基于产出和性能的公路合同OPRC (Output and Performance Based Road Contract,简称OPRC合同)。近年来,OPRC合同先后在印度、新西兰和利比里亚等国纷纷试点成功并推广,取得了不错效果。但国内还没有将OPRC合同用于公路养护工程的实例,...基于产出和性能的公路合同OPRC (Output and Performance Based Road Contract,简称OPRC合同)。近年来,OPRC合同先后在印度、新西兰和利比里亚等国纷纷试点成功并推广,取得了不错效果。但国内还没有将OPRC合同用于公路养护工程的实例,安徽省公路养护是国内首度采用OPRC合同模式。OPRC项目主要是强调了承包商设计和建后维护能力,最突出的特点是大部分款项不按照实物工程量计量支付,而是按照ORRC合同条款设计绩效给予支付。展开更多
基于产出和性能的公路合同OPRC(Output and Performance Based Road Contract,简称OPRC合同),近年来,先后在印度、新西兰和利比里亚等国纷纷试点成功并推广,取得了不错的效果。安徽省公路养护工程是国内首度采用OPRC合同模式的工程,在...基于产出和性能的公路合同OPRC(Output and Performance Based Road Contract,简称OPRC合同),近年来,先后在印度、新西兰和利比里亚等国纷纷试点成功并推广,取得了不错的效果。安徽省公路养护工程是国内首度采用OPRC合同模式的工程,在借鉴其他各国成功经验的基础上,将OPRC合同的精髓本土化,既推进道路养护市场化的进程,又不与中国和本省的客观实际脱节。文章对此展开研究。展开更多
The present study identified the properties of porins in the outer membrane in Pseudomonas aeruginosa,and showed the role of outer membrane in determining imipenem diffusion in Pseudomonas a...The present study identified the properties of porins in the outer membrane in Pseudomonas aeruginosa,and showed the role of outer membrane in determining imipenem diffusion in Pseudomonas aeruginosa The molecular weight of the major outer membrane protein was analyzed by SDS PAGE The purification of the porins in Pseudomonas aeruginosa was achieved by DEAE ion exchange HPLC The purified outer membrane proteins were reconstituted with phosphatidylcholine and dicetylphosphate into membrane vesicles, and were tested by the liposomes swelling method for the diffusion of imipenem The permeability assay showed that OprC (70 kD), OprD 2 (46kD), and OprE(43 kD) were the channel forming proteins But only OprD 2 was thought to be the likely route of imipenem diffusion展开更多
文摘基于产出和性能的公路合同OPRC (Output and Performance Based Road Contract,简称OPRC合同)。近年来,OPRC合同先后在印度、新西兰和利比里亚等国纷纷试点成功并推广,取得了不错效果。但国内还没有将OPRC合同用于公路养护工程的实例,安徽省公路养护是国内首度采用OPRC合同模式。OPRC项目主要是强调了承包商设计和建后维护能力,最突出的特点是大部分款项不按照实物工程量计量支付,而是按照ORRC合同条款设计绩效给予支付。
文摘基于产出和性能的公路合同OPRC(Output and Performance Based Road Contract,简称OPRC合同),近年来,先后在印度、新西兰和利比里亚等国纷纷试点成功并推广,取得了不错的效果。安徽省公路养护工程是国内首度采用OPRC合同模式的工程,在借鉴其他各国成功经验的基础上,将OPRC合同的精髓本土化,既推进道路养护市场化的进程,又不与中国和本省的客观实际脱节。文章对此展开研究。
文摘The present study identified the properties of porins in the outer membrane in Pseudomonas aeruginosa,and showed the role of outer membrane in determining imipenem diffusion in Pseudomonas aeruginosa The molecular weight of the major outer membrane protein was analyzed by SDS PAGE The purification of the porins in Pseudomonas aeruginosa was achieved by DEAE ion exchange HPLC The purified outer membrane proteins were reconstituted with phosphatidylcholine and dicetylphosphate into membrane vesicles, and were tested by the liposomes swelling method for the diffusion of imipenem The permeability assay showed that OprC (70 kD), OprD 2 (46kD), and OprE(43 kD) were the channel forming proteins But only OprD 2 was thought to be the likely route of imipenem diffusion