Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains...Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains unconfirmed.In our study,gene expression data of ESCC and clinical information from public databases were downloaded.Through LASSO-Cox regression analysis,a risk score(RS)signature map of prognosis was constructed and performed external verification with the GSE53625 cohort.The ESTIMATE,xCell,CIBERSORT,TIMER,and ImmuCellAI algorithms were employed to analyze infiltrating immune cells and generate an immune microenvironment(IM).Afterward,functional enrichment analysis clarified the underlying mechanism of the model.Nomogram was utilized for forecasting the survival rate of individual ESCC cases.As a result,we successfully constructed an OS-related genes(OSRGs)model and found that the survival rate of high-risk groups was lower than that of low-risk groups.The AUC of the ROC verified the strong prediction performance of the signal in these two cohorts further.According to independent prognostic analysis,the RS was identified as an independent risk factor for ESCC.The nomogram and follow-up data revealed that the RS possesses favorable predictive value for the prognosis of ESCC patients.qRT-PCR detection demonstrated increased expression of MPC1,COX6C,CYB5R3,CASP7,and CYCS in esophageal cancer patients.In conclusion,we have constructed an OSRGs model for ESCC to predict patients’prognosis,offering a novel insight into the potential application of the OSRGs model in ESCC.展开更多
目的分析脊髓小脑共济失调8型(Spinocerebellar ataxia type 8,SCA8)患者的基因检测结果,总结有效治疗方法。方法对1例拟诊SCA8患者的基因检测结果和治疗方法作回顾性分析。结果18岁男性患者,因“步态不稳12年”就诊,临床表现为行走不...目的分析脊髓小脑共济失调8型(Spinocerebellar ataxia type 8,SCA8)患者的基因检测结果,总结有效治疗方法。方法对1例拟诊SCA8患者的基因检测结果和治疗方法作回顾性分析。结果18岁男性患者,因“步态不稳12年”就诊,临床表现为行走不稳、剪刀步态、双下肢肌张力增高。抽取患者及其父母的外周静脉血,行遗传性共济失调Panel基因检测、全外显子测序以及Sanger测序验证,基因检测结果显示:患者的ATXN8/ATXN8OS基因CAG重复分别是18次和68次,其母亲ATXN8/ATXN8OS基因CAG重复分别是24次和67次,患者父亲基因检测结果未见异常。结合患者临床症状、生物信息学软件预测结果,明确诊断为SCA8。为改善患者双下肢痉挛症状,实施选择性脊神经背根切断术(SDR)及脊神经根粘连松解术治疗,并进行术后康复锻炼。术后6个月,患者剪刀步态明显改善,行走基本平稳,MAS评分、VAS评分、坐位平衡及立位平衡均有改善。结论ATXN8/ATXN8OS基因串联重复区的CAG异常重复扩增可导致SCA8,临床表现为痉挛性步态不稳,结合基因检测结果可明确诊断,SDR可有效改善SCA8患者的痉挛性步态。展开更多
The coal metamorphism in Central Hunan pro-vides valuable information about hydrothermal activity and water/rock reactions.Learning how to collect age data on hydrothermal fluid systems is necessary for understanding ...The coal metamorphism in Central Hunan pro-vides valuable information about hydrothermal activity and water/rock reactions.Learning how to collect age data on hydrothermal fluid systems is necessary for understanding the history and genetic mechanisms of large-scale coal-generated graphite deposits.The Shihangli graphite deposit,formed by significant siliceous hydrothermal alteration,is the most distinctive in Central Hunan.Re–Os dating of pyrite from the Shihangli graphite deposit demonstrates that the coal-generated graphite mineraliza-tion age is-127.6±3.8 Ma.Based on in-situ mineral analysis,the hydrothermal pyrite in the Shihangli graphite deposit is mostly enriched in Sb,As,Au,W,Ag,Cu,Pb,and Zn.Based on the pyrite Re–Os isochron,the initial(^(187)Os/^(188)Os)values of pyrite were 1.03±0.24 and the Os(t)values varied from 571.8 to 755.1.Pyrite from the Shihangli graphite deposit comprises a Pb isotope composition similar to that of the Madiyi Formation bulk rock and stibnite from the Xikuangshan Sb deposit.Based on the Re–Os,Sr,S,and Pb isotopic compositions of sul-fides in the graphite and Sb deposits in Central Hunan,the Madiyi Formation was likely the primary source of ore-forming elements(Sb,Au,and As).The Re–Os and Pb isotope compositions of pyrite most likely reflect when large-scale fluid migration and coal-generated graphite mineralization occurred in Central Hunan.展开更多
A secure operating system in the communication network can provide the stable working environment,which ensures that the user information is not stolen.The micro-kernel operating system in the communication network re...A secure operating system in the communication network can provide the stable working environment,which ensures that the user information is not stolen.The micro-kernel operating system in the communication network retains the core functions in the kernel,and unnecessary tasks are implemented by calling external processes.Due to the small amount of code,the micro-kernel architecture has high reliability and scalability.Taking the microkernel operating system in the communication network prototype VSOS as an example,we employ the objdump tool to disassemble the system source code and get the assembly layer code.On this basis,we apply the Isabelle/HOL,a formal verification tool,to model the system prototype.By referring to the mathematical model of finite automata and taking the process scheduling module as an example,the security verification based on the assembly language layer is developed.Based on the Hoare logic theory,each assembly statement of the module is verified in turn.The verification results show that the scheduling module of VSOS has good functional security,and also show the feasibility of the refinement framework.展开更多
基金Natural Science Foundation of Ningbo(Grant No.2021J261).
