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In silico prospective analysis of the medicinal plants activity on the CagA oncoprotein from Helicobacter pylori
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作者 Rafaela Viana Vieira Gabrielle Caroline Peiter +2 位作者 Fabrício Freire de Melo Ana Carla Zarpelon-Schutz Kádima Nayara Teixeira 《World Journal of Clinical Oncology》 2024年第5期653-663,共11页
BACKGROUND Colonization with Helicobacter pylori(H.pylori)has a strong correlation with gastric cancer,and the virulence factor CagA is implicated in carcinogenesis.Studies have been conducted using medicinal plants w... BACKGROUND Colonization with Helicobacter pylori(H.pylori)has a strong correlation with gastric cancer,and the virulence factor CagA is implicated in carcinogenesis.Studies have been conducted using medicinal plants with the aim of eliminating the pathogen;however,the possibility of blocking H.pylori-induced cell differentiation to prevent the onset and/or progression of tumors has not been addressed.This type of study is expensive and time-consuming,requiring in vitro and/or in vivo tests,which can be solved using bioinformatics.Therefore,prospective computational analyses were conducted to assess the feasibility of interaction between phenolic compounds from medicinal plants and the CagA oncoprotein.AIM To perform a computational prospecting of the interactions between phenolic compounds from medicinal plants and the CagA oncoprotein of H.pylori.METHODS In this in silico study,the structures of the phenolic compounds(ligands)kaempferol,myricetin,quercetin,ponciretin(flavonoids),and chlorogenic acid(phenolic acid)were selected from the PubChem database.These phenolic compounds were chosen based on previous studies that suggested medicinal plants as non-drug treatments to eliminate H.pylori infection.The three-dimensional structure model of the CagA oncoprotein of H.pylori(receptor)was obtained through molecular modeling using computational tools from the I-Tasser platform,employing the threading methodology.The primary sequence of CagA was sourced from GenBank(BAK52797.1).A screening was conducted to identify binding sites in the structure of the CagA oncoprotein that could potentially interact with the ligands,utilizing the GRaSP online platform.Both the ligands and receptor were prepared for molecular docking using AutoDock Tools 4(ADT)software,and the simulations were carried out using a combination of ADT and AutoDock Vina v.1.2.0 software.Two sets of simulations were performed:One involving the central region of CagA with phenolic compounds,and another involving the carboxy-terminus region of CagA with phenolic compounds.The receptor-ligand complexes were then analyzed using PyMol and BIOVIA Discovery Studio software.RESULTS The structure model obtained for the CagA oncoprotein exhibited high quality(C-score=0.09)and was validated using parameters from the MolProbity platform.The GRaSP online platform identified 24 residues(phenylalanine and leucine)as potential binding sites on the CagA oncoprotein.Molecular docking simulations were conducted with the three-dimensional model of the CagA oncoprotein.No complexes were observed in the simulations between the carboxy-terminus region of CagA and the phenolic compounds;however,all phenolic compounds interacted with the central region of the oncoprotein.Phenolic compounds and CagA exhibited significant affinity energy(-7.9 to-9.1 kcal/mol):CagA/kaempferol formed 28 chemical bonds,CagA/myricetin formed 18 chemical bonds,CagA/quercetin formed 16 chemical bonds,CagA/ponciretin formed 13 chemical bonds,and CagA/chlorogenic acid formed 17 chemical bonds.Although none of the phenolic compounds directly bound to the amino acid residues of the K-Xn-R-X-R membrane binding motif,all of them bound to residues,mostly positively or negatively charged,located near this region.CONCLUSION In silico,the tested phenolic compounds formed stable complexes with CagA.Therefore,they could be tested in vitro and/or in vivo to validate the findings,and to assess interference in CagA/cellular target interactions and in the oncogenic differentiation of gastric cells. 展开更多
关键词 CagA oncoprotein Phenolic compounds Helicobacter pylori In silico analyses Medicinal plants Prospective analysis
