In the present study, RAPD technique was used to analyze the geneticrelationship between two cultured populations in china, oreochromis aureus and a niloticus,and the application of this technique in species identific...In the present study, RAPD technique was used to analyze the geneticrelationship between two cultured populations in china, oreochromis aureus and a niloticus,and the application of this technique in species identification was also investigated. Resultsrevealed that no DNA polymorphism was detected within the population of O.aureus andpolymorphism in varying degrees was observed among individuals. Similarity index andgenetic distance calculated have no remakable difference with data reported by others. RAPDanalysis using mixed samples was also tested in this experiment. In addition, someelectrophoregrams of amplification products also suggested the occurrence of geneticintrogression between the two cultured tilapia populations. Conclusively, RAPD technique isa rapid and efficient method for studying genetic polymorphism within and between differentpopulations, and RAPD markers can be used to identify different fish populations.展开更多
血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选...血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选应急通用桥接复苏液,已发展成为大趋势。我们对全血在严重失血患者的应用历史和现状做一介绍,并提出我国采用LTOWB作为首选应急通用血液的进一步论证和验证研究建议。展开更多
文摘In the present study, RAPD technique was used to analyze the geneticrelationship between two cultured populations in china, oreochromis aureus and a niloticus,and the application of this technique in species identification was also investigated. Resultsrevealed that no DNA polymorphism was detected within the population of O.aureus andpolymorphism in varying degrees was observed among individuals. Similarity index andgenetic distance calculated have no remakable difference with data reported by others. RAPDanalysis using mixed samples was also tested in this experiment. In addition, someelectrophoregrams of amplification products also suggested the occurrence of geneticintrogression between the two cultured tilapia populations. Conclusively, RAPD technique isa rapid and efficient method for studying genetic polymorphism within and between differentpopulations, and RAPD markers can be used to identify different fish populations.
文摘血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选应急通用桥接复苏液,已发展成为大趋势。我们对全血在严重失血患者的应用历史和现状做一介绍,并提出我国采用LTOWB作为首选应急通用血液的进一步论证和验证研究建议。