文摘Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains unconfirmed.In our study,gene expression data of ESCC and clinical information from public databases were downloaded.Through LASSO-Cox regression analysis,a risk score(RS)signature map of prognosis was constructed and performed external verification with the GSE53625 cohort.The ESTIMATE,xCell,CIBERSORT,TIMER,and ImmuCellAI algorithms were employed to analyze infiltrating immune cells and generate an immune microenvironment(IM).Afterward,functional enrichment analysis clarified the underlying mechanism of the model.Nomogram was utilized for forecasting the survival rate of individual ESCC cases.As a result,we successfully constructed an OS-related genes(OSRGs)model and found that the survival rate of high-risk groups was lower than that of low-risk groups.The AUC of the ROC verified the strong prediction performance of the signal in these two cohorts further.According to independent prognostic analysis,the RS was identified as an independent risk factor for ESCC.The nomogram and follow-up data revealed that the RS possesses favorable predictive value for the prognosis of ESCC patients.qRT-PCR detection demonstrated increased expression of MPC1,COX6C,CYB5R3,CASP7,and CYCS in esophageal cancer patients.In conclusion,we have constructed an OSRGs model for ESCC to predict patients’prognosis,offering a novel insight into the potential application of the OSRGs model in ESCC.
文摘目的分析脊髓小脑共济失调8型(Spinocerebellar ataxia type 8,SCA8)患者的基因检测结果,总结有效治疗方法。方法对1例拟诊SCA8患者的基因检测结果和治疗方法作回顾性分析。结果18岁男性患者,因“步态不稳12年”就诊,临床表现为行走不稳、剪刀步态、双下肢肌张力增高。抽取患者及其父母的外周静脉血,行遗传性共济失调Panel基因检测、全外显子测序以及Sanger测序验证,基因检测结果显示:患者的ATXN8/ATXN8OS基因CAG重复分别是18次和68次,其母亲ATXN8/ATXN8OS基因CAG重复分别是24次和67次,患者父亲基因检测结果未见异常。结合患者临床症状、生物信息学软件预测结果,明确诊断为SCA8。为改善患者双下肢痉挛症状,实施选择性脊神经背根切断术(SDR)及脊神经根粘连松解术治疗,并进行术后康复锻炼。术后6个月,患者剪刀步态明显改善,行走基本平稳,MAS评分、VAS评分、坐位平衡及立位平衡均有改善。结论ATXN8/ATXN8OS基因串联重复区的CAG异常重复扩增可导致SCA8,临床表现为痉挛性步态不稳,结合基因检测结果可明确诊断,SDR可有效改善SCA8患者的痉挛性步态。
基金the National Key Research and Development Program of China(2016YFC0600207,2014CB440904)National Natural Science Foundation of China(Nos.42062006,41962007)the Integrated Exploration Project of China Geological Survey(No.12120114034501).
文摘The coal metamorphism in Central Hunan pro-vides valuable information about hydrothermal activity and water/rock reactions.Learning how to collect age data on hydrothermal fluid systems is necessary for understanding the history and genetic mechanisms of large-scale coal-generated graphite deposits.The Shihangli graphite deposit,formed by significant siliceous hydrothermal alteration,is the most distinctive in Central Hunan.Re–Os dating of pyrite from the Shihangli graphite deposit demonstrates that the coal-generated graphite mineraliza-tion age is-127.6±3.8 Ma.Based on in-situ mineral analysis,the hydrothermal pyrite in the Shihangli graphite deposit is mostly enriched in Sb,As,Au,W,Ag,Cu,Pb,and Zn.Based on the pyrite Re–Os isochron,the initial(^(187)Os/^(188)Os)values of pyrite were 1.03±0.24 and the Os(t)values varied from 571.8 to 755.1.Pyrite from the Shihangli graphite deposit comprises a Pb isotope composition similar to that of the Madiyi Formation bulk rock and stibnite from the Xikuangshan Sb deposit.Based on the Re–Os,Sr,S,and Pb isotopic compositions of sul-fides in the graphite and Sb deposits in Central Hunan,the Madiyi Formation was likely the primary source of ore-forming elements(Sb,Au,and As).The Re–Os and Pb isotope compositions of pyrite most likely reflect when large-scale fluid migration and coal-generated graphite mineralization occurred in Central Hunan.
基金This work was supported in part by the Natural Science Foundation of Jiangsu Province under grant No.BK20191475the fifth phase of“333 Project”scientific research funding project of Jiangsu Province in China under grant No.BRA2020306the Qing Lan Project of Jiangsu Province in China under grant No.2019.
文摘A secure operating system in the communication network can provide the stable working environment,which ensures that the user information is not stolen.The micro-kernel operating system in the communication network retains the core functions in the kernel,and unnecessary tasks are implemented by calling external processes.Due to the small amount of code,the micro-kernel architecture has high reliability and scalability.Taking the microkernel operating system in the communication network prototype VSOS as an example,we employ the objdump tool to disassemble the system source code and get the assembly layer code.On this basis,we apply the Isabelle/HOL,a formal verification tool,to model the system prototype.By referring to the mathematical model of finite automata and taking the process scheduling module as an example,the security verification based on the assembly language layer is developed.Based on the Hoare logic theory,each assembly statement of the module is verified in turn.The verification results show that the scheduling module of VSOS has good functional security,and also show the feasibility of the refinement framework.