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人微管蛋白失稳剂Oncoprotein18原核表达载体的构建及表达
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作者 姚庆君 胡大海 +4 位作者 陈璧 徐明达 董茂龙 贾赤宇 王树森 《第四军医大学学报》 北大核心 2005年第3期210-213,共4页
目的:原核表达人Oncoprotein18(Op18)蛋白,为 制备抗Op18的mAb作准备.方法:从人皮肤组织中用RT PCR扩增Op18cDNA的全长编码序列,克隆入表达载体pR SETA中,构建Op18的高表达工程菌,并以IPTG诱导表达目 的蛋白,通过亲和层析法纯化表达的... 目的:原核表达人Oncoprotein18(Op18)蛋白,为 制备抗Op18的mAb作准备.方法:从人皮肤组织中用RT PCR扩增Op18cDNA的全长编码序列,克隆入表达载体pR SETA中,构建Op18的高表达工程菌,并以IPTG诱导表达目 的蛋白,通过亲和层析法纯化表达的Op18融合蛋白.结果: 酶切及测序鉴定证明,获得含有目的基因片段的重组质粒,表 达的Op18融合蛋白以可溶性的形式存在.结论:所获Op18 融合蛋白以可溶性的形式存在,为制备其mAb及进一步研究 Op18在创伤愈合及瘢痕形成中的作用打下了基础. 展开更多
关键词 微管蛋白失稳剂 oncoprotein18 原核表达
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Oncoprotein 18在肝癌侵袭转移过程中的作用 被引量:1
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作者 甘淋 段承刚 +3 位作者 龚舒 郭坤 舒宏 刘银坤 《世界华人消化杂志》 CAS 北大核心 2012年第23期2151-2156,共6页
目的:探讨原癌蛋白18(Oncoprotein 18,Op18)在肝癌侵袭转移过程中的作用机制.方法:采用R N A干扰,抑制人肝癌细胞HCCLM3中Op18的表达,RT-PCR和Westernb l o t评价干扰效率;通过细胞粘附分析、体外Transwell分析法检测Op18表达缺失后对HC... 目的:探讨原癌蛋白18(Oncoprotein 18,Op18)在肝癌侵袭转移过程中的作用机制.方法:采用R N A干扰,抑制人肝癌细胞HCCLM3中Op18的表达,RT-PCR和Westernb l o t评价干扰效率;通过细胞粘附分析、体外Transwell分析法检测Op18表达缺失后对HCCLM3细胞粘附、运动和侵袭能力的改变;RT-PCR和免疫组织化学分别在48例未伴转移的肝癌组织和48例伴转移的肝癌组织中解析Op18表达与肝癌侵袭转移的关系.结果:RT-PCR和Western blot结果显示:在HCCLM3细胞中行RNAi后Op18表达被有效抑制,抑制率达到80%以上;Op18表达缺失后,在不同时间点(20、40和60 min)实验组细胞粘附能力(0.616±0.057、0.740±0.0713和1.001±0.083)较阴性对照组(0.944±0.068、1.196±0.115和1.441±0.053)明显下降(P<0.05);Transwell实验结果提示:经RNAi后实验组细胞运动、侵袭能力(运动:0.145±0.011,侵袭0.127±0.008)较阴性对照组(运动:0.206±0.008,侵袭:0.168±0.012)显著降低(P<0.01);分别在伴转移和未伴转移的肝癌组织中检测Op18的表达,RT-PCR(Op18与GAPDH相对比值:0.560±0.128vs 0.414±0.086)和IHC(积分吸光度值为:624.771±100.032 vs 413.786±71.833)实验结果均提示Op18在伴转移的肝癌组织中的表达更高(P<0.01).结论:Op18在肝癌的侵袭转移过程中发挥重要作用. 展开更多
关键词 肝细胞癌 原癌蛋白18 RNA干扰 侵袭
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API2-MALT1 oncoprotein promotes lymphomagenesis via unique program of substrate ubiquitination and proteolysis 被引量:1
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作者 Shaun Rosebeck Megan S Lim +2 位作者 Kojo SJ Elenitoba-Johnson Linda MMcAllister-Lucas Peter C Lucas 《World Journal of Biological Chemistry》 CAS 2016年第1期128-137,共10页
Lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) is the most common extranodal B cell tumor and accounts for 8% of non-Hodgkin&#x02019;s lymphomas. Gastric MALT lymphoma is the best-studied example an... Lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) is the most common extranodal B cell tumor and accounts for 8% of non-Hodgkin&#x02019;s lymphomas. Gastric MALT lymphoma is the best-studied example and is a prototypical neoplasm that occurs in the setting of chronic inflammation brought on by persistent infection or autoimmune disease. Cytogenetic abnormalities are commonly acquired during the course of disease and the most common is chromosomal translocation t(11;18)(q21;q21), which creates the API2-MALT1 fusion oncoprotein. t(11;18)-positive lymphomas can be clinically aggressive and have a higher rate of dissemination than t(11;18)-negative tumors. Many cancers, including MALT lymphomas, characteristically exhibit deregulated over-activation of cellular survival pathways, such as the nuclear factor-&#x003ba;B (NF-&#x003ba;B) pathway. Molecular characterization of API2-MALT1 has revealed it to be a potent activator of NF-&#x003ba;B, which is required for API2-MALT1-induced cellular transformation, however the mechanisms by which API2-MALT1 exerts these effects are only recently becoming apparent. The API2 moiety of the fusion binds tumor necrosis factor (TNF) receptor associated factor (TRAF) 2 and receptor interacting protein 1 (RIP1), two proteins essential for TNF receptor-induced NF-&#x003ba;B activation. By effectively mimicking ligand-bound TNF receptor, API2-MALT1 promotes TRAF2-dependent ubiquitination of RIP1, which then acts as a scaffold for nucleating and activating the canonical NF-&#x003ba;B machinery. Activation occurs, in part, through MALT1 moiety-dependent recruitment of TRAF6, which can directly modify NF-&#x003ba;B essential modulator, the principal downstream regulator of NF-&#x003ba;B. While the intrinsic MALT1 protease catalytic activity is dispensable for this canonical NF-&#x003ba;B signaling, it is critical for non-canonical NF-&#x003ba;B activation. In this regard, API2-MALT1 recognizes NF-&#x003ba;B inducing kinase (NIK), the essential upstream