果 :i NOS的... 目的 :观察诱生型一氧化氮合酶 (inducible nitric oxide synthase,i NOS)在正常豚鼠耳蜗内的分布。方法 :以特异性 i NOS抗体 ,应用免疫组化 ABC法结合显微图像定量分析技术 ,在 10只正常豚鼠耳蜗冰冻切片上检测 i NOS。结果 :i NOS的阳性免疫反应产物分布于耳蜗的血管纹、螺旋神经节细胞、Corti器的内、外毛细胞及神经纤维。结论 :正常豚鼠耳蜗有 i NOS表达 ,提示 i 展开更多
关键词 诱生型一氧化氮合酶 耳蜗 豚鼠 免疫组织化学
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