regulator of non-canonical NF-&#x003ba;B, and cleaves it to generate a stable, constitutively active fragment. Thus, API2-MALT1 harnesses multiple unique pathways to achieve deregulated NF-&#x003ba;B activation. Emerging data from our group and others have also detailed additional gain-of-function activities of API2-MALT1 that extend beyond NF-&#x003ba;B activation. Specifically, API2-MALT1 recruits and subverts multiple other signaling factors, including LIM domain and actin-binding protein 1 (LIMA1) and Smac/DIABLO. Like NIK, LIMA1 represents a unique substrate for API2-MALT1 protease activity, but unlike NIK, its cleavage sets in motion a major NF-&#x003ba;B-independent pathway for promoting oncogenesis. In this review, we highlight the most recent results characterizing these unique and diverse gain-of-function activities of API2-MALT1 and how they contribute to lymphomagenesis. 展开更多
关键词 ONCOGENE Fusion oncoprotein Lymphoma Chromosomal translocation UBIQUITINATION Apoptosis Nuclear factor-κ B CASPASES
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MD Simulations of the P53 oncoprotein structure: the effect of the Arg273→His mutation on the DNA binding domain
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作者 Kholmirzo Kholmurodov Ermuhammad Dushanov Kenji Yasuoka 《Advances in Bioscience and Biotechnology》 2011年第5期330-335,共6页
A comparative molecular dynamics (MD) simulation study was performed on the p53 oncoprotein to investigate the effect of the Arg273His (R273H) mutation on the p53→DNA Binding Domain (DBD). The two p53 dimer structure... A comparative molecular dynamics (MD) simulation study was performed on the p53 oncoprotein to investigate the effect of the Arg273His (R273H) mutation on the p53→DNA Binding Domain (DBD). The two p53 dimer structures of the wild-type and mutant Arg273His (R273H) were simulated with the same thermodynamic and environmental parameters. The obtained results demonstrate that the induced Arg273His mutation has a considerable effect on the p53→DNA close contact interaction and changes the picture of hydrogen formation. The Arg273His mutation, in some cases, destroys the existing native hydrogen bond, but, in other cases, forms a strong p53→DNA hydrogen bond, which is not proper for the native protein. The MD simulation results illustrate some molecular mechanism of the conformational changes of the Arg273His key amino acid residue in the p53→DNA binding domain, which might be important for the understanding of the physiological functioning of the p53 protein and the origin of cancer. 展开更多
关键词 Molecular Dynamics Simulations P53 oncoprotein EFFECT of the R273H MUTATION DNA BINDING Domain
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EXPRESSIONS OF ESTROGEN OCCUPIED RECEPTOR(EoR)AND PROGESTERONE OCCUPIED RECEPTOR(PoR)AND C-erbB-2 ONCOPROTEIN IN HUMAN NASOPHARYNGEAL CARCINOMAS
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作者 谢佐福 林贤东 张竞时 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1995年第2期103-107,共5页
It is first reported here that estrogen occupied receptor(EoR)and progesterone occupied receptor (RoR)expressed in cancerous tissues (59.57% and 82.98% respectively)and morphologically normal epithlium(50 77.78% and ... It is first reported here that estrogen occupied receptor(EoR)and progesterone occupied receptor (RoR)expressed in cancerous tissues (59.57% and 82.98% respectively)and morphologically normal epithlium(50 77.78% and 70-88.89%respectively) in nasoplharyngeal carcinomas(NPCs)with insignificant difference(P>0.05).Positive rates of EoR and PoR increased greatly in clinical stage Ⅲ and Ⅳ, compared with in Ⅱ(P<0.05), and exhibited insignificant difference between female cases and male ones(P>0.05).Positive rate of C-erbB-2 was 19.15% in cancerous cells, and 9.68% in stage Ⅲand 66.67% in Ⅳin NPCs(P<0.05).Significant difference of C-erbB-2expression was observed between bilateral cervical lymph node metastasis(BCLM)and unilateral ones(P<0.05)but not for EoR or PoR(P>0.05)These findings suggest that EoR or PoR may be correlated with aggravation but not genesis and node metastasis in NPCs and that C-erbB-2may be correlated with aggravation and promotion of formation of node metastasis in NPCs. 展开更多
关键词 Female hormone receptors C-erbB-2oncoprotein Clinical stage Node metastasis Nasopharyngeal carcinoma.
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微管失稳剂oncoprotein18/stathmin基因在深Ⅱ度烧伤愈合前创面及增生性瘢痕中的表达 被引量:2
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作者 姚庆君 贾赤宇 +5 位作者 陈璧 卢宁 徐明达 刘新平 药立波 王树森 《中华烧伤杂志》 CAS CSCD 2003年第S1期18-20,共3页
目的 观察深Ⅱ度烧伤创面愈合前及愈合后增生性瘢痕组织中微管失稳剂oncopro tein18 stathmin(Op18)基因的表达。  方法 提取临床深Ⅱ度烧伤愈合前与愈合后不同时期增生性瘢痕中的总RNA,以GADPH基因表达为内参照 ,采用逆转录酶 多聚... 目的 观察深Ⅱ度烧伤创面愈合前及愈合后增生性瘢痕组织中微管失稳剂oncopro tein18 stathmin(Op18)基因的表达。  方法 提取临床深Ⅱ度烧伤愈合前与愈合后不同时期增生性瘢痕中的总RNA,以GADPH基因表达为内参照 ,采用逆转录酶 多聚酶链式反应 (RT PCR)方法 ,半定量检测上述基因表达水平。 结果 与正常组织比较 ,伤口愈合前Op18基因表达显著降低 (P <0.0 5 ),愈合后表达迅速增高 (P <0.0 5 ),在 4~ 32个月增生性瘢痕中Op18基因稳定高表达。  结论 在伤后早期Op18基因的表达水平降低与细胞增殖、组织修复的需要相适应 。 展开更多
关键词 烧伤 oncoprotein18/stathmin 微管 伤口愈合 瘢痕 基因表达调控
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Stathmin/Oncoprotein18(Op18)基因在人骨肉瘤中的表达及其意义
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作者 张大鹏 张圣洁 +1 位作者 于大淼 富勇 《现代生物医学进展》 CAS 2011年第19期3709-3712,共4页
目的:探讨stathmin/oncoprotein 18(Op18)基因在人骨肉瘤中的表达规律及意义。方法:分别采用免疫组化法和Western blot法检测10例正常骨组织和56例骨肉瘤中stathmin蛋白的表达。结果:免疫组化法检测stathmin蛋白在正常骨组织、低级别骨... 目的:探讨stathmin/oncoprotein 18(Op18)基因在人骨肉瘤中的表达规律及意义。方法:分别采用免疫组化法和Western blot法检测10例正常骨组织和56例骨肉瘤中stathmin蛋白的表达。结果:免疫组化法检测stathmin蛋白在正常骨组织、低级别骨肉瘤(G1级组)及高级别骨肉瘤(G2级组)中阳性表达率分别为20%、65%、100%。正常骨组织分别与G1级组、G2级组比较,均有显著性差异(P﹤0.05);G1级组与G2级组比较,有显著差异(P<0.05)。Western blot法检测显示,stathmin蛋白在正常骨组织、G1级组、G2级组表达相对值分别为(0.34±0.15)、(0.68±0.21)、(0.90±0.17)。正常骨组织分别与G1级级组、G2级组比较,差异均有显著性(P<0.01),G1级组与G2级组比较,差异有显著性(P<0.01)。结论:Stathmin在骨肉瘤中过表达,与骨肉瘤的发生及发展密切相关。 展开更多
关键词 骨肉瘤 stathmin/oncoprotein 18 免疫组化法 WESTERN BLOT
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Expression of oncoproteins c-fos and c jun in hypertrophic scars and chronic dermal ulcers and their regulation of basic fibroblast growth factor
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作者 付小兵 蒋礼先 +3 位作者 孙同柱 杨银辉 顾小曼 盛志勇 《Chinese Medical Journal》 SCIE CAS CSCD 2001年第8期69-73,109,共6页
Objective To explore the characteristics of oncoprotein expression of c-fos and c-jun in hypertrophic scars and chronic dermal ulcers and their regulation of basic fibroblast growth factor (bFGF). Methods Tissues of... Objective To explore the characteristics of oncoprotein expression of c-fos and c-jun in hypertrophic scars and chronic dermal ulcers and their regulation of basic fibroblast growth factor (bFGF). Methods Tissues of hypertrophic scars (n=8), chronic dermal ulcers (n=8) and normal skin (n=5) were taken from 21 patients with burns and chronic dermal ulcers in operation. The ABC immunohistochemical method was used to characterize the gene product expression of c-fos, c-jun and bFGF in the above tissues. Results In normal skin, both c-fos and c-jun protein expression and bFGF protein expression were observed. The signals of both oncoproteins were localized mainly in subcutaneous fibroblasts, but, positive expression of the bFGF protein was mainly in keratinocytes. In hypertrophic scars, positive expression of both oncoproteins could be found mainly in fibroblasts, but bFGF was mainly in fibroblasts and endothelial cells. In chronic dermal ulcers, endothelial cells, some of inflammatory cells and fibroblasts were positive for both of oncoproteins, but the expression of bFGF was only seen in fibroblasts and endothelial cells. Conclusions The results indicate that the interaction between both oncoproteins and bFGF exists, and the regulating action between protooncogenes and bFGF is a major course in wound healing. The different expressions of c-fos and c-jun gene products play an important role in regulate bFGF action, thus affecting wound healing. 展开更多
关键词 tumor gene · oncoprotein · basic fibroblas t growth factor · chronic dermal ulcer · hypertrophic scar ·t issue repair
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Transcriptional repression of hDaxx enhanced by adenovirus 12 E1B 55-kDa oncoprotein interacting with hDaxx 被引量:3
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作者 万艳平 吴移谋 +3 位作者 朱翠明 尹卫国 蔡恒玲 余敏君 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第5期753-757,共5页
Background Daxx has been identified as a nuclear protein that involves in apoptosis and transcriptional repression. Daxx co-localizes with the promyelocytic leukemia (PML) protein and regulates transcription. Human D... Background Daxx has been identified as a nuclear protein that involves in apoptosis and transcriptional repression. Daxx co-localizes with the promyelocytic leukemia (PML) protein and regulates transcription. Human Daxx (hDaxx) is a protein that functions as a transcriptional regulation through its interaction with some DNA-associated proteins. The aim of this study was to explore the transcriptional regulatory effect of hDaxx interacting with adenovirus (Ad) 12 E1B (Ad12E1B) 55-kDa oncoprotein Methods The co-localization of hDaxx-Ad12E1B or hDaxx-PML protein in the nucleus was observed under a confocal microscope Interaction of hDaxx and Ad12E1B was analyzed by yeast two-hybrid assay Direct binding of hDaxx and Ad12E1B was analyzed using coimmunoprecipitation and Western blot in vivo and in vitro The activity of a luciferase reporter gene, which was regulated by an hDaxx modulated thymidine kinase (TK) promoter, was detected in an automat luminometer Results Ad12E1B, which co-localized with hDaxx in the nuclei of G401-CC3 cells, disrupted the co-localization of hDaxx and PML in the PML oncogenic domains (PODs) hDaxx bound directly to Ad12E1B in vivo and in vitro hDaxx interacted with Ad12E1B along its full length Ad12E1B enhanced transcriptional repression activity of hDaxx Conclusion Ad12E1B disrupts the co-localization of hDaxx with PML in PODs and enhances transcriptional repression activity of hDaxx 展开更多
关键词 hDaxx · adenovirus type 12 · transcriptional repression · oncoprotein
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IMMUNOCYTOCHEMICAL ANALYSIS OF ras RELATED PROTEINS WITH ANTISERUM AGAINST PEPTIDE FRAGMENT OF ras ONCOPROTEIN P21 (56—66) IN HUMAN CANCER TISSUES
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作者 张红 陈韵能 +3 位作者 钮经义 李士谔 林原 刘彤华 《Chinese Science Bulletin》 SCIE EI CAS 1989年第12期1049-1053,共5页
The c-ras and its product P21 have been shown to play an important role in the initiation and development of some malignancies. The activation of c-ras may be associated with two mechanisms: (ⅰ) a point mutation of t... The c-ras and its product P21 have been shown to play an important role in the initiation and development of some malignancies. The activation of c-ras may be associated with two mechanisms: (ⅰ) a point mutation of the codon at position 12 or 61 of the genome leading to a single amino acid alteration in the corresponding product P21; (ⅱ) enhanced expression of ras family encode proteins with 展开更多
关键词 RAS oncoprotein P21 immunocytochemical analysis human primary cancer adjacent tissue.
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Immune response modulation in inflammatory bowel diseases by Helicobacter pylori infection
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作者 Gabriella Feilstrecker Balani Mariana dos Santos Cortez +3 位作者 Jayme Euclydes Picasky da Silveira Freitas Fabrício Freire de Melo Ana Carla Zarpelon-Schutz Kádima Nayara Teixeira 《World Journal of Gastroenterology》 SCIE CAS 2023年第30期4604-4615,共12页
Many studies point to an association between Helicobacter pylori(H.pylori)infection and inflammatory bowel diseases(IBD).Although controversial,this association indicates that the presence of the bacterium somehow aff... Many studies point to an association between Helicobacter pylori(H.pylori)infection and inflammatory bowel diseases(IBD).Although controversial,this association indicates that the presence of the bacterium somehow affects the course of IBD.It appears that H.pylori infection influences IBD through changes in the diversity of the gut microbiota,and hence in local chemical characteristics,and alteration in the pattern of gut immune response.The gut immune response appears to be modulated by H.pylori infection towards a less aggressive inflammatory response and the establishment of a targeted response to tissue repair.Therefore,a T helper 2(Th2)/macrophage M2 response is stimulated,while the Th1/macrophage M1 response is suppressed.The immunomodulation appears to be associated with intrinsic factors of the bacteria,such as virulence factors-such oncogenic protein cytotoxin-associated antigen A,proteins such H.pylori neutrophil-activating protein,but also with microenvironmental changes that favor permanence of H.pylori in the stomach.These changes include the increase of gastric mucosal pH by urease activity,and suppression of the stomach immune response promoted by evasion mechanisms of the bacterium.Furthermore,there is a causal relationship between H.pylori infection and components of the innate immunity such as the NLR family pyrin domain containing 3 inflammasome that directs IBD toward a better prognosis. 展开更多
关键词 Cytotoxin-associated antigen A oncoprotein Gut microbiota Helicobacter pylori Helicobacter pylori neutrophilactivating protein Immunological modulation Inflammatory bowel disease NLR family pyrin domain containing 3 inflammasome
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麻黄果实总黄酮含量的光学分析
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作者 王书华 邓立宏 《神经药理学报》 2000年第1期21-21,共1页
近年来我们对麻黄果实进行了较为全面的研究,结果表明该果实具有抗凝血、降血压等作用。为此我们采用以芦丁为标准,用分光光度法对其总黄酮类物质含量进行了测定。
关键词 IMMUNOHISTOCHEMISTRY Metastaric CARCINOMA bcl-2 C-MYC oncoprotein Expression
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肺癌癌基因蛋白产物同步检测的对比分析 被引量:54
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作者 赵彤 朱梅刚 +3 位作者 黄宗义 张亚历 张素娟 李梅芳 《癌症》 SCIE CAS CSCD 北大核心 1995年第1期13-15,共3页
用免疫组织化学方法对74例肺癌连续切片组织同步进行了ras、C-myc、P53三种癌基因蛋白产物的检测。结果显示rasP21、C-mycP62、和P53阳性表达率分别为71.6%、64.8%和33.8%。而癌旁支气管... 用免疫组织化学方法对74例肺癌连续切片组织同步进行了ras、C-myc、P53三种癌基因蛋白产物的检测。结果显示rasP21、C-mycP62、和P53阳性表达率分别为71.6%、64.8%和33.8%。而癌旁支气管粘膜及腺体分别为16.1%、22.6%和0。P21和P62之间的阳性率无统计学差别(P>0.05),表明这二个癌基因在肺癌发生发展中具有同等的重要性。有两种以上癌基因蛋白同时表达阳性者有46例(62.2%),10例(21.7%)同时有P21、P62和P53共同表达,结果提示肺癌在发生发展过程中多需要两种或两种以上癌基因作用的结果。 展开更多
关键词 肺肿瘤 癌基因 癌基因蛋白 免疫组织化学 病理学
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HPV16型-E6、E7在食管鳞癌组织与非癌组织中的表达 被引量:21
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作者 许春雷 千新来 +2 位作者 周小山 赵清正 李艳春 《癌症》 SCIE CAS CSCD 北大核心 2004年第2期165-168,共4页
背景与目的:越来越多的证据表明高危型人乳头状瘤病毒(humanpapillomavirus,HPV),特别是HPV16型与多种肿瘤发生、发展密切相关。本研究旨在分析人乳头状瘤病毒HPV16型E6、E7在食管正常组织、不典型增生组织和癌组织中的表达,以探讨HPV1... 背景与目的:越来越多的证据表明高危型人乳头状瘤病毒(humanpapillomavirus,HPV),特别是HPV16型与多种肿瘤发生、发展密切相关。本研究旨在分析人乳头状瘤病毒HPV16型E6、E7在食管正常组织、不典型增生组织和癌组织中的表达,以探讨HPV16型在食管鳞癌发生、发展中的生物学意义。方法:采用PicTureTM免疫组织化学方法对70例食管癌切除标本上、下切缘的正常粘膜上皮组织、43例不典型增生组织以及18例癌组织中的HPV16型E6、E7蛋白表达情况进行研究。结果:E6蛋白阳性率在正常食管粘膜上皮组织中为59.3%,在上皮不典型增生组织中为88.4%,在癌组织中为83.3%;E7蛋白在上述3种组织中的阳性率分别为62.1%、90.7%和88.9%。与正常食管粘膜上皮相比,上皮不典型增生组织和癌组织中E6、E7蛋白表达均有显著性差异(P<0.05)。HPV16型E6、E7蛋白在正常食管粘膜组织中同时表达即同步率为25.7%,而在上皮不典型增生组织和癌组织中二者同步率分别为88.3%和83.3%。结论:HPV16型E6、E7蛋白与食管鳞癌发生、发展密切相关,二者协同作用可能是食管鳞癌发生、发展的重要因素之一。 展开更多
关键词 食管鳞癌 人乳头状瘤病毒16型 E6 E7 免疫组织化学
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癌基因蛋白ras p21、甲胎蛋白在实验性大鼠肛癌前病变中的表达 被引量:19
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作者 郑杰 武忠弼 +2 位作者 阮幼冰 杨木兰 刘冰 《临床与实验病理学杂志》 CAS CSCD 北大核心 1995年第2期133-136,共4页
应用免疫组化技术,检测了rasp21、AFP在二乙基亚硝胺(DENA)诱发的大鼠肝癌前病变中的表达。结果显示:诱癌早期肝小叶周边区即出现p21、AFP表达阳性细胞,随诱癌过程进行,阳性细胞增多并主要分布在变异肝细胞灶... 应用免疫组化技术,检测了rasp21、AFP在二乙基亚硝胺(DENA)诱发的大鼠肝癌前病变中的表达。结果显示:诱癌早期肝小叶周边区即出现p21、AFP表达阳性细胞,随诱癌过程进行,阳性细胞增多并主要分布在变异肝细胞灶和结节中,且常见两者同时表达的现象。由此提示,两者与大鼠肝癌发生有密切关系,p21过度表达直接参与了大鼠肝癌的启动和演进。同时表达AFP、p21的细胞和病变是较具特异性的癌前病变。p2l与AFP的联合表达可为人类肝癌提供一个参考诊断标志。 展开更多
关键词 癌基因蛋白 癌前病变 肝肿瘤 甲胎蛋白
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胃癌组织中PCNA评分和Bc1-2、p53蛋白表达及相互关系 被引量:8
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作者 杨竹林 李永国 +3 位作者 黄生福 钟德许 庄赞根 吕方 《中国肿瘤临床》 CAS CSCD 北大核心 1997年第11期811-814,共4页
应用ABC免疫组化法研究50例胃癌组织中PCNA评分和Bc1-2、p53蛋白表达。50例胃癌PCNA评分均数为2.400×1.010和Bc1-2、p53蛋白阳性表达率分别为44%及34%;高分化、组织学分级Ⅰ级和未见淋巴结转移胃癌PCNA评分均数和P53蛋白阳... 应用ABC免疫组化法研究50例胃癌组织中PCNA评分和Bc1-2、p53蛋白表达。50例胃癌PCNA评分均数为2.400×1.010和Bc1-2、p53蛋白阳性表达率分别为44%及34%;高分化、组织学分级Ⅰ级和未见淋巴结转移胃癌PCNA评分均数和P53蛋白阳性表达率均明显低于低或未分化、组织学分级Ⅲ级和伴淋巴结转移病例;高分化和组织学分级Ⅰ级胃癌Bc1-2蛋白阳性表达率均明显高于未分化和组织学分级Ⅲ级胃癌;Bc1-2蛋白阳性病例PCNA评分均数明显低于Bcl-2蛋白阴性病例,P53蛋白阳性病例PCNA评分明显高于P53蛋白阴性病例。提示PCNA评分和Bc1-2、p53蛋白表达与胃癌发生发展有密切关系,PCNA评分高和p53蛋白阳性病例可能恶性度高和预后差,而Bc1-2蛋白阳性病例恶性度低和预后较好。 展开更多
关键词 胃肿瘤 增殖细胞核抗原 基因蛋白 免疫组织化学
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食管鳞癌中人表皮生长因子受体Her-2/neu蛋白的过表达及预后意义 被引量:10
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作者 邓勇军 李海滨 +3 位作者 沈剑 杨鸿生 洪志鹏 李定彪 《中国现代医学杂志》 CAS CSCD 北大核心 2009年第24期3726-3730,共5页
目的分析食管鳞癌中Her-2/neu蛋白过表达的临床病理意义及其对预后的影响,探讨以Her-2/neu单抗对过表达Her-2/neu蛋白的食管鳞癌患者进行靶向治疗的可行性。方法应用Hercep Test试剂盒,对94例外科切除、病理诊断为食管鳞癌的标本中Her-2... 目的分析食管鳞癌中Her-2/neu蛋白过表达的临床病理意义及其对预后的影响,探讨以Her-2/neu单抗对过表达Her-2/neu蛋白的食管鳞癌患者进行靶向治疗的可行性。方法应用Hercep Test试剂盒,对94例外科切除、病理诊断为食管鳞癌的标本中Her-2/neu蛋白的表达水平进行检测,并分析Her-2/neu蛋白的过表达与临床病理特征的关系以及与患者预后的关系。结果HER-2/neu蛋白在癌旁组织及正常食管组织中的表达为阴性,在94例食管鳞癌中的过表达率为20.2%(19/94),其过表达与患者的性别、年龄、肿瘤部位、组织学分化程度、临床分期均无明显相关性(P>0.05),而与肿瘤浸润深度(T分期)以及淋巴结转移(N分期)具有一定的相关性,在T3+T4期的肿瘤组织中的过表达率明显高于T1+T2期的肿瘤组织中的过表达率(30.0%vs.13.0%),差异有统计学意义(χ2=4.136,P=0.039);在N1期肿瘤组织中的过表达率明显高于在N0期肿瘤组织中的过表达率(26.8%vs.10.6%),差异有统计学意义(χ2=3.711,P=0.045);生存分析显示HER-2/neu蛋白过表达组的总生存率低于非过表达组,差异有统计学意义(χ2=6.258,P=0.017),而两组的无瘤生存率差异无统计学意义(χ2=0.258,P=0.605);Cox模型多因素分析显示HER-2/neu基因过表达是影响患者总生存率的有意义的预后因素之一。结论HER-2/neu基因过表达与食管鳞癌的浸润转移及预后密切相关,HER-2/neu蛋白过表达检测可作为判断食管鳞癌生物学行为和预测预后的参考指标之一,并为HER-2/neu过表达的病例应用HER-2/neu单克隆抗体进行靶向治疗提供依据。 展开更多
关键词 食管肿瘤 表皮生长因子受体-2 过表达 预后
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腺病毒E1B55ku癌蛋白打破hDaxx与PML在细胞核的共定位 被引量:8
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作者 万艳平 谭立志 +3 位作者 刘传爱 吴移谋 Colosimo April 廖代清 《微生物学杂志》 CAS CSCD 2001年第4期46-48,共3页
利用间接免疫荧光试验 ,通过Confocal激光扫描生物荧光显微镜和图像软件分析腺病毒 (adenovirus,Ad)E1B 5 5ku癌蛋白 (AdE1B 5 5ku)与人Daxx(humanDaxx ,hDaxx)在细胞核的定位关系 ,研究它对早幼粒细胞性白血病蛋白 (promyelocyticleuke... 利用间接免疫荧光试验 ,通过Confocal激光扫描生物荧光显微镜和图像软件分析腺病毒 (adenovirus,Ad)E1B 5 5ku癌蛋白 (AdE1B 5 5ku)与人Daxx(humanDaxx ,hDaxx)在细胞核的定位关系 ,研究它对早幼粒细胞性白血病蛋白 (promyelocyticleukemiaprotein ,PML)与hDaxx在细胞核定位关系的影响。实验结果表明 ,AdE1B 5 5ku与hDaxx共定位细胞核 ,并打破hDaxx与PML共定位于细胞核PML致癌结构域 (PMLoncogenicdomains,PODs) 展开更多
关键词 腺病毒E1B55ku癌蛋白 HDAXX PML 细胞核 共定位 感染
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星形细胞瘤p16、Rb、cyclinD1、EGFR表达和DNA含量测定 被引量:10
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作者 范智勇 杨鑫 +3 位作者 赵玉宁 曹晓哲 朱峰 黄克斌 《临床与实验病理学杂志》 CAS CSCD 2000年第1期44-47,共4页
目的 :探讨 p16、Rb、cyclinD1和EGFR在星形细胞肿瘤发生、发展中的作用及其与预后的关系。 方法 :免疫组化染色采用即用型S P法 ,用图像分析系统对 80例星形细胞肿瘤瘤细胞进行DNA含量测定。结果 :80例星形细胞肿瘤 :p16、Rb、cyclinD1... 目的 :探讨 p16、Rb、cyclinD1和EGFR在星形细胞肿瘤发生、发展中的作用及其与预后的关系。 方法 :免疫组化染色采用即用型S P法 ,用图像分析系统对 80例星形细胞肿瘤瘤细胞进行DNA含量测定。结果 :80例星形细胞肿瘤 :p16、Rb、cyclinD1和EGFR的阳性率分别为 43 8%、71 3%、5 5 0 %和 85 0 % ,4种蛋白阳性率及DNA含量与组织学分级均有统计学意义 ;p16、Rb、cyclinD1阳性率和瘤细胞DNA含量与预后有关 ,EGFR阳性率与预后无关 ;Rb失表达时 ,p16表达增强。 结论 :p16和Rb在抑制星形细胞肿瘤的生长及EGFR和cyclinD1促使其生长中起着重要作用 ,p16表达同DNA含量相结合评估星形细胞瘤预后有一定价值。 展开更多
关键词 脑肿瘤 星形细胞瘤 癌基因蛋白 DNA含量
